Nutritive Values and Amino Acids in Foods
Nutritive Values and Amino Acids in Foods
Community Medicine
Write the Nutritive value of given food.
Name the limiting amino acid.
Wheat(RDA=460/410 mgs per day)
• Nutritive value
Protein-11.81g, carbohydrate-71.2g, Energy-
346Kcal.
• Limiting amino acid-lysine & threonine
• Rich in vitaminD,Iron, Calcium, Phosphorus & other
minerals.
• Refined wheat flour(Maida) is flour minus husk,
poor in nutrients & fiber.(Vitamins & minerals)
Write the Nutritive value of given food.
Name the limiting amino acid.
Write the nutrients lost during washing & cooking.
What is parboiling
Rice:
• Nutritive value-
Protein 6.8g, Carbohydrate78.2g, Energy-345Kcal
• Limiting amino acid-Theronine
• Devoid of vitamins-A,D, C , calcium & iron & rich in vitamin-B group of
vitamins especially thiamine & amino acid lysine.
• Polishing, washing, cooking with excess of water & draining deprives
nutrients like Thiamine, riboflavin & niacin
• Using under milled rice than highly polished rice is advised.
• Parboiling is partial cooking in steam to preserve the nutritive value of
rice, by soaking the paddy in hot water at 65-70 deg C for 3 to 4 hours,
followed by draining the water ^ steaming the soaked paddy for 5-10
minutes, than dried & milled
Write the Nutritive value of given food.
Name the limiting amino acid.
Maize:
• Nutritive Value-
Protein 11.1g,Carbohydrate 66.2g,Energy-342Kcal.
Limiting Amino acid- lysine & tryptophan.
Rich in leucine which prevents conversion of
tryptophan in to niacin(causing pellagra)
Write the Nutritive value of given food.
Name the limiting amino acid.
Jowar(Sorghum):
• Nutritive Value: Protein-10.4g, Carbohydrate-72.6g,
Energy-349Kcal.
• Limiting Amino acid-Lysine & threonine.
Rich in leucine which prevents the conversion of
tryptophan in to niacin(causing pellagra)
Rich in Iron(4mg/100g).phosphorus.
Fungi (Aspergillus flavus) will infest during improper
storage & produce aflatoxins, which is a potent
hepatotoxins & carcinogenic)
Write the Nutritive value of given food.
Name the limiting amino acid.
Bajra(Pearl millet):
• Nutritive Value-Protein-11.6g, Carbohydrate-
67.5g, Energy-361Kcal.
• Limiting Amino acid-Lysine & threonine.
• Rich in B group of vitamins & minerals such as
calcium & iron.
Write the Nutritive value of given food.
Name the limiting amino acid.
Ragi:(cheapest Millet)
• Nutritive value-Protein 7.3g, Carbohydrate-72.0g,
Energy-328Kcal.
• Limiting amino acid-Lysine
• Rich in calcium(344mg),iron4mg/100gm)
• Advised for diabetics & obese.
• Used in multipurpose food.
Write the Nutritive value of given food or identify
this pulse known as poor’s man meat
Name the limiting amino acid.
Pulses:(Nutritive value)also called as poor man’s meat(RDA=40 gm)
Bengal gram- P-17.1g,ENERGY-360Kcal.
Red gram-P-22.3g, Energy-335Kcal.
Green gram-P-24.5, Energy-348Kcal.
Black gram-P-24g, Energy 347.
Peasdry-P19.7g, Energy-315Kcal.
Soyabin-P-43.2g,Energy-432Kcal.
Limiting amino acid-Methionine & cysteine, but rich in lysine, in minerals
iron & calcium& B group vitamins(riboflavin & thiamine).
Anti nutritional factors are phytates& tannins, are destroyed by heat.
Presence of high amount of certain sugars, like oligosacchridociated is
known to be associated with flatulence.
Germination increase the concentration of vitamin C & B complex.
Name the adulterant/toxicant & disease commonly
associated with given food item.
What preventive measure can be taken to prevent
the same.
Khesari dal-Lathyrus sativus.
Toxin- Beta oxalyl amino alanine(BOAA)-water soluble.
vitamin C prophylaxis
Banning of crop.
Removal of toxin by steeping method,(soaking in hot
water for 2 hours, than soaked water is drained off.
Paraboiling(for large scale, simple soaking in lime water
overnight followed by boiling, destroys the toxin.
