Papers by Prof. Dr. Mohamed Salah Ayyat

Journal of Applied Aquaculture, 2011
Protein in the diet (25 Vs 31%), stocking rate (100 Vs 200 fish 10 per m 3 ), and inclusion rate ... more Protein in the diet (25 Vs 31%), stocking rate (100 Vs 200 fish 10 per m 3 ), and inclusion rate of pantothenic acid, vitamin B5, (10 Vs 20 mg/kg diet) were compared for their effects on blood plasma protein, albumin, urea-N, creatinine, plasma transaminase enzymes (AST and ALT), thyroxin (T 3 ), and growth in Oreochromis niloticus juveniles. Blood total protein, albumin, ALT, 15 T 3 , and growth were significantly (P < 0.001) higher in fish groups fed the high protein diet, while urea-N concentration in blood was significantly (P < 0.001) lower. Final body weight and daily weight gain were lower and food conversion rate was higher at the higher stocking density. Blood total protein, albumin, ALT, and T 3 were 20 lower in fish groups reared at high stocking density. Body weight and daily gain was significantly higher at the higher pantothenic acid inclusion level, while feed conversion ratio was lower. Blood total protein, albumin, ALT, and T 3 were also elevated. The highest income to the system was from the group fed high protein, reared 25 at low stocking density and fed diets supplemented with 10 mg vitamin B5.

The effect of organic selenium, prebiotics, or probiotics on productive performances, blood bioch... more The effect of organic selenium, prebiotics, or probiotics on productive performances, blood biochemistry, and carcass characteristics of growing rabbits was studied throughout summer and winter seasons. In an 8-week feeding trial, a total of 100 New Zealand White rabbits were randomly distributed to 10 groups. Two seasons (winter and summer) and five diets fortified with 0 (control), 0.03 mg selenium, 3 g Bio-Mos®, 1 g Bactocell® (1 × 1010CFU) or 3 g yeast/kg diet were used in 2 × 5 factorial design. Results indicated that growth performance, feed intake (FI), and blood components (red blood cells [RBCs], serum total protein [TP], globulin [GLOB],albumin [ALB]) decreased significantly in rabbits reared during summer than in those during winter. In contrast, white blood cells,urea-N, creatinine, alanine transaminase [ALT], and aspartate transaminase [AST] increased significantly in summer. However,growth performance indices, FI, blood hemoglobin, RBCs, TP, ALB, and GLOB increased significantly in rabbits when fed thetested feed additives. The respiration rate, rectal temperature, and heart rate of the animals were significantly decreased with all feedadditives. Adjusted weight of carcass, liver, kidney fat, and carcass cuts were not affected by feed additives. Final margin and marginefficiency increased in rabbit fed diets supplemented with feed additives than those fed the basal diet without any supplementation.Results of the current study concluded that a supplementation of rabbit diets with organic selenium, probiotics, and prebiotics canpromote rabbit performance during mild weather and also alleviate the adverse impact of heat stress during summer season.
World Rabbit Science, 2010

SuRiZMARY -Blood and milk samples were collected from New Zealand White WZW) and Californian (Cal... more SuRiZMARY -Blood and milk samples were collected from New Zealand White WZW) and Californian (Cal) lactating does immediately before mating, at day 14 and day 28 of gestation period The constituents estimated in blood (B) and milk (M) were prolactin, progesterone, total protein, total fat, sugar, calcium (Ca), magnesium Mg), Sodium (Na}, potassium (K), as well as, hemoglobin. Rectal temperature, respiratory and pulse rates were estimated. The results showed that Cal does, generally, exceeded NZW ones significantly (P<O.O1 and 0.05) in all traits studied either in blood or in milk, but Mll3 constituent ratios were inconsistent. All constituents of milk were many folds as much as those for blood, except Na which was about only two &ir& of that of blood in milk, The mineral levels decreased gradually in the following order: Na, K, Ca to Mg in blood and Ca, K, Na to Mg inmilk, respectively, in the two breeds studied. Each of prolactin and progesterone levels increased as milk yield increased and decreased as pregnancy advanced (and milk yield decreased}. Total protein (in B and MJ and blood fat decreased, while milk fat, sugar and Na, generally, increased as pregnancy and suckling advanced The levels of minerals investigated (in B and M) were significantly (FW.01) affected by pregnancy stage, except blood Mg which was not affected Haemoglobin decreased and respiratory rate increased (P<O.Ol} with the advancement of pregnancy stage. Correlation coefficients between each pair of traits studied in blood and milk either within breed or pregnancy stage were mostly not significant and different in sign
To cite th is article / Pou r citer cet article -Addition al en ergy su pplemen ts in th e diet f... more To cite th is article / Pou r citer cet article -Addition al en ergy su pplemen ts in th e diet for fatten in g rabbits.

