7.75536.0005-xxxxxxxxxx msp.
February 2020
1.01812.0001 7. Procedure
Reagent SO4-1 0.50 ml Pipette into a test tube.
Spectroquant® Pretreated sample 10 ml Add with pipette and mix.
SO42-
(20 - 40 °C)
Sulfate Test Reagent SO4-2 1 level green
microspoon (in
Add and shake vigorously until the
reagent is completely dissolved.
the cap of the
SO4-2 bottle)
Leave to stand for exactly 2 min (reaction time), then fill the measure-
1. Method ment sample into the cell, and measure in the photometer.
Sulfate ions react with barium ions to form slightly soluble barium sulfate.
The resulting turbidity is measured in the photometer (turbidimetric Notes on the measurement:
method).
• Certain photometers may require a blank (preparation as per
The method is analogous to EPA 375.4, APHA 4500-SO42- E, and
measurement sample, but with distilled water instead of sample).
ASTM D516-16.
• When using the 50-mm cell, perform the measurement against a
separately prepared blank (preparation as per measurement sample,
2. Measuring range and number of determinations but with distilled water instead of sample).
Configure the photometer for blank measurement.
Cell Measuring range Number of
mm mg/l SO42- determinations • For turbidimetric measurement the cells must be clean.
Wipe, if necessary, with a clean dry cloth.
50 0.50- 10.00
• The pH of the measurement solution must be within the range 1.0 - 2.0.
20 1.3 - 25.0 100
10 2.5 - 50.0 • The turbidity of the measurement solution remains stable for
only 2 min max. after the end of the reaction time stated above.
For programming data for selected photometers / spectrophotometers see
[Link]/photometry. 8. Analytical quality assurance
recommended before each measurement series
3. Applications To check the photometric measurement system (test reagents, measure-
Sample material: ment device, handling) and the mode of working, a dilute sulfate standard
Groundwater and surface water solution containing 5.00 mg/l SO42- can be used.
Drinking water and mineral water Sample-dependent interferences (matrix effects) can be deter-
Wastewater mined by means of standard addition.
Nutrient solutions for fertilization Additional notes see under [Link].
Soils after appropriate sample pretreatment For quality and batch certificates for Spectroquant® test kits see the
Water for concrete in the construction industry website, where you will find all data in production control, that are
This test is not suited for seawater. determined in accordance with ISO 8466-1 and DIN 38402 A51.
4. Influence of foreign substances 9. Notes
This was checked individually in solutions containing 5 and 0 mg/l SO42-. • Reclose the reagent bottles immediately after use.
The determination is not yet interfered with up to the concentrations of • Information on disposal can be obtained at
foreign substances given in the table. Cumulative effects were not checked; [Link].
such effects can, however, not be excluded.
Concentrations of foreign substances in mg/l or %
Ag+ 1 Mn2+ 1000 EDTA 50
Al3+ 1000 NH4+ 1000 Hydrazine 1000
Ca2+ 1000 Ni2+ 500 Anionic
Cd2+ 1000 NO2- 1000 surfactants 1) 0.5
CN- 1000 NO3- 50 Cationic
CO32- 1000 Pb2+ 2 surfactants 2) 250
Cr3+ 5 PO43- 100 Nonionic
Cr2O72- 2 S2- 5 surfactants 3) 250
Cu2+ 1000 SiO32- 1000 Na-acetate 10 %
F- 100 SO32- 5 NaCl 2%
Fe3+ 50 S2O32- 2
Hg2+ 500 Zn2+ 1000
Mg2+
1000
1)
tested with Na-dodecyl sulfate
2)
tested with N-cetyl-N,N,N-trimethylammonium bromide
3)
tested with Triton® X-100
5. Reagents and auxiliaries
Please note the warnings on the packaging materials!
The test reagents are stable up to the date stated on the pack when
stored closed at +15 to +25 °C.
Package contents:
1 bottle of reagent SO4-1
1 bottle of reagent SO4-2
1 AutoSelector
Other reagents and accessories:
MQuant® Universal indicator strips pH 0 - 14, Cat. No. 109535
Sodium hydroxide solution 1 mol/l Titripur®, Cat. No. 109137
Hydrochloric acid 1 mol/l Titripur®, Cat. No. 109057
Membrane filters 0.45 µm
Sulfate standard solution Certipur®, 1000 mg/l SO42-, Cat. No. 119813
Pipettes for pipetting volumes of 0.50 and 10 ml
Rectangular cells 10, 20, and 50 mm (2 of each), Cat. Nos. 114946,
114947, and 114944
6. Preparation
• Analyze immediately after sampling.
• The pH must be within the range 2 - 10. Merck KGaA, 64271 Darmstadt, Germany,
Tel. +49(0)6151 72-2440
Adjust, if necessary, with sodium hydroxide solution or hydrochloric [Link]/photometry
acid. EMD Millipore Corporation, 400 Summit Drive
Burlington MA 01803, USA, Tel. +1-978-715-4321
• Filter turbid samples through a 0.45 µm membrane filter. Sigma-Aldrich Canada Co. or Millipore (Canada) Ltd.
2149 Winston Park, Dr. Oakville, Ontario, L6H 6J8
Phone: +1 800-565-1400
