BP305P.

PHARMACEUTICAL ORGANIC CHEMISTRY -II (Practical)

4 Hrs/week
1. Experiments involving laboratory techniques:
• Recrystallization.
• Steam distillation.
2. Determination of following oil values (including standardization of reagents):
• Acid value.
• Saponification value.
• Iodine value.
3. Preparation of compounds
• Benzanilide/Phenyl benzoate/Acetanilide from Aniline/Phenol/Aniline by acylation
reaction.
• 2,4,6-tribromo aniline/para bromo acetanilide from Aniline.
• Acetanilide by halogenation (Bromination) reaction.
• 5-nitrosalicylic acid/meta di-nitrobenzene from salicylic acid/ nitro benzene
by nitration reaction.
• Benzoic acid from benzyl chloride by oxidation reaction.
• Benzoic acid/ Salicylic acid from alkyl benzoate/ alkyl salicylate by hydrolysis
reaction.
• 1-Phenyl azo-2-napthol from Aniline by diazotization and coupling reactions.
• Benzil from benzoin by oxidation reaction.
• Dibenzal acetone from benzaldehyde by Claisen-Schmidt reaction.
• Cinnammic acid from benzaldehyde by Perkin reaction.
• p-Iodo benzoic acid from p-amino benzoic acid.

Recommended Books (Latest Editions)

• Organic Chemistry by Morrison R.T., Boyd R.N. and Bhattacharjee, S.K. Dorling
Kindersley (India) Pvt. Ltd. (Pearson Education Ltd.), New Delhi.
• Organic Chemistry by Jonathan Clayden, Nick G. S. Warren. Oxford University Press,
Oxford.
• Organic Chemistry by G. Marc Loudon, Oxford University Press, Oxford.
• Organic Chemistry by Francis A. Carey and Robert M. Giuliano, Tata McGraw Hill
Publishing Company Ltd., New Delhi.
• Strategic Applications of Named Reactions in Organic Chemistry by Laszlo Kurti and
Barbara Czako, Elsevier Academic Press.
• Organic Chemistry by I.L. Finar, Volume-I, Pearson Education Ltd, New Delhi.

Evaluation Scheme Bachelor of Pharmacy I, II, III & IV Year syllabus 2019-2020 Page 48
• Elementary Practical Organic Chemistry by Vogel A.I., Dorling Kindersley (India) Pvt.
Ltd. (Pearson Education Ltd.), New Delhi.
• Practical Organic Chemistry by Mann F.G, and Saunders B.C., Dorling Kindersley (India)
Pvt. Ltd. (Pearson Education Ltd.), New Delhi.
• Introduction to Organic Laboratory Techniques by Pavia, Lampman and Kriz, Cengage
Learning, Delhi.
• Reaction and Reaction Mechanism by Ahluwalia/Chatwal, Narosa Publishing House, New
Delhi.
• A Guidebook to Mechanism in Organic Chemistry by Sykes P., Longman Group Ltd,
London.
• Organic Chemistry by Jain M.K., Sohan Lal Nagin Chand & Co, New Delhi.
• Textbook of Organic Chemistry by P.L. Soni, Sultan Chand & sons, New Delhi.

Evaluation Scheme Bachelor of Pharmacy I, II, III & IV Year syllabus 2019-2020 Page 49
 BP306P. PHYSICAL PHARMACEUTICS – I (Practical)
4 Hrs/week

1. Determination the solubility of drug at room temperature.

2. Determination of pKa value by Half Neutralization/Henderson Hasselbalch equation.
3. Determination of Partition co- efficient of benzoic acid in benzene and water.
4. Determination of Partition co- efficient of Iodine in CCl4 and water.
5. Determination of % composition of NaCl in a solution using phenol-water system by
CST method.
6. Determination of surface tension of given liquids by drop count and drop weight method.
7. Determination of HLB number of a surfactant by saponification method.
8. Determination of Freundlich and Langmuir constants using activated char coal.
9. Determination of critical micellar concentration of surfactants.
10. Determination of stability constant and donor acceptor ratio of PABA-Caffeine complex
by solubility method.
11. Determination of stability constant and donor acceptor ratio of Cupric Glycine complex
by pH titration method.

