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Structural and Functional Genomics Notes

The study notes cover structural and functional genomics, detailing structural genomics' focus on determining 3D protein structures using experimental and computational methods, while functional genomics studies gene functions and interactions. Key applications include drug discovery and personalized medicine, with tools like RNA-seq and CRISPR. Future trends point towards advancements in single-cell genomics and the integration of AI/ML in genomics.

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171 views3 pages

Structural and Functional Genomics Notes

The study notes cover structural and functional genomics, detailing structural genomics' focus on determining 3D protein structures using experimental and computational methods, while functional genomics studies gene functions and interactions. Key applications include drug discovery and personalized medicine, with tools like RNA-seq and CRISPR. Future trends point towards advancements in single-cell genomics and the integration of AI/ML in genomics.

Uploaded by

Neha
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Structural and Functional Genomics - Study Notes

STRUCTURAL AND FUNCTIONAL GENOMICS: COMPREHENSIVE STUDY NOTES

STRUCTURAL GENOMICS: DETAILED STUDY NOTES

1. Introduction

- Structural genomics aims to determine 3D structures of all proteins encoded by a genome.

- It uses high-throughput experimental (X-ray, NMR) and computational (modeling, homology) methods.

- Focuses on understanding sequence-structure-function relationships.

2. Key Features

- Genome-wide coverage vs. single-protein approach in structural biology.

- Often, function is unknown when structure is solved.

- Enabled by projects like the Protein Structure Initiative (PSI).

3. Advantages and Limitations

+ Rapid structural coverage, tool/resource sharing (e.g., clones).

- Some structures lack annotation; need tools like TOPSAN for function prediction.

4. Experimental Methods

- X-ray crystallography, NMR, and Cryo-EM.

- Requires gene cloning, expression, purification, and crystallization.

5. Computational Modeling

- Ab initio (e.g., Rosetta), homology modeling, threading.

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Structural and Functional Genomics - Study Notes

- Homology modeling is accurate at >50% sequence identity.

6. Applications

- Drug discovery, novel fold identification, protein engineering.

7. Key Examples

- Thermotoga maritima: Novel fold TM0449 discovered.

- Mycobacterium tuberculosis: 700+ protein targets structurally characterized.

FUNCTIONAL GENOMICS: DETAILED STUDY NOTES

1. Introduction

- Studies gene functions and interactions at a genome-wide scale.

- Connects genotype to phenotype via transcriptomics, proteomics, etc.

2. Goals

- Functional annotation, gene interaction networks, pathway mapping.

3. Tools and Techniques

- Microarrays, RNA-seq, CRISPR, ChIP-seq, Yeast Two-Hybrid, MS.

4. Applications

- Disease gene discovery, drug target identification, personalized medicine.

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Structural and Functional Genomics - Study Notes

5. Functional Annotation

- Uses Gene Ontology (GO), KEGG pathways, UniProt, and databases.

6. Future Trends

- Single-cell genomics, AI/ML in genomics, integrated multi-omics.

7. Comparison Table

Functional Genomics vs. Structural Genomics:

- Functional: Expression, interaction | Structural: 3D structure

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