Live body weight and daily body gain of Nile tilapia fish decreased significantly (P<0.001) with ... more Live body weight and daily body gain of Nile tilapia fish decreased significantly (P<0.001) with increasing dietary lead level. Live body weight and daily body gain of Nile tilapia increased significantly with vitamin E or calcium supplementation in diets. Fish fed diets supplemented with calcium recorded higher body weigh and growth rate than the other experimental groups. Daily feed intake decreased significantly with increasing dietary lead level. Increasing dietary lead level impaired the feed conversion during the whole experimental period. Vitamin E and calcium supplementation in fish diets significantly (P<0.05) increased feed intake and improved feed conversion insignificantly. Within each lead level, daily feed intake slightly increased and feed conversion improved with the Vitamin E or calcium supplementation during the whole experimental period (0 to 4 months). Blood hemoglobin, serum total protein, albumin were significantly (P<0.001) decreased, while urea-N and creatinine, AST and ALT significantly (P<0.001) increased with increasing dietary lead level. Blood hemoglobin, serum total protein, albumin were significantly (P< 0.01 or 0.001) increased, while urea-N, creatinine, AST and ALT significantly (P<0.05 or 0.001) decreased with vitamin E or calcium supplementations in fish diets. Blood hemoglobin, serum total protein, albumin, globulin, creatinine and AST were insignificantly affected by the interaction between lead level and dietary supplementation, while urea-N and ALT levels were significantly (P<0.05) affected. Body moisture and ether extract contents were significantly (P<0.01) decreased, while protein content was significantly (P<0.05) increased by increasing dietary lead level. Body ash did not affected by dietary lead level. Ether extract contents were significantly (P<0.01) increased, while protein contents were significantly (P<0.05) decreased by dietary supplementation. Body composition did not affected by the interaction between lead level and dietary lead level. Body lead residues increased significantly (P<0.001) by increasing lead level in fish diets. Vitamin E and calcium supplementation significantly (P<0.01) decreased lead residues in fish bodies.
To cite th is article / Pou r citer cet article stu dy on u sin g differen t en ergy levels for g... more To cite th is article / Pou r citer cet article stu dy on u sin g differen t en ergy levels for growin g rabbits in

