Scientific Articles by Gabriela Boza-Moran
Hamilton, 2024
In this Case Study, the EU-OPENSCREEN compound storage and management facility in Berlin, Germany... more In this Case Study, the EU-OPENSCREEN compound storage and management facility in Berlin, Germany, describes how it used a highly integrated automated system to produce over 1.5 million compound aliquots of its Compound Collection for High-Throughput Screening (HTS) studies at its partner sites.
Highlights of this Case Study:
- Description of a highly integrated system consisting of thirteen instruments, including a Hamilton Verso® Compound Management System and two Hamilton Microlab® STAR(let) liquid handlers
- Detailed description of the automated methods for compound dissolution and aliquoting, compound (un)loading to/from -20 °C storage, and compound reformatting
- Data exchange among instruments and use of the Hamilton VENUS® software as the central hub
Link to free Case Study: https://hubs.li/Q02Z_DMg0
Hamilton, 2024
In this Case Study, InSphero, a Swiss-based company specializing in 3D in vitro cell models and c... more In this Case Study, InSphero, a Swiss-based company specializing in 3D in vitro cell models and cell culture tools for drug discovery, describes the automated production and maintenance of spheroids via self-aggregation using their proprietary Akura™ technology and Hamilton’s Microlab® STARlet. Link to full Case Study: https://hubs.li/Q02t7hHz0
Hamilton, 2023
In this Case Study, the Genomics Center of Excellence (CoE) at Al Jalila Children’s Specialty Hos... more In this Case Study, the Genomics Center of Excellence (CoE) at Al Jalila Children’s Specialty Hospital in Dubai describes the automation of an Illumina-based rapid Whole-Genome Sequencing (rWGS) workflow, aiming to provide quicker diagnoses for neonatal patients. Link to free Case Study: https://hubs.li/Q026y-VR0
Hamilton Robotics, 2023
In this Case Study, the Analytical Development group at AGC Biologics Milan, describes the automa... more In this Case Study, the Analytical Development group at AGC Biologics Milan, describes the automation of two ELISA-based workflows to assess lentiviral vector purity, using a Hamilton Microlab® STARlet liquid handler integrated with a plate washer, a plate spectrometer, and two plate incubators.
Highlights of this Case Study
- Overview of the analytical methods automated by AGC Biologics Milan on Hamilton liquid Handlers
- Detailed description of two ELISA-based automated workflows to assess the purity of the lentiviral vectors: residual benzonase and residual Host-Cell Proteins (HCP)
- Advantages of the Hamilton VENUS Scheduler to plan and execute parallel assays using the same sample aliquot
Link to Case Study: https://hubs.li/Q01CV6MV0
Hamilton, 2023
In this Case Study, Oxford Biomedica, an innovative global gene and cell therapy company with ope... more In this Case Study, Oxford Biomedica, an innovative global gene and cell therapy company with operations in the UK and US, describes the automation of a 10-day cell-based workflow required for an analytical test to determine viral vector titer, using Hamilton Microlab® STARTM liquid handlers.
Highlights of this Case Study
- Detailed description of the automated system used for the cell-based workflow
- Advantages of Hamilton's CECULA Graphical User Interface (GUI) for the planning of long-term cell-based assays
- Transfer of the automated assay to Good Manufacturing Practice (GMP)
Link to free Case Study: https://hubs.li/Q01xk41G0
Hamilton, 2022
In this Case Study, the National Health Laboratory Service (NHLS) Laboratory at Tambo Memorial Ho... more In this Case Study, the National Health Laboratory Service (NHLS) Laboratory at Tambo Memorial Hospital in Boksburg, South Africa, describes how they set up a flexible, easy-to-use, high-throughput automated workflow for qPCR-based SARS-CoV-2 testing, using Hamilton (Assay-Ready) Workstations.
Highlights of this Case Study:
· NHLS COVID-19 Testing Strategy in South Africa
· Description of the automated SARS-COV-2 Testing and its benefits
· Beyond Testing: SARS-COV-2 surveillance via NGS sequencing
Link to free Case Study: https://hubs.li/Q011-ySB0
Hamilton , 2021
In this Case Study, we describe the complete workflow of the newly automated method for the isol... more In this Case Study, we describe the complete workflow of the newly automated method for the isolation of mononuclear cells from blood and bone marrow samples, developed at BioBank Dresden.