Write the nutritive value .
Mention amino acid which is richly present
Mention the fungi which infest the given spot under improper storage
conditions and the toxins produced by the same.
• Fruits:(RDA=85 gms.)
• Protective, rich in cellulose, fiber & water, vitamins, minerals,
• Papaya & mango are excellent source of carotene.
• Orange & Guava & Amla are rich in Vitamin C.
• Watermelon, apple, apricots are rich in iron.
• Musambi,musk melon, peaches are rich in potassium.
• Calories- 74 in mango & 104 in banana.
• Seasonal & locally available fruits are advised for daily
consumption.
• Fibre in fruits help in preventing hyperglycaemia &
hyperlipidemia.
Comment on bio-availability of iron from given spot
and the reason of the same.
Write recommended quantity of given spot for an
adult and for a pregnant female
Green leafy vegetables(protective)
RDA= 50-100gms.
• Fibers helps in reduction of hyperglycemia &
hyperlipidemia.
• Rich source of carotene, calcium, iron & vitamin C.
• Good source of riboflavin & folic acid.
• Poor bioavailability of iron due to presence of oxalates &
phytates.
• 40 gram daily & 150 gram daily for pregnant woman.
What is the recommended requirement?
It is a rich source of?
By what process it is prepared from oil?
Cooking oil: (Fat=900 cals)
RDA=40 mgs for male & 20 gms for female.
Rich in fat soluble vitamins(A,E ,D & K).
Total fat intake should not exceed 20% of total energy.
Hydrogenated oil is vansapati.
Fat can be fortified with vitamin A & D.
Consumption of excess oil & saturated fatty acids leads
to risk of developing IHD & dyslipidemia.
Repeated heating the oil(trans fatty acids) liberates free
Write calories and iron content of the given spot.
Where does the iron content of given spot come from?
Vanaspati ghee
– It is produced by “hydrogenation” of vegetable oils. This
process increases levels of saturated fatty acids and trans
fatty acids.
– Linoleic acid, Linolenic acid, Arachidonic acid,
Eicosapentanoic acid
What is the basic function of given spot?
Excess consumption of the spot may lead to
what?
Coffee & Tea:
• Are stimulant & relieves fatigue.
• Contains caffeine, tannic acid & volatile oil.
• Energy per 100 ml is 100Kcal for coffee & 80 Kcal for
tea.(comes from milk & sugar).
• Excess consumption increase blood pressure, cause
insomania,tachycardia,gastritis.
• Anti-oxidants in tea have health benefits.
Used in which kind of disorders & write the amount of
fortified element.
Under IDDCP, which type of sample is taken to identify the
“proportion of population consuming iodized salt.
Daily requirement of iodine.
Iodized Salt.
• Iodine deficiency disorders.
• 15ppm at the consumer level & 30ppm at
manufacturing level.
• Urine iodine excretion.
• 150 micrograms for adults & 250 micrograms for
pregnant women.
What is sodium intake in hypertension.
Classify blood pressure(JNC-VIII)
What is DASH strategy.
Salt: Not more than 5gms. Per day.(2.4 gm of sodium).
JNC VIII: Systolic Diastolic
Normal- 100-119 60-79
Prehypertension- 120-139 80-89
Hypertension I- 140-159 90-99
Hypertension II >160 >100
DASH: (Over all goal)
Maximize the opportunities for primary prevention of
HIV/STD and pregnancy among teens .
What is the use of given spot.
What should be the level of fortified element in test
sample at manufacturing and consumer level.
Name the indicator/ agent used in given spot.
If testing kit is not available how to know iodine in salt
at domestic level.
• Iodine Testing Kit
• To measure the level of iodine in salt.
• 15ppm at the consumer level & 30ppm at
manufacturing level.
• Indicator in iodine kit is starch & iodine.
Write the dosage schedule.
Mention the Ocular & extra-ocular sign & symptoms of Vitamin A deficiency .
What are VAD criteria.(WHO)
Vitamin A:
• 1 lac IU at the age of 9 months & than 2 lac IU at the age of 16 months,
than after every 6 months up to 5 years(total 9 doses), solution given
orally
• Night blindness,conjuctival Xerosis , Bitot’s spots, corneal Xerosis,
Keratomalcia.
