Papers by Mahmoud Mohamed Ibrahim Alagwany
Journal of Animal and Feed Sciences, 2015
(Egypt). All of the experimental procedures were carried out according to the Local Experimental ... more (Egypt). All of the experimental procedures were carried out according to the Local Experimental Animals Care Committee, and approved by the institutional ethics committee. The birds were cared for using husbandry guidelines derived from Zagazig University standard operating procedures.
Milad Adel (2016) Beneficial impacts of thymol essential oil on health and production of animals,... more Milad Adel (2016) Beneficial impacts of thymol essential oil on health and production of animals, fish and poultry: a review,
Having accurate data for ileal amino acid digestibility of a feed ingredient is one of the ways t... more Having accurate data for ileal amino acid digestibility of a feed ingredient is one of the ways to enhance protein and nitrogen utilisation, improve poultry feeding efficiency, and mitigate ammonia emission and pollution in the environment. Moreover, the precise estimation of endogenous amino acid losses (EAAL) depends on formulating diets on digestible amino acid (DAA) basis. Numerous methods have been reported to determine the endogenous fractions including the regression method, the protein-free diet, and the total digestible nitrogen diet. The EAAL determined by these techniques can help in calculated corrected values for amino acid digestibility, termed true digestibility. Certain dietary components that can be called 'specific losses' could lead to higher losses than the basal calculation would indicate. To determine the basal plus specific losses (total endogenous losses), some researchers suggested the 15 N-dilution method, which allows the determination of the actual digestibility. This method needs more studies and evaluations because there are some controversial issues about this methodology, such as the validity of the 15 N-dilution technique when applied to any amino acid or to total nitrogen, the reference pool for the estimation of the labelling of endogenous fractions/secretions, anti-nutritional agents, the effect of bird age, mucin, different cereal grains, as well as the marker itself and the proper assessment. This review will be focused on the above issues, finding that the recently reported 15 N isotopes single injection method could be an easy, time-saving, consistent, and reliable methodology for EAAL estimation in poultry.
A trial was conducted to evaluate the effect of different processing methods of pigeon pea (Pp; C... more A trial was conducted to evaluate the effect of different processing methods of pigeon pea (Pp; Cajanus cajan) on fattening performance and carcass traits as well as blood biochemical and hematological parameters of broiler chickens. For this purpose, 300 dayold chicks were assigned to 5 treatments. Each treatment had 5 replicates and contained 12 birds each under a completely randomized design. Five isonitrogenous and isocaloric diets were formulated with the inclusion of pigeon pea crushed (control, PPC), boiled with potash (PPP), boiled (PPB), soaked (PPS), or roasted (PPR) at the rate of 20% of the basal diet. The highest values of weight gain were observed in chicks fed the PPP diet through the starter and overall periods. Throughout the starter phase, the feed conversion ratio was significantly affected by dietary treatments. Highest values for carcass and breast weight were observed in birds fed the PPP diet as compared to the control diet. Blood biochemical parameters were not statistically influenced by Pp treatments. Hematology was also similar in all the diets. In conclusion, PPP can be used as a protein source in broiler diets and it can improve the growth performance of broilers.
