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Cell Disruption Methods Table

The document outlines various cell and tissue disruption methods, detailing their advantages and disadvantages. Techniques such as Mortar and Pestle, Glass Homogenizers, Bead and Vortexing, Dounce Homogenizer, Potter-Elvehjem, French Press, Ultrasonic Homogenization, and Freeze/Thawing are discussed. Each method has specific applications, benefits, and limitations, particularly regarding sample size, tissue type, and reproducibility.

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selma bouteldja
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53 views1 page

Cell Disruption Methods Table

The document outlines various cell and tissue disruption methods, detailing their advantages and disadvantages. Techniques such as Mortar and Pestle, Glass Homogenizers, Bead and Vortexing, Dounce Homogenizer, Potter-Elvehjem, French Press, Ultrasonic Homogenization, and Freeze/Thawing are discussed. Each method has specific applications, benefits, and limitations, particularly regarding sample size, tissue type, and reproducibility.

Uploaded by

selma bouteldja
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

CELL DISRUPTION METHODS

Table 2. Advantages and disadvantages of different cell and tissue disruption methods.
Method Advantages Disadvantages
 Inexpensive  Small sample only
 Easy to use and clean  Only one sample at a time
 Efficient homogenization of most tissues  Requires multiple sets
 Only frozen tissues/cells
Mortar and Pestle  Tedious
 Homogenization may not be reproducible
 Cross-contamination is possible

 Inexpensive  Not recommended for fibrous tissue


 Easy to keep chilled (muscle)
Glass Homogenizers  Effective with most tissues  Only one sample at a time
 May break easily

 Recommended for microorganisms and  Limited homogenization of solid samples


soft tissues  Expensive
 Homogenization of multiple samples
(high throughput)
Bead and Vortexing  No cross-contamination
 No sample loss
 Minimal sample heating
 Highly reproducible

 Inexpensive  May break easily


 Easy to use and clean  Not effective for intact pieces of
tissue
Dounce  Tedious
Homogenizer  May be irreproducible due to human
error

 Inexpensive  Not recommended for intact tissue


 Easy to use and clean samples
Potter-Elvehjem  Recommended for animal cells
with PTFE  One sample at a time
 Uniform homogenization

 Ideal for microorganisms like bacteria or  Expensive and difficult to clean


tissue that has been previously  Only small sample volumes
homogenized  Not for intact tissue
French Press  Uniform homogenization  Cross-contamination is possible
 Generates heat that might affect protein

 Recommended for cell suspensions,  Not recommended for tougher tissues


microorganisms and some tissues (muscle)
Ultrasonic  Ideal to tear subcellular organelles  Generates heat leading to protein
Homogenization  Quick and efficient process denaturation
 Expensive and requires
maintenance

 Ideal for bacteria, mammalian cells and  Time consuming


Freeze/Thawing yeast  Potential protein denaturation
 Inexpensive and easy to perform

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