Eye Contour Balance Moisturising Protection Brightness Exfoliators

contour des yeux Equilibre Hydratant Protection Teint Exfoliant

EYElid lifter
under-eye rejuvenator
MYRALYS
 SKIN ISSUE STUDIED BY GREENTECH

MYRA AGING OF THE UPPER &

LOWER EYE CONTOURS

LYS
In 2022, facial beauty continues its metamorphosis. As a direct consequence of the covid-19
pandemic, the relationship with oneself and with others has been radically transformed, as have the
beauty rituals of women and men around the world. With the mask concealing the lower part of the
face, eyes have become the focus of attention more than ever. Playing a preponderant role in our
perception of beauty (of ourselves and of others), more than any other component of the face, it is
also in them that we look for signs of emotions, signs of essential non-verbal communication.

In 2022, make-up and eye care are being reborn. And sometimes even hybridize, in a desire to meet
the needs of women and men who want to simplify their beauty rituals while preserving quality,
safety, effectiveness and durability.

Aware of the impact of eye contour aging on the perception of oneself and others, GREENTECH
R&D identified gentiopicroside as a potent natural solution to rejuvenate the upper and lower eye
contours and designed MYRALYS, a COSMOS-certified natural active ingredient.

LIFTS DROOPY UPPER EYELIDS

LESS ROUGHNESS, LESS FOLDS

BoostS REDUCES COUNTERACTS

ACTIONS antioxidant response,
and reinforces dermal matrix
(collagen I)
inflammation & angiogenesis
while activating lymphatic
function
UV-induced glycation
and damages to elastic fibers,
stimulates detoxifying glyoxalase-1

REJUVENATES UNDER-EYE CONTOUR

REDUCES DARK CIRCLES, EYE BAGS AND TEAR TROUGH APPEARANCE

molecules
- HIGHLY TITRATED IN GENTIOPICROSIDE
≥30% /DRY MATTER
Biologically active iridoid glycoside

SOURCING
ORGANIC WILD YELLOW GENTIAN ROOTS
SUSTAINABLY HARVESTED IN FRANCE BY GENTIAN-PICKERS
TRAINED IN GOOD HARVESTING PRACTICES

Gentian roots are strictly selected by GREENTECH scientists based

on their gentiopicroside content in order to achieve a highly titrated
extract thanks to an advanced purification process.

- POSITIVE AGING - EYE CARE PRODUCTS

COSMETIC USES -
-
-
EYELID-LIFTING / PLUMPING UNDER-EYE CARE
EYE CARE PRODUCTS (ANTI-DARK CIRCLES, ANTI-EYE BAGS)
MAKE-UP FOR EYE CONTOUR
 GREENTECH INNOVATION
TOTAL EYE CONTOUR REJUVENATION
UPPER & LOWER CONTOURS

MULTIPATHWAY APPROACH
6 INTERCONNECTED PATHWAYS

HIGHLY PURIFIED GENTIOPICROSIDE

FROM SUSTAINABLY HARVESTED ORGANIC GENTIAN ROOTS

MULTIPATHWAY APPROACH
TO REDUCE AGING OF EYE CONTOURS
Skin of eye contours including eyelids has unique characteristics in relation to the complex anatomy and functions of eye area
(such as translucent thin skin, sparse fat tissue, significant vasculature, numerous muscles to realize countless daily blinks…).
Combined with a high level of exposure to environmental stressors, these characteristics may explain why eye area is the first to
be marked by signs of aging.

UPPER EYELID AGING

Oxidative stress

Dermal matrix aging Glycation

UPPER CONTOUR

LOWER CONTOUR

TEAR TROUGH
Oxidative stress UNDER-EYE BAGS
Dermal matrix aging Glycation Inflammation

Poor lymphatic function

DARK CIRCLES Dermal matrix aging Glycation

Inflammation

Angiogenesis

To treat all the signs of aging of the upper and lower eye contours, GREENTECH R&D turned its attention to gentiopicroside
from Gentiana lutea, as it was described to have a potent cytoprotective effect on cells damaged by glycation, and biological
activities leading to a reduction of oxidative stress, inflammation and vascular disorders.
(Kołodziejczak and Rotsztejn, 2020; Zhang et al., 2019)
 Stimulated conditions: VEGF – 100 ng/ml

PROVEN Control Suramin

EFFICACY 100 µM
IN VITRO/EX VIVO ANALYSIS

1. EFFECT ON ANTIOXIDANT & INFLAMMATORY PATHWAYS

(1)(2)
NHDF, ± MYRALYS 0.025%. Gene expression by RT-qPCR.

