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4.8.05 AOAC of Fi Cial Method 943.01 Chlo Rine (Sol U Ble) in An I Mal Feed

The document outlines the AOAC Official Method 943.01 for determining soluble chlorine in animal feed using a titrimetric method. It details the preparation of reagents, the procedure for sample preparation, and the titration process to measure chlorine content. Specific aliquot volumes are recommended based on the chlorine concentration in the feed materials.

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36 views1 page

4.8.05 AOAC of Fi Cial Method 943.01 Chlo Rine (Sol U Ble) in An I Mal Feed

The document outlines the AOAC Official Method 943.01 for determining soluble chlorine in animal feed using a titrimetric method. It details the preparation of reagents, the procedure for sample preparation, and the titration process to measure chlorine content. Specific aliquot volumes are recommended based on the chlorine concentration in the feed materials.

Uploaded by

DaNnaGArcia
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd

4.8.05 swirling is necessary.

If solution is agitated by vigorous vertical


AOAC Official Method 943.01 shaking, filtration will be difficult.) Let mixture settle 10 min. Filter
Chlorine (Soluble) in Animal Feed through dry 11 cm Whatman No. 41 paper, or equivalent. Use 50 mL
Titrimetric Method aliquots ( 13 of total) on feed materials low in Cl (0–2% Cl) and 25 mL
First Action 1943 aliquots ( 16 of total) on feed materials high in Cl (>2%). For mineral
Final Action 1945 and other feeds containing 10% Cl, weigh 1 g and use 15 mL ( 110 of
A. Reagents total).
If approximate percent Cl in feed materials is not known, take
(a) Potassium chloride standard solution.—0.001 g Cl/mL.
10 mL aliquot for trial titration. To this add 10 mL HNO3 and 10 mL
Recrystallize reagent KCl 3 times from H2O, dry at 110°C, and heat at
Fe2(SO4)3 indicator. Dilute to ca 50 mL. Add 0.5 mL KSCN
ca 500°C to constant weight. Dissolve 2.1028 g in H2O and dilute to
solution and immediately add, with stirring, enough AgNO3
1 L.
solution to entirely eliminate any reddish color. From this titration
(b) Silver nitrate solution.—Dissolve 5 g AgNO3 in 1 L H2O and calculate volume AgNO3 solution necessary to precipitate all Cl in
adjust solution so that 1 mL = 1 mL standard KCl solution. aliquot to be used, adding excess equal to ca 10% total volume
(c) Potassium thiocyanate solution.—Dissolve 2.5 g KSCN in necessary, although somewhat greater excess will not affect
1 L H2O and adjust so that 1 mL = 1 mL standard AgNO3 solution. results. Use minimum total of 10 mL.
Standardize as in 942.26C (see A.1.02). To test solution aliquot in 250 mL beaker add 10 mL HNO3 and
(d) Ferric sulfate solution.—Dissolve 60 g Fe2(SO4)3×12H2O in 10 mL Fe2(SO4)3 indicator (or 20 mL solution containing equal
H2O and dilute to 1 L. volumes of these solutions). Add, with stirring, calculated volume
(e) Ferric sulfate indicator.—To filtered 25% solution of AgNO3 solution. Heat to boiling and cool to room temperature,
Fe2(SO4)3×12H2O, add equal volume HNO3. stirring enough to coagulate precipitate. (Cooling may be hastened
by immersion of beakers in cold water.) Titrate excess AgNO3 with
B. Determination
KSCN. End point is indicated by first appearance of reddish tint that
Transfer 3 g test portion to 300 mL Erlenmeyer. Add 50 mL persists 15 s. For accurate work, use reference solution containing
Fe2(SO4)3 solution (accurately measured), swirling flask to prevent all ingredients except KSCN. End point is first change in color.
caking of test portion and to facilitate solution of Cl. Add 100 mL
(also accurately measured) NH4OH (1 + 19). Swirl flask just enough References: JAOAC 26, 87(1943); 28, 80(1945).
to ensure solution of Cl and thorough mixing of solution. (Very little CAS-7782-50-5 (chlorine)

ã 2008 AOAC INTERNATIONAL

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