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How To Use Autopipette

This document provides instructions for using a Microautopipette effectively, emphasizing preparation, liquid drawing, dispensing, and post-use disposal. Key practices include selecting the appropriate micropipette and tip, maintaining proper angles during liquid handling, and ensuring safe disposal of used tips. Additional tips highlight the importance of avoiding contamination and handling the pipette delicately.

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abhinandan800569
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240 views1 page

How To Use Autopipette

This document provides instructions for using a Microautopipette effectively, emphasizing preparation, liquid drawing, dispensing, and post-use disposal. Key practices include selecting the appropriate micropipette and tip, maintaining proper angles during liquid handling, and ensuring safe disposal of used tips. Additional tips highlight the importance of avoiding contamination and handling the pipette delicately.

Uploaded by

abhinandan800569
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

To use a Microautopipette correctly and efficiently, follow these steps to ensure accuracy

and avoid contamination


Preparation
Choose a micropipette suitable for your desired volume range and attach a compatible
sterile tip securely.
Turn the volume adjustment dial until the desired volume is displayed on the micropipette.
Ensure the pipette tip is free from cracks or defects to avoid leaks.

Drawing the Liquid


Keep the pipette vertical or at a slight angle (~10°) when aspirating liquid.
Submerge the tip just below the liquid surface (2-3 mm) to prevent air bubbles.
Press the plunger to the first stop before immersing the tip.
Slowly release the plunger to aspirate the liquid without introducing air bubbles.

Dispensing the Liquid


Touch the tip to the side of the container or above the target surface at an angle.
Press the plunger to the first stop to dispense the liquid.
Press to the second stop to expel any remaining liquid completely.
Withdraw the tip from the solution while still holding the plunger down to avoid drawing
back liquid.

After Use
Use the ejector button to safely dispose of the used tip in a biohazard container.

Tips for Best Practices


Avoid turning the pipette upside down with liquid inside to prevent contamination.
Use the correct tip for better precision and avoid leakage.
Kindly use he autopipette delicately and avoid hitting it against any hard surface
Avoid playing with the plunger

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