Recommended Books: (Latest Editions)

• Physical Pharmacy by Alfred Martin, Lippincott Williams and Wilkins, USA.
• Tutorial Pharmacy by Cooper and Gunn, CBS, New Delhi.
• Pharmaceutical Calculations by Stocklosam J., Lea & Febiger, Philadelphia.
• Pharmaceutical Dosage forms: Disperse systems by Lieberman H.A, Lachman C, Volume 3,
Marcel Dekker Inc.
• Physical Pharmaceutics by Ramasamy C. and Manavalan R., PharmaMed Press, Hyderabad.
• Laboratory Manual of Physical Pharmaceutics by C.V.S. Subramanyam. J., Thimma Settee.
• Experimental Pharmaceutics by Eugene, Parott, Burgess Pub. Co., UK.
• Physical Pharmaceutics by C.V.S. Subramanyam. CBS Publication
• Textbook of Physical Pharmacy by Gaurav Jain & Roop K. Khar, Reed Elsevier India Pvt.
Ltd., New Delhi.
• Physical Pharmaceutics by Shotton E & Ridgeway K, Oxford University Press, London.
• Essentials of Physical Pharmacy by D.V. Derle, BSP Book Pvt. Ltd., Hyderabad.
• Pharmaceutics: The Design and Manufacture of Medicines by Aulton M.E, Churchill
Livingstone.

Evaluation Scheme Bachelor of Pharmacy I, II, III & IV Year syllabus 2019-2020 Page 51
 BP307P. PHARMACEUTICAL MICROBIOLOGY (Practical)

4 Hrs/week
1. Introduction and study of different equipment and processing, e.g., B.O.D. incubator,
laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator,
microscopes used in experimental microbiology.
2. Sterilization of glassware, preparation and sterilization of media.
3. Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations.
4. Staining methods- Simple, Grams staining and acid-fast staining (Demonstration with
practical).
5. Isolation of pure culture of micro-organisms by multiple streak plate technique and other
techniques.
6. Microbiological assay of antibiotics by cup plate method and other methods
7. Motility determination by Hanging drop method.
8. Sterility testing of pharmaceuticals.
9. Bacteriological analysis of water
10. Biochemical test.

Recommended Books (Latest edition)

• Pharmaceutical Microbiology by W.B. Hugo and A.D. Russel: Blackwell Scientific
Publications, Oxford London.
th
• Industrial Microbiology by Prescott and Dunn., 4 edition, CBS Publishers & Distributors,
Delhi.
• Microbiology by Pelczar and Chan Kreig, Tata McGraw Hill, New Delhi.
• Lippincott’s Illustrated Reviews-Microbiology by Harvey, Champe and Fisher, Lippincott
Williams and Wilkins, New Delhi.
• Principles and Practices of Contamination Control and Cleanrooms by C.K. Moorthy, Pharma
Book Syndicate, Hyderabad.
• Pharmaceutical Microbiology by Malcolm Harris, Balliere Tindall and Cox., The Williams &
Wilkins Co., NY.
• Fundamental Food Microbiology by Bibek Ray and Arun Bhunia, CRC Press, NY.
• Industrial Microbiology by Rose, Butterworths, USA.
• Fundamentals of Microbiology by Frobisher M., Hinsdill et al., 9th ed., Japan.
• Cooper and Gunn’s Tutorial Pharmacy, CBS Publisher and Distribution.
• Microbial Technology by Peppler, Academic Press.
• I.P., B.P., U.S.P. - latest editions.
• Ananthnarayan and Paniker’s Textbook of Microbiology, edited by C.K.J. Paniker, Orient-

Evaluation Scheme Bachelor of Pharmacy I, II, III & IV Year syllabus 2019-2020 Page 54
 Longman, Hyderabad.
• Fundamentals of Microbiology by Edward, Benjamin Cummings, USA.

• Pharmaceutical Microbiology by N.K. Jain, Vallabh Prakashan, Delhi.

