REPLICATIONS
(TIFR 2010)
If a species contains 23% adenine in its DNA, then the percentage of guanine in its
DNA would be:
(1) 23%
(2) 46%
(3) 54%
(4) 27%
REPLICATIONS
(TIFR 2014)
The genome of a virus has a composition of 25% G, 25% C. 30% A and 20%U. The
genome is a
(1) ssDNA
(2) dsDNA
(3) ssRNA
(4) dsRNA
REPLICATIONS
(TIFR 2014)
In the Meselson-Stahl DNA replication experiment, what percent of the DNA was
composed of one light strand and one heavy strand after one generation of growth in
"N containing growth media?
(1) 25
(2) 50
(3) 75
(4) 100
REPLICATIONS
(TIFR 2015)
If the ratio of (A+G)/(T+C) in one strand of DNA is 0.70, what is the same ratio in
the complementary strand?
(1) 0.70
(2) 1.42
(3) 0.35
(4) 0.30
REPLICATIONS
(TIFR 2017)
During growth and division of E. coli, the daughter strand is recognized due to
(1) Hemi-methylation of newly synthesized DNA
(2) Nicks in newly synthesized DNA
(3) Double stranded breaks in newly synthesized DNA
(4) DNA damage in newly synthesized DNA
REPLICATIONS
(TIFR 2017)
What is the correct order of action of these enzymes. during DNA replication?
(1) Helicase, primase, polymerase
(2) Helicase, polymerase, primase
(3) Primase, helicase, polymerase
(4) Polymerase, primase, helicase
REPLICATIONS
(TIFR 2022)
An altered form of a replicative DNA polymerase lacks 3‘---͢5‘ exonuclease activity.
This alteration would most likely result in which of the following
(1) A decrease in processivity
(2) An increased mutation rate
(3) An inability to replicate DNA
(4) An inability to remove RNA primers
REPLICATIONS
(TIFR 2022)
A mammalian cell typically has 1.2 meters (where completely outstretched) of
double stranded DNA The total time to duplicate the DNA is 5 hours. How many
origins of replication are there if the rate of duplication is 16µmeters/min?
(1) 2500
(2) 25
(3) 250
(4) 500
TRANSCRIPTION & RNA SPLICING
(TIFR 2016)
A mutation in the 3 untranslated region of an mRNA has a strong phenotype. The
phenotype could be because:
(1) The mRNA fails to be transcribed
(2) The mRNA is transcribed but is unstable
(3) The mRNA is not properly translated to protein
(4) The protein translated from the mRNA is unstable
TRANSCRIPTION & RNA SPLICING
(TIFR 2018)
You transcribe mRNA from a DNA sample and purify it. You then separate the two
strands of the DNA and analyse the base composition of each strand and of the
mRNA. You obtain the data shown in the table below. Which strand of the DNA is
serving as a template for mRNA synthesis?
A G C T U
DNA STRAND 1 19.1 26 31 23.9 0
DNA STRAND 2 24.2 30.8 25.7 19.3 0
m RNA 19.0 25.9 30.8 0 24.3
(1) Strand 1
(3) Both strands
(2) Strand 2
(4) Neither strand
TRANSLATION
TIFR PREVIOUS
YEAR EXAM
QUESTIONS
TRANSLATION
(TIFR 2018)
Which one of the following changes will not alter the sequence of the encoded
protein?
(1) Codon optimization
(2) Gene methylation
(3) Synonymous mutation
(4) All of the above
TRANSLATION
(TIFR 2018)
Which of the following mRNA molecules would form the most stable stem-loop
structure?
(1) 5‘…GGCUU…UUCGG...3’
(2) 5’…GGCUU... AAGCC....3’
(3) 5‘…GGCUU...GGCUU...3’
(4) 5‘…GGCUU...CCGAA...3’
TRANSLATION
(TIFR 2021)
Making a protein from a gene in eukaryotic cells Involves:
(1) First, transport of mRNA, then its processing. then transcription, then translation
(2) First, transcription of the RNA, then its transport, then processing, then translation
(3) First, translation of the RNA, then its transport, then its processing, then
transcription
(4) First, transcription of RNA, then its processing, then its transport, then translation
TRANSLATION
(TIFR 2021)
Making a protein from a gene in eukaryotic cells Involves:
(1) First, transport of mRNA, then its processing. then transcription, then translation
(2) First, transcription of the RNA, then its transport, then processing, then translation
(3) First, translation of the RNA, then its transport, then its processing, then
transcription
(4) First, transcription of RNA, then its processing, then its transport, then translation
ANS- 4
THE GENETIC CODE
TIFR PREVIOUS YEAR EXAM QUESTIONS
a presentation about all things DNA.
THE GENETIC CODE
(TIFR 2010)
A messenger RNA is 336 bases long including the initiation and terminator codons.
The number of amino acids in the polypeptide translated from this
(1)110
(2) 333
(3) 111
(4) 600
THE GENETIC CODE
(TIFR 2013)
How many different transfer RNA molecules are possible in a hypothetical organism
where the ribosome detects a quadruplet instead of a triplet codon including the start
and stop codon?
(1) 64
(2) 128
(3) 20
(4) 256
THE GENETIC CODE
(TIFR 2020)
In a new species of bacteria, tRNA anticodons are recognized by two nucleotide
bases instead of three. What would be the number of amino acids and the number of
peptide bonds, respectively, of a polypeptide encoded by a 120 nucleotide long
coding DNA sequence which includes a two-base stop codon?
(1) 58, 58
(2) 59,58
(3) 60, 59
(4) 61, 60
LAC,TRP OPERON
TIFR PREVIOUS YEAR EXAM QUESTIONS
LAC,TRP OPERON
(TIFR 2010)
A mutation occurs in the lac repressor protein such that it
can no longer bind to lactose. What effect will this
mutation have on expression of proteins of the lac operon
in the presence of lactose?
(1) It will have no effect since lactose shuts off the operon.
(2) It will decrease expression of the lac operon proteins.
(3) It will increase the expression of lac operon proteins.
(4) It will have no effect since the operon is modulated by
another protein.
REGULATORY
RnA
PREVIOUS YEAR
EXAM QUESTIONS
REGULATORY RNAs
(TIFR 2013)
Which of the following DOES NOT contribute to protein diversity (variants of the same
protein)?
(1) RNA editing
(2) RNA splicing
(3) RNA interference
(4) Alternative initiation of translation
