Scribd
Upload
51 views7 pages

Analisis Protein: Metode Kjeldahl & Formol

1. This document describes two methods for analyzing protein content: the Kjeldahl method and the formol titration method. 2. The Kjeldahl method involves digesting a sample in acid and catalyst, distilling the ammonia produced, and titrating to determine nitrogen content. Nitrogen content is then converted to a protein percentage using conversion factors. 3. The formol titration method involves reacting a sample with formaldehyde and potassium oxalate, then titrating with sodium hydroxide. The titration volume corresponds to protein content using specified conversion factors.

Uploaded by

putra_106
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
51 views7 pages

Analisis Protein: Metode Kjeldahl & Formol

1. This document describes two methods for analyzing protein content: the Kjeldahl method and the formol titration method. 2. The Kjeldahl method involves digesting a sample in acid and catalyst, distilling the ammonia produced, and titrating to determine nitrogen content. Nitrogen content is then converted to a protein percentage using conversion factors. 3. The formol titration method involves reacting a sample with formaldehyde and potassium oxalate, then titrating with sodium hydroxide. The titration volume corresponds to protein content using specified conversion factors.

Uploaded by

putra_106
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

ANALISIS Protein

1. P.K. N total cara Gunning


- Timbang 0,7 – 3,5 g bhn (dihaluskan), mskk
dlm labu Kjeldahl, + 10 g K2S atau Na2SO4
anh, + 15 – 25 ml H2SO4 pkt (Kl perlu di+ 0,1 –
0,3 g CuSO4) dikocok.
- Panaskan pd pemanas listrik atau api bunsen
dlm lemari asam dg api kecil. Setlh asap
hilang api dibesarkan, panaskan sp lrtan
jernih.
- Buat percobaan blanko (tanpa sampel).
- Setlh labu + cairan dingin, + 200 ml aq dem +
1 g serbuk Zn + lrtan NaOH 45% sp cairan
bersifat basa. Sgr pasang labu pada alat
destilasi.
- Panaskan labu sampai amonia menguap
semua, destilat ditampung dlm Erlenmeyer
yg berisi 100 ml HCl 0,1 N + indik fenol merah
atau metil merah.
- Destilasi diakhiri setlh volume destilat 150 ml atau sp destilat
tak bersft basa.
- Keleb HCl 0,1 N dlm destilat dititrasi dg lrtan baku NaOH 0,1N.

Perhitungan:
% N = (ml NaOH blanko – ml NaOH sampel) X NNaOH x 14,008
g sampel x 10

% protein = % N x faktor (lht tabel)


Konversi dari kadar N menjadi kdr protein berbagai macam bhn

No Bahan Faktor konvensi

1 Bir,sirup biji2-an, ragi, mknan ternak, buah2an, teh, 6,25


malt,anggur
2 Beras 5,95

3 Roti,gandum, makaroni, bakmi 5,70

4 Kacang tanah 5,46

5 Kedelai 5,75

6 Kenari 5,18

7 Susu kental manis 6,38


2. P.K. Protein dlm susu cara Formol
- Pipet 10,0 ml susu atau cairan protein ke dlm Erlenmeyer 125
ml + 20 ml aq dem + 0,4 ml lrtan K-ox jenuh (K-ox : air = 1:3) +
1 ml p.p. 1%. Diamkan 2 menit.
- Titrasi lrtan dg NaOH 0,1 N sp wrn spt wrn standard di bwh ini
atau sp merah jambu.
- Warna standard: 10,0 ml susu + 10 ml aq dem + 0,4 ml K-ox
jenuh + 1 tts 0,01% indik rosanilin-chlorida (lamp 17).
- Setlh wrn tercapai + 2 ml lrtan formaldehid 40%, titrasi
kembali dg lrtan NaOH p wrn spt wrn standard lagi.
- Catat volume titrasi kedua.
- Titrasi blanko: 20 ml aq dem + 0,4 ml lrtan K-ox jenuh + 1 ml
ind p.p. + 2 ml lrtan formaldehid, titrasi dg NaOH.
- Titrasi terkoreksi: titrasi kedua dikurangi titrasi blanko = titrasi
formol.
- Utk mengetahui % protein dibuat percobaan serupa dg
menggnk lrtan yg tlh diket kdr prot nya (mis dg cara Kjeldahl)
- Untuk susu dapat digunakan faktor 1,83:

% prot susu = 1,83 x ml titrasi formol

% kasein = 1,63 x ml titrasi formol

% N = titrasi formol x NNaOH x 14,008/g bahan x 10


Reaksi titrasi formol:

H O O
H
R C HC NaOH R C HC
OH + O-
NH2 NH3
pada pH netral

H O H
R C HC R C COOH
+ CH2O
O- formalin
NH3+ HOH2C N CH2OH
dimethilol

H H
R C COOH R C COONa
+ NaOH
HOH2C N CH2OH HOH2C N CH2OH
dimethilol

You might also like