Highlights of this Case Study:
The role of BioBank Dresden in Cancer Research
From manual to automated sample processing: challenges and benefits
Detailed system and workflow description
Link to free Case Study: https://hubs.li/H0_5RPv0
The American Journal of Human Genetics , 2021
Recent studies indicate that neurodegenerative processes that appear during childhood and adolesc... more Recent studies indicate that neurodegenerative processes that appear during childhood and adolescence in individuals with Wolfram syndrome (WS) occur in addition to early brain development alteration, which is clinically silent. Underlying pathological mechanisms are still unknown. We have used induced pluripotent stem cell-derived neural cells from individuals affected by WS in order to reveal their phenotypic and molecular correlates. We have observed that a subpopulation of Wolfram neurons displayed aberrant neurite outgrowth associated with altered expression of axon guidance genes. Selective inhibition of the ATF6α arm of the unfolded protein response prevented the altered phenotype, although acute endoplasmic reticulum stress response—which is activated in late Wolfram degenerative processes—was not detected. Among the drugs currently tried in individuals with WS, valproic acid was the one that prevented the pathological phenotypes. These results suggest that early defects in axon guidance may contribute to the loss of neurons in individuals with WS.
Hamilton , 2021
In this Case Study, the Toxicology Laboratory of Labor Berlin, a German company from Charité and ... more In this Case Study, the Toxicology Laboratory of Labor Berlin, a German company from Charité and Vivantes, explains the benefits of automating the sample prep workflow for Drugs of Abuse (DoA) testing in oral fluid samples.
Highlights of this case study:
• Benefits of using oral fluid samples as a matrix for LC-MS analysis
• Description of a new LC-MS sample prep method for DoA testing in oral fluid samples
• Benefits of developing this new testing method using automation
Link to free Case Study: https://hubs.li/H0R6XzH0
Hamilton, 2021
In this Case Study, Genuity Science, a privately-funded genomics company, explains the benefits o... more In this Case Study, Genuity Science, a privately-funded genomics company, explains the benefits of automating NGS workflows for trio analysis in patients with rare diseases
Highlights of this case study:
- Strategies for the genetic analysis of rare diseases
- From manual to automated NGS workflows: motivations and benefits
- Automating NGS workflows for diagnosis on Hamilton instruments: from DNA quantification to library pooling
Link for download of free Case Study: https://hubs.ly/H0Dcf6j0
Hamilton, 2020
In this case study, Biobank Graz, one of the largest clinical biobanks in Europe, explains the be... more In this case study, Biobank Graz, one of the largest clinical biobanks in Europe, explains the benefits of automating the processing and storage of liquid samples from patients recovered from COVID-19. Highlights of this case study: - The role of biobanks in medical research - From manual to automated sample processing and storage: Needs and motivations - Automated processing and storage of liquid samples: Systems description and benefits Link to the (free) Case Study: https://hubs.ly/H0BHQzN0
Hamilton, 2020
In this case study, CeGaT, an innovative diagnostics company in Germany, explains the benefits of... more In this case study, CeGaT, an innovative diagnostics company in Germany, explains the benefits of automating the workflows for NGS library preparation for the analyses of Tumor Mutational Burden (TMB) and Microsatellite Instability (MSI).
Highlights of this case study:
-Medical relevance of TMB and MSI analysis
-From manual to automated NGS library preparation: needs and motivations
-Automated NGS library preparation: system description and benefits
Link to the (free) Case Study: https://hubs.ly/H0xYng-0
Hamilton, 2020
In this case study, Dr. Mosé Barbaro, a biologist at the Pharmacotoxicology Laboratory of the Civ... more In this case study, Dr. Mosé Barbaro, a biologist at the Pharmacotoxicology Laboratory of the Civil Hospital of Brescia, explains the benefits of an automated LC-MS workflow with the Hamilton MassSTAR in the analysis of immunosuppressants.
Highlights of this case study:
-Therapeutic Drug Monitoring analyses and their medical relevance
-From manual to automated testing for immunosuppressants: Needs and motivations
-Automated sample preparation for LC-MS analysis: system description and benefits
Link to the (free) Case Study: https://hubs.ly/H0vSkrj0
Scientific reports, 2019
Translation of pharmacological results from in vitro cell testing to clinical trials is challengi... more Translation of pharmacological results from in vitro cell testing to clinical trials is challenging. One of the causes that may underlie these discrepant results is the lack of the phenotypic or species-specific relevance of the tested cells; today, this lack of relevance may be reduced by relying on cells differentiated from human pluripotent stem cells. To analyse the benefits provided by this approach, we chose to focus on Friedreich ataxia, a neurodegenerative condition for which the recent clinical testing of two compounds was not successful. These compounds, namely, resveratrol and nicotinamide, were selected because they had been shown to stimulate the expression of frataxin in fibroblasts and lymphoblastoid cells. Our results indicated that these compounds failed to do so in iPSC-derived neurons generated from two patients with Friedreich ataxia. By comparing the effects of both molecules on different cell types that may be considered to be non-relevant for the disease, such as fibroblasts, or more relevant to the disease, such as neurons differentiated from iPSCs, a differential
response was observed; this response suggests the importance of developing more predictive in vitro systems for drug discovery. Our results demonstrate the value of utilizing human iPSCs early in drug discovery to improve translational predictability.
