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Inducing Accelerate

The document provides detailed insights into various aspects of plant breeding, including ideotype breeding, novel techniques for achieving homozygosity, and comparisons between traditional and modern breeding methods. It also discusses the floral structures of specific crops like wheat, barley, brinjal, and tomato, as well as hybridization techniques and the importance of broadening the genetic base in crops like chickpea. Additionally, it outlines breeding objectives for mustard and nutritional components of cowpea, emphasizing the significance of genetic diversity and modern breeding practices in enhancing crop resilience and yield.

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0% found this document useful (0 votes)
14 views15 pages

Inducing Accelerate

The document provides detailed insights into various aspects of plant breeding, including ideotype breeding, novel techniques for achieving homozygosity, and comparisons between traditional and modern breeding methods. It also discusses the floral structures of specific crops like wheat, barley, brinjal, and tomato, as well as hybridization techniques and the importance of broadening the genetic base in crops like chickpea. Additionally, it outlines breeding objectives for mustard and nutritional components of cowpea, emphasizing the significance of genetic diversity and modern breeding practices in enhancing crop resilience and yield.

Uploaded by

shankarin370
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

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1.​ Factor to be consider for Ideotype Breeding:
○​ Ideotype Breeding: The concept of developing an ideal plant type (ideotype)
with specific morphological and physiological traits that lead to maximum
yield or performance in a defined environment.
○​ Factors to Consider:
■​ Target Environment: Specific climatic conditions (temperature, rainfall),
soil type, and management practices (e.g., irrigated vs. rainfed, high
fertility vs. low fertility).
■​ Yield Components: Traits directly contributing to yield (e.g., number of
grains/seeds per panicle/pod, grain weight, number of effective tillers).
■​ Physiological Traits: Photosynthetic efficiency, water use efficiency,
nutrient uptake efficiency, partitioning of assimilates.
■​ Morphological Traits: Plant height, leaf angle, leaf area, lodging
resistance (strong stem), root architecture, maturity duration.
■​ Resistance/Tolerance: Resistance to major diseases and pests, tolerance
to abiotic stresses (drought, heat, salinity, cold).
■​ Quality Traits: Nutritional quality (protein, oil, vitamins), processing
quality (e.g., milling, baking).
■​ Harvest Index: The ratio of economic yield to total biological yield.
■​ Cropping System Compatibility: Suitability for intercropping, sequential
cropping, or specific planting densities.
■​ Economic Viability: Acceptance by farmers and markets.
2.​ Novel breeding techniques to bring homozygosity:
○​ Achieving homozygosity (genetically uniform individuals) is crucial for
developing stable varieties in self-pollinated crops and parental lines for
hybrids.
○​ Novel Techniques:
■​ Haploid and Doubled Haploid (DH) Breeding: This is the most rapid way
to achieve complete homozygosity. Haploid plants (containing a single set
of chromosomes) are produced from gametes (pollen or ovules) and then
chromosome number is doubled (e.g., using colchicine) to produce fertile,
completely homozygous doubled haploid lines in a single generation.
■​ Marker-Assisted Selection (MAS) for Homozygosity: While not directly
inducing homozygosity, MAS can significantly accelerate selection for
homozygous genotypes by using molecular markers to identify
homozygous loci at early generations (e.g., in F2 or F3) without extensive
progeny testing.
■​ Speed Breeding: A technique that accelerates the generation cycle (e.g.,
by extending light periods, optimizing temperature, using hydroponics) to
allow multiple generations per year, thus reaching homozygosity faster
through repeated selfing. While not directly inducing homozygosity, it
quickens the traditional selfing process.
3.​ Compare bt Traditional v/s modern breeding methods & justify your
answers:
○​ Traditional Breeding Methods:
■​ Reliance on Phenotypic Selection: Selection based on visible traits.
■​ Long Breeding Cycles: Takes many generations (e.g., 8-10 years) to
develop a new variety due to reliance on selfing and progeny testing.
■​ Limited Genetic Variation: Primarily uses existing variation within
cultivated gene pools.
■​ Labor Intensive: Requires extensive field evaluation and visual
assessment.