This study evaluated the effect of thyme (Thymus vulgaris L.) on productive performance, egg qual... more This study evaluated the effect of thyme (Thymus vulgaris L.) on productive performance, egg quality, blood profile, immune function and antioxidant enzyme activity in laying hens. A total of 96 thirty-six-week-old laying hens were assigned to a basal control diet or a diet supplemented with 3, 6, or 9 g · kg -1 of thyme fed from 36 to 52 weeks of age. Hens were divided into four equal groups replicated six times with four hens per replicate. No significant differences were observed among the dietary treatments in body weight change, feed consumption, or feed conversion ratio. Supplementation of thyme up to 6 g · kg -1 in the diet led to an increase in egg weight compared with the basal diet. Egg number was not (P > 0.05) influenced by thyme supplementation, except from 36 to 40 weeks of age. Birds fed diets with 3 or 6 g · kg -1 of thyme had greater (P < 0.05) egg mass than those fed the unsupplemented diet or that with 9 g · kg -1 of thyme. Dietary supplementation with thyme exhibited a significantly positive effect on parameters related to immunity and lipid profile. Serum superoxide dismutase activity and reduced glutathione concentration were significantly increased in groups fed diets with thyme (P = 0.010 and P < 0.001, respectively). The malondialdehyde concentration was decreased (P = 0.048) in experimental groups in comparison with the control. In conclusion, thyme supplemented up to 9 g · kg -1 diet can be used as an effective feed additive to improve productive performance, blood profile and antioxidant enzyme activities in laying hens.
This study was carried out to evaluate the effects of different concentrations of cyadox (CYA), c... more This study was carried out to evaluate the effects of different concentrations of cyadox (CYA), cinnamaldehyde (CIN) and resveratrol (RES) on rabbit isolated erythrocytes in two experiments. Experiment 1 evaluated the effects of different concentrations (2.5, 5, 10, 20, 40, 60, 80 and 100) μg/ml of CYA and μM of both CIN and RES separately on isolated erythrocytes in order to make the concentration response curve. Results indicated that CYA caused a significant depletion of reduced glutathione (GSH) content compared to control at 40 μg/ml and the depletion increased by increasing the concentration. On the other hand, both RES and CIN showed a highly significant elevation of GSH content at 40 μM being more effective by increasing concentrations. Experiment 2 investigated the potential benefits of using CIN and RES separately or in combination on CYA induced alterations in isolated rabbit erythrocytes (the used concentrations were based on the results of experiment 1). Results revealed that exposure to CYA caused a significant decrease in superoxide dismutase (SOD) and catalase (CAT) activities and reduced glutathione (GSH) and total protein (TP) contents, CYA also elevated extracellular hemoglobin (Hb) and adenosine triphosphate (ATP), increased the malonaldehyde (MDA) and protein carbonyl (PrC) contents with increasing caspase3 and caspase8 activities suggesting CYA pro-oxidant effect. Both CIN and RES were able to inverse these hazardous effects of CYA. However, CIN was more effective than RES, and their combination showed a positive synergistic effect in protecting cells against oxidative injury caused by CYA.
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
The main objective of this work was to investigate the impact of dietary chromium supplementation... more The main objective of this work was to investigate the impact of dietary chromium supplementation on growth indices, carcass yield, and some hematological and biochemical blood parameters of growing Japanese quails subjected to heat stress. A total of 360 unsexed 2-week-old Japanese quail chicks were used in a 2 × 3 factorial arrangement that had two ambient temperatures (23 ± 2 and 33 ± 2 °C) and three dietary chromium (0.00, 500, and 1000 μg Cr/kg diet as chromium picolinate). For induction of heat stress, the room temperature was set at 33 ± 2 °C from 2 to 6 weeks of age. Results showed that body weight, body weight gain, and feed intake were decreased for birds subjected to heat stress condition during 2 to 4 and 2 to 6 weeks of age. Feed conversion was not significantly (P > 0.05) affected by high ambient temperature throughout the experiment. Carcass, dressing, liver, and heart percentages were not influenced by the ambient temperature or dietary chromium or their combinations. Significant temperature × chromium combinations were observed for hemo-globin value (P = 0.025) and packed cell volume (P = 0.001). Cholesterol and glucose in plasma were increased (P = 0.004 or 0.022) in quails subjected to heat stress condition. Plasma measurements of total proteins, albumin, globulin, lipids, glucose , and A/G ratio of quail chicks were not influenced (P > 0.05) by chromium, while cholesterol was increased with increasing chromium level (P = 0.033). High ambient temperature or dietary chromium levels or their interactions did not (P > 0.05) affect plasma levels of aspartate transaminase (AST), alanine transaminase (ALT), triiodothyronine (T3), thyroxin (T4), or T3/T4 ratio of growing quails. From these observations, it can be concluded that dietary chromium sup-plementation of growing Japanese quail subjected to heat stress condition could beneficially affect growth performance and carcasses, as well as modulate the hematological and biochemical blood parameters, probably through modulating stress status.