Scientific reports, 2015
Spinal muscular atrophy (SMA) is a neuromuscular disease caused by mutations in Survival Motor Ne... more Spinal muscular atrophy (SMA) is a neuromuscular disease caused by mutations in Survival Motor Neuron 1 (SMN1), leading to degeneration of alpha motor neurons (MNs) but also affecting other cell types. Induced pluripotent stem cell (iPSC)-derived human MN models from severe SMA patients have shown relevant phenotypes. We have produced and fully characterized iPSCs from members of a discordant consanguineous family with chronic SMA. We differentiated the iPSC clones into ISL-1+/ChAT+ MNs and performed a comparative study during the differentiation process, observing significant differences in neurite length and number between family members. Analyses of samples from wild-type, severe SMA type I and the type IIIa/IV family showed a progressive decay in SMN protein levels during iPSC-MN differentiation, recapitulating previous observations in developmental studies. PLS3 underwent parallel reductions at both the transcriptional and translational levels. The underlying, progressive developmental decay in SMN and PLS3 levels may lead to the increased vulnerability of MNs in SMA disease. Measurements of SMN and PLS3 transcript and protein levels in iPSC-derived MNs show limited value as SMA biomarkers.
J Control Release, 2018
Integration-deficient lentiviral vectors (IDLVs) are promising gene delivery tools that retain th... more Integration-deficient lentiviral vectors (IDLVs) are promising gene delivery tools that retain the high trans-duction efficiency of standard lentiviral vectors, yet fail to integrate as proviruses and are instead converted into episomal circles. These episomes are metabolically stable and support long-term expression of transgenes in non-dividing cells, exhibiting a decreased risk of insertional mutagenesis. We have embarked on an extensive study to compare the transduction efficiency of IDLVs pseudotyped with different envelopes (vesicular stomatitis, Rabies, Mokola and Ross River viral envelopes) and self-complementary adeno-associated viral vectors, serotype-9 (scAAV-9) in spinal cord tissues after intraspinal injection of mouse embryos (E16). Our results indicate that IDLVs can transduce motor neurons (MNs) at extremely high efficiency regardless of the envelope pseudotype while scAAV9 mediates gene delivery to ~40% of spinal cord motor neurons, with other non-neuronal cells also transduced. Long-term expression studies revealed stable gene expression at 7 months post-injection. Taken together, the results of this study indicate that IDLVs may be efficient tools for in utero cord transduction in therapeutic strategies such as for treatment of inherited early childhood neurodegenerative diseases.
Review, unpublished, 2012
The discovery of induced pluripotent stem cells (iPSCs) in 2006 has inspired almost all areas of ... more The discovery of induced pluripotent stem cells (iPSCs) in 2006 has inspired almost all areas of the life sciences. In the first report about iPSCs from Takahashi & Yamanaka, terminally differentiated cells (adult skin fibroblasts) were reprogrammed into pluripotent cells - very similar to embryonic stem cells (ESCs) - through the ectopic expression of four transcription factors: OCT3/4, SOX2, c-MYC and KFL4. Like ESCs, iPSCs have unlimited capacity to renew themselves; they also can differentiate into any other cell type (e.g. muscle cells, neurons). Since their discovery, an unprecedented high number of research groups have taken up this revolutionising technology, and generated iPSCs from patients with genetic diseases. Patient-specific iPSCs offer a new way to study the diseases on cell types which were not accessible for in vitro studies before: neurons from patients with neurological diseases such as Parkinson’s or Alzheimer’s disease, muscle cells from patients suffering from muscular dystrophy, diseases affecting retina cells, and many more examples. These cells provide an unlimited source of human material for modelling the disease and may facilitate screening for novel drugs. Moreover, using iPSCs for cellular replacement therapy or individualized cell therapy after genetic correction may become a viable option when general obstacles of cellular therapies can be overcome. This review summarizes and comments on approaches that have been taken in the last six years for the study and treatment of human diseases using iPSCs.
Business/ Strategy reports by Gabriela Boza-Moran
Report- Debiopharm International , 2016
Since the introduction of the Orphan Drug Designation (ODD) in 1983, the amount of investment and... more Since the introduction of the Orphan Drug Designation (ODD) in 1983, the amount of investment and the level of activity in orphan drug (OD) development plans have exponentially increased. While less than 10 ODs reached the market between 1973 and 1983, we now have more than 500 ODs (accounting for compounds that are approved for more than one orphan indication), some of which have reached blockbuster status (sales >$1 billion). The large media coverage dedicated to the successful stories have however failed to recognize the unique challenges that ODs encounter during the reimbursement process after marketing authorization (MA). While the main mandate of regulatory bodies such as the Food and Drug Administration (FDA) in the US and the European Medicines Agency (EMA) in the EU is to assess the drug’s safety and efficacy, the main mandate of Health Technology Assessment (HTA)/reimbursement bodies and payers is to assess the level of added benefit and its relationship to its costs (cost-effectiveness). Furthermore, although MA in the EU is granted by a centralized agency, the reimbursement decisions are made separately by each country, and sometimes by each region...