• Increase in infections(respiratory & intestinal)
• Follicular hyperkeratosis, anorexia & growth retardation.
• Daily requirement for adult man & woman-600mcg,pregnant-800mcg
& lactating-950mcg.
• VAD criteria –night blindness->1%,bitot”s spots->0.5%, corneal xerosis-
>0.01%, corneal ulcer->0.05% & serum retinol less than 10mcg/dl->5%.
Mention the chemical composition of adult tablet.
How many tablets are given to a female during pregnancy under
national program & rationale for its use in pregnancy in India.
Writ down the cut off point for Hb for diagnosing anemia in
pregnant female.
How will you evaluate iron status.
Mention two common side effects of given spot.
Iron & Folic acid Tablet:
Dose- 100mg of elemental iron & 0.5 mg of folic acid.
One tablet a day, for 100 days during pregnancy.
Less than 11g/dl.
Hb concentration, serum iron concentration(,50 mg/L),serum ferritin(,10mcg/L) &
serum transferrin saturation(,15%).
Daily requirement for adult male is 17mg,female & lactating mother-21mg,pregnant
woman 35mg.
Constipation & diarrhea.
Nausea & vomiting.
Stomach upset & heart burn
Mention two advantages & disadvantages of
contraceptive.
What is its failure rate.
Condom: (Thin latex rubber device)
• Prevents the semen being deposited in vagina
• Easily available
• Safe & inexpensive.
• Easy to use & does not require medical supervision.
• No side effects.
• Light , compact & disposable.
• Provide protection against STD.
• 2-3 per 100 women years of exposure to more than 14 in typical
users.
• Disadvantages-may slip off or tear, interferes with sex.
• Spermicide jelly may be used with it to reduce the failure
Write the mechanism of action of the contraceptive.
What are its absolute contraindications.
What is its failure rate.
What are the timing of its insertion.
Disadvantages.
Copper-T:
• Foreign body reaction in uterus causing cellular & biochemical changes
in endometrium & uterine fluids, impairing viability of gamete-reducing
chances of fertilization. Copper ion also changes biochemical
composition of cervical mucosa-affecting sperm motility.
• Suspected pregnancy, PID,Cancer of cervix,vaginal bleeding, Previous
topic pregnancy.
• 0.5-0.8 per cent.
• Within 10 days of beginning of menstruation period, during first
week after delivery, or 6-8 weeks after delivery.
• Requirement of trained person for insertion and removal
• Need to follow up for initial few months
Write the failure rate of the contraceptive.
Mention non-contraceptive health benefits.
Mention chemical composition.
Write the mechanism of action.
Mention absolute contraindications
Oral pills:
• Less than one percent.
• Protection against diseases like benign breast disorders, ovarian
cysts,PID, ectopic pregnancy, iron-deficiency anemia, ovarian cancer.
• Levongestrel 0.15 mg& ethinyl estriodiol0.03 mg.
• To prevent the release of ovum from ovary by blocking the pituitary
secretion of gonadotropin.
• Cancer of breast & genitals, Liver disease, previous or present history of
undiagnosed abnormal uterine bleeding, thromboembolism, cardiac
abnormalities, congenital hyperlipidemias, und
Write down two indications for which it should be
used.
Mention the time period during it should be taken
• Emergency pill(Levnorgestrel 0.75mg) is approved for
emergency contraception.
• It should be used within 72 hours of unprotected sex,rape
or contraceptive failure
• One tablet should be given within 72 hours of unprotected
sex & second tablet is given 12 hours after that.
• Failure rate is less than 1%.
• Disturbs ovum release, fertilization & implantation.
• Side effects are lower abdominal pain, nausea, vomiting,
tenderness in breast, headache, irregular menstrual
bleeding.
Mention the use of apparatus.
Mention the name of the test to find out free & combined
chlorine.
Mention the indicator used while using the given spot?
Describe how to prepare that indicator solution?
What is the “stock (mother) solution” with reference to given
spot, and describe how to prepare the same?
Horrock's apparatus.
• To find out the demand of bleaching powder to disinfect the
water.
• Orthotoludine test(OT).
• Starch Iodide.( 1.5 gm of starch is added to 100ml of distilled
water, boiled & cool, 7.5 gm of potassium iodide is added.
