Biogenesis vs.
Abiogenesis
Biology 11
The secret to reaching my goals lies solely in my tenacity
-Louis Pasteur
Abiogenesis vs. Biogenesis
Introduction: Biology as a Science progressed by observation. The hypotheses proposed by early scientists were rarely tested by experiments.
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Example: Pond dries up no fish or frogs Rain comes fish and frogs Therefore: It rained fish and frogs
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Theory of Abiogenesis (aka spontaneous generation) Nonliving things can be transformed into living things. Proposed by Aristotle who observed rotting meat and flies. Existed for nearly 2000 years
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~300 years ago Belgian doctor Jean van Helmont concluded that mice could be created from a dirty shirt. He placed grains of wheat and a dirty shirt in a container and in 21 days mice appeared. Conclusion: Sweat caused wheat to ferment into mice. Abiogenesis!
Redi
In 1668 Francesco Redi (Italian physician) tested Aristotles hypothesis (meat flies)
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4 sets of jars with meat (snake, eel, fish, veal) one set was open (experimental group) one set was sealed (control group) Maggots appeared on open jars of meat Conclusion: Flies come from flies! Biogenesis life from life.
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Bad news lots of critics No air in jar, therefore life could not survive. Redi repeated his experiment with a fine mesh wire supported Biogenesis again! Critics still objected
Needham
John Needham (1713 1781) English botanist Reexamined theory of Abiogenesis Observed meat broth left unsealed soon changed colour (evidence of microorganisms).
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Experiment: Boiled flasks of meat broth to kill microorganisms Left the flasks (unsealed) Flasks soon become murky and full of microbes
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Conclusion: Abiogenesis AHHHHHHHHH!!!
Sent many Scientists down the wrong path.
Lazzaro Spallanzani (1729-1799)
25 years later Spallanzani repeated experiment more carefully Boiled flasks longer and sealed flasks Critics again objected to sealed flasks!
The Final Blow!
Louis Pasteur (1822-1895)
1864 Pasteur developed a swan-neck flask
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Experiment: Boiled broth, air could enter but microbes were trapped in curved flask Broth remained clear Biogenesis finally proven!
The End!
Now here is your assignment: List and explain three flaws in experimental design that you have heard about during this presentation.