Business Plan. Executive MBA EPFL Switzerland , 2016
Authentique will deliver personalized nutrition plans and lunch meals to busy, health-conscious i... more Authentique will deliver personalized nutrition plans and lunch meals to busy, health-conscious individuals in order to help them fulfill their fitness needs and goals. Our target customers are 30-45 years old time-poor professionals with high disposable incomes, residing/working in the Geneva region. Initially, we envision a distribution strategy by forming partnerships with five specific gym sites to act as points of sales, thus simplifying logistics and building a brand consistent with health. This will be followed by an expansion to offices, sporting events and ultimately other cities. A combination of market intelligence research, focus groups, surveys and interviews with prospective customers and fitness industry professionals has reinforced the
assumption that there is an unmet need for an affordable, tasty and hassle free nutritional alternative. The cornerstone of our business is the development of the AUTHENTIQUE® brand. This will be achieved through partnerships and a distribution strategy with selected gyms. Furthermore, we will carry out creative marketing in social media, local news and print media to reach our customers. Our streamlined and state of the art operation’s planning has been optimized post discussion with experts in the field and high quality products will deliver our customer centric vision. Our vision is to enable our customers to achieve their goals, understand their needs and offer a convenient food ordering service. The business will be financed in two rounds with 200,000 CHF in equity in year one (100,000 CHF founder’s equity and 100,000 CHF
from a business angel) and a 150,000 CHF bank loan in the second year. Authentique expects to reach breakeven on year three, and to fully pay back the bank loan on year 6.
Thesis Chapters by Gabriela Boza-Moran
PhD Thesis, 2013
Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disease in which low levels... more Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disease in which low levels of survival of motor neuron (SMN) protein lead to the degeneration of alpha motor neurons (MNs) in the spinal cord. The pathological mechanism of SMA is highly controversial and until recently it was not possible to obtain human MNs to study the disease. The development of induced pluripotent stem cell (iPSC) technology has made it possible to bypass this obstacle and iPSC-based models of SMA type I have already been validated by two separate groups. Encouraged by these pioneering findings I have produced and characterized iPSCs from several members of a discordant consanguineous family in which four haploidentical siblings share the same homozygous SMN1 mutation, but nonetheless show different phenotypes of the disease. I have differentiated iPSC clones from three of the siblings and the carrier unaffected mother, as well as a control unaffected clone and a clone derived from a patient with SMA type I, into ISL1+/ChAT+ MNs. No obvious phenotypic difference was observed between the MN cultures of the siblings during the period of study, but cells from the SMA type I patient did show an impaired ability to form rosettes. The study of SMN and PLS3 levels during the differentiation from iPSCs to ChAT+ MNs showed a gradual decay of these proteins during MN development in all clones. Furthermore, SMN protein levels did not correlate with the pattern of mRNA expression, suggesting the existence of post-transcriptional and/or post-translational regulation of full-length SMN (FL-SMN) transcripts and protein. The FL/Δ7-SMN mRNA ratio and total SMN (tSMN) mRNA levels were found to be possible biomarkers to distinguish unaffected individuals from SMA patients and the severity of SMA pathology, respectively. PLS3 protein level was higher in the SMA type IV/asymptomatic sibling than in two of the type III SMA siblings, but it could not be confirmed as a modifier factor in the
family. These results suggest that SMN levels are regulated during MN development, and that low levels may impair the generation of rosettes but not necessarily of MNs. SMN levels in MNs only show minor differences between patients, suggesting that there may be a threshold after which reduced levels of SMN within a narrow range become suddenly and increasingly detrimental unless modifier factors can compensate for the cellular function(s) lost.
