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Experiment-1 Calibration Practical

The document outlines a practical experiment for calibrating weights and volumetric glassware used in pharmaceutical analysis, including volumetric flasks, pipettes, and burettes. It details the procedure for each calibration, emphasizing the importance of accuracy and tolerance limits for each apparatus. The results indicated that all apparatus were successfully calibrated within acceptable tolerance limits.

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1K views3 pages

Experiment-1 Calibration Practical

The document outlines a practical experiment for calibrating weights and volumetric glassware used in pharmaceutical analysis, including volumetric flasks, pipettes, and burettes. It details the procedure for each calibration, emphasizing the importance of accuracy and tolerance limits for each apparatus. The results indicated that all apparatus were successfully calibrated within acceptable tolerance limits.

Uploaded by

lochabkhushi501
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Pharmaceutical Analysis Practical File

Experiment No. 1To calibrate weights and glasswares used in pharmaceutical analysis

Aim:

To calculate the weight and calibrate the volumetric apparatus like volumetric flask, pipette, and burette.

Apparatus Required:

Volumetric flask, pipette, burette, distilled water, digital balance, beakers, stoppered flask, tissue paper.

Theory:

The accuracy of volumetric analysis depends on the volume delivered or contained by the glassware. The actual volume

delivered is often slightly different from the nominal capacity mentioned on the apparatus. Hence, calibration is essential.

Principle:

Calibration is done by weighing the amount of water delivered or contained by the apparatus. The weight of water is

then converted to volume using the density of water.

Procedure:

1. Calibration of Volumetric Flask:

a. Take a clean and dried volumetric flask.

b. Weigh the empty flask and record its weight.

c. Fill the flask with distilled water up to the graduation mark (meniscus level).

d. Stopper the flask and reweigh.

e. Calculate the difference in weight - which is the weight of water.

f. Compare it with the tolerance limit.

Tolerance Limits:

- 10 ml 0.02 ml

- 25 ml 0.03 ml

- 50 ml 0.05 ml

- 100 ml 0.06 ml
Pharmaceutical Analysis Practical File

2. Calibration of Pipette:

a. Take a clean and dried pipette and weigh it.

b. Fill the pipette with distilled water till the mark (meniscus).

c. Transfer the water into a dried and weighed stoppered flask.

d. Stopper the flask and reweigh it.

e. Calculate the difference in weight = water delivered by pipette.

f. Compare it with standard tolerance.

Tolerance Limits (Transfer Pipette):

- 10 ml 0.02 ml

- 25 ml 0.03 ml

- 50 ml 0.05 ml

3. Calibration of Burette:

a. Take a clean and dried burette and weigh it.

b. Fill with distilled water and set the level at zero mark.

c. Let it stand for 10 minutes to remove air bubbles.

d. Open the stopper and let the water flow into a dried, clean, and weighed volumetric flask.

e. Stopper the flask and reweigh.

f. Calculate the volume delivered by difference in weight.

g. Check for tolerance limits.

Tolerance Limits (Burette):

- 10 ml 0.01 ml

- 25 ml 0.03 ml

- 50 ml 0.05 ml

4. Calibration of Fractional Weight:

a. Place the digital balance on a flat surface and zero it.

b. Place a reference weight (R) and record the reading as R.


Pharmaceutical Analysis Practical File

c. Replace R with another test weight (T) and record it as t.

d. Remove T and again place it to take t.

e. Again place R and take its reading as R.

Formula:

MT - R = [(t + t)/2 - (R + R)/2]

MT = MR + MT - R

Where:

MR = Measured Reference weight

MT = True value of T

= correction factor (usually 1 if not given)

Result:

All apparatus were successfully calibrated and found within acceptable tolerance limits.

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