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MBTE3560 Lab Result - gp6

The document outlines a procedure for transforming E. coli using PCR products generated from mutagenic primers and subsequent DpnI digestion. It includes details about controls, such as a positive control with a GFP plasmid and the analysis of the PCR product. Additionally, it provides specific sequences related to mutated amino acids for further experimentation.

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63 views1 page

MBTE3560 Lab Result - gp6

The document outlines a procedure for transforming E. coli using PCR products generated from mutagenic primers and subsequent DpnI digestion. It includes details about controls, such as a positive control with a GFP plasmid and the analysis of the PCR product. Additionally, it provides specific sequences related to mutated amino acids for further experimentation.

Uploaded by

cvpbtthfd8
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Transformation

Group 6 PCR using mutagenic DpnI digestion of results of controls


Transformation
primers PCR product
(Annealing temperature: 65°C) to E. coli

Positive control
1 kb plus Ladder

(GFP plasmid)
Your Mutant PCR
1 kb plus Ladder
Your Mutant PCR

GFP Plasmid
E223G
E223G

E222G
[Link]

Your Mutant
[Link]/order/catalog/ Analyze the Check the

Design
product/SM1331
PCR product digested DNAs

Water
Gp 6 6: mutated a.a. 68-S GTGACCACCCTGACCTACGTCGTGCAGTGCTTCAGC
6: mutated a.a. 68-
GCTGAAGCACTGCACGACGTAGGTCAGGGTGGTCAC
AS

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