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Specific Laboratory Induction

The document provides an overview of laboratory hazards and safety procedures at KU Environment, Health & Safety Management System. It discusses various physical, chemical, biological, and mechanical hazards found in laboratories. It also outlines personal protective equipment requirements, safe work practices including prohibitions on eating or smoking in labs, and emergency response procedures for incidents such as chemical spills, fires, or injuries. Laboratory security, signage, and waste disposal procedures are also summarized.

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Mohamed Aslam
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109 views36 pages

Specific Laboratory Induction

The document provides an overview of laboratory hazards and safety procedures at KU Environment, Health & Safety Management System. It discusses various physical, chemical, biological, and mechanical hazards found in laboratories. It also outlines personal protective equipment requirements, safe work practices including prohibitions on eating or smoking in labs, and emergency response procedures for incidents such as chemical spills, fires, or injuries. Laboratory security, signage, and waste disposal procedures are also summarized.

Uploaded by

Mohamed Aslam
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

KU Environment, Health & Safety

Management System
‘Getting to Know Your University EHSMS’
Specific Laboratory Induction
1. Laboratory Hazards

Beginning with

the end
2. Safe Conduct & Good Laboratory Practices

in mind 3. Personal Protective Equipment & Safety Equipment


CLASSIFICATION OF LABORATORY HAZARDS

 Physical:

Lighting, Electricity, Heat, Noise, Ionising Radiation, Thermal Stress

 Chemical:

Acids, Alkalis, Fumes, Gases, Dust particles, toxic substances

 Biological:

HIV Virus, Legionella Bacteria, organisms

 Mechanical

Equipment, Machinery, Tools, Conveyers

 Radiation
Ionizing, Non-Ionizing, Radioactive materials
LABORATORY DOOR SIGN

 A warning sign is posted at the


door of each laboratory to provide
an indication of the possible
hazards in that laboratory and the
appropriate personal protective
equipment required.
 The warning sign also includes a
list of emergency contact persons.
In the event of an accident,
chemical spills or fire, assistance
from the people on that list may be
requested.
LABORATORY SECURITY

Access to all laboratory facilities


is restricted to authorized
personnel specified by the
laboratory supervisor
NOT PERMITTED IN LABORATORY

 Eating
 Drinking
 Smoking
 Handling Contact Lenses
 Storing Food and Drinks
 Chewing Gum
 Applying Cosmetics
 Inserting Fingers, Pens or
Other Articles in the Mouth
 Pipetting by Mouth
BEFORE LABORATORY WORK, GET TO KNOW

 Hazards of materials & agents


and their prescribed safety
procedures.
 Safe code of conduct.
 Designated escape route and
alternate.
 Location of fire extinguishers,
eyewash and shower station, fire
blankets, first aid, and spill kits.
 Emergency telephone numbers.
FIRST AID

The KU provides a first aid service to all areas

GET MEDICAL TREATMENT HOWEVER SMALL THE INJURY


 First aid boxes are provided in all KU Laboratories and are the
responsibility of the Lab Engineer.
 First aid box is situated near the door of each Lab, and is accessible to all.
STANDARD BEHAVIOR FOR ALL PERSONNEL ENTERING A
LABORATORY

 Know the types of and the use of personal protective equipment available for your
laboratory operation.
 When entering the laboratory, wear laboratory coats. Sandals and open toed shoes
should not be worn in the laboratory at any time.

 Long hair and loose clothing should be confined when in the laboratory. Make sure it is
tied back or confined under your laboratory coat.

 Do not keep your hand bags and belongings on the workbench of


laboratory equipment while working.
WHILE WORKING IN THE LABORATORY

 Use appropriate eye, skin, and hand


protection.
 Wear appropriate PPE e.g. lab coat
or gown in the proper manner.
 Wear gloves in the proper manner.
 Wear a face shield or safety goggle
in the proper manner.
 Wear appropriate respiratory mask
for the work being conducted.
 Take off your Lab coat before exiting
the lab.
WHILE WORKING IN THE LABORATORY

 Wear shoes that enclose your entire feet


(with heels not more than1¼'' in height.
 Tie long hair neatly at the back of the
neck (secure hair/beard).
 Restrain loose clothing.
 Remove jewelry when working with
chemicals, biohazards, radioactive
material.
 Covering any cuts, open sores or wounds.
 Follow universal precautions.
 Handle unknowns as if they were
hazardous.
BEFORE YOU LEAVE THE LABORATORY

 Decontaminate your workbench with


disinfectant at the end of the duty.
 Leave all equipment, samples and
reagents in the lab.
 Return unused equipment, apparatus,
etc.
 Wash your hands with antiseptic soap
and water.
WHILE WORKING IN THE LABORATORY

 Handle volatiles in a
chemical fume hood.

 Contain bio aerosols in a


biological safety cabinet.
WASTE MANAGMENT

 Hazardous waste:
o Chemical
o Biological
o Radiation

 Recyclable waste.
 General waste.
BROKEN GLASS BOTTLES

 Non Broken Glass: Bottles waste don't put in

medical lab waste boxes. Non broken glass shall

be removed by custodians.