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Scientific Articles by Gabriela Boza-Moran
Highlights of this Case Study:
- Description of a highly integrated system consisting of thirteen instruments, including a Hamilton Verso® Compound Management System and two Hamilton Microlab® STAR(let) liquid handlers
- Detailed description of the automated methods for compound dissolution and aliquoting, compound (un)loading to/from -20 °C storage, and compound reformatting
- Data exchange among instruments and use of the Hamilton VENUS® software as the central hub
Link to free Case Study: https://hubs.li/Q02Z_DMg0
Highlights of this Case Study
- Overview of the analytical methods automated by AGC Biologics Milan on Hamilton liquid Handlers
- Detailed description of two ELISA-based automated workflows to assess the purity of the lentiviral vectors: residual benzonase and residual Host-Cell Proteins (HCP)
- Advantages of the Hamilton VENUS Scheduler to plan and execute parallel assays using the same sample aliquot
Link to Case Study: https://hubs.li/Q01CV6MV0
Highlights of this Case Study
- Detailed description of the automated system used for the cell-based workflow
- Advantages of Hamilton's CECULA Graphical User Interface (GUI) for the planning of long-term cell-based assays
- Transfer of the automated assay to Good Manufacturing Practice (GMP)
Link to free Case Study: https://hubs.li/Q01xk41G0
Highlights of this Case Study:
· NHLS COVID-19 Testing Strategy in South Africa
· Description of the automated SARS-COV-2 Testing and its benefits
· Beyond Testing: SARS-COV-2 surveillance via NGS sequencing
Link to free Case Study: https://hubs.li/Q011-ySB0
Highlights of this Case Study:
The role of BioBank Dresden in Cancer Research
From manual to automated sample processing: challenges and benefits
Detailed system and workflow description
Link to free Case Study: https://hubs.li/H0_5RPv0
Highlights of this case study:
• Benefits of using oral fluid samples as a matrix for LC-MS analysis
• Description of a new LC-MS sample prep method for DoA testing in oral fluid samples
• Benefits of developing this new testing method using automation
Link to free Case Study: https://hubs.li/H0R6XzH0
Highlights of this case study:
- Strategies for the genetic analysis of rare diseases
- From manual to automated NGS workflows: motivations and benefits
- Automating NGS workflows for diagnosis on Hamilton instruments: from DNA quantification to library pooling
Link for download of free Case Study: https://hubs.ly/H0Dcf6j0
Highlights of this case study:
-Medical relevance of TMB and MSI analysis
-From manual to automated NGS library preparation: needs and motivations
-Automated NGS library preparation: system description and benefits
Link to the (free) Case Study: https://hubs.ly/H0xYng-0
Highlights of this case study:
-Therapeutic Drug Monitoring analyses and their medical relevance
-From manual to automated testing for immunosuppressants: Needs and motivations
-Automated sample preparation for LC-MS analysis: system description and benefits
Link to the (free) Case Study: https://hubs.ly/H0vSkrj0
response was observed; this response suggests the importance of developing more predictive in vitro systems for drug discovery. Our results demonstrate the value of utilizing human iPSCs early in drug discovery to improve translational predictability.
Business/ Strategy reports by Gabriela Boza-Moran
assumption that there is an unmet need for an affordable, tasty and hassle free nutritional alternative. The cornerstone of our business is the development of the AUTHENTIQUE® brand. This will be achieved through partnerships and a distribution strategy with selected gyms. Furthermore, we will carry out creative marketing in social media, local news and print media to reach our customers. Our streamlined and state of the art operation’s planning has been optimized post discussion with experts in the field and high quality products will deliver our customer centric vision. Our vision is to enable our customers to achieve their goals, understand their needs and offer a convenient food ordering service. The business will be financed in two rounds with 200,000 CHF in equity in year one (100,000 CHF founder’s equity and 100,000 CHF
from a business angel) and a 150,000 CHF bank loan in the second year. Authentique expects to reach breakeven on year three, and to fully pay back the bank loan on year 6.
Thesis Chapters by Gabriela Boza-Moran
family. These results suggest that SMN levels are regulated during MN development, and that low levels may impair the generation of rosettes but not necessarily of MNs. SMN levels in MNs only show minor differences between patients, suggesting that there may be a threshold after which reduced levels of SMN within a narrow range become suddenly and increasingly detrimental unless modifier factors can compensate for the cellular function(s) lost.
Highlights of this Case Study:
- Description of a highly integrated system consisting of thirteen instruments, including a Hamilton Verso® Compound Management System and two Hamilton Microlab® STAR(let) liquid handlers
- Detailed description of the automated methods for compound dissolution and aliquoting, compound (un)loading to/from -20 °C storage, and compound reformatting
- Data exchange among instruments and use of the Hamilton VENUS® software as the central hub
Link to free Case Study: https://hubs.li/Q02Z_DMg0
Highlights of this Case Study
- Overview of the analytical methods automated by AGC Biologics Milan on Hamilton liquid Handlers
- Detailed description of two ELISA-based automated workflows to assess the purity of the lentiviral vectors: residual benzonase and residual Host-Cell Proteins (HCP)
- Advantages of the Hamilton VENUS Scheduler to plan and execute parallel assays using the same sample aliquot
Link to Case Study: https://hubs.li/Q01CV6MV0
Highlights of this Case Study