اﻟﻤﻠﺨﺺ اﻟﻜﺮﺑﻮﻓﯿﻮران ﻟﻤﺒﯿﺪ اﻟﻤﻤﯿﺘﺔ اﻟﻐﯿﺮ اﻟﺘﺮﻛﯿﺰات ﺗﺄﺛﯿﺮ ﻋﻠﻰ ﻟﻠﻮﻗﻮف اﻟﺪراﺳﺔ ھ... more اﻟﻤﻠﺨﺺ اﻟﻜﺮﺑﻮﻓﯿﻮران ﻟﻤﺒﯿﺪ اﻟﻤﻤﯿﺘﺔ اﻟﻐﯿﺮ اﻟﺘﺮﻛﯿﺰات ﺗﺄﺛﯿﺮ ﻋﻠﻰ ﻟﻠﻮﻗﻮف اﻟﺪراﺳﺔ ھﺬه أﺟﺮﻳﺖ ) ﻓﯿﻮرازد ( اﻟﻜﺮﺑ ﻣﻦ وھﻮ اﻟﺘﻰ ﻤﺎت ﻓﻰ اﻟﺘﻜﺎﺛﺮى ﺑﺎﻷداء وﺛﯿﻖ ارﺗﺒﺎط ﻟﮫﺎ اﻟﺘﻰ واﻟﺒﯿﻮﻛﻤﯿﺎﺋﯿﺔ اﻟﻔﺴﯿﻮﻟﻮﺟﯿﺔ اﻟﻨﻮاﺣﻰ ﺑﻌﺾ ﻋﻠﻰ اﻟﻨﯿﻤﺎﺗﻮدا ﻟﻤﻘﺎوﻣﻪ ﺗﺴﺘﺨﺪم اﻟﻨﯿﻠﻰ اﻟﺒﻠﻄﻰ إﻧﺎث . ﺧﻼل اﻟﺒﺎﻟﻐﺔ أﺳﻤﺎك ﻟﻨﺼﻒ اﻟﻘﺎﺗﻠﺔ اﻟﺠﺮﻋﺔ ﺗﻌﯿﻦ ﻓﺒﻌﺪ ٩٦ ﻛﺎﻧﺖ أﻧﮫﺎ وﺟﺪ واﻟﺘﻰ ﺳﺎﻋﺔ ٣،٠٤ ﺟﺰء اﺧﺘﺒﺎر ﺗﻢ اﻟﻤﻠﯿﻮن، ﻓﻰ ١ / ٥٠ ، ١ / ٢٥ ، ١ / ١٠ اﻟﺘﻜﺎﺛﺮﻳﺔ دورﺗﮫﺎ أﺛﻨﺎء اﻹﻧﺎث ﻋﻠﻰ ﺗﺄﺛﯿﺮھﺎ ﻟﺪراﺳﺔ ﻣﻨﮫﺎ . أظﮫﺮت وﻟﻘﺪ ﻛﺎﻵﺗﻰ ﻋﻠﯿﮫﺎ اﻟﻤﺘﺤﺼﻞ اﻟﻨﺘﺎﺋﺞ ﻛﺎﻧﺖ و ﻣﺘﺰﻧﺔ ﻏﯿﺮ ﺣﺮﻛﺎت ﻣﻦ ﺗﺄدﻳﻪ ﺑﻤﺎ ﺳﻠﻮﻛﮫﺎ ﻓﻰ اﺿﻄﺮاﺑﺎ ﺑﺎﻟﻤﺒﯿﺪ اﻟﻤﻌﺎﻣﻠﺔ اﻷﺳﻤﺎك : ا اﻟﺪم ﻛﺮات ﻟﻌﺪد ﻣﻌﻨﻮي اﻟﻐﯿﺮ اﻟﻨﻘﺼﺎن ﻓﻰ ﺗﺴﺒﺐ اﻟﻤﺨﺘﻠﻔﺔ ﺑﺘﺮﻛﯿﺰاﺗﻪ اﻟﻤﺒﯿﺪ ﻟﺤﻤﺮاء ) RBCs ( اﻟﮫﯿﻤﻮﺟﻠﻮﺑﯿﻦ وﻣﺤﺘﻮى ) Hb ( اﻟﺪم