SIWES DEFENSE
PRESENTATION
BY: Ossai Ken Onyemaechi – UAT20/03/01/1322
DEPARTMENT: Chemical Sciences
PROGRAMME: BSc. Biochemistry
INSTITUTION: University Of Africa, Toru-orua
INDUSTRIAL PLACEMENT BASE: Pharez Hospital And
Diagnostics, Effurun, Delta state.
DEFENSE TOPIC: INSTITUTION SUPERVISOR:
ALT AND AST LABORATORY ANALYSIS DR. BENNETT VICTORIA
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TOPIC:
ALANINE
TRANSAMINASE(ALT) AND
ASPARTATE
TRANSAMINASE(AST)
P R E S E N TAT I O N F O C U S :
P R I N C I P L E S , M AT E R I A L S , P R O C E D U R E S , R E S U LT S ,
C O N C LU S I O N / I N F E R E N C E , A N D R E F E R E N C E .
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INDUSTRY BASE ORGANOGRAM
MEDICAL DIRECTOR
SURGEON MEDICAL RECEPTION
PHARMACY MATRON
LABOLATORY
HEAD OF CERTIFIED
CONSULTANT GOP REGISTARY TRAINED NURSE
DEPARTMENT
DRUG
NEUROSURGEON INFORMATION SCIENTIST MAINTENANCE JANITOR
CENTRE
COMPOUNDING PHLEBOTOMIST TRAINEES
UROLOGY UNIT
DISPENSING UNIT STAFFS AND CLEANERS
TRAINEES
RECORDS
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ACTIVITIES DONE
DURING SIWES
Malaria Parasite Test Full Blood Count (FBC)
Blood Glucose Test Blood Grouping Test
Packed Cell Volume (PCV) Genotyping Test
Liver Function Test (LFT) Pregnancy Test
Lipid Profile Test Hormonal Profile Test
Urinalysis E/U/CR
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PRINCIPLE OF ALT
AND AST ANALYSIS
ALT PRINCIPLE
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PRINCIPLE OF ALT
AND AST ANALYSIS
AST PRINCIPLE
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MATERIALS
NEEDED
ALT MATERIALS AST MATERIALS
TEST TUBES TEST TUBES
SAMPLE SERUM SAMPLE SERUM
ALT TEST KIT AST TEST KIT
DISTILLED WATER DISTILLED WATER
MICROPIPETTE AND TIPS MICROPIPETTE AND TIPS
BIOCHEMISTRY ANALYSER BIOCHEMISTRY ANALYSER
HANDGLOVE AND LAB COAT LAB HANDGLOVES AND LAB
INCUBATOR COAT
SODIUM HYDROXIDE INCUBATOR
SODIUM HYDROXIDE
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TEST PROCEDURE:
RANDOX KINETIC METHOD
Set up two test tubes(sample blank and
sample/test)
Aspirate 500 microliter of R1 into the two tubes
Aspirate 100 microliter of distilled water to
sample blank and 100 microliter of sample
serum/plasma to sample test.
Incubate for 30minutes
After 30 minutes, aspirate 500 microliter of R2 to
both testtubes
Stand at room temperature for 20 minutes,
Add 5mL of NaOH to all tubes and read
immediately with biochemistry analyser.
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TEST RESULT:
Interpreting results of liver enzyme tests involves
understanding normal and abnormal ranges,
providing valuable insights into liver health. Normal
ranges can vary between laboratories, and
healthcare providers consider these variations when
assessing results. Himel, N. H. (2022).
ALT REFERENCE RANGE: 0 - 12 U/L
AST REFERENCE RANGE: 0 – 12 U/L
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CONCLUSION
OR INFERENCE:
The AST:ALT ratio aids in pinpointing the origin of liver
inflammation. Elevated AST levels suggest alcohol-related
disease, cirrhosis, or non-hepatic causes like myopathy,
thyroid disease, or excessive exercise. On the other hand,
ALT-predominant elevations are typically associated with viral
hepatitis, fatty liver, autoimmune hepatitis, or liver toxicity from
medications or toxins. This ratio is valuable for a more focused
differential diagnosis in assessing liver health (Senior, J. R.
2012).
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REFERENCE
• Himel, N. H. (2022). Liver function tests: Significance of ALT
(Alanine aminotransferase) and AST (Aspartate
aminotransferase) as markers of liver injury, 2020.
• Senior, J. R. (2012). Alanine aminotransferase: a clinical and
regulatory tool for detecting liver injury–past, present, and
future. Clinical Pharmacology & Therapeutics, 92(3), 332-339.
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THANK
YOU FOR
LISTENING
THE END
