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UV Microscope: Principles & Benefits

The document summarizes the key components and working principle of an ultraviolet microscope. It discusses the light source, objective lens, eyepiece or camera, specimen stage, filters, and other optical components. The UV microscope uses UV light to illuminate specimens, which are then magnified and captured as images. This allows for enhanced resolution, fluorescence imaging, and applications in biology, research, and industry by taking advantage of light-matter interactions in the UV spectrum. However, limitations include restricted sample compatibility, safety concerns, and potential damage to sensitive specimens.

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1K views13 pages

UV Microscope: Principles & Benefits

The document summarizes the key components and working principle of an ultraviolet microscope. It discusses the light source, objective lens, eyepiece or camera, specimen stage, filters, and other optical components. The UV microscope uses UV light to illuminate specimens, which are then magnified and captured as images. This allows for enhanced resolution, fluorescence imaging, and applications in biology, research, and industry by taking advantage of light-matter interactions in the UV spectrum. However, limitations include restricted sample compatibility, safety concerns, and potential damage to sensitive specimens.

Uploaded by

maduraiagrians
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

Ultraviolet

Microscope
Presented by
Navina KR
2022002116
microscop
e
Topics • Working
covered: principle
• Advantag
2
Introduction
• Optical microscope equipped
with uv light sources and
detectors.
• Imaging and analyzing
specimens using UV light
• 3
Parts of a UV microscope
• Light source
Such as mercury or uv led
To provide necessary uv illumination
for the specimen
• Objective lens
Collects and magnifies the uv light that 4
• Eyepiece or camera
For capturing images and videos of the
uv illuminated specimen.
• Specimen stage
Holds the sample under the objective lens
and Presentation Title 5
• Filters
To control the wavelength of uv light &
eliminate unwanted wavelength
Enhance contrast and improve image quality
• Optical system
Consists of various lens and mirrors to direct
Presentation Title 6
• Detector
Capture the uv light that has interacted with
the specimen
These can be sensitive cameras /
photomultiplier tubes
• Focusing mechanism Presentation Title 7
• Illumination control
Uv microscopes may have control for adjusting the
intensity and duration of uv light exposure
• Stage control
Knobs or controls for precise movement of the
specimen stage in X, Y and Z axes
• Eyepiece diopter adjustment.
Presentation Title 8
Working principle
• Uv light source
• Filter
• Specimen
Absorption
Fluorescence
• Objective lens
• Eyepiece
9
Advantages
1. Enhanced 6. Biological applications
resolution 7. Filtered excitation
8. Research and forensics
2. Fluorescence
9. Semiconductor industry
imaging
3. Reduced scattering
Presentation Title 10
Limitations
1. Limited sample Optical abberations
compatibility Limited
2. Safety concerns fluorophores
3. Reduced depth of 4. Sample damage
field Presentation Title 11
Reference
1. Introduction to light microscopy by Peter. B. Moens,
Joseph A Zeiss and Mark De Boer
2. Handbook of biological confocal microscopy edited
by James B Pawley
3. https://www.britannica.com
4. https://www.microspecrta.com
Presentation Title 12
Thank you

13

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