Aim: Tests of carbohydrates, fats and proteins in the given food stuffs.
1. Carbohydrates – Starch
S.No. Experiment Observations Inference
1. Test for Fats or Oils:
Take a small sample (1mL) of the No pungent smell Fat or oil is not present in
food stuff in the test tube and add comes out the given food sample
some crystals of KHSO4 to it and
heat it gently.
2. Test for proteins:
Take a small sample (1mL) of the No colour change in Proteins are absent in the
food stuff add about 1 mL of the solution is given food stuff
ninhydrin solution to it. observed.
3. Test for carbohydrates:
a. Molisch Test:
Take a small sample (1mL) of the A red violet ring Carbohydrates are present
food stuff and add about 1 mL of appears at the in the given food stuff
Molisch reagent to it. junction of the two
liquids
b. Iodine Test:
Take a small sample (1mL) of the A blue black Carbohydrate (Starch) is
food stuff and add about 1 mL of colouration appears present in the given food
Iodine solution to it stuff
2. Carbohydrates – Glucose
S.No. Experiment Observations Inference
1. Test for Fats or Oils:
Take a small sample (1mL) of the No pungent smell Fat or oil is not present in
food stuff in the test tube and add comes out the given food sample
some crystals of KHSO4 to it and
heat it gently.
2. Test for proteins:
Take a small sample (1mL) of the No colour change in Proteins are absent in the
food stuff add about 1 mL of the solution is given food stuff
ninhydrin solution to it. observed.
3. Test for carbohydrates:
a. Iodine Test:
Take a small sample (1mL) of the No change in colour Carbohydrate (Starch) is
food stuff and add about 1 mL of absent in the given food
Iodine solution to it stuff
b. Molisch Test:
Take a small sample (1mL) of the A red violet ring Glucose may be present in
food stuff and add about 1 mL of appears at the the given food stuff
Molisch reagent to it. junction of the two
liquids
c. Tollen’s Test:
Take a small sample (1mL) of the A shining silver Glucose is present in the
food stuff and add about 1 mL of mirror appears in the given food stuff
Tollen’s reagent to it and heat the test tube
test tube in a water bath for about 10
minutes
d. Fehling’s Test:
Take a small sample (1mL) of the Red brown Glucose is present in the
food stuff and add about 1 mL of precipitate appears given food stuff
each of the Fehling’s A and the in the test tube
Fehling’s B solution to it and heat
the test tube in a water bath for
about 10 minutes
3. Protiens
S.No. Experiment Observations Inference
1. Test for Fats or Oils:
Take a small sample (1mL) of the No pungent smell Fat or oil is not present in
food stuff in the test tube and add comes out the given food sample
some crystals of KHSO4 to it and
heat it gently.
2. Test for carbohydrates:
a. Molisch Test:
Take a small sample (1mL) of the No change in colour Carbohydrates are absent in
food stuff and add about 1 mL of the given food stuff
Molisch reagent to it.
b. Iodine Test:
Take a small sample (1mL) of the No change in colour Carbohydrate (Starch) is
food stuff and add about 1 mL of absent in the given food
Iodine solution to it stuff
3. Test for proteins:
a. Ninhydrin Test
Take a small sample (1mL) of the Intense blue Proteins are present in the
food stuff add about 1 mL of colouration in the given food stuff
ninhydrin solution to it and boil the solution is observed.
contents.
b. Biuret Test
Take a small sample (1mL) of the Bluish violet Proteins are present in the
food stuff add about 1-2 mL of colouration in the given food stuff
NaOH aq. solution to it and then add solution is observed.
about 1 mL of the 1% CuSO4
solution to it. Warm the solution for
about five minutes
c. Xanthoproteic Test
Take a small sample (1mL) of the Yellow colouration in Proteins are present in the
food stuff add about 1-2 mL of the solution is given food stuff
HNO3 conc. aq. solution to it and observed.
then heat the solution.
4. Fats or Oils
S.No. Experiment Observations Inference
1. Test for proteins:
Take a small sample (1mL) of the No colour change in Proteins are absent in the
food stuff add about 1 mL of the solution is given food stuff
ninhydrin solution to it. observed.
2. Test for carbohydrates:
a. Molisch Test:
Take a small sample (1mL) of the No change in the Carbohydrates are absent in
food stuff and add about 1 mL of colour of the solution the given food stuff
Molisch reagent to it. is observed
b. Iodine Test:
Take a small sample (1mL) of the
food stuff and add about 1 mL of No change in the Carbohydrates are absent in
Iodine solution to it colour of the solution the given food stuff
is observed
3. Test for Fats or Oils:
a. Acrolein Test
Take a small sample (1mL) of the A pungent irritating Fat or oil is present in the
food stuff in the test tube and add smell comes out given food sample
some crystals of KHSO4 to it and
heat it gently.
b. Baudouin Test:
Take a small sample (1mL) of the
food stuff and add about 5 mL of A rose red colour of Given fat sample vanaspati
conc. HCl solution to it and 2-3 the solution is ghee as adultrant in the desi
drops of 2% furfural solution to it observed ghee
and keep it aside for 5-10 minutes.
c. Translucency Test:
Press a little of the substance in the On unfolding the Fat or oil is present in the
folds of thee filter paper. filter paper, the given food sample
appearance of the
translucent or greasy
spot on the filter
paper is observed
Result: The given food samples have carbohydrates, proteins and fats in them as nutrients.