02_Pseudotubes
MV200702_Pseudotubes
NRF2(1) CXCL8/IL8(2)

+21% -20%
otubes MYRALYS MODULATES ANTIOXIDANT & INFLAMMATION RESPONSES
TO REDUCE SKIN AGING/AGE FORMATION AND EYE CONTOUR ISSUES
T+3 R+3

2. EFFECT ON LYMPHATIC FUNCTION & ANGIOGENESIS

Stimulated
[Link]:
MODULATES VEGF
THE– 100 Stimulated
ng/ml OF
RELEASE conditions:
KEYStimulated
VASCULAR VEGF – VEGF
conditions: 100 ng/ml
ENDOTHELIAL –GROWTH
100 ng/mlFACTORS
NHDF, ± MYRALYS 0.03% (VEGF-A) or 0.3% (VEGF-C), ELISA

Stimulated conditions: VEGF – 100 ng/ml

Non-stimulated control control
GELC2008P1-Z50 Control Suramin
Non-stimulated
VEGF-C +48%** DRAINING Control
EFFECT VEGF-A -15% ** ANTI-ANGIOGENESIS
Suramin
**p<0.01 vs control
ulated control 0.1% Control 0.3% Suramin 100 µM 100 µM
2.2. INHIBITS VEGF-INDUCED ANGIOGENESIS (PSEUDO-TUBE FORMATION)
Co-culture NHDF-HMVEC-d, ± VEGF-A (100 [Link]-1) ± MYRALYS 0.3%. VWF immunostaining 100
( ) µM
+ PROANGIOGENIC VEGF-A

CONTROL STIMULATED CONTROL MYRALYS 0.3%

Pseudo-tubes area ( mm2)

1
+419%** MYRALYS INHIBITS ANGIOGENESIS
0.8
& ACTS TO THE RESOLUTION
0.6
OF DARK CIRCLES & EYE BAGS
0.4

0.2 -43%$
0
**p<0.01 $
p<0.05
T-3 12+1 T+3 11+2 R+3
T-3 vs control T+3 vs stimulated control R+3

T-3 T+3 R+3

3. EFFECT ON DERMAL MATRIX PRODUCTION
Stimulated conditions:
Stimulated
NHDF from eyelid skin of 55 yo woman, ± MYRALYS 0.25%, ELISA.
VEGF – VEGF
conditions: 100 ng/ml
– 100 ng/ml
Stimulated conditions: VEGF – 100 ng/ml
BOOSTS COLLAGEN I SYNTHESIS +76%* TO FIRM SKIN & LIMIT SAGGING
GELC2008P1-Z50
GELC2008P1-Z50
OF UPPER & LOWER EYELIDS
ON EYELID-DERIVED FIBROBLASTS *p<0.05 vs control
GELC2008P1-Z50
0.03% 0.03% 0.1% 0.1% 0.3% 0.3%
0.03% 4. EFFECT ON
0.1%GLYCATION & DERMAL
0.3% ELASTIC FIBERS
Skin explants from 42 yo woman, topical application of MYRALYS 0.5% or control solution, during 24h. ±UVA stress ([Link]-2). GLO-1, CML or elastin immunostainings.

4.1. STIMULATES DETOXIFYING GLYOXALASE SYSTEM : GLYOXALASE-1 (GLO-1)

UV

MYRALYS 0.5% STIMULATED CONTROL MYRALYS 0.5% **p<0.01 vs control

AMPLIFYING
EFFECT
$$$
p<0.001
In total skin +19% **
+11% +35% $$$
vs UV-stimulated control

Normal reaction to UV stress

MYRALYS BOOSTS SKIN DETOXIFYING CAPACITY TO LIMIT FORMATION OF AGEs

13+1 12+1 11+2

4.2. REDUCES
13+1 FORMATION OF AGEs IN SKIN
12+1 : Nε-(CARBOXYMETHYL)-LYSINE
11+2 (CML )
13+1 12+1 11+2
AGEs: Advanced Glycation End-Products
UV
CONTROL MYRALYS 0.5% STIMULATED CONTROL MYRALYS 0.5%