■​ Less Precise: Phenotype can be influenced by environment.
■​ Examples: Mass selection, pureline selection, pedigree method, bulk
method.
○​ Modern Breeding Methods:
■​ Integration of Molecular Tools: Uses DNA markers, genomics,
bioinformatics.
■​ Accelerated Breeding Cycles: Techniques like doubled haploidy and
speed breeding drastically reduce time.
■​ Broader Genetic Base: Facilitates gene transfer from wild relatives and
precise gene editing.
■​ High-Throughput and Automation: Utilizes phenomics platforms,
robotics, and advanced data analysis.
■​ High Precision: Marker-assisted selection and genomic selection allow
for selection based on genotype, less influenced by environment.
■​ Examples: Marker-assisted selection (MAS), Genomic selection (GS),
Doubled Haploidy, Gene Editing (CRISPR-Cas9), Speed Breeding, Genetic
Engineering (Transgenesis).
○​ Justification: Modern breeding methods are superior because they are
faster, more precise, can utilize a broader range of genetic diversity, and
are more efficient in developing improved varieties that address complex
challenges like climate change, new pest/disease threats, and increasing food
demand. They allow for the integration of multiple desirable traits more
effectively.
4.​ Compare floral structure of wheat & barley:
○​ Both wheat (Triticum aestivum) and barley (Hordeum vulgare) are
self-pollinated cereals belonging to the Poaceae (grass) family, sharing a
similar basic floral structure called a floret, which is part of a spike.
○​ Similarities:
■​ Both have florets enclosed by two bracts: lemma (outer) and palea
(inner).
■​ Each floret typically contains three stamens (anthers with pollen) and a
single pistil (ovary with a feathery stigma).
■​ Small, scale-like structures called lodicules swell to push open the lemma
and palea during anthesis (flowering).
■​ Florets are arranged on a central axis (rachis) forming a spike.
○​ Differences:
■​ Number of Florets per Spikelet:
■​ Wheat: Typically has 3-5 florets per spikelet, with only 1-3 often being
fertile.
■​ Barley: Has 3 florets per spikelet. In two-row barley, only the central
floret is fertile, while in six-row barley, all three florets are fertile.
■​ Awns:
■​ Wheat: Awns (bristle-like appendages) may or may not be present
(awned vs. awnless varieties).
■​ Barley: Awns are typically prominent and long on the lemma, giving
the barley spike a characteristic appearance.
■​ Adherence of Lemma/Palea to Grain:
■​ Wheat: Grains are usually free-threshing (lemma and palea separate
from the grain during threshing).
■​ Barley: Grains are often hulled (lemma and palea remain attached to
the grain after threshing, though hulless varieties exist).
5.​ Key differ in floral biology of brinjal & tomato:
○​ Both brinjal (Solanum melongena) and tomato (Solanum lycopersicum)
belong to the Solanaceae family and are predominantly self-pollinated, but
they have subtle differences in their floral biology impacting breeding.
○​ Brinjal (Eggplant):
■​ Flower Type: Hermaphroditic (perfect flowers, having both male and
female parts).
■​ Pollination: Predominantly self-pollinated. However, natural
cross-pollination can occur up to 10-20% (or even higher depending
on insect activity), mainly by bees and other insects due to the larger,
showier flowers and slightly exerted stigma in some types.
■​ Stigma Exertion: Can exhibit different flower types based on style length:
■​ Long-styled flowers: Stigma is exerted beyond the anthers, allowing
for potential cross-pollination. These are generally fertile.
■​ Medium-styled flowers: Stigma is level with or slightly below the
anthers.
■​ Short-styled flowers: Stigma is much shorter than the anthers,
usually sterile or less fertile.
■​ Pseudo-short styled flowers: Very short styles and often sterile.
■​ Anther Cone: Anthers are connivent (form a cone) around the style.
■​ Pollen Release: Pollen is often shed through apical pores (poricidal
dehiscence).
○​ Tomato:
■​ Flower Type: Hermaphroditic (perfect flowers).
■​ Pollination: Highly self-pollinated, with natural cross-pollination
typically very low (less than 5%). This is because the stigma is usually
enclosed within the anther cone (introrse stigma), making
self-pollination very efficient.