A study was conducted using 162 Bovans laying hens to investigate the impacts of extra dietary vi... more A study was conducted using 162 Bovans laying hens to investigate the impacts of extra dietary vitamin A (0, 8000, 16,000 IU/kg), selenium (0, 0.25, 0.50 mg/kg), and their combinations on the performance, egg quality, and blood biological parameters of laying hens during summer months. Supplemental vitamin A up to 16,000 IU/kg diet significantly (P < 0.05) improved all productive traits studied except feed intake which increased with 8000 IU/kg diet compared with control. Feed intake and feed conversion of hens fed diet supplemented with selenium revealed high statistical (P = 0.001) differences. All egg quality criteria were not significantly (P < 0.05) affected by dietary vitamin A except albumin percentage and Haugh units, since Haugh unit score was gradually increased with increasing vitamin A level. Vitamin A-enriched groups showed significant (P < 0.05 and 0.01) decreases in plasma albumen, total lipids, and total cholesterol in respect to the unsupplemented groups. Compared with unsupplemented groups, total protein, albumin, total lipid, and total cholesterol were increased in selenium-enriched groups. There were no significant impacts of selenium treatments in layer diets on thyroid hormones and liver enzymes studied except alanine transferase (ALT) and thyroxin (T 4), as compared with the control group. Hematological parameters were not affected by vitamin A treatment except PCV% which decreased with vitamin A supplementation. Hemoglobin and lymphocytes were increased with increasing dietary selenium level. In conclusion, the combined supplementary concentrations of vitamin A (16,000 IU/kg) and selenium (0.25 mg/kg) might be needed for better production and health of laying hens reared under heat stress conditions.
A total of 120 Japanese quails were randomly distributed into six dietary groups to evaluate the ... more A total of 120 Japanese quails were randomly distributed into six dietary groups to evaluate the effect of low-crude protein (CP) diet supplemented with crystalline amino acids on productive and reproductive performance, blood parameters and nitrogen (N) balance. Treatments consisted of six different diets: a control diet (20% CP), a low-CP (16%) diet supplemented with threonine (Thr), valine (Val) and tryptophan (Trp), three low-CP diets supplemented with individual Thr, Val or Trp, and a low-CP diet supplemented with all aforementioned amino acids. None of the growth traits examined were influenced either by dietary treatments or by egg production (P > 0.05). The highest fertility percentages (88.2%) were observed in quails fed the low-CP diet without crystalline amino acids supplementation followed by low-CP diet supplemented with Val (86.2%), whereas the highest hatchability (from fertile eggs) percentage was observed in quails fed low-CP diet plus Trp. Egg shell percentages from quails fed the control diet were higher (P < 0.05) compared to other dietary treatments during the whole feeding period. Yolk percentage was decreased (P < 0.01) with the low-CP diet supplemented with amino acids. Blood protein and ammonia levels were decreased (P < 0.05) by reducing the dietary CP level or by supplementing amino acids to the low-CP diet. Reducing the CP level in the diet with amino acids supplementation in laying quails decreased the daily N excretion (P < 0.05). It is concluded that reducing the CP level in a diet supplemented with crystalline amino acids supports reproductive performance in quails, and is a valuable strategy for decreasing feeding cost and mitigating ammonia emission.
