Sri Padmavati School of Pharmacy (JNTUA)
Mohan Gardens, behind R.K. Kalyanamandapam, Vaishnavi Nagar, , , Tirupati
Andhra Pradesh 517503 India
User Details
User Name: Prof. Dr D Ranganayakulu - Institute Group Admin
Email: spspprinci@[Link]
Date & Time: Nov,29,2025 [Link] PM
EXPERIMENT Biossay of Serotonin on Rat Stomach Strip by Three-Point Assay Method
(Tutorial Mode)
OBSERVATION TABLE
SI. Conc. of Amount Added to Amount added to Conc. in organ Response
No. Serotonin (µg/mL) Organ Bath (mL) organ tube (µg) tube (µg/mL) (mm)
1 20 0.002 0.04 0.004 2
2 20 0.005 0.1 0.01 4
3 20 0.01 0.2 0.02 6
4 20 0.02 0.4 0.04 8
5 20 0.05 1 0.1 10
6 20 0.1 2 0.2 12
7 20 0.2 4 0.4 14
8 20 0.4 8 0.8 16
9 20 0.8 16 1.6 18
Test Solution of Serotonin
SI. No. Amount Added to Organ Bath (mL) Response (mm)
1 0.002 1
2 0.005 3
3 0.01 5
4 0.02 7
5 0.05 9
6 0.1 11
7 0.2 13
8 0.4 15
9 0.8 17
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n1 n2 t
0.02 0.05 0.01
Response of n1 = 12 Response of n2 = 5 Response of t = 14
Response of n2 = 5 Response of t = 14 Response of n1 = 12
Response of t = 14 Response of n1 = 12 Response of n2 = 5
SI. No Amount added to organ bath (mL) Average of Response (mm)
1 n1 0.02 S1 12
2 n2 0.05 S2 5
3 t 0.01 T 14
S1 = Average of responses of n1
S2 = Average of responses of n2
T = Average of responses of t
Concentration of Serotonin Test Solution
= (n1/t) x antilog {(T-S1) / (S2-S1) x log(n2/n1)} x standard conc.
= (0.02 / 0.01 ) * antilog {( 14 - 12 ) / ( 5 - 12)} * log (( 0.05 / 0.02 )) } * 20
The concentration of Serotonin Test solution is = 30.788 (µg/mL)
The actual concentration of given Serotonin Test Solution : 30 µg/mL
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Logs
Date & Time Log
29-11-2025 [Link] 0.002 ml of Serotonin ( 20 microgram/ml ) was added
29-11-2025 [Link] 0.005 ml of Serotonin ( 20 microgram/ml ) was added
29-11-2025 [Link] 0.01 ml of Serotonin ( 20 microgram/ml ) was added
29-11-2025 [Link] 0.02 ml of Serotonin ( 20 microgram/ml ) was added
29-11-2025 [Link] 0.05 ml of Serotonin ( 20 microgram/ml ) was added
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Date & Time Log
29-11-2025 [Link] 0.1 ml of Serotonin ( 20 microgram/ml ) was added
29-11-2025 [Link] 0.2 ml of Serotonin ( 20 microgram/ml ) was added
29-11-2025 [Link] 0.4 ml of Serotonin ( 20 microgram/ml ) was added
29-11-2025 [Link] 0.8 ml of Serotonin ( 20 microgram/ml ) was added
29-11-2025 [Link] 0.002 ml of Serotonin ( Test microgram/ml ) was added
29-11-2025 [Link] 0.005 ml of Serotonin ( Test microgram/ml ) was added
29-11-2025 [Link] 0.01 ml of Serotonin ( Test microgram/ml ) was added
29-11-2025 [Link] 0.02 ml of Serotonin ( Test microgram/ml ) was added
29-11-2025 [Link] 0.05 ml of Serotonin ( Test microgram/ml ) was added
29-11-2025 [Link] 0.1 ml of Serotonin ( Test microgram/ml ) was added
29-11-2025 [Link] 0.2 ml of Serotonin ( Test microgram/ml ) was added
29-11-2025 [Link] 0.4 ml of Serotonin ( Test microgram/ml ) was added
29-11-2025 [Link] 0.8 ml of Serotonin ( Test microgram/ml ) was added
29-11-2025 [Link] 0.02 ml of Serotonin ( n1 microgram/ml ) was added
29-11-2025 [Link] 0.05 ml of Serotonin ( n2 microgram/ml ) was added
29-11-2025 [Link] 0.01 ml of Serotonin ( t microgram/ml ) was added
29-11-2025 [Link] 0.05 ml of Serotonin ( n2 microgram/ml ) was added
29-11-2025 [Link] 0.01 ml of Serotonin ( t microgram/ml ) was added
29-11-2025 [Link] 0.02 ml of Serotonin ( n1 microgram/ml ) was added
29-11-2025 [Link] 0.01 ml of Serotonin ( t microgram/ml ) was added
29-11-2025 [Link] 0.02 ml of Serotonin ( n1 microgram/ml ) was added
29-11-2025 [Link] 0.05 ml of Serotonin ( n2 microgram/ml ) was added
29-11-2025 [Link] Report generated
29-11-2025 [Link] Report generated
29-11-2025 [Link] Report generated
MCQs
Q.1 Selective serotonin reuptake inhibitors are used in treatment of ____.
User Ans: c. chemotherapy induced emesis
Correct Ans: (c) chemotherapy induced emesis
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Q.2 The rapeutic indication of serotonin agonist Sumatriptan is _____.
User Ans: c. migraine
Correct Ans: (c) migraine
Q.3 Rat fundus strip is a _____.
User Ans: b. smooth muscle
Correct Ans: (b) smooth muscle
Q.4 Chemotherapy induced emesis involves role of _____.
User Ans: d. serotonin
Correct Ans: (d) serotonin
Q.5 About 90% of the body content of serotonin is restricted to ________.
User Ans: a. intestine
Correct Ans: (a) intestine
Exercise
Exercise 1: What are advantages and disadvantages of different types of
bioassays?
User Ans: Advantages: this graphical method
No calculation is required
Disadvantages: the quantity of test required is larger
Perliminary response is not used in the determination of potency
Correct Ans: Interpolation Type Bioassays. The advantages are: Entire dose
range is considered while determining the concentration. This is a graphical
method. No calculations are required. Results are comparatively accurate. The
errors in reading are distributed all through. From the slope of the curve
reliability and precision of the method can be determined. The method is
comparatively less time consuming. The disadvantage is: The quantity of the
test sample required is larger. Matching Type Bioassay, The advantage is: The
quantity of test sample required is comparatively smaller. The disadvantages
are: Preliminary responses are not used in determination of potency.
Experimental error cannot be determined. It does not take into consideration
parallelism of DRCs, nor the efficacy of the drugs. Only particular dose levels
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are compared hence results are not accurate. Useful when the concentration of
the test material is very less. Bracketing Assay, The advantage is: This method
does not take into consideration the parallelism of the DRCs of standard and
test drugs. Only the particular doses are compared. The disadvantages are:
Initial higher dose can reduce the sensitivity of the tissue. The preliminary doses
are not considered while determining the potency. Experimental error can not be
determined. Multiple Point Assay, The advantages is: Less amount of the test
drug is used and results are more accurate. Readings are taken at dose levels
to which the tissue is more sensitive.
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