- Detailed description of the automated system used for the cell-based workflow
- Advantages of Hamilton's CECULA Graphical User Interface (GUI) for the planning of long-term cell-based assays
- Transfer of the automated assay to Good Manufacturing Practice (GMP)
Link to free Case Study: https://hubs.li/Q01xk41G0
Highlights of this Case Study:
· NHLS COVID-19 Testing Strategy in South Africa
· Description of the automated SARS-COV-2 Testing and its benefits
· Beyond Testing: SARS-COV-2 surveillance via NGS sequencing
Link to free Case Study: https://hubs.li/Q011-ySB0
Highlights of this Case Study:
The role of BioBank Dresden in Cancer Research
From manual to automated sample processing: challenges and benefits
Detailed system and workflow description
Link to free Case Study: https://hubs.li/H0_5RPv0
Highlights of this case study:
• Benefits of using oral fluid samples as a matrix for LC-MS analysis
• Description of a new LC-MS sample prep method for DoA testing in oral fluid samples
• Benefits of developing this new testing method using automation
Link to free Case Study: https://hubs.li/H0R6XzH0
Highlights of this case study:
- Strategies for the genetic analysis of rare diseases
- From manual to automated NGS workflows: motivations and benefits
- Automating NGS workflows for diagnosis on Hamilton instruments: from DNA quantification to library pooling
Link for download of free Case Study: https://hubs.ly/H0Dcf6j0
Highlights of this case study:
-Medical relevance of TMB and MSI analysis
-From manual to automated NGS library preparation: needs and motivations
-Automated NGS library preparation: system description and benefits
Link to the (free) Case Study: https://hubs.ly/H0xYng-0
Highlights of this case study:
-Therapeutic Drug Monitoring analyses and their medical relevance
-From manual to automated testing for immunosuppressants: Needs and motivations
-Automated sample preparation for LC-MS analysis: system description and benefits
Link to the (free) Case Study: https://hubs.ly/H0vSkrj0
response was observed; this response suggests the importance of developing more predictive in vitro systems for drug discovery. Our results demonstrate the value of utilizing human iPSCs early in drug discovery to improve translational predictability.
assumption that there is an unmet need for an affordable, tasty and hassle free nutritional alternative. The cornerstone of our business is the development of the AUTHENTIQUE® brand. This will be achieved through partnerships and a distribution strategy with selected gyms. Furthermore, we will carry out creative marketing in social media, local news and print media to reach our customers. Our streamlined and state of the art operation’s planning has been optimized post discussion with experts in the field and high quality products will deliver our customer centric vision. Our vision is to enable our customers to achieve their goals, understand their needs and offer a convenient food ordering service. The business will be financed in two rounds with 200,000 CHF in equity in year one (100,000 CHF founder’s equity and 100,000 CHF
from a business angel) and a 150,000 CHF bank loan in the second year. Authentique expects to reach breakeven on year three, and to fully pay back the bank loan on year 6.
family. These results suggest that SMN levels are regulated during MN development, and that low levels may impair the generation of rosettes but not necessarily of MNs. SMN levels in MNs only show minor differences between patients, suggesting that there may be a threshold after which reduced levels of SMN within a narrow range become suddenly and increasingly detrimental unless modifier factors can compensate for the cellular function(s) lost.
particular interés, es el polimorfismo Val34Leu (FXIIIval34leu), en el cual ocurre un transversión (G/T), que se traduce en el cambio de una valina por una leucina en la posición 34 de la proteína, y cuya prevalencia tiene una alta heterogeneidad étnica, alcanzado porcentajes de hasta el 52% en los países occidentales y 1% en poblaciones asiáticas. El papel de este polimorfismo ha sido controversial. Algunos estudios de asociación genética han encontrado que la presencia del alelo mutado del polimorfismo (FXIII34leu) está asociada con un efecto protector para el desarrollo de
IAM, mientras que otros estudios señalan que tiene un papel neutro o inclusive protrombótico.
Recientemente se ha sugerido sin embargo, que su efecto está modulado por otros factores como el fibrinógeno y la aspirina. En este sentido, el objetivo de este trabajo de grado fue determinar la presencia del FXIIIVal34Leu en pacientes que ha sufrido IAM y en pacientes controles y establecer así su relación independiente y/o modulada con la patología. Para ello se tomaron muestras de sangre de cada individuo mediante la técnica de vasopunción, se aisló el ADN genómico y se amplificó la región polimórfica de interés, para posteriormente detectar la presencia del polimorfismo mediante el análisis por RFLP con la enzima HhaI. Los resultados obtenidos indican que no hay una asociación estadísticamente significativa entre el FXIIIval34leu
y el IAM, siendo la edad, el sexo y el hábito tabáquico los principales factores de riesgo. Para el genotipo heterócigo se observa sin embargo, una importante tendencia de protección contra el desarrollo de la enfermedad. En cuanto a las variables moduladoras, el efecto de la concentración de fibrinógeno no pudo establecerse claramente en función a los datos disponibles, al igual que el efecto de la toma de aspirina. Sin embargo, se observó una tendencia de protección para el IAM
en los individuos portadores del alelo mutado con un aumento relativo en las concentraciones de fibrinógeno plasmático, estimado de manera indirecta a través de las variables predictivas de edad y hábito tabáquico. Una tendencia similar se observó en los individuos que toman aspirina.
