Scribd
Upload
93 views13 pages

Ti PLASMID

The document discusses the Ti plasmid, a DNA molecule found in the plant pathogen Agrobacterium tumefaciens, which induces crown gall tumors in plants. It outlines the structure and organization of the Ti plasmid, the process of T-DNA transformation and integration, and the applications of Agrobacterium-mediated plant transformation for genetic modification. Additionally, it highlights limitations such as size, lack of unique restriction sites, and dependency on specific plant species for infection.

Uploaded by

rajalaxmiswain2005
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
93 views13 pages

Ti PLASMID

The document discusses the Ti plasmid, a DNA molecule found in the plant pathogen Agrobacterium tumefaciens, which induces crown gall tumors in plants. It outlines the structure and organization of the Ti plasmid, the process of T-DNA transformation and integration, and the applications of Agrobacterium-mediated plant transformation for genetic modification. Additionally, it highlights limitations such as size, lack of unique restriction sites, and dependency on specific plant species for infection.

Uploaded by

rajalaxmiswain2005
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

Ti

PLASMID
PRESENTED BY:
RAJALAXMI
SWAIN
ROLL NO:
BW2322677
CONTENTS:
INTRODUCTION

ORGANIZATION AND STRUCTURE


T-DNA TRANSFORMATION AND INTEGRATION
AGROBACTERIUM-MEDIATED PLANT
TRANSFORMATION
APPLICATION

LIMITATION
DEFINATION :
Ti means tumour inducing plasmid is an
extra chromosomal molecule of DNA found commonly in plant
pathogen –Agrobacterium tumefaciens.

INTRODUCTION:
-In1970, Smith & Townsend postulated that a bacterium was the
causative agent of crown gall tumour.
-A. tumefaciens infects wounded tissues(dicot plant).
-It is the consequence of Transfer integration & expression of
genes present in Ti plasmid.
ORGANISATION &
STRUCTURE
 :
Approx. 100kbp-2Mbp size
 Regions of Ti plasmid
1.T-DNA REGION –biosynthesis of auxin, cytokinin and opine
Right & left border
[Link] REGION –for transfer of T-DNA into host
[Link] CATABOLISM REGION –Codes for proteins involve in uptake &
metabolism of opine
 Other regions are :
ORIGIN OF REPLICATION
CONJUGATION REGION
HORMONE SYNTHESIS REGION
T-DNA TRANSFORMATION &
INTEGRATION
Signal induction to agrobacterium

Attachment of agrobacterium to plant cell

Production of virulence proteins

Production of T-DNA strand

Transfer of T-DNA out of agrobacterium

Transfer of T-DNA into plant cell & integration


AGROBACTERIUM-MEDIATED
PLANT TRANSFORMATION
Development of Agrobacterium carrying the vector with the
desired gene

Identification of suitable ex plant

Co-culture of explants with agrobacterium

Killing of agrobacterium with a suitable antibiotic

Selection of transformed plant cells

Regeneration of whole plant


Application
 Used to introduce new genes into plant for genetic
modification.
 Helps in developing genetically modified crops with
improved traits.
 Facilities the creation of transgenic plants with enhanced
resistance to pests and diseases.
 Allow the production of plants with improved nutritional
profiles.
 Assist in studying gene function and plant biology through
gene expression analysis.
LIMITATION
 Large in size
 Absence of unique restriction enzyme sites.
 Phytohormones must be removed.
 Opine production lowers the plant yield so it should be
removed.
 Can’t replicate in [Link].
 Usually works only in plants that can be infected by
Agrobacterium tumefaciens.

You might also like