■​ Stigma Position: Generally introrse (stigma within the anther cone or at
the same level). Exserted stigma varieties exist but are less common.
■​ Anther Cone: Anthers are connivent, forming a tight cone around the
style.
■​ Pollen Release: Pollen is shed through apical pores, often requiring 'buzz
pollination' (vibration) by insects like bumblebees for efficient release,
though self-pollination can occur without it.
○​ Key Difference for Breeding: The higher natural cross-pollination rate in
brinjal due to more frequent stigma exertion means that isolation distances
for maintaining varietal purity are more critical for brinjal compared to tomato.
This also affects hybrid seed production strategies.
6.​ Process of fertilization & pollination in wheat:
○​ Pollination in Wheat:
■​ Wheat is a self-pollinated crop.
■​ When the wheat flower matures, the lodicules (small, fleshy structures at
the base of the ovary) swell, causing the lemma and palea to open.
■​ The anthers (which are pendulous) dehisce (split open) to release pollen
grains.
■​ The feathery stigmas are exposed.
■​ Most of the pollen falls directly onto the stigmas of the same flower
(autogamy).
■​ Some pollen may be carried by wind to other flowers on the same plant or
neighboring plants (limited anemophily/cross-pollination, usually <1-2%).
○​ Fertilization in Wheat (Double Fertilization):
■​ A pollen grain lands on the receptive stigma.
■​ The pollen grain germinates, forming a pollen tube that grows down
through the style, carrying two male gametes (sperm nuclei).
■​ The pollen tube enters the ovule through the micropyle.
■​ Inside the embryo sac (female gametophyte) of the ovule:
■​ First Fertilization: One male gamete fuses with the egg cell to form
the zygote (2n), which develops into the embryo (the future plant).
■​ Second Fertilization: The other male gamete fuses with the central
cell nucleus (containing two polar nuclei, forming a 3n primary
endosperm nucleus), to form the primary endosperm nucleus. This
develops into the endosperm, which is the primary food storage
tissue for the developing embryo and the bulk of the wheat grain.
■​ This dual fusion process is known as double fertilization, characteristic
of angiosperms.
■​ The ovule then develops into the seed (grain), and the ovary matures into
the fruit (caryopsis).
7.​ Hybridization techniques in wheat:
○​ While wheat is primarily self-pollinated, hybridization is fundamental for
creating new genetic combinations for selection.
○​ Steps for Artificial Hybridization:
1.​ Selection of Parents: Choose two parents with complementary desirable
traits.
2.​ Emasculation: This is the crucial step to prevent self-pollination in the
female parent. In wheat, it's typically done by hand:
■​ Clipping Method: Spikelets are selected, and the upper and lower
florets are removed. The anthers are then carefully removed from the
remaining florets with fine forceps before they dehisce. This is usually
done late in the evening or early morning.
3.​ Bagging of Emasculated Spikes: The emasculated spikes are covered
with butter paper bags to prevent contamination from foreign pollen.
4.​ Collection of Pollen: Pollen is collected from the male parent (donor)
when its anthers are dehiscing, typically by gently shaking the anthers into
a small bag.
5.​ Pollination: The collected pollen is dusted onto the feathery stigmas of
the emasculated female parent, usually within 24 hours of emasculation.
6.​ Re-bagging and Tagging: The pollinated spike is re-bagged and tagged
with details of the cross (parents, date).
7.​ Seed Development: The pollinated florets develop into hybrid seeds (F1).
These F1 seeds are then harvested and grown out for further generations.
8.​ Nutritional components of cowpea: (Already covered in the previous set of
answers, point 17)
○​ Protein: High (23-25%), essential for body functions.
○​ Dietary Fiber: Aids digestion, blood sugar regulation.
○​ Complex Carbohydrates: Sustained energy.
○​ Vitamins: Folate, thiamine, niacin (B vitamins).
○​ Minerals: Iron, zinc, phosphorus, magnesium, potassium.
○​ Antioxidants: Polyphenols.
9.​ U triangle: (Already covered in the previous set of answers, point 5 and 16)
○​ Describes the genetic relationships among Brassica species, showing how
three diploid genomes (A, B, C) combine to form three allotetraploid species.