• Bergey’s Manual of Systematic Bacteriology, Williams and Wilkins, Philadelphia.
• Disinfection and Sterilization- Theory and Practice, General and Industrial Chemistry Series
by Sykes G., E & F.N. Spon Ltd., London.
• General Microbiology by Stanier R.Y., Ingraham, J.L., Wheelis M.L., Painter P.R., Macmillan
Press Limited, London.
• Microbiology: An Introduction by Tortora, G.J., Funke, B.R. and Case, C.L., Pearson India
Education Services Pvt. Ltd., Noida.
• Pharmaceutical Dosage Forms: Parenteral Medications by Sandeep Nema, John D. Ludwig,
Informa Healthcare.

Evaluation Scheme Bachelor of Pharmacy I, II, III & IV Year syllabus 2019-2020 Page 55
 BP308P. PHARMACEUTICAL ENGINEERING (Practical)

4 Hours/week
1. Determination of radiation constant of brass, iron, unpainted and painted glass.
2. Steam distillation – To calculate the efficiency of steam distillation.
3. To determine the overall heat transfer coefficient by heat exchanger.
4. Construction of drying curves (for calcium carbonate and starch).
5. Determination of moisture content and loss on drying.
6. Determination of humidity of air – From wet and dry bulb temperatures- use of Dew point
method.
7. Description of Construction working and application of Pharmaceutical
Machinery such as rotary tablet machine, fluidized bed coater, fluid energy mill, de
humidifier.
8. Size analysis by sieving – To evaluate size distribution of tablet granulations –
Construction of various size frequency curves including arithmetic and
logarithmic probability plots.
9. Size reduction: To verify the laws of size reduction using ball mill and determining
Kicks, Rittinger’s, Bond coefficients, power requirement and critical speed of Ball Mill.
10. Demonstration of colloid mill, planetary mixer, fluidized bed dryer, freeze dryer and
such other major equipment.
11. Factors affecting rate of filtration and evaporation (Surface area, Concentration and
Thickness/viscosity).
12. To study the effect of time on the rate of crystallization.
13. To calculate the uniformity Index for given sample by using Double Cone Blender.

Recommended Books: (Latest Editions):

• Introduction to Chemical Engineering b y Walter L. Badger & Julius Banchero, Tata
McGraw Hills, New Delhi.
• Solid Phase Extraction, Principles, Techniques and Applications by Nigel J.K.
Simpson- Latest edition.
• Pharmaceutical Engineering by K. Sambamurthy, New Age International (P) Ltd., New
Delhi.
• Unit Operation of Chemical Engineering by McCabe Smith, McGraw Hills, New Delhi.
• Pharmaceutical Engineering Principles and Practices by C.V.S Subrahmanyam et al.,
Vallabh Prakashan, Delhi.
• Remington Practice of Pharmacy by Martin, Latest edition.
• Lachman/Lieberman’s Theory and Practice of Industrial Pharmacy by Roop K. Khar, S.P.
Vyas, F.J. Ahmad and G.K. Jain, CBS Publishers & Distributers Pvt. Ltd., New Delhi.

Evaluation Scheme Bachelor of Pharmacy I, II, III & IV Year syllabus 2019-2020 Page 58
• Cooper and Gunn’s Tutorial Pharmacy edited by S.J. Carter, CBS Publishers & Distributers
Pvt. Ltd., New Delhi.
• Unit Operations by G.G. Brown, CBS Publishers & Distributers Pvt. Ltd., New Delhi.
• Perry’s Chemical Engineers’ Handbook by R.H. Perry and D.W. Green, McGraw-Hill,
USA.
• Aulton’s Pharmaceutics: The Design and Manufacture of Medicines; 3 rd edition, Churchill
Livingstone, UK.
• Bentley’s Textbook of Pharmaceutics edited by E.A. Rawlins, Reed Elsevier India Pvt.
Ltd., New Delhi.
• Pharmaceutical Process Engineering by Anthony J. Hickey and David Ganderton, Vol-112,
Drugs and Pharmaceutical Sciences, Marcel Dekker, Inc., USA.

Evaluation Scheme Bachelor of Pharmacy I, II, III & IV Year syllabus 2019-2020 Page 59