ﻓﻰ اﻟﮫﯿﻤﺎﺗﻮﻛﺮﻳﺖ وﻧﺴﺐ ) Hct ( اﻟﺤﻤﺮاء اﻟﺪم ﻛﺮات ﺣﺠﻢ وﻣﺘﻮﺳﻄﺎت )) MCV ﺑﮫﺎ اﻟﮫﯿﻤﻮﺟﻠﻮﺑﯿﻦ وﻛﻢ ) MCH ( اﻟﺪم ﻓﻰ اﻟﮫﯿﻤﻮﺟﻠﻮﺑﯿﻦ ﺗﺮﻛﯿﺰ ﻛﺬا ) MCHC ( اﻟﺪم ﻟﻮن ﻣﻌﺎﻣﻞ إﻟﻰ ﺑﺎﻹﺿﺎﻓﺔ ) CI .( اﻟﻤﺨﺘﻠﻔﺔ ﺑﺘﺮﻛﯿﺰاﺗﻪ اﻟﻤﺒﯿﺪ أن ﻛﻤﺎ اﺣ ﻧﻘﺼﺎن ﻓﻰ ﺗﺴﺒﺐ ﻛﺬا و ﺗﺄﺛﯿﺮه ﺗﺤﺖ اﻟﻮاﻗﻌﺔ اﻷﺳﻤﺎك دم ﻓﻰ اﻟﻜﻠﯿﺔ واﻟﺒﺮوﺗﯿﻨﺎت اﻟﺪھﻮن ﻓﻰ اﻟﻤﻌﻨﻮﻳﺔ ﻣﺘﺒﺎﻳﻦ ﺼﺎﺋﻰ اﻟﻤﺒﯿﺪ ﺗﺮﻛﯿﺰ ﺑﺰﻳﺎدة ﻳﺰداد اﻟﻨﻘﺼﺎن ھﺬا وﻛﺎن اﻹﻧﺎث ﻓﻰ اﻻﺳﺘﺮادﻳﻮل ھﺮﻣﻮن . واﻟﯿﻮرﻳﺎ ﻟﻠﺠﻠﯿﻜﻮز ﺣﺪث ﻣﺎ ﺑﺨﻼف ھﺬا اﻷﻣﯿﻦ ﻟﻤﺠﻤﻮﻋﺔ اﻟﻨﺎﻗﻠﺔ اﻹﻧﺰﻳﻤﺎت ﻛﺬا اﻟﺪم ﻓﻰ واﻟﻜﺮﻳﺎﺗﯿﻦ ) AST , ALT ( ﻧﻔﺲ ﻓﻰ ﻋﺎﻟﯿﺔ ﺑﻤﻌﻨﻮﻳﺔ زﻳﺎدﺗﮫﺎ ﻟﻮﺣﻆ واﻟﺘﻰ اﻟﻀﺎﺑﻄﺔ اﻟﻤﺠﻤﻮﻋﺔ أﻓﺮاد ﻣﻊ ﻣﻘﺎرﻧﺘﺎ اﻷﺳﻤﺎك ھﺬه . اﻷﺳﻤﺎك وﻣﻨﺎﺳﻞ ﻛﺒﺪ ﻓﻰ ﻛﺒﯿﺮ ﺿﺮر إﺣﺪاث إﻟﻰ أدى ﺟﻤﯿﻌﻪ وھﺬا ﻛ ﻣﻤﺎ واﻟﻤﻨﺎﺳﻞ اﻟﻜﺒﺪ ﻣﻌﺎﻣﻞ ﻧﻘﺼﺎن ﻋﻠﻰ اﻟﺒﺎﻟﻎ اﻷﺛﺮ ﻟﻪ ﺎن ) اﻟﻤﺒﺎﻳﺾ ( إﻧﺎث ﻋﻨﺪ ﻓﻰ واﻟﻨﺴﺒﯿﺔ اﻟﻤﻄﻠﻖ اﻟﺨﺼﻮﺑﺔ وﻛﺬا اﻟﻨﯿﻠﻰ اﻟﺒﻠﻄﻰ . Abstract

