SID No : 96315309 Patient ID : 9600314569
Branch : Coimbatore
Mr. PARTHASARATHY
Age / Sex: 29 Y / Male Collected Date : 06/12/2021
06/12 / 9:47
Ref. By : Dr. PRIYA DARSHINI M.B.B.S. ,M.D. , Received Date : 15/12/2021
15/12 / 10:29
Reported Date : 15/12/2021 / 16:16
Final Test Report Page 1 of 1
MOLECULAR BIOLOGY
SARS-CoV- 2 (COVID-19) RT-PCR
Specimen Combined swab (Nasopharyngeal & Throat)
SARS - CoV-2 Negative
ICMR approved ID: BIOLCT for COVID-19 testing.
Method: Real-time Reverse Transcription Polymerase Chain Reaction (RT-PCR).
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV CoV-2) is a positive-sense, single-stranded
stranded RNA virus that causes Coronavirus disease 2019 (COVID-19).
(COVID SARS-CoV-2
transmission occurs primarily via respiratory droplets. SARS
SARS-CoV-2 is likely to be at thee highest concentrations in the nasopharynx during the first 3 to 5 days of symptomatic
illness. As the disease progresses, the viral load tends to decrease in the upper respiratory tract, at which point lower res
respiratory
piratory tract specimens (eg, sputum, tracheal
trach aspirate,
bronchoalveolar fluid) would be more likely to have detectable SARS-CoV-2.
Interpretation Guidance:
A "Negative" result indicates that SARS-CoV CoV-2 is not present in the patient's specimen. However, this result may be influenced by the stage of the
infection, quality, and type of the specimen collected for testing. Result should be correlated with patient’s history and clinical cl presentation. A single
negative test result, particularly if this is from an upper respiratory tract specimen, does not exclude infection. Importantly, combination of real-time
real RT-
PCR and clinical features especially CT image could facilitate disease management.
A "Positive" result indicates that severe acute respiratory syndrome coronavirus 2 (SARS
(SARS-CoV-2) 2) RNA is present and suggests the diagnosis of coronavirus
disease 2019 (COVID-19). Test result should always be considered in the context of patient's clinical history, physical phys examination, and epidemiologic
exposures when making the final diagnosis. A positive alternate pathogen does not necessarily rule out either, as little is yet known about the role of
coinfections.
The above qualitative result of COVID-19 19 using RT
RT-PCR method is based on the threshold cycle (Ct) value on detection of highly specific and exclusive
detection of the SARS-CoV-2 2 genes in respiratory samples along with simultaneous detection of a universally expressed human gene to exclude false
negative results.
ts. The sensitivity of the assay is dependent on the timing of the specimen collection (in relation to symptom onset), quality,
qualit and type of the
specimen submitted for testing.
Real-Time RT-PCR
PCR Diagnostic kits have been designed to minimize the likelihood o off false test results. Still, there is a very small chance that this test result
can vary due to stray viral RNA,, very low viral load, stage of infection, decreased viral shedding at the anatomic sampling site or improper sampling of the
nasopharyngeal swab in some people with COVID COVID-19.
Results from two different lab are not comparable as the test depends on several factors including the viral load during sample samp collection procedure,
timeline in the course/stage of disease, epidemiological prevalence of the disease, performance of the kit and type of target genes, etc.
The above result should not be used for any thesis or presentations or for Publication in any Journal without the prior permission
permi of the Director General,
ICMR.
As per ICMR advisory, numerical Ct values is useful for laboratory result interpretation only which may vary due to several factors and hence not
recommended for determining infectiousness of COVID-19 patients (viral load) and deciding patient management protocols
The RT-PCR test presently being conducted is qualitative. So numerical cycle threshold (Ct)values for determining infectiousness of COVID-19 patients and
deciding patient management protocols is not recommended (ICMR Dt 05/08/2020). Ct values shall be provided only to physicians verbally, on request
Verified By Dr.T.Amalarajasundari
Mr.M.Balachandar DEPARTMENT INCHARGE
