DETAILED LESSON PLAN IN GRADE 8
Division: Antique Grade Level: Grade 8
Name of Teacher: Benny S. Bendita Jr. Learning Area: Biotechnology
Teaching Date & Time: Week 3 (Day 1-2) Quarter: 2nd Quarter
Duration: 60 minutes
Learning Area: Biotechnology 8 Quarter: 2 Week: 3
I.
OBJECTIVES
A. Content Demonstrates an understanding of the basic
Standard tools used in genetic engineering to
manipulate DNA for various purposes,
including biotechnology applications.
B. Performance Describe and differentiate between the
Standard various tools used in genetic engineering,
explaining their functions and applications
in manipulating genetic material.
C. Learning Describe the Different Tools Used in
Competency with Genetic Engineering (S8LT-IIIa-b-16)
Code
D. Learning At the end of the lesson, the Grade 8
Objectives learners should be able to perform the
following tasks with 80% proficiency:
1. Describe the tools used in genetic
engineering and their functions
2. Demonstrate the action of a restriction
enzyme in DNA manipulation
3. Reflect on the ethical implications of
genetic engineering
II. CONTENT
Topic: Tools Used in Genetic Engineering
Subject Ethics, Technology
Integration:
Value Focus: Cooperation/ Participation
Critical Thinking
Responsibility
III. LEARNING
RESOURCES
A. References
A.1. Teacher’s Essential Learning Competencies of DepEd
Guide Pages for Special Science Program
A.2. Learner’s None
Material Pages
A.3. Textbook
Pages
B. Other Learning PowerPoint Presentation, Video
Resources Presentation, Genetic Engineering Tool
Cards or Flashcards, Handouts with
descriptions on genetic engineering tools
IV. TEACHER’S ACTIVITIES/ STUDENT’S ACTIVITIES/
PROCEDURES RESPONSES RESPONSES
Preliminaries (Expected Answers)
Greetings Good morning class Good morning, Sir Bendita.
Prayer May I request anyone from the
group to please lead the prayer? (one student will volunteer)
Before taking your seats, may I
Classroom request everyone to please pick up
managemen the rubbish under your chair and
t after that, please arrange your chair
and sit quietly
Before we start, may I request the
class monitor to please check the (The classroom monitor will
Checking of attendance and submit to me later check the attendance)
Attendance on the name of absentees for today
Lesson Proper
a. Elicit Last time, we discussed the techniques or
practices used in Traditional and Modern
Biotechnology (cheese, toyo, vinegar
making, nata de coco production and
etc…).
Last time we discussed about the
Any idea about it? different techniques or practices
used in Traditional and Modern
Yes,(name of the student) Biotechnology (cheese, toyo,
vinegar making, nata de coco
production and etc…).
Very good class! But before that, I have a
question, “What do you know about DNA
and its role in genetics?”
Yes, (name of the student) DNA is the molecule that carries
genetic instructions, determining
traits, enabling heredity, and
guiding protein synthesis and
genetic variation in living
organisms.
Very good.
Have you heard of the word genetic All: Yes Sir.
engineering?
Okay, in order to answer my question, let’s
have first an activity. Please group
yourselves into 2 groups. What you’re (The class will create a two
going to do is to create a concept map out groups and the two group leaders
of the word genetic engineering and the will pick a topic in the front)
role of DNA in genetics. In this activity, the
two group leaders will pick here in front
whether which of the two topics you’re
going to answer.
b. Engage So, please settle down now and in order for
us to check whether your answers are
correct, let’s have first a video presentation
about genetic engineering in action, such as
CRISPR editing gene in a laboratory
setting.
(The video presentation will play)
(https://youtu.be/CDw4WPng2iE?
si=jHG7-PV9tPZ4qj2g)
Guide Questions:
1. What tools do you think scientists use to
modify the DNA of organisms?
The tools that scientists use to
Yes, (name of the student) modify the DNA of organisms are
CRISPR-Cas9, Restriction
Enzymes, DNA Ligase,
Polymerase Chain Reaction
(PCR), Plasmids, Gene Guns,
Electroporation, Zinc-Finger
Nucleases (ZFNs), and Viral
Vectors
Fantastic.
2. What are the real-world applications of
genetic engineering?
Medicine (Gene Therapy,
Yes, (name of the student) Production of Insulin,
Development of Vaccines, Cancer
Treatments, Pharmaceuticals
Production)
Agriculture (Genetically Modified
Crops, Herbicide-resistant Plants,
Disease-resistant Plants)
Industry (Bio-manufacturing,
Textile Industry, Food Industry)
Environmental Applications
(Bioremediation, Carbon
Sequestration)
Animal Applications (Transgenic
Animals, Xenotransplantation)
Forensics and Research (DNA
Fingerprinting, Model Organisms)
Biosecurity (Pathogen Resistance,
Biocontainment)
Impressive.