• 2gm of bleaching powder in black cup, make a paste, add
more water up to the mark(Stock solution)
Mention the name of indicator used in this apparatus
Calculate the amount of bleaching powder for a well having
4550 liters of water, with horrock’s apparatus showing blue
color in third cup
Horrock’s apparatus.
• Starch iodide.
• 3x2 = 6 g are required for 455 liters.
• 6/455 x 4550 = 60 g.
Identify the equipment.
Mention its uses
Vaccine Carrier
• To transport vaccine in small quantities(16-20)
vials for the outreach sessions
• Temperature is maintained at 4-8 degree C.
• 4 fully frozen ice packs are used for lining the
sides
• Vials of DPT,DT & TT & diluents should not be
placed in direct contact with frozen ice packs.
Identify the equipment
Mention its use
What is “conditioning” with reference to given spot?
How do you ensure that conditioning is complete?
Mention advantage of conditioning.
ILR
– ILR, at PHC level, because electricity supply at PHC level is
not reliable. ILR can maintain the required temperature
(2-8 degree C) for 48 hours in absence of electricity.
– “Hold over time” of cold chain equipment is the time
taken by equipment to raise the inside cabinet
temperature without electricity from its temperature at
the time of cut to maximum temperature of its
recommended range, provided that equipment is
maintained & functioning well. In case of ILR cabinet
temperature is +4deg. C, time taken to reach 8deg.C will
be hold over time.“Hold over time of ILR = 48 Hours.
Write the dose, route of administration& its schedule.
Name the diluent used for its reconstitution.
Mention adverse affects of this vaccine
Mention the strain recommended for production of given spot.
Discuss the phenomenon after BCG vaccination.
BCG Vaccine:
• Dose-o.1ml, less than 4 weeks-0.05ml.
• Intradermal at the insertion of deltoid.
• At birth or as early as possible till one year.
• Diluent is normal saline.
• Prolonged severe ulceration at the site,suppurative
lymphadenitis,osteomylitis,,disseminated BCG infection & death.
• Contraindications are-patient with generalized eczema, Infective dermatosis, to
those with a history of deficient immunity, treatment on steroids or other
immunosuppressant drugs.
• “Danish1331” strain recommended by WHO.
• Two to three weeks after the vaccination, papule develop at the site which
increase in size in about 5 weeks(4-8 mm),breaks in to a shallow ulcer, healing
Mention the dose, route & schedule of the vaccine.
Comment on the stage of VVM of the vaccine
DT vaccine:
• 100% against diphtheria & tetanus.
• Shake test.
Mention the dose, route & schedule.
Tetanus toxoid:
• 0.5 ml, intramuscular,, upper arm.
• TT-1- Early in pregnancy.
• TT-2- 4 weeks after the TT-1,
• TT-Booster-If received2 TT doses in a pregnancy
within last 3 years.
• In children at the age of 10 & 15 years.
• Contraindications are H/o previous allergy, acute
& severe infections
Mention the dose, route & schedule
JE Vaccine:
• Inactivated(Nakayama strain)
• Dose-3 dose, first on selected day, second on 7th &
third on 30th day.(0.5 ml for 1-3 year of age & 1ml
for>3 years, subcutaneous, upper arm)
• Booster-before one year of primary & every 3 years.
• Powdered vaccine with diluent.
• Protective value about 3 years.
Mention the dose, route & schedule
Rotavirus Vaccine:
• Tetravalent(4 serotypes G1 to G4)
• Primary-3 doses at 2,4 & 6 months.
• Protective value -80%.
Round Worm
Name the helminth.
What is the infective stage of given spot.
What is the treatment of given spot.
Ascaris.
– It infects humans when an ingested fertilised egg becomes a larval worm
(called rhabditiform larva) that penetrates the wall of the duodenum and
enters the blood stream.
– Albendazole 400 mg stat , Albendazole is not recommended during
pregnancy; pyrantel pamoate is the drug of choice in these
cases.Alternative therapy is mebendazole (100 mg bid for 3 d or 500 mg
as a single dose). Mebendazole is not recommended during pregnancy;
pyrantel pamoate is the drug of choice in these cases.
• Primary: Sanitary disposal of human excreta,, provision of safe
drinking water, use of sanitary latrine by community.
• Secondary prevention: Albendazole, dose for adult & children
above 2 years is 400 mg as a single dose.
Mebendazole: 100 mg twice a day for 3 days for all ages above 3
years.
