TECHNICAL DATA SHEET
TRYPTONE-SALT BROTH
DILUENT
1 INTENDED USE
Tryptone-Salt Broth is a diluent used to prepare stock solutions of food and dairy products as well as cosmetics in
preparation for microbiological analysis. It is also used to prepare serial dilutions.
2 PRINCIPLES
Tryptone assures the resuscitation of microorganisms having undergone sublethal treatments.
Sodium chloride provides an isotonic solution.
3 TYPICAL COMPOSITION
The composition can be adjusted in order to obtain optimal performance.
For 1 liter of media :
- Tryptone ...................................................................................................................... 1,0 g
- Sodium chloride .......................................................................................................... 8,5 g
pH of the ready-to-use media at 25 °C : 7,0 ± 0,2.
4 PREPARATION
• Dissolve 9,5 g of dehydrated media in 1 liter of distilled or demineralized water.
Reconstitution :
• Stir slowly until complete dissolution. 9,5 g/L
• Dispense in tubes or flasks.
• Sterilize in an autoclave at 121°C for 15 minutes. Sterilization :
• Cool the media to room temperature. 15 min at 121 °C
5 INSTRUCTIONS FOR USE
Preparation of stock solutions :
• Aseptically add X g or X mL of the product to test to a vial of media prepared as above (or using ready-to-use
media BM114 or BM135), in order to perform 1:10 dilutions.
• Mix well.
Preparation of decimal dilutions :
• Add 1 mL of stock solution to a tube containing 9 mL of medium prepared as above or to ready-to-use media
BM008.
• Mix well.
• Repeat the operation until the desired dilution is obtained.
6 QUALITY CONTROL
Dehydrated media : white powder, free-flowing and homogeneous.
Prepared media : colorless solution, limpid.
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Typical culture response after 45 minutes to 1 hour of incubation at laboratory room temperature 18-27 °C (NF EN ISO
11133) :
Microorganisms Growth
Escherichia coli WDCM 00013 ± 30 % of colonies / T0
Staphylococcus aureus WDCM 00034 ± 30 % of colonies / T0
7 STORAGE / SHELF LIFE
Dehydrated media : 2-30 °C. The expiration date is indicated on the label.
Ready-to-use media in tubes, vials or flexible bags : 2-25 °C. The expiration dates are indicated on the labels.
(*)
Prepared media : 180 days at 2-25 °C.
(*) Benchmark value determined under standard preparation conditions, following manufacturer’s instructions.
8 PACKAGING
Dehydrated media :
500 g bottle .......................................................................................................................................................... BK014HA
Ready-to-use media
50 x 9 mL tubes .................................................................................................................................................. BM00808
10 x 90 mL vials .................................................................................................................................................. BM11408
3 x 3 L flexible bags ............................................................................................................................................ BM13508
9 BIBLIOGRAPHY
ISO 14461-2. April 2005. Milk and milk products — Quality control in microbiological laboratories - Part 2:
Determination of the reliability of colony counts of parallel plates and subsequent dilution steps.
ISO 14461-1. May 2005. Milk and milk products — Quality control in microbiological laboratories - Part 1: Analyst
performance assessment for colony counts.
NF EN ISO 8199. October 2018. Water quality - General requirements and guidance for microbiological
examinations by culture.
NF EN ISO 11133. Juillet 2014. Microbiology of food, animal feed and water - Preparation, production, storage and
performance testing of culture media (Tirage 2 (2016-01-01)).
NF EN ISO 6887-1. June 2017. Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension
and decimal dilutions.
NF EN ISO 6887-2. June 2017. Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 2: Specific rules for the preparation of meat and meat products.
NF EN ISO 6887-3. June 2017. Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 3: Specific rules for the preparation of fish and fishery products.
NF EN ISO 6887-4. June 2017. Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 4: specific rules for the preparation of miscellaneous products.
NF EN ISO 6887-5. May 2020. Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 5: specific rules for the preparation of milk and milk products.
NF EN ISO 18415. August 2017. Cosmetics - Microbiology - Detection of specified and non-specified
microorganisms.
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10 ADDITIONAL INFORMATION
The information provided on the labels take precedence over the formulations or instructions described in this
document and are susceptible to modification at any time, without warning.
Document code : TRYPTONE SALT_ENv17
Creation date : 01-2003
Updated : 03-2023
Origin of revision : General revision.
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