The major goals of this study were to determine the impact of sugar beet pulp (SBP) levels and Av... more The major goals of this study were to determine the impact of sugar beet pulp (SBP) levels and Avizyme ® 1500 (xylanase, protease and amylase) enzyme addition on productive and reproductive performance as well as egg quality, apparent digestibility of nutrients and nitrogen (N) balance in laying Japanese quail between 12 and 20 weeks of age. The experimental design consisted of a 3 × 3 factorial arrangement with three levels of SBP (0, 20 and 40 g kg –1 diet) and three concentrations of Avizyme (0, 1 and 2 g kg –1 diet). There were no differences in feed consumption, feed conversion ratio, egg number, egg weight nor egg mass caused by the treatment. Final body weight was significantly (P ≤ 0.01) decreased by increasing SBP levels. Increasing SBP level from 20 to 40 g kg –1 in the diet of laying quails led to significant reduction in fertility percentage by 2.67 and 7.01 and 4.60% during periods 12–16, 16–20 and 12–20 weeks of age, respectively. Hatchability percentages (from fertile eggs) elevated gradually with decreasing SBP levels in the diets during the whole period. Different levels of SBP and Avizyme, or their interaction, insignificantly affected the external and internal egg quality of the laying quails during the overall experimental period (12–20 weeks of age). Meanwhile, the different levels of SBP had a significant effect on all the digestion coefficients of the nutrients except for the N digestibility. The N consumption (g d –1), N in egg (g d –1), N excretion (g d –1), N faecal, N intake and N retention were not significantly affected neither by SBP inclusion nor by Avizyme supplementation. The overall results indicated that inclusion of SBP and Avizyme in quail diets did not affect productive performance, egg quality criteria and nitrogen balance but reproductive parameters and digestibility of the nutrient statistically decreased with increasing SBP up to 40 g kg –1 diet throughout the overall period (12–20 weeks of age).
| Identification of animal species of origin in meat and meat products is a matter of great conce... more | Identification of animal species of origin in meat and meat products is a matter of great concerns such as religious, economical, legal as well as medical aspects. Thus, several analytical techniques have been suggested for the identification of meat species either in individual or in mixed samples to protect consumers from the fraudulent and bad habits of marketing. DNA-based techniques especially the techniques based on polymerase chain reaction (PCR) are recognized as the most appropriate methods employed for species identification in raw and processed meat. PCR techniques including randomly amplified polymorphic DNA (PCR-RAPD), restriction fragment length polymorphism (PCR-RFLP), PCR with species-specific primers, real-time PCR and PCR-nucleotide sequencing allow identification of meat species under different processing conditions. But the variability of DNA content on the level of species as well as target tissue make the DNA-based methods somewhat unsuitable for the quantification of exact percentages of different species in meat and meat products. For these reasons the proteomic approaches depending on identification of different peptide biomarkers has been developed and employed to give information on the different composition of food. To broad the knowledge about these technologies, this review is compiled in an attempt to provide an overview of the possible PCR-based analytical techniques that could help in identifying the meat species of origin in meat and meat products and threw the light on the identification of species specific peptide biomarkers by proteomic technologies as a new and attractive alternative that could overcome some of the limitations that faced DNAbased methods especially when used for meat exposed to intensive heating of processing as well as for meat mixtures.
The present study aimed to investigate the effect of the dietary levels of protein, methionine (M... more The present study aimed to investigate the effect of the dietary levels of protein, methionine (Met), as well as probiotic on productive performance, feed utilization, and environmental pollution by N in Lohmann Brown laying hens. A total number of 160 Lohmann Brown laying hens at 20 weeks of age were randomly divided into 8 treatment groups using a 2 × 2 × 2 factorial design experiment. The experiment involved two levels of crude protein (16 and 18 %), two levels of Met (0.45 and 0.50 %), and two concentrations of probiotic (0 or 1 g/kg diet, with a concentration of 10 10 CFU/g of Lactobacillus acidophilus) within 20-42 weeks of age. Results revealed that egg production parameters were significantly (P < 0.01) improved for hens fed diets of 18 % CP comparing with that of 16 % protein within the period from 26 to 30 weeks of age. Protein utilization and feed efficiency values were enhanced with 0.67 and 0.72 % Met during the period of 26-30 weeks of the age. For the N pollution, results showed that increasing crude protein in the diet from 16 to 18 % caused significant (P < 0.01) increase in the excreted N from 0.349 to 0.492 g/d. The methionine level of 0.72 % recorded the highest values of total consumed N being 3.98 g/d and excreted N being 0.527 g/d comparing with the other levels. It could be concluded that the best productive performance could be given by using 0.72 % total sulfur amino acids (TSAA). Furthermore, the dietary level of 18 % CP with 0.72 % Met is preferred in feeding laying hens through the whole experimental period. Ecologically, reducing the level of crude protein in layer diets to be 16 % along with the supplementation of Met can play an important role in minimizing the pollution with N from poultry excretion.