Hook Worm
Name the helminth.
What is the drug of choice for its treatment.
Which index is used for epidemiological study of the given spot? Describe the interpretation of same.
Hook worm.
• Causes chronic blood loss
• Prevention & control:
• Sanitary disposal of faeces, Chemotherapy
(Albendazole or Mebndazole), correction of anaemia
& health education.
Rat Flea
Name the disease transmitted by this organism.
Write two control measures used against this
organism
Rat flea(Dark brown in color, bilaterally compressed wingless
body, head is conical attached directly to thorax)
• Plague by bite of blocked flea, endmic typhus by
contamination of skin with faces of flea
• Insecticides-DDT & BHC should be used as dusts containing 10%
& 3% of the active ingredient. If resistant to both malathion 5%
is used.
• Rat burrows should be insufflated with insecticidal dust with the
help of dust blower.
• Flea index is the measurement of density of fleas per rat, kept
below 1, useful in evaluation of control measures & forecast
Anopheles(Female)
Identify the organism with identification feature. & the
stage of life cycle.
Name two insecticides with their mechanism of action
used against it.
Name the disease transmitted by it
• Adult anopheles.
• Proboscis & palpi are equal in length.
• Wings are spotted, antennae are not hairy.
• DDT, BHC, Malathion.
• Contact poison.
• Personal protection.
• Larvicidal measures(Gambusa fish)
• Malaria(plasmodium species)
Culex(Female)
Identify the organism& identification features.
Name two diseases transmitted by it.
Name two insecticides with their mechanism of action
used against it.
Genetic control measures.
– Culex (Adult)
– 3 compartment body – head, thorax, and abdomen. 3 pair legs and 1
pair wings. Unspotted wings, no white stripes on body and legs, palpi
shorter proboscis.
– C. fatigans, C. vishnui, C.tritaeniorhynchus
• Filariasis- Wuchereria bancrofti, Japanese encephalitis-
Arboviruses.
• DDT, BHC, Malathion.
• Contact poison.
• Genetic control-Sterile male technique,cytoplamic
Aedes
Identify the organism with identification features.
Mention methods used for control of breeding places
of mosquitos
Name the disease transmitted by it
Streptomycin
– Previously treated TB patients, route I.M.
– Protein synthesis inhibitor. It binds to the small 16S rRNA
of the 30S subunit of the bacterial ribosome, interfering
with the binding of formyl-methionyl-tRNA to the 30S
subunit.
• Adverse effect:- nephrotoxicity, ototoxicity
Write the composition of new WHO approved ORS.
Write the uses of ORS & Mechanism of action.
What is the importance of osmolality of solution prepared using the
given spot?
What is “super ORS”? & its advantages.
• For one liter of water(Helps in hydration)
• Glucose-13.5g(Glucose-75mEq/L)promotes sodium & water absorption)
• NaCl-2.6g(Sodium-75mEq/L, chloride-65mEq/L) (prevents hypernatremia)
• KCl-1.5g(Potassium-20mEq/L, Chloride-65mEq/L) (Prevents hypokalemia)
• Trisodium Citerate-2.9g( 10mEq/L,Increase absorption of sodium &water, prevents
acidosis)(Total-245mmol.)
• To treat dehydration& maintaining rehydration.
• Reducing osmolarity avoids adverse effects of hypertonicity on net fluid absorption.
• Super-ORS contain complex sugar instead of monosugar.It may be food based(rice
based) or starch free(glycine/alanine based)
• Provide rehydration.
• Reduce stool out put,frequency & duration.
• Increased nutrition-180Kcal/liter.
• With gradual release of glucose-prevent secondary diasaccharide intolerance.
• Contribute to weight gain.
Mention the use of given spot in public health.
Write its dose
Write the mechanism of action.
Alum( Aluminium sulphate, white in color)
• Used to remove turbidity of water before subjecting to
rapid sand filtration.
• 5-40 mg/liter, depending upon turbidity, color,
temperature & pH value of water.
• Chemical coagulant.
• Helps in sedimentation(settles down the impurities &
bacteria in water) by neutralizing the electronic double
layer surrounding the fine particles & allowing them to
flocculate.
Mention the concentration of chlorine in fresh
bleaching powder
Write its mechanism of action
Mention its uses.
How it can be used continuously.
• Bleaching Powder(White powder with pungent smell of
chlorine)
• 33% of available chlorine.
