Staining of Microorganism QUIZ
I. Matching Type 1
Match Column A with Column B based on the staining techniques and their color reactions.
Column A
1. Dieterle method
2. Toluidine blue
3. Warthin-Starry method (spirochetes)
4. Cresyl violet acetate method
Column B
A. Dark brown to black
B. Blue-violet
C. Dark blue against blue BG
D. Black
II. Matching Type 2
Match Column A with Column B based on the microorganisms and the staining reactions.
Column A
1. Toluidine blue
2. Dieterle method
3. Gram (+) bacteria
4. Gram (-) bacteria
5. RBCs in Gram-Twort stain
6. Orcein method
Column B
A. H. pylori
B. L. pneumophila & spirochetes
C. Blue-black
D. Brown-black
E. Green
F. Red
III. Multiple Choice
Instructions:
Read each question carefully and choose the best answer from the options provided.
1. In this staining technique, an acidic, anionic dye is used to change the color of the
background, not the cells, causing the cells to stand out.
A. Simple Staining
B. Negative Staining
C. Differential Staining
D. Gram Stain
2. It is a procedure that takes advantage of differences in the physical and chemical
properties of different groups of bacteria.
A. Simple Staining
B. Negative Staining
C. Differential Staining
D. Gram Stain
3. The most commonly used differential stain is the ___________, which divides bacteria
into two groups, those which are Gram-positive and those which are Gram-negative.
A. Simple Staining
B. Negative Staining
C. Differential Staining
D. Gram Stain
4. Are single dyes used to stain the organism and it has limited clinical application.
A. Simple Staining
B. Negative Staining
C. Differential Staining
D. Gram Stain
5. It have a thicker cross-linked wall of peptidoglycan and are not lipid-rich.
A. Gram-positive bacteria
B. Gram-negative bacteria
C. Gram Stain
D. Simple Staining
6. It has a very little peptidoglycan, but do have an outer layer of lipopolysaccharide, and
they have greater lipid content in their cell walls than Gram-positive cells.
A. Gram-positive bacteria
B. Gram-negative bacteria
C. Gram Stain
D. Simple Staining
7. The ____________ stain is a differential stain which distinguishes organisms with waxy
cell walls that can resist decolorization with acid alcohol.
A. Simple Staining
B. Negative Staining
C. Acid Fast Stain
D. Gram Stain
8. The _____________ method is a specific way to identify mycobacteria, which are not
readily demonstrated by other methods, including Gram stain.
A. Simple Staining
B. Fite carbol-fuchsin method
C. Microwavel Auramine-Rhodamine Fluorescent Technique
D. Gram Stain
9. This is an extremely sensitive and highly specific method for mycobacteria.
A. Simple Staining
B. Fite carbol-fuchsin method
C. Microwavel Auramine-Rhodamine Fluorescent Technique
D. Gram Stain
10. ______________ is the organism strongly implicated as a cause of chronic gastritis.
A. Helicobacter pylori
B. Fite carbol-fuchsin method
C. Mycobacteria
D. Bacteria
Answer Keys:
Matching Type 1
1. Dieterle method → A. Dark brown to black
2. Toluidine blue → C. Dark blue against blue BG
3. Warthin-Starry method (spirochetes) → D. Black
4. Cresyl violet acetate method → B. Blue-violet
Matching Type 2
1. Toluidine blue → A. H. pylori
2. Dieterle method → B. L. pneumophila & spirochetes
3. Gram (+) bacteria → C. Blue-black
4. Gram (-) bacteria → F. Red
5. RBCs in Gram-Twort stain → E. Green
6. Orcein method → D. Brown-black
Multiple Choice
1. B
2. C
3. D
4. A
5. A
6. B
7. C
8. B
9. C
10. A