10.​Breeding objectives in mustard:
○​ Mustard (referring to Brassica juncea, Indian Mustard, or Brassica napus,
Rapeseed/Canola)
○​ Key Objectives:
■​ High Seed Yield: Primary goal for economic returns.
■​ Improved Oil Content and Quality:
■​ Low erucic acid content (e.g., "Canola quality" with <2% erucic acid).
■​ Low glucosinolate content (for meal quality for animal feed).
■​ High oleic acid or desired fatty acid profile.
■​ Resistance to Biotic Stresses:
■​ Diseases: White rust (Albugo candida), Alternaria blight, downy
mildew, Sclerotinia stem rot.
■​ Pests: Aphids, painted bug.
■​ Resistance to Abiotic Stresses: Drought, heat, cold/frost tolerance,
salinity tolerance.
■​ Early Maturity: To fit into various cropping systems.
■​ Lodging Resistance: Stronger stems to prevent falling over.
■​ Shattering Resistance: Preventing seed loss before or during harvest.
■​ Wider Adaptability: To perform well across different regions and
environments.
■​ Improved Meal Quality: For use as animal feed after oil extraction.
11.​ Important of broadening of genetic base in chickpea:
○​ Genetic Base: Refers to the total genetic diversity available within a species
or breeding program.
○​ Importance of Broadening the Genetic Base in Chickpea:
■​ Increased Genetic Diversity: The cultivated chickpea (Cicer arietinum)
has a relatively narrow genetic base due to historical domestication
bottlenecks and subsequent breeding efforts focusing on limited
germplasm. Broadening it introduces new alleles.
■​ Source of Novel Traits: Wild relatives (Cicer reticulatum, C.
echinospermum, etc.) are a rich source of valuable genes for traits not
present in cultivated varieties, such as:
■​ Enhanced Disease Resistance: For evolving races of Fusarium wilt,
Ascochyta blight, Botrytis grey mould, and stunt virus.
■​ Improved Pest Resistance: Against pod borer, storage pests.
■​ Superior Abiotic Stress Tolerance: Genes for enhanced drought,
heat, cold, and salinity tolerance.
■​ Nutritional Quality: Potential for genes improving protein quality,
micronutrients.
■​ Yield Stability: New alleles can contribute to more stable yields
across diverse environments.
■​ Durable Resistance: Introducing multiple resistance genes from diverse
sources makes it harder for pathogens/pests to overcome resistance.
■​ Adaptation to Climate Change: Provides genetic raw material for
adapting to unpredictable and extreme weather events.
■​ Long-Term Breeding Progress: Prevents genetic erosion and ensures
continued progress in breeding by providing fresh genetic variation for
future selection cycles.
12.​Ideotype breeding: (Already covered in point 1)
○​ Developing an ideal plant type with specific morphological and physiological
characteristics for optimal performance in a given environment.
13.​Novel breeding techniques: (Already covered in point 2)
○​ Haploid/Doubled Haploid breeding, Marker-Assisted Selection for
homozygosity, Speed Breeding.
14.​Gene pyramiding & Gene editing:
○​ Gene Pyramiding (Stacking Genes):
■​ Definition: The process of combining multiple desirable genes (especially
resistance genes) from different sources into a single variety.
■​ Purpose: To achieve more durable and broad-spectrum resistance. For
example, combining 3-4 different disease resistance genes that each
confer resistance to different races of a pathogen makes it much harder
for the pathogen to overcome all resistances simultaneously.
■​ Methodology: Typically involves multiple rounds of crosses and
backcrosses, combined with marker-assisted selection (MAS) to track the
presence of each individual gene.
○​ Gene Editing (e.g., CRISPR-Cas9):
■​ Definition: A precise molecular tool that allows scientists to make
targeted changes to an organism's DNA at specific locations. This can
involve cutting DNA, deleting genes, inserting new genes, or making small
nucleotide changes.
■​ Mechanism (CRISPR-Cas9): Uses a guide RNA (gRNA) to direct a Cas9
enzyme (molecular scissors) to a specific DNA sequence, where it makes
a cut. The cell's natural repair mechanisms then fix the break, which can
lead to gene knockout (inactivating a gene), gene insertion, or base pair
changes.