 Broken Glass: if clean, should be disposed of in a

“Broken Glass” box.


SHARPS DISPOSAL

 Segregate from other wastes.


 Place in rigid plastic boxes with
tightfitting lids.
 Write date and location.
 Fill container only 3/4 full.
 Disinfecting not needed (but required
for BL3 labs).
 No sharps shall be disposed in trash
container or waste bags.
NEEDLE STICK AND SHARPS INJURIES

Risks due to Sharps Injury from a known


positive source
Sharps injuries can expose workers to a
number of bloodborne pathogens that can
cause serious or fatal infections. The
pathogens that pose the most serious health
risks are
 Hepatitis B virus (HBV)
 Hepatitis C virus (HCV)
 Human immunodeficiency virus (HIV) the
virus that causes AIDS
RED OR YELLOW PLASTIC SHARPS CONTAINERS

Red or yellow (hard plastic) sharps containers contain

needles, glass pipettes, and Pasteur pipettes, microscope

slides, cover slips, blades, scalpel blades, etc.


SAFETY SIGNS AND MARKING SYSTEM
LABELING AND MARKING SYSTEMS
MSDS/ SDS

Cheminfo is an online
database providing
information about chemicals
through a series of Material
Safety Data Sheets (MSDS).
MATERIAL SAFETY DATA SHEETS

1. Product Information 9. Physical and Chemical


2. Composition, Ingredients Properties
3. Hazards Identification 10. Stability and Reactivity
4. First Aid Measures 11. Toxicological Information
5. Fire Fighting Measures 12. Ecological Information
6. Accidental Release 13. Disposal Considerations
Measures 14. Transportation Information
7. Handling and Storage 15. Regulatory Information
8. Exposures Controls, 16. Other Information
Personal Protection
CATEGORIES OF HAZARDOUS CHEMICALS

Physical Hazards
o Flammables and Combustibles
o Oxidizers
o Reactive
o Compressed Gases
Health Hazards
o Corrosives
o Toxins (poisons)
TARGET ORGANS

Nasal passages - Nickel and chromium compounds


Brain - Lead, mercury and compounds

Lungs - Ammonia, NOx, SOx ,asbestos, coal

and other dusts

Kidneys - Mercury, cadmium and compounds

Liver - Chlorinated hydrocarbons,

ethylene, chlorohydrin and alcohol


Nerves - Solvents, organophosphates, mercury,

manganese and lead

Bladder - auramine, benzidine, 

napthylamine

Bone marrow - Benzene

Skin - Detergents, solvents, mineral oils, acids, alkalis

and chlorinated hydrocarbons, arsenic, chromium, and

nickel
ROUTE OF ENTRY

For a chemical to have an effect on a worker, they


must be exposed to it and some of it must get into
their system
 Inhalation/breathing – most common route, gases/vapors
can pass to blood, solid particles inhaled into lungs.
 Absorption through the skin – many solids, liquids, vapors
and gases can be absorbed through the skin.
 Ingestion / swallowing – while not intentional, failure to
wash hands, eating in contaminated lab, etc.
 Injection – accidents handling glass, sharps, etc.
 Eye contact – either physical damage or absorption

The route of entry dictates protective equipment


BIOLOGICAL/CHEMICAL EXPOSURES

Needle Sticks: Ocular Exposures: Dermal Exposures:

Wash thoroughly with soap and water Rinse at eyewash at least 15 minutes Rinse under drench shower at least 15
minutes

Identify source (consent?) Hold eye open to ensure effective Remove all contaminated clothing
wash

 Report to Campus clinic

 You must fill out an Incident Report


EMERGENCY PROCEDURES

 If an irritating or harmful reagent


splashes onto your body, use the
emergency head shower to wash your
body for several minutes (at least 15
minutes).
 If a reagent spills onto the workbench or
floor, notify the Laboratory supervisor
immediately for cleanup instructions.
 If a glass container gets broken, notify
the Lab Supervisor immediately for
cleanup instructions.
MECHANICAL HAZARDS

ENtanglement

Traps

Impact

Contact

Ejection
ENTANGLEMENT

Entanglement: Clothing, Hair, Jewellery getting wrapped around machinery


TRAPS

Traps involve:

Shearing

Drawing in

In running nips

Crushing
IMPACT
ELECTRICAL SAFETY DO’S AND DON’TS

 Carryout regular visual checks for damage – disconnect first.


 Don’t use faulty or damaged equipment.
 Use proper plugs & connections (fused /earthed).
 Avoid the use of multi- boxes, extension cords and trailing cables.
 Turn off non-essential electrical equipment at the end of the day.
 Don’t insert fingers / metal objects to clear jams in photocopiers
/shredders.
 If a plug sockets gets hot there is something wrong. Don’t use it-report it.
 Always ensure that all equipment and electrical cables are inspected.
 Keep liquid/water away from all electrical equipment.
 Treat all electrical equipment as live.
 Never attempt electrical repairs
 Never overload sockets
PARTICIPANT ACKNOWLEDGEMENT FORM

This acknowledgement form is to be completed

by the participant and returned back to the lab

supervisor before leave the lab.

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