A pesar de las limitantes en cuanto a la disponibilidad de datos, de este estudio se deriva la proposición un modelo trombina-fibrinógeno-FXIII que explica tentativamente el efecto de la variante polimórfica dentro de un contexto dinámico, modelo que puede ser corroborado bajo un esquema metodológico simple propuesto también en este trabajo.
Todas estas situaciones conllevan a una disminución del autoestima, a un decaimiento afectivo que presenta gran número de síntomas depresivos que se manifiestan con mucha frecuencia producto de las misma etapa de maduración que se esta viviendo, y estos síntomas pueden afectar de manera contundente la vida del individuo a nivel familiar, a nivel social y a nivel académico. A fin de poder determinar esa situación en una muestra determinada, se aplicó un test y una encuesta. El primero es una escala de autoevaluación de la depresión SDS de William Zung, que mide la sintomatología depresiva sin o con patología indicando cuales son los síntomas que pueden ser significativos. La segunda es una encuesta de preguntas abiertas y cerradas que indaga un poco acerca de la posible existencia de estados depresivos, de sus influencias en el ámbito familiar, social y académico y de su conocimiento en la muestra.
Para la realización de este proyecto se trabajó con 112 alumnas de sexo femenino, cursantes de 1er año de Ciencias, 1er año de Humanidades, 2do año de Ciencias y 2do Año de Humanidades de la Unidad Educativa Colegio Concepción Terrazas del Club Hípico, cuyas edades oscilan entre los 15 y 18 años. Luego de pasar el test y la encuesta, los datos de las mismas fueron analizados estadísticamente, tabulados y graficados a fin de establecer una conclusión y dar respuesta a la hipótesis inicial. Los resultados demostraron la presencia de síntomas depresivos en la muestra, identificándose como predominantes a los síntomas depresivos referidos a alteraciones psicológicas y alteraciones psicomotoras. Así mismo la muestra reportó que los síntomas depresivos reconocidos tienen una influencia importante en el campo familiar y social, perdiendo relevancia en el ámbito escolar.
neuromuscular disease in which the genetic defect is
known, but the pathological mechanism is still both unclear
and controversial. Primary motor neurons (MNs) cannot be
obtained from living individuals, but induced pluripotent
stem cell (iPSC) technology has recently allowed the
production of human MNs in culture1. We have generated
iPSCs from several members of a discordant SMA type III
family who carry an SMN1 mutation (Figure 1). All iPSC
clones tested are able to differentiate similarly into HB9+/
ChAT+ MNs. We have not detected significant differences
in protein levels of SMN and plastin3 between iPSC-derived
MNs of the family members. Further imaging, transcriptomic
and proteomic analyses are ongoing.
Authentique will deliver personalized nutrition plans and lunch meals to busy, health-conscious individuals in order to help them fulfill their fitness needs and goals. Our target customers are 30-45 years old time-poor professionals with high disposable incomes, residing/working in the Geneva region. Initially, we envision a distribution strategy by forming partnerships with five specific gym sites to act as points of sales, thus simplifying logistics and building a brand consistent with health. This will be followed by an expansion to offices, sporting events and ultimately other cities. A combination of market intelligence research, focus groups, surveys and interviews with prospective customers and fitness industry professionals has reinforced the
assumption that there is an unmet need for an affordable, tasty and hassle free nutritional alternative. The cornerstone of our business is the development of the AUTHENTIQUE® brand. This will be achieved through partnerships and a distribution strategy with selected gyms. Furthermore, we will carry out creative marketing in social media, local news and print media to reach our customers. Our streamlined and state of the art operation’s planning has been optimized post discussion with experts in the field and high quality products will deliver our customer centric vision. Our vision is to enable our customers to achieve their goals, understand their needs and offer a convenient food ordering service. The business will be financed in two rounds with 200,000 CHF in equity in year one (100,000 CHF founder’s equity and 100,000 CHF
from a business angel) and a 150,000 CHF bank loan in the second year. Authentique expects to reach breakeven on year three, and to fully pay back the bank loan on year 6.
As with any other therapeutic product, the development and manufacturing of viral vectors for gene therapy must follow Good Practice (GxP) regulations. A Good Manufacturing Practice (GMP)-compliant process for viral vectors must ensure consistent quality in the vector’s potency (biological activity), purity (absence of contaminants or impurities), and safety (absence of potentially harmful substances). To achieve this, various “Critical Quality Attributes (CQAs)” are monitored during the manufacturing process by measuring key properties and ensuring that they are within the appropriate levels. Quick, reliable, and high-throughput analytical methods to assess these CQAs are essential in process development and later GMP-compliant testing for batch release during Quality Control. Given that automated liquid handlers dramatically improve results’ reproducibility and samples’ traceability, they are inherently suitable for high-level regulatory compliance.
This eBook is part of a dedicated campaign on Analytical Methods for Viral Vector Development and Manufacturing in Gene Therapy. Our scientific campaigns aim to provide our readers with interesting educational resources and a close-up view of how customers use our solutions to accomplish their tasks.
