Practice 8 Sucrose-Refractometry
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ScribdTranslationsPractice 8 Sucrose-Refractometry
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ScribdTranslationsPRACTICE # 8.
DETERMINATION OF SUGARS BY
REFRACTOMETRY
8.1 OBJECTIVE
Build a graph of refractive index vs concentration for the sucrose-water system.
quantify the sugar present in a sample.
8.2 GENERAL INFORMATION
The refractive index measures the refraction of light through a solution. It is used to
check the purity of products such as: milk, oils, and fats, also to determine the
quality, since a variation in the refractive index indicates a change in the substance.
This method is also used to assess the maturity level of fruits and vegetables for their
harvest, as it does not indicate the content of soluble solids (sugars) present in them.
The concentration of sucrose is expressed in degrees Brix.
In products such as juices and jams, the presence of other solid substances influences the
refraction of light. However, the refractive index and the brix degree are sufficient to
determine the soluble solids content in the product.
Refraction is the deviation experienced by light rays when passing from one medium to another.
transparent dedensitydetermined to another whose density is different from the previous one. Although
this phenomenon generally occurs when passing from one medium to another, there is a case in which
this step does not imply refraction, which is when the incidence occurs perpendicularly to the
separation surface of bothmeans. Refraction is fundamental for the explanation of the
processeswhat he/she/they experiencesthelightin prisms and lenses of all kinds. While light propagates
with different speeds depending on the density of the medium through which it does so (how much
the greater the density of this, the slower the propagation of light), the intensity of the
the phenomenon of refraction depends on the degree of variation of thespeedof propagation
the greater this is, the greater the refraction experienced by the ray, and consequently
the greater it islto be able toof refraction of the medium.
The phenomenon of refraction is based onthechangeof speed that is experiencedtheradiation
electromagnetic when passing from one medium to another, as a consequence of itsinteractionwith the
atoms and molecules of the other medium. This change in velocity manifests itself in a variation in
theaddressof propagation.
When a ray of light passes obliquely from one medium to another of different density, its direction
It changes when crossing the surface that separates them. This is called refraction.
α INCIDENCE ANGLE
DIVIDING SURFACE
β ANGLE OF REFRACTION
The angle between the ray in the first medium and the perpendicular to the dividing surface is called
angle of incidence (α), meanwhile, the corresponding angle between the second medium is
denotes the angle of refraction (β). The sine of α and the sine of β are directly proportional to
the speeds of light in the two media.
REFRACTION SYSTEM DESCRIPTION
It is an analytical technique that consists of measuring the refractive index of a liquid in order to
to investigate its composition whether it is a solution or its purity if it is a single compound.
The continuous measurement in a chromatographic column can indicate the composition or purity of the
eluate.
.
8.3 MATERIALS AND REAGENTS
1 Gradilla
14 Test tubes
1 Spatula
10 ml pipettes
2 Pasteur pipettes with bulb
2 beakers of 250 ml
1 ABbe Refractometer
8.4 PROCEDURE
Starting from pure sucrose and water, a series of stock solutions with the concentrations is prepared.
The following: 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 % by weight of sucrose.
10 ml of each type is prepared and placed in capped test tubes, keeping them secure.
closed.
Determine the refractive index of each standard solution and also of the problem sample (juice.
natural and/or artificial orange, cola, mineral water
8.5 RESULTS AND CONCLUSIONS
PIPE % SACAROSA REFRACTION INDEX
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5
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8
9
10
SHOW PROBLEM