The present study was carried out to evaluate the effects of probiotics on growth performance, fe... more The present study was carried out to evaluate the effects of probiotics on growth performance, feed utilization and survival rate of Nile tilapia (Oreochromis niloticus) during the period from June to September 2011. A total number of 120 fish was randomly divided into four main groups (each containing 30 fish); the 1st group was fed on a diet without any supplementation bacterial, the 2nd , 3rd and 4th groups were fed on a diet supplemented with Lactobacillus acidophilus or Streptococcus thermophilus or Bifidobacterium bifiduim (1 ml/100 g diet), respectively. The obtained results could be summarized as follows: Fish group which fed on a diet supplemented with Lactobacillus acidophilus recorded significantly (P<0.05 and 0.01) the highest live body weight at most of the experimental periods when compared with the other fish groups. In general, the diet supplemented with Lactobacillus acidophilus achieved the best values for daily weight gain and specific growth rate followed by B...

Forty two New Zealand White (NZW) male rabbits with average body weight 685.4±11.7 g were randoml... more Forty two New Zealand White (NZW) male rabbits with average body weight 685.4±11.7 g were randomly assigned to 6 groups (7 animals in each) in a 3 x 2 factorial design to evaluate the ability of coumarin on alleviation the toxicity of aflatoxin B 1 . Rabbits of the first 3 groups were given basal diet (contained 17% yellow corn, 35% clover hay, 10% barley, 13% soybean meal, 20% wheat bran, 3.0% molasses, 0.20% sodium chloride, 0.40% vitamins and minerals, 1.0% premix and 0.40% limestone) without aflatoxin B 1 , while the other 3 groups were given the basal diet containing 0.25 ppm aflatoxin B 1 . Within each the previous two groups they were divided to other 3 subgroups: the first group fed diet without any treatment (control), the second and third groups were supplemented with 2.5 and 5 g coumarin/kg diet, respectively. Feeding rabbits contaminated diet with aflatoxin B 1 were significantly reduced daily feed intake, feed conversion, and digestibility of crude protein. Also, red blood cells count (RBCs) as well as hemoglobin, total protein and albumin concentrations were decreased in blood rabbits feed with contaminated diet. This in turn was reflected as a reduction in total and daily body weight gain and an increase in mortality rate. Adding coumarin at rate of 2.5 or 5g / kg diet contaminated with aflatoxin B 1 were significantly (P<0.05) reduced the negative effects of aflatoxin B 1 on total body weight and daily weight gain, feed intake, feed conversion, digestibility of nutrients (organic matter, crude protein, crude fiber, ether extract and nitrogen free extract), blood parameters (hematokrite %, red blood cells count, total protein and albumin concentration, aspertate aminotransferase and alanine aminotransferase activities), mortality rate, residue of aflatoxin B 1 in rabbit thigh muscles and economical efficiency. There was no significant difference between the two tested coumarin levels. In conclusion, adding coumarin (2.5 g coumarin/ Kg diet) to aflatoxin B 1 contaminated rabbit diet was safe and practical method to minimize aflatoxin B 1 toxicity.

he aim of this study was to investigate the toxic effects of aflatoxin B 1 on Nile tilapia Oreoch... more he aim of this study was to investigate the toxic effects of aflatoxin B 1 on Nile tilapia Oreochromis niloticus and attempt to detoxify these drastic effects by using ozone treatment and natural clay (bentonite) and coumarin supplementation in diets. Three hundred and sixty healthy fingerlings of Nile tilapia (7.3 g) were randomly distributed into 24 glass aquaria, representing 8 treatments. Fish of the first 4 groups were given basal diet without aflatoxin B 1 , while the other 4 groups were given basal diets containing 0.025 ppm aflatoxin B 1 . Within each the aflatoxin treatment, the first group (control group) fed the basal diet without any treatment, the second group was fed the basal diet but the water of aquarium treated with ozone (0.5 mg/L/minute), the third group was fed the basal diet supplemented with 5 g coumarin/kg diet and the fourth group fed the basal diet supplemented with 20 g clay/kg diet. The interaction between aflatoxin and treatments insignificantly affected...

The present study was conducted out at the department of Animal Production, Faculty of Agricultur... more The present study was conducted out at the department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, Egypt. The practical work was carried out at sheep farm belongs to Animal Nutrition Research unit, Radiobiology Applications Department, Nuclear Research Center, Atomic energy Authority, Abu Zabeel, Qaluobia Governorate from August to November 2009, to investigate the effect of chemical and biological treatment of rice straw on performance of male lambs, nutrient digestibility and some blood chemical constituents. Twenty-eight Barki male lambs with average initial live body weight (LBW) of 31.75 kg and about five month of age were divided into four similar groups in live body weight and age (eight animals each). The first group (T1) was fed basal diets which composed of concentrate feed mixture (CFM) and untreated rice straw (RS), while the second group (T2) was fed basal diets which composed of concentrate feed mixture (CFM) and berseem hay (BH). The anim...

A total number of 240 laying hens and 24 cocks of Silver Montazah strain at 40 weeks of age were ... more A total number of 240 laying hens and 24 cocks of Silver Montazah strain at 40 weeks of age were randomly divided into 12 groups (20 hens and 2 cocks / each treatment group). Birds in all treatments were nearly similar in the average initial body weights and average daily egg production A 4 x 3 factorial design experiment was performed including four levels of supplemented lead (0. 250,500 and 1000 mg/kg diet) and three levels of feed additives (without clay or vitamin E, 3 % Natural clay, tafla, and 200 mg /kg vitamin E). Live body weight change, egg production and egg weight, egg compenents, fertility and hatchability were studied. Results obtained reveled that lead contaminated feed caused significant live body weight retardation, lower egg production and egg weight, lower egg component and lower fertility and hatchability. On the other hand, the addition of natural clay or vitamin E to the diets for laying hens caused a significant improve in all aforementioned traits. With resp...

