Scribd
Upload
330 views9 pages

Frog Muscle Twitch Experiment Setup

1. The document describes how to set up a frog muscle preparation to observe muscle twitches and perform muscle work experiments. 2. Key steps include removing the gastrocnemius muscle from a frog, attaching one end to a clamp and the other to a transducer lever, and positioning stimulator electrodes along the muscle to deliver electrical stimuli. 3. The experiment aims to determine the threshold stimulus needed to induce a contraction by gradually increasing stimulus strength until the smallest amount that causes a twitch is found.

Uploaded by

Hans Kimberly V. David
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
330 views9 pages

Frog Muscle Twitch Experiment Setup

1. The document describes how to set up a frog muscle preparation to observe muscle twitches and perform muscle work experiments. 2. Key steps include removing the gastrocnemius muscle from a frog, attaching one end to a clamp and the other to a transducer lever, and positioning stimulator electrodes along the muscle to deliver electrical stimuli. 3. The experiment aims to determine the threshold stimulus needed to induce a contraction by gradually increasing stimulus strength until the smallest amount that causes a twitch is found.

Uploaded by

Hans Kimberly V. David
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

KYMO II

ASSEMBLY FOR MUSCLE


TWITCH
AND MUSCLE WORK
EXPERIMENTS
Group 2
Set up the frog muscle preparation as follows:

1. We can observe a muscle twitch by removing the gastrocnemius


from a frog and administering a small stimulus to a muscle. Compress
the femur in the femur clamp. The muscle was left attached to part of
the femur,

2. Adjust the clamps so that the thread is vertical and the


displacement transducer rod rests just on the upper stop with no slack
in the thread. For the Gastrocnemius muscle, one end of a thin, straight
wire was tied to the tendon and the other end attached to an isometric
lever.
3. Position the stimulator electrodes so that they lie against the muscle about
midway between the knee and the tendon; the two electrodes should not
touch one another, but both must be in contact with the muscle. In here,
we will determine the threshold stimulus. A threshold stimulation is the
smallest amount of stimulation that will actually result in a contraction. A
minimum amount of stimulation is needed to induce a muscular
contraction. We can stimulate the muscle with an external power source. By
gradually increasing the amount of stimulus strength, we can find the
threshold level of stimulation.
4. The Double Right Angle Clamp will ensure to support the gastrocnemius
muscle in the whole experiment. Adjust the thread tension by moving the
transducer up or down, until the lever is horizontal. Make sure that the
thread is aligned at a 90 degree angle to the transducer lever. It holds and
provides support to the rod that contains pen allowing the pen to write on
the graphing paper in your kymograph.  In the vertical bar those clamp are
use to hold tranduscer rods, and another rod for holding the frog muscle
usually gastronecmius muscle

You might also like