• Rapidly loses its chlorine content on exposure to air, light &
moisture-hence stored in dark, cool & dry place)
• Germicidal effect is by hypochlorous acid, nascent oxygen
& chlorine.
• Disinfection of water, feces, urine & pus.
• By using “double pot” method.
Write mechanism of action of given insecticide.
Write dosage for its use in form of “IRS” against
mosquitoes
Malathion(Yellow or clear brown liquid with
unpleasant smell.)
– Organophosphorous insecticide act as Contact poison.
– Indoor Residual spray = 100-200 mg / square feet every 3
months.
– Because of its low toxicity, it has been recommended as
an alternative insecticide to DDT.
– As a low volume spray, widely used for killing
mosquitoes tom prevent DHF.
Write its uses.
List two demerits of its usage for purification of
drinking water
• Benzene hexachloride(BHC/Gammexane)
• White colored powder with musty smell.
• Insecticide, acts as contact poision.
• Gamma isomer is active component.
• Residual effect for 3 months.
• 20-50mg per square foot of spraying area.
Write its use in public health.
Active components of the spot are?
What is the dose?
• Dissecting microscope.
• It is designed for low magnification observation of a sample
using light reflected from the surface of object rather than
transmitted through it.
• It is used with a dye on the slide to view tissue samples,
blood, microorganisms.
• Each eye have one objective so that 3D objects can be seen.
It has low magnification(10X to 40X) with higher resolution.
(i) Identify the instrument.
• Sling Psychrometer:
• Used to find out RH.
• Have 2 mercury thermometers(wet & dry), mounted side by side
on a wooden frame, provided with a handle for rotating the
instrument.
• Wet bulb is moistened & than instrument is rotated for about 15
seconds at the rate of 4 revolutions per second to obtain the
desirable speed of 5 meters/second.
• Reading of wet bulb is taken, again rotated, second reading is
taken,repeated till 2 readings are same.
• Now take the reading of dry thermometer.
• By using suitable charts, RH of the air is obtained.
(i) Identify the instrument.
(ii) Write its use in Public Health.
• Clinical thermometer
• To measure the human body temperature.
• Usually it is mercury thermometer, it is accurate & sensitive
because it has a narrow space where mercury level rises very
fast.
• We use maximum thermometer, temperature reached is
maintained even after it is removed from the body.
•
What is measured by given spot? Write cut-off values for the
interpretation of measurements by same.
Which age group assessed by using given spot?
Shakir tape
Chloroscope
It used to measure free and combined chlorine content in
water.
Used in water treatment plants,industrial concerns,
community water supply schemes, railway,defence & state
govt. departments.
Reagent used= Orthotolidine dissolved in 10% HCL
(hydrochloric acid).
Recommended level of free residual chlorine is 0.5 mg/L
(i) What is the role of this spot in National
Immunisation Schedule (NIS, India)?
(ii) What is the cold chain temperature at which it
should be stored?
• Sterile water
• Used as diluent for BCG vaccination.
• +2 to + 8 deg.C.
(i) Identify the given spot.
(ii) Write it’s TWO uses in Public Health.
• Tuberculin syringe.
• Omega microstat syringe fitted with a 1 cm steel 26 gauge
intradermal needle.
• Used for doing tuberculin test.
• Used foe giving BCG vaccination.
(i) Identify the given spot.
(ii) Write it’s use in Public Health
• N95 mask
• It is respiratory protective device designed to fit the face & does very efficient
filtration of airborne particles.
• N-95 filter means 95% of particles>(0.3 microns) are filtered by it.(Respirable
dust is 0.5-3 micron in size)
• It is required to prevent the transmission of Measles, SARS, Varicella, TB etc.
• It can also protects from wild fire smoke.
• Blue side should be inside & white outside if you want to protect yourself &
vice versa if you want to protect others from you.
• Ergonomics is fitting the job to the worker.
• X-ray in silicosis show snow storm appearance due to nodular fibrosis in upper
part of lungs, while in asbestosis it is ground glass appearnace in lower 2/3 of
lungs due to diffuse fibrosis.
Identify the spot.
Write its mechanism of action.
Write its use.
Autoclave:
• It attains the temperature of 122 deg.C under
15 lbs/sq. inch pressure.
• It acts by giving off latent heat.
• Used in hospitals & labs.
