Introduction to Fermentation
Technology
• Fermentation in Traditional and Modern
Practices
• Key Differences Between Fermentation and
Other Bioprocesses
Fermentation in Traditional and
Modern Practices
• • Used in food preservation (e.g., pickling,
brewing, dairy)
• • Modern fermentation applies bioreactors,
genetic engineering
• • Used in pharmaceuticals, biofuels, and
industrial chemicals
Key Differences Between
Fermentation and Other
Bioprocesses
• • Fermentation occurs under controlled
anaerobic/aerobic conditions
• • Other bioprocesses involve enzymatic
reactions, cell cultures
• • Fermentation relies on microbial metabolism
Types of Fermentation Processes
• Mixed Fermentation (Combination of Aerobic
& Anaerobic)
• Factors Affecting Fermentation Efficiency
Mixed Fermentation
• • Uses both aerobic and anaerobic conditions
• • Example: Vinegar production – yeast
(anaerobic) produces ethanol, Acetobacter
(aerobic) converts ethanol to acetic acid
Factors Affecting Fermentation
Efficiency
• • Substrate Composition
• • Temperature & pH
• • Oxygen Levels
• • Sterility & Contamination Prevention
Industrial Applications of
Fermentation
• Fermentation in Enzyme Production
• Fermentation in Agricultural Biotechnology
Fermentation in Enzyme
Production
• • Produces industrial enzymes (amylase,
protease, lipase)
• • Used in food processing, detergents,
biofuels
Fermentation in Agricultural
Biotechnology
• • Produces biofertilizers, biopesticides (e.g.,
Bacillus thuringiensis)
• • Enhances soil health, reduces chemical use
Examples of Fermentation
Products
• Citric Acid Fermentation
• Probiotic Fermentation
Citric Acid Fermentation
• • Produced by Aspergillus niger in submerged
fermentation
• • Used in food, pharmaceuticals, cosmetics
Probiotic Fermentation
• • Lactobacillus, Bifidobacterium fermentation
• • Improves gut health, boosts immunity, used
in functional foods
Challenges in Fermentation
Technology
• Contamination and Sterility Issues
• Cost and Scalability Concerns
Contamination and Sterility Issues
• • Reduces yield and product purity
• • Requires stringent sterilization techniques
Cost and Scalability Concerns
• • High costs for controlled environments
• • Scaling from lab to industry presents
efficiency challenges
Future Trends in Fermentation
Technology
• AI and Automation in Fermentation
• Synthetic Biology’s Role in Future
Fermentation
AI and Automation in Fermentation
• • AI-driven monitoring improves process
control
• • Automation reduces errors, increases
industrial productivity
Synthetic Biology’s Role in Future
Fermentation
• • Engineered microbes for custom
fermentation products
• • Synthetic biology enables precise metabolic
modifications