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Several cell culture types and cell-based systems are currently used for cell-based assays; from traditional 2-dimensional (2D) monolayers to scaffold-based 3D culture (e.g. organoids) and, more recently, Organs-On-A-Chip (OOAC). The maintenance and handling of these cell cultures require significant expertise and time. Automated liquid handling systems can ensure the standardization of the entire process by controlling critical aspects such as the speed of aspiration and dispensing, the position of the tip within the well, the tilting of the plates during pipetting steps, the temperature of reagents on-deck, and the sterility of the working area. Furthermore, automated liquid handling systems can significantly increase throughput via 96 and 384 multi-probe heads, and integrate third-party devices to perform cell imaging.
In this eBook, we describe the various cell-based models currently used in research and discuss how cell-based high-throughput screening (HTS) requirements can be fulfilled using Hamilton automated liquid handlers.
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Overall, the selection of the most suitable liquid handling technology for a laboratory is dependent upon; (i) throughput, (ii) complexity of the workflow, (iii) available budget, (iv) laboratory space and requirements for (v) sterility and measures to control cross-contamination, (vi) traceability, and (vii) precision. Hamilton Robotics offers three entry-level automated systems: Microlab® Prep, NIMBUS Line and Replicator Line.
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vaccines. Whereas in the past, most vaccines were based on inactivated or weakened
(i.e. live-attenuated) forms of the pathogen, today, vaccines can be manufactured using
protein sub-units, toxins, viral vectors, monoclonal antibodies, recombinant DNA, and
recombinant RNA.
Regardless of the type of vaccine, the endpoints used to measure their efficacies are the
same: prevention from infection, transmission, disease, and death. Most of the laboratory
tests used by clinicians to measure the occurrence of current and past infection and the
levels and types of immunogenicity can be automated. Automation-friendly assays include
Enzyme-Linked Immunosorbent Assays (ELISA), flow cytometry, neutralization assays,
and quantitative PCR (qPCR).
In this eBook, we discuss the key milestones achieved throughout the history of vaccine
development, the laboratory tests commonly used to assess vaccines’ efficacy, and we
describe how the latter can be automated using Hamilton solutions.
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The type of LC-MS analysis performed depends on the type of molecule and the questions being investigated. Moreover, each type of molecule and matrix requires its own protocol for sample preparation. Automation allows for the standardization of all the steps of the workflow, while also significantly increasing the capacity for sample processing.
In this eBook, we discuss the use of LC-MS in (bio)pharmaceutical research, the drivers behind the need for automation during sample preparation for LC-MS and the solutions that Hamilton offers to customers working in this field.
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The remarkable developments of molecular technologies have allowed for faster, more accurate and more efficient diagnostic methods. Whereas traditional methods are based on the culture of pathogens, molecular methods are based on the identification of the genomic material unique to each pathogen. Current molecular diagnostic methods are based on various types of techniques including qPCR, Next-Generation Sequencing (NGS) and microarrays. The majority of the workflows developed for these techniques can be automated to improve variables such as standardization, sample traceability and
throughput.
In this eBook, we discuss the impact of modern molecular diagnostic technologies on patient treatment and disease management, the drivers behind the need for automating the sample preparation workflows of molecular diagnostics methods and the solutions that Hamilton offers to customers working in this field.
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Many hospitals do not (yet) perform a large number of NGS tests to diagnose rare diseases. The need for automation, nevertheless, has been driven by two main factors: the high sample throughput in several national large-scale sequencing projects and the need for high accuracy and reproducibility. In this eBook, we discuss the impact NGS is having on the diagnosis of rare diseases, the drivers behind the need for automation during sample preparation for NGS and the solutions that Hamilton offers to customers working in this field.
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analyze large sets of data.
The explosive growth in the biobanking sector changed its organization and dynamics. Biobanking has evolved into decentralized complex infrastructures with large repositories of heterogeneous samples. Biobanking workflows need to be standardized and scaled up in order to harmonize biobanking activities among laboratories and ensure the quality of the samples. Automation allows for both, while also catering for various levels of regulatory compliance.
In this eBook, we discuss the evolution of biobanking activities, the drivers behind the need for automation during sample processing and storage, and the solutions that Hamilton Robotics and Hamilton Storage offer to customers working in this field.
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In this eBook, we discuss the impact of LC-MS in TDM, the drivers behind the need for automation during sample preparation for LC-MS and the solutions that Hamilton offers to customers working in this field.
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At Hamilton, we have closely followed the trends and impact of NGS in the oncology field and we have been quick to recognize our customer's automation needs in order to adopt this technology. In this eBook, we discuss the impact of NGS in precision medicine (PM)-oncology, the drivers behind the need for automation during the sample preparation for NGS and the solutions that Hamilton offers to customers working in this field.
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