I think some of you has a knowledge of
what will be our topic for today. May I
request anyone from the group to read our
learning objectives for us to be guided as One student will read the learning
we go along with our topic for today? objectives
Yes, (name of the student)
Okay, thank you (name of the student)
c. Explore Our topic for today is all about tools used
in genetic engineering. Before we proceed
to our topic for today, let me introduce
some of the descriptions of the genetic
engineering tools that we discussed earlier.
Restrictions Enzyme: enzymes that cut
DNA at specific sequences
Polymerase Chain Reaction (PCR):
technique used to amplify small DNA
samples
Cloning Vectors: DNA molecules used to
carry foreign genetic material into cells
CRSIPR-Cas9: a tool for precise gene
editing
Gel Electrophoresis: a method for
separating DNA fragments based on size
( The class will divide themselves
into two groups)
Group yourselves into two groups. I have
here different pictures printed on a paper.
What you’re going to do is that identify
what genetic engineering tools are showed
based on the descriptions that I was given
earlier.
d. Explain PowerPoint presentation is also presented
as the guide for a smooth flow of the topic
including the introduction of the tools like
restriction enzymes, ligases, and CRISPR
and their roles.
1. CRISPR-Cas9
Significance:
A revolutionary gene-editing tool
that allows precise targeting and
modification of DNA sequences
Highly efficient, cost- effective, and
versatile compared to earlier
method
Applications:
Gene Therapy: Treats genetic
disorders like sickle cell anemia by
repairing faulty genes
Medical Advancements: Used to
study and develop treatments for
diseases such as cancer and HIV.
GMOs: Creates pest-resistant crops
and improves livestock genetics for
better yields.
2. Restriction Enzyme
Significance:
Known as molecular scissors, these
enzymes cut DNA at specific
sequences, enabling the isolation of
genes or DNA fragments for
modification.
Applications:
Recombinant DNA Technology:
Creating genetically engineered
bacteria to produce insulin and
other pharmaceuticals.
GMOs: Facilitates the insertion of
beneficial traits into plants, such as
drought resistance.
3. DNA Ligase
Significance:
Joins DNA fragments together,
essential for assembling
recombinant DNA molecules.
Applications:
Medicine: Helps construct synthetic
genes for therapeutic purposes.
GMOs: Enables the insertion of
new genes into plant genomes for
better crop traits.
4. Polymerase Chain Reaction (PCR)
Significance:
Amplifies specific DNA sequences,
making them easier to analyze or
manipulate.
Applications:
Diagnostics: Detects genetic
mutations linked to diseases like
cancer.
Gene Therapy: Amplifies target
genes for editing or replacement.
Agriculture: Identifies genetic
markers in crops for breeding
programs.
5. Plasmids
Significance:
Circular DNA molecules that act as
vectors to transfer genetic material
into host cells.
Applications:
GMOs: Produces genetically
modified bacteria for industrial
enzyme production.
Gene Therapy: Delivers therapeutic
genes to cells for treating diseases
like hemophilia.
6. Gene Gun
Significance:
Delivers DNA- coated microscopic
particles into cells, enabling direct
gene transfer.
Applications:
GMOs: Introduces beneficial genes
into crops, such as vitamin-
enriched Golden Rice.
Biotechnology: Facilitates genetic
engineering in plants and animals.
7. Electroporation
Significance
Uses electrical pulses to
temporarily create pores in cell
membranes, allowing DNA entry.
Applications:
Gene Therapy: Transfers
therapeutic DNA into patient cells.
GMOs: Modifies microorganisms
for industrial applications like
biofuel production.
8. Viral Vectors
Significance:
Modified viruses act as carriers to
deliver genetic material into cells
Applications:
Gene Therapy: Treats inherited
disorders like cystic fibrosis by
delivering healthy genes.
Medical Advancements: Develops
viral-based vaccines, such as
mRNA COVID-19 vaccines.
9. Zinc-Finger Nucleases (ZFNs)
Significance:
Engineered proteins that create
precise breaks in DNA for gene
editing.
Applications:
Gene Therapy: Treats conditions
like HIV by editing immune cell
genes.
Agriculture: Improves plant traits,
such as resistance to fungal
diseases.
10. TALENs ( Transcription Activator-
Like Effector Nucleases)
Significance:
Customizable proteins for editing
specific DNA sequences with high
precision.
Applications:
Gene Therapy: Corrects genetic
defects in diseases like muscular
dystrophy.
