EXTRACTION OF NATURAL DYES
FROM FLOWER AND USED IT
AS ART PAINT”
Name:- Abhishek Sabale
(MUBBT202401) Abhishek
Gayke(MUBBT202402)
Project Guide :- Ms.Meena Chittapure
INTRODUCTION
There is a growing demand for eco-friendly/non-toxic colorants, specifically for health sensitive applications
such as coloration of food and dyeing of child textile/leather garments. Recently, dyes derived from natural
sources for these applications have emerged as an important alternative to potentially harmful synthetic dyes
and pose need for suitable effective extraction methodologies. The present paper focus on the influence of
process parameters for ultrasound assisted leaching of coloring matter from plant materials. In the present
work, extraction of natural dye from beetroot using ultrasound has been studied and compared with
static/magnetic stirring as a control process at 45 °C. The influence of process parameters on the extraction
efficiency such as ultrasonic output power, time, pulse mode, effect of solvent system and amount of beetroot
has been studied. The use of ultrasound is found to have significant improvement in the extraction efficiency of
colorant obtained from beetroot. Based on the experiments it has been found that a mixture of 1:1 ethanol–
water with 80 W ultrasonic power for 3 h contact time provided better yield and extraction efficiency. Pulse
mode operation may be useful in reducing electrical energy consumption in the extraction process. The effect of
the amount of beetroot used in relation to extraction efficiency has also been studied. Two-stage extraction has
been studied and found to be beneficial for improving the yield for higher amounts of beetroot. A significant 8%
enhancement in % yield of colorant has been achieved with ultrasound, 80 W as compared to the MS process
OBJECTIVES
The present investigation entitled “EXTRACTION OF NATURAL DYES FROM FLOWER AND
USED IT AS ART PAINT” will be conducted with the following objectives.
Collection of samples.
Extraction of pigment.
Characterization of Dye.
REVIEW OF LITRATURE
Samanta & Agarwal, [2009]. The flowers, which contain much of tannin, are Flame colored and yield red/pink/brown/flame-colored shades of
dye (depending upon the fabric used) in large amounts, therefore, utilized throughout India for dying silk and fabrics on a commercial scale. Light
fastness of many natural dyes, particularly which are extracted from flower parts are found to be poor to medium. India was a major exporter of
herbal dyes but not so recently because of the ban on production of some of the synthetic dyes and intermediates in the developed countries due to
pollution.
Kumaresan, Palanisamy & Kumar, [2011]. The plant possesses many medicinal properties. Flowers are the most effective fermentation agent,
used in Ayurvedic medicines (Kroes et al, 1990). Many investigations revealed that the use of a combination of mordants in varying ratios gives
different shades and different color fastness results.
Pizzicato et al. (2023), The dyeing and finishing step represent a clear hotspot in the textile supply chain as the wet processing stages require significant
amounts of water, energy, and chemicals. In orderto tackle environmental issues, natural dyes are gaining attention from researchers as more sustainable
alternatives to synthetic ones. This review discusses the topic of natural dyes, providing a description oftheir main features and differences compared to
synthetic dyes, and encompasses a summary of recent research in the field of natural dyes with specific reference to the following areas of sustainable
innovation: extraction techniques, the preparation of substrates, the mordanting process, and the dyeingprocess. The literature review showed that promising
new technologies and techniques have been successfully employed to improve the performance and sustainability of natural dyeing processes, but several
limitations such as the poor fastness properties of natural dyes, their low affinity with textile substrates, difficulties in the reproducibility of shades, as well
as other factors such as cost-effectivenessconsiderations, still prevent industry from adopting natural dyes on a larger scale and will require furtherresearch
in order to expand their use beyond niche applications.
METHODOLOGY
Collection of Sample
Aqueous-Ethanol extraction
method
Soxhlet extraction
method
Thin-Layer Chromatography
Phytochemical Test:
Test for Alkaloids:
Mayer’s Test
Wagner’s Test
Test for Carbohydrates:
Benedict’s Test
Test for phenolic Compound:
Ferric Chloride Test
Test for protein:
Million’s Test
RESULT
Ethanol extraction method:
a) The Transvaal Daisy and Lavender were crushed in 70% Ethanol.
b) The Flask was incubated over a water bath for 4Hr at 68o C.
c) The Solution was left overnight in a hot air oven to dye extract.
Soxhlet Extraction Method:
•This method was carried out to filter the solvent and residue, and also to obtain the separation.
•The organic solvent ethanol was used for this extraction method.
•In this method the weight quantity of crushed flowers and measured amount of solvent were taken in a certain
ratio.
•The raw material (Sample) was kept in a thimble of Soxhlet extraction the solvent
was poured into a round bottom and a condenser with a high flow rate of water was fitted over it.
•The extraction was carried out for 4-5 hrs. and the volume of the solution obtained was measured.
Thin-Layer Chromatography:
By using Acetone and Hexane as solvent systems the Rf value of The dye
was : By using ethanol as solvent system the Rf value of the dye was:
As per Thin-layer chromatography, we confirm that the dye contains
Xanthophyll pigment
By using Acetone and Hexane as solvent systems the Rf value of The dye
was :
Sr No Rf Value
1 0.78
2 0.85
3 0.95
By using ethanol as solvent system the Rf value of the dye
was:
Sr No Rf Value
1 0
2 0.86
3 0.60
LIST OF FIGURE
Crushing of flowers Dye Extract
Thin layer chromatography of Millon’s test Extraction of dye using Soxhlet
dye method
Mayer’s Benedict’s test
test
Wegner’s test Ferric Chloride test
CONCLUSION
The current work indicated that natural dye may be successfully extracted from the petals of
Transvaal daisy and Lavender The outcome indicate that there is about 1.4–32.4%improvement in
% yield of extract obtained as a result of usage of ultrasound when compared with magnetic
stirring at 45 ◦C. This technique provides effective use of natural resources as eco-accommodating
technique in current circumstance of worldwide natural concern. From this current study it was
found that the natural dye extracted from the two different plant source extracts can be
successfully applied to the cotton fabrics to obtain a wide range of colour shadings along with the
application of the mordant as a fixative agent. These dyes are environmentally friendly and
harmless when compared to the synthetic dyes.
FUTURE LINE OF SCOPE
Research into new natural dyes sources along with eco-friendly, robust, and cost-effective
technologies for their processing and application have greatly aided in widening the scope of natural
dyes in various traditional and advanced application discipline. The dyes industry in India is
expected to witness a steady growth in the coming years due to environmental crackdowns in China,
resulting in a shutdown of several domestic dye companies. India is better placed due to the
availability of the ecosystem, feedstock, technology, and compliance required for the industry.
REFERENCES
Burrows, V. (2004) . Experiments and other learning activites using natural dye materials Chemical
Engineering Education, 38 (2).
Sivakumar, V., Vijaeeswari, J., & Anna, J. L. (2011). Effective natural dye extraction from different plant
materials
using ultrasound. Industrial Crops and Products, 33(1), 116-122.
Zhou, H., Wu, L., Gao, Y., & Ma, T. (2011). Dye-sensitized solar cells using 20 natural dyes as sensitizers.
Journal of
Photochemistry and Photobiology A-chemistry, 219(2–3), 188–194.
Shahid, M., Shahid-Ul-Islam, & Mohammad, F. (2013). Recent advancements in naturaldye applications: a
review.
Journal of Cleaner Production, 53, 310–331
THANK YO U