Epidermis
MYRALYS STRONGLY
REDUCES CML:
TOTAL NEUTRALIZATION
Dermis
IN UV-INDUCED CONDITION

-11% +27%** -111% **p<0.01 vs control

INHIBITORY $$$
In total skin EFFECT $$$
p<0.001 vs UV-stimulated control

4.3. PROTECTS ELASTIC FIBER NETWORK : 41% PROTECTION VS UV-INDUCED

ELASTIN DEGRADATION
p=0.08 vs UV-stimulated control
PROVEN
EFFICACY
CLINICAL STUDY - IN VIVO
• 44 volunteers in 2 groups with specific criteria & evaluations.
- Group 1: 22 volunteers, 43-64 yo (mean: 52 yo) including 17 women + 5 men. Upper eyelids with folds and visible dark circles.
- Group 2: 22 volunteers, 47-64 yo (mean: 56 yo) including 15 women + 7 men. Visible under-eye bags and visible tear trough.
• MYRALYS 0.5% versus Placebo (gel). Randomized Split-face application 2x/day, 28 days.
• Measurements at D0, D14 and D28:
- Upper eyelids, using DermaTOP ® (Average skin roughness-Ra & Eyelid skin folds-Rz) and Dark circles (redness (a*) using Spectrophotometer ®).
- Under eye-bags (volume) & Tear trough (Roughness - Ra, Rz) and mean depth, using DermaTOP ® (fringe projection)

SMOOTHES EYELID SKIN LIFTS EYELID SKIN FOLDS

D0

Heights

-22%$$
Fold height (Rz)
D14 D14 vs D0
Hollows
$$ p<0.01vs D0
p=0.08 vs placebo

Smoothing effect on periorbital wrinkles

UPPER CONTOUR
-18%
Roughness
$
(Ra)
REJUVENATION
D14 vs D0

$ p<0.05 vs D0

TOTAL EYE REJUVENATION

FAST & VISIBLE RESULTS IN JUST 14 DAYS

LOWER CONTOUR
REJUVENATION

SMOOTHES & PLUMPS REDUCES BRIGHTENS

TEAR TROUGH EYE BAGS DARK CIRCLES

-14%* -18%* -14%$$

Mean Depth Volume Redness (a*)
D14 vs D0 D14 vs D0 D14 vs D0

*p<0.05 vs placebo at D14 *p<0.05 vs placebo at D14 $$ p<0.01 vs D0

(p<0.05 vs placebo)

Placebo MYRALYS 0.5%

D0
D14

D0 D14 D28

Placebo Placebo

D0
D14

MYRALYS 0.5%
D0 D14 D28
(Dark circle color representation
MYRALYS 0.5% based on L*a*b* values of a volunteer)
ORGANIC MYRALYS

TECHNICAL INFORMATION
FORMULATION Caution for use:
Concentration for use: 0.5% Add at any time for cold processes and during cooling (30-40°C) for hot
pH for use: 4.0 - 8.0  processes. Formulation guidelines and stability studies available on demand.

TECHNICAL DATA
Characteristics
Organoleptic Appearance: liquid
Color: Amber yellow to brown
Solubility Soluble in water, in glycols, in alcohol (with pH<6)

Storage
Keep in a dark place, in the original packaging, at an ambient temperature between 15° and 25 °C.

Tests of tolerance
• Cutaneous irritation (at 3%) : Very good skin compatibility (Patch test)
• Sensitization (at 3%) : Very good skin compatibility (HRIPT)
• Eye irritation (at 3%) : Moderately irritant (HETCAM)
• Eye irritation (at 2% in formula) : Slightly irritant (HETCAM)
• Phototoxicity : Not phototoxic (3T3 cells)
• Mutagenicity test : Not mutagenic (Ames test)

• INCI name: Aqua/Water, Propanediol, Gentiana Lutea Root Extract

• Preservative: None
• Authorized: China
• Certified: COSMOS
• Naturality (ISO 16128) : 100% natural origin content

Organic certified by ECOCERT GREENLIFE, according to COSMOS Standard

Warning: The information in this document are the result of studies and interpretations based in part on published or generally accepted scientific data. They are provided for information purposes in terms
of possible end applications and cannot be considered as instructions for use. We accept no responsibility concerning marketed formulas containing one or more of our products. Compounders must take
all necessary precautions and ensure that all legal and administrative formalities have been fulfilled.

Edition, March 2022

Biopôle Clermont-Limagne - 63360 St Beauzire - France Germany: info@[Link]

Phone: +33 4 73 33 99 00 - E-mail: greentech@[Link] Brazil: contato@[Link]
Web site: [Link] USA: greentechusa@[Link]