GMOs: Develops improved crops
with traits like higher nutritional
value.
Summary:
Gene Therapy
Tools like CRSIPR-Cas9, ZFNs, and
viral vectors repair or replace defective
genes to treat diseases like cancer, sickle-
cell anemia, and muscular dystrophy.
Genetically Modified Organisms (GMOs)
Techniques using plasmids, gene guns,
and CRISPR create crops with enhanced
yields, pest resistance, and improved
nutritional value.
Medical Advancements
PCR and CRISPR accelerates the
development of treatments and diagnostics
for genetic disorders, while recombinant
DNA technology produces life-saving
drugs like insulin.
Are we clear?
Yes, Sir.
e. Elaborate Okay very good! This class is very amazing
for everyone is showing enthusiasm in
listening our topic for today.
With your same groupmates, each group (the students will go to their
will create a graphic organizer about the respective groupmates)
ethical issues surrounding genetic
engineering. What are the possible benefits
and risks of it, and later on, each group
representative will explain the work of their
group in front.
Rubrics
Balance: Highlight both the potential
benefits and risks of genetic engineering to
show critical thinking (30%)
Depth: Use specific examples, such as
CRISPR in treating diseases or GMOs in
agriculture, to add credibility (20%)
Ethics: Address ethical concerns like
equity, safety, and environmental
sustainability to provide a well-rounded
view (20%)
Creativity: Make the graphic appealing to
help communicate complex ideas
effectively (30%)
Total: 100%
f. Evaluate To check your understanding for today’s
lesson, let’s have a short quiz first in order
for me to evaluate whether you listen to my
discussion today.
Part I. Multiple Choice
1. This tool acts like molecular scissors,
cutting DNA at specific sequences. C
A. DNA Ligase
B. Restriction Enzymes
C. CRISPR-Cas9
D. Polymerase Chain Reaction (PCR)
2. This technique amplifies DNA sequences
to make them easier to analyze or modify.
A. PCR A
B. Gene Gun
C. Electroporation
D. Viral Vectors
3. A protein-based tool that can edit DNA
with high precision by cutting and
replacing specific sequences. D
A. DNA Ligase
B. Plasmids
C. CRISPR-Cas9
D. TALENs
4. Circular DNA molecules often used as
vectors to transfer genetic material into a
host organism.
A. DNA Ligase B
B. Plasmids
C. Gene Gun
D. Restriction Enzymes
5. This method uses electrical pulses to
make cell membranes permeable to DNA.
A. Electroporation
B. Gene Gun A
C. PCR
D. TALENs
6. These modified viruses deliver genes
into cells, commonly used in gene therapy.
A. Viral Vectors
B. CRISPR-Cas9 A
C. Plasmids
D. ZFNs
7. This tool joins DNA fragments, such as
inserting a new gene into a plasmid.
A. DNA Ligase
B. Restriction Enzymes A
C. PCR
D. Gene Guns
8. This tool shoots DNA-coated particles
into cells to introduce new genetic material.
A. Gene Gun A
B. Electroporation
C. PCR
D. Viral Vectors
9. Engineered proteins used to create
breaks in DNA for editing, similar to
CRISPR.
A. Plasmids B
B. TALENs
C. DNA Ligase
D. PCR
10. This method uses proteins that bind to
specific DNA sequences to edit genes,
often used before CRISPR. C
A. CRISPR-Cas9
B. Gene Gun
C. ZFNs
D. Electroporation
Part II. Matching Types
Match the column A with the
corresponding answer in column B.
Column A Column B
11. PCR a. Treating genetic B
disorders
like cystic fibrosis
12. CRISPR- b. Amplifying DNA A
Cas9 sequences
13. Viral Vectors c. Joining DNA D
fragments C
14. DNA Ligase d. Delivering genetic
material into cells E
15. Gene Gun e. Introducing DNA
into
plants for pest
resistance
Part III. Essay (5pts)
Write one paragraph about the ethical
implications of genetic engineering,
demonstrating their understanding of the
topic.
g. Extend Research the latest advancements in genetic
engineering technologies (such as
CRISPR’s potential in medicine) and
prepare a brief report on its potential future
applications and ethical considerations.
References: https://www.nature.com/scitable
https://www.genome.gov
https://www.crisprtx.com
National Human Genome Research Institute (NHGRI)
Klug, W. S., Cummings, M. R., Spencer, C. A. & Palladino, M. A. (2016). Concepts of genetics (12th
ed.). Pearson
PREPARED BY: CHECKED AND VERIFIED BY:
BENNY S. BENDITA JR. JOINVILLE M. OMAY
Student Intern Cooperating Teacher