• It destroys all forms of life including spores.
• It is the most effective method for sterilization
of linen, dressings,gloves, syringes & culture
media.
Identify the spot.
Write its use in public health.
• Berkefeld Filter:
• It is a ceramic filter for purification of water on
small scale .
• Essential part of the filter is candle made of
infusorial earth.
• Filter candles are liable to be logged with impurities
& bacteria.
• Should be cleaned by scrubbing with head brush
under running water & boiled at least once a week.
• Only clean water should be used .
Write type / name of items for which the given spot is used
according to latest rules.
Write treatment/ disposal options for the same
Cigarette
– Third hand smoke= Residual nicotine and other chemicals of tobacco smoke, left on
indoor surfaces (e.g. clothes, curtains etc). people are exposed to these chemicals
by touching contaminated surfaces or breathing in off-gassing from these surfaces.
– Cigarettes and other tobacco products (Prohibition of Advertisement and
Regulation of trade and commerce, production, supply and distribution) Act, 2003.
– No Tobacco Day on 31st May every year(tobacco & heart disease-2018)
– To expand the fight against the tobacco epidemic, WHO has introduced the
MPOWER package of six proven policies:
– • Monitor tobacco use and prevention policies,
– • Protect people from tobacco smoke,
– • Offer help to quit tobacco use,
– • Warn about the dangers of tobacco,
– • Enforce bans on tobacco advertising, promotion and sponsorship, and • Raise
taxes on tobacco.
– The MPOWER policy package can reverse the tobacco epidemic and prevent
millions of tobacco related deaths.
Identify the equipment.
Mention its use
• Herpender’s caliper.
• To measure skin fold thickness or body fat
percentage.
• Pinch the fold of skin away from muscles &
measure with instrument & match the reading with
chart to find out body fat percentage.
• Normal body fat percentage for young male is 8-
19% & for female is 21-33%.
Identify the equipment & write its use
• Sterilizer.
• To destroy the microorganisms by obtaining high
temperature with steam, dry heat or boiling liquid.
• Used to sterilize the instruments.
Identify the spot.
Write its principle of working.
Write its use in public health.
Centrifuge Ramix:
• Works on the principle of sedimentation, where centripetal
acceleration is used to separate the substances of different
densities.
• Used for biochemical analysis of body fluids,like blood cells
from serum.
• Used in molecular biology to separate cellular components
& DNA fragments.
• In Public Health Labs. For water chemistry analysis.
Identify the spot.
Write its use in public health.
• Fortin Barometer:
• It is mercury based.
• Used in industrial labs, Public Health Labs.
Meteorological departments to measure the
atmospheric pressure.
• At high altitudes, atmospheric pressure decreases,
causing motion sickness(headache, insomnia, nausea,
vomiting, pulmonary edema.
• Below sea level atmospheric pressure increases, leading
to Caisson’s disease( High level of dissolved nitrogen in
blood) – air embolism, convulsions & death.
Identify the spot.
Write its use in public health.
• Infantometer:
• To measure the length of newly born & infants
in lying down position.
• It is used to monitor the growth of the child-
height for age.
Identify the spot.
Write its use in public health.
What is the optimal temperature?
• Incubator:
• Lab. Incubator is used to grow & maintain
microbiological & cell cultures.
• It is a insulated device with adjustable heater to
maintain temperature, humidity & other conditions
like amount of gases (Co2 & O2) inside.
• To grow most of the bacteria & mammalian cells
optimal temperature of 37deg. C is required.
Identify the instrument.
Write its uses in public health.
• Spirometer:
• Used to measure the volume & rate of air inspired & expired
by the lungs over a specified time.
• Output produced by spirometer is called kymograph, from this
vital capacity, tidal volume, breathing rate & ventilation rate
are calculated.
• Used for screening of high risk individuals(smokers)
• Measuring the effect of disease on pulmonary functions.
• Pre-operative risk assessment.
• Assessment of therapeutic interventions(bronchodilators)
• In public health, used for epidemiological surveys &
Identify the spot.
Write its use in public health.
• Lactometer(Hydrometer):
• It is a device to measure cream content by
measuring specific gravity of milk.
• Thus it tells purity & richness of milk.
• Measurement should be taken when lactometer is
floating freely in milk.
• It helps to check water adulteration of milk.
Identify the spot.
Write its use in public health.