The present study was carried out to investigate the influences of sublethal toxicity of Isoproth... more The present study was carried out to investigate the influences of sublethal toxicity of Isoprothiolan ( Fuji-one), which is one of the most commonly used fungicide, on the biochemical parameters and reproductive performance of female Oreochromis niloticus. The 96 hr LC50 of the Fuji-one was determined for the adult Nile tilapia and it was 6.05 μg/ml. The present experimental assay was evaluated on the 60th day of exposure to 1/12 1/6 and 1/3 LC50 of the pesticide in comparison with control fish group. The induced hazard effects were obvious in blood parameters as well as blood chemistry. The insignificant decrease of the erythrocytes count (RBCs), haemoglobin content (Hb) and hematocrit value (Hct) were dose dependent. The plasma glucose level of the treated fish was significantly increased (hyperglycemia) in dose dependent response to fuji-one. Nonetheless, the total plasma lipid was significantly high and total plasma protein was significantly lower under the effect of intoxicati...

The present study aimed to sex reverse some genotypic female O. niloticus into phenotypic males a... more The present study aimed to sex reverse some genotypic female O. niloticus into phenotypic males after hatching using different water temperatures (28°C the Control, 32°C, 34°C and 36°C), the aquaria were supplied with air pump and with a thermostat to adjust the water temperature, the experimental period was 30 days post hatching (W=0.008g/fish, L=0.6 cm/fish), Total number was 1200 fish (100 fish per aquaria) and fish were fed on the basal diet 40% crude protein at rate of 20% of the body weight and 12% from body weight for another 50 days at 28 o C and investigate final weight, male % and survival rate of fish indoor aquarium. Highly significant increase (P<0.0001) in final body weight, body length, and weight gain after 30 and 80 days was recorded in control as compared with other treatments 32, 34 and 36°C While, body weight of fry exposed to 36°C, decreased significantly after both 30 and 80 days when compared with fry in the control group (28°C) reared in glass aquariums. T...
The objective of the present study was to evaluate the possibility of replacing a great portion p... more The objective of the present study was to evaluate the possibility of replacing a great portion part of fish-meal in the diet of Nile tilapia fish with different level of dietary soybean-meal. Tilapia Oreochromis niloticus individuals weighing approximately 1.5 g of the stock raised in Abbassa fish hatchery, Egypt were randomly distributed in 18 nylon haps representing 6 dietary treatments. The first three treatments were fed on diets contained 25% crude protein, while the other three treatments were fed on diets contained 30% crude protein. Within each dietary protein level, the fish in experimental groups were fed diets formulated to replace the 50%, 75% and 100% of fish-meal protein with soybean-meal protein.
Journal of Applied Aquaculture, 2014
The aim of this study was to evaluate Lactobacillus acidophilus, Streptococcus thermophilus, Bifi... more The aim of this study was to evaluate Lactobacillus acidophilus, Streptococcus thermophilus, Bifidobacterium bifidum, and yeast Saccharomyces cerevisiae singly and in combination as probiotic supplements in Nile tilapia (Oreochromis niloticus) fingerling diets. All the probiotic-supplemented diets resulted in growth higher than the control. Best growth rate, food consumption, and food conversion (P < 0.05) were in the group fed a cocktail of the three bacteria. Feed cost, return on weight gain, and profit margin increased in fish groups fed diets inoculated with probiotics. Supplementation with Lactobacillus acidophilus, Bifidobacterium bifiduim, and the three-bacteria cocktail were most effective in eliminating mortality in an A. hydrophila challenge.
SUMMARY: A total of 32 males wead New Zealand W5ite (MW) rabbits (7 weeks of age) were divided in... more SUMMARY: A total of 32 males wead New Zealand W5ite (MW) rabbits (7 weeks of age) were divided inIo 4 groups (8 each) to be used in a study aimed to utilize the animal and vegetable fats (tallow and palm oil) and starch in pelleted diets as additional energy source in feeding growing rabbits compared with the basal diet (without
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Papers by Prof. Dr. Mohamed Salah Ayyat