This study evaluated the effect of Yucca schidigera extract on productive performance, egg qualit... more This study evaluated the effect of Yucca schidigera extract on productive performance, egg quality, blood metabolites, immune function, and antioxidant parameters in laying hens. A total of 96 36-week-old hens were allocated into four groups, the control diet or the diet supplemented with 50, 100, or 150 mg/kg of yucca extract, from 36 to 52 weeks of age. Hens were divided into four equal groups replicated six times with four hens per replicate. As a result of this study, there were no linearly or quadratically differences in body weight change (BWC), feed consumption (FC), feed conversion ratio (FCR), and egg weight (EW) due to yucca treatments at different ages, except FCR and EW that were improved with yucca supplementation during 36-40 weeks of age. Supplemental dietary yucca up to 100 mg/kg diet led to significant improvement in egg number (EN) and egg mass (EM). Egg qualities were not linearly or quadratically affected by yucca treatments except shell thickness was quadratically (P < 0.001) increased with increasing yucca level up to 100 mg/kg diet. Dietary supplementation of yucca exhibited a positive impact on albumin and immunoglobulin G (IgG). Comparing to the control group, yucca addition to laying hen diets resulted in a significant linear (P<0.001) and quadratic (P=0. 010) decrease in blood ammonia-N and urea-N, respectively. The activity of superoxide dismutase (SOD) and reduced glutathione (GSH) level in serum were quadratically improved in yucca groups. The malondialdehyde (MDA) concentration was decreased with yucca addition in comparison with the control group. In conclusion, yucca supplemented up to 100 mg/kg diet can be used as effective feed additive to improve productive performance, blood profile, and antioxidant enzyme activities in laying hens.
Distiller's Dried Grains with Solubles (DDGS) as untraditional feedstuff has been available for f... more Distiller's Dried Grains with Solubles (DDGS) as untraditional feedstuff has been available for feed industry for many years and became a nutritional and economical feed ingredient. According to recent studies, DDGS is usually used at low concentrations (10 or 15%) as a feed ingredient in laying hen diets without adverse effects on laying performance, feed utilization, nutrient digestibility, egg quality criteria and economics. Because DDGS became available in feed market in large amounts, the ability of using higher DDGS inclusion rate in poultry feed has become an interest for several researchers and poultry producers. Using such untraditional feedstuffs aims mainly to decrease the feed costs. Researchers assured that feeding higher levels of DDGS could have a significant effect on the cost of feed for poultry producers because of higher availability of DDGS and the current price fluctuations of feed ingredients. On the other hand, beneficial effects of DDGS on the environment have also been reported. These beneficial effects could be represented through mitigating harmful emissions and pollution from manure which provides production within clean atmosphere. Findings from different researches on the use of DDGS as feed ingredient for laying hens are illustrated in this review. Moreover, this study describes advanced information regarding the use of corn DDGS as untraditional feedstuff for laying hens involving its production process and nutritive value along with its useful effects on laying hens production traits, egg quality, apparent digestibility, economics and the impact on the surrounding environment in poultry house.
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Papers by Mahmoud Mohamed Ibrahim Alagwany