■​ Application in Breeding:
■​ Knockout undesirable genes: Silencing genes responsible for
disease susceptibility, anti-nutritional factors, or undesirable traits.
■​ Introduce beneficial traits: Precisely inserting or modifying genes
for disease/pest resistance, abiotic stress tolerance, improved
nutritional quality (e.g., higher oleic acid in oils), or altered plant
architecture.
■​ Accelerate domestication: Mimicking natural mutations that
occurred during domestication.
■​ Advantage: Extremely precise, rapid, and does not typically involve
introducing foreign DNA from other species (unless specific gene
insertion is done), leading to products that may not be classified as GMOs
in some regulatory frameworks.
15.​Hybridization techniques in Rabi Solanaceous crops:
○​ Rabi Solanaceous Crops: Primarily referring to Brinjal (Eggplant) and
Chilli/Capsicum, which are often grown in the Rabi season. Tomato can also
be.
○​ Hybridization Technique (Manual):
1.​ Selection of Parents: Choose two genetically distinct parents with
complementary traits.
2.​ Emasculation (Female Parent): This is crucial to prevent self-pollination.
■​ Select healthy, unopened flower buds that are about to open (usually
the next day).
■​ Carefully remove the anthers using fine forceps before they dehisce.
This is typically done in the evening or early morning.
■​ In brinjal, consider the style length for successful emasculation
(long-styled flowers are preferred).
3.​ Bagging of Emasculated Flowers: Cover the emasculated flower with a
butter paper bag to protect it from foreign pollen.
4.​ Pollen Collection (Male Parent): Collect fresh pollen from the male
parent on the day of pollination. This can be done by gently tapping
dehisced anthers onto a watch glass or paper.
5.​ Pollination: Apply the collected pollen to the receptive stigma of the
emasculated female flower. This is best done in the morning when the
stigma is most receptive.
6.​ Re-bagging and Tagging: Re-bag the pollinated flower and attach a tag
with details of the cross (parents, date).
7.​ Hybrid Fruit/Seed Development: Allow the fruit to develop. Harvest
hybrid seeds from the matured fruit.
○​ Note on Self-Incompatibility: While these are generally self-compatible,
some wild relatives or specific lines might exhibit self-incompatibility, which
needs to be considered in parent selection.
16.​Sunflower breeding achievements: (Already covered in the previous set of
answers, point 21)
○​ Improved oil content and quality (high oleic/linoleic).
○​ Hybrid development for heterosis.
○​ Disease resistance (downy mildew, rust, Sclerotinia).
○​ Abiotic stress tolerance.
○​ Increased yield and yield stability.
17.​Maintenance breeding:
○​ Definition: Maintenance breeding refers to the ongoing efforts required to
maintain the genetic purity, trueness-to-type, and vigor of a released crop
variety. It ensures that the variety continues to perform as expected over time
and does not deteriorate due to genetic drift, outcrossing, or mechanical
mixture.
○​ Why it's Important:
■​ Preventing Genetic Contamination: To avoid admixture with other
varieties or wild relatives through accidental outcrossing, mechanical
mixing during planting/harvesting, or mutations.
■​ Maintaining Varietal Characteristics: Ensuring that the unique desirable
traits (yield, quality, disease resistance, maturity) of the variety remain
consistent across generations.
■​ Preserving Vigor and Adaptability: Preventing the decline in
performance that can occur if purity is lost.
○​ Methods Involved:
■​ Nucleus Seed Production: Starting with a small number of genetically
pure individual plants (breeder seed source) and carefully monitoring their
progeny.
■​ Rouging: Regularly removing off-type plants (plants not true to the
variety) from seed multiplication plots.
■​ Strict Isolation Distances: Maintaining physical separation between
different varieties to prevent cross-pollination.
■​ Careful Harvesting and Storage: Preventing mechanical mixing during
field operations, drying, and storage.
■​ Regular Monitoring and Evaluation: Periodically testing the variety for
its original characteristics.
■​ Seed Certification: Following strict guidelines to ensure quality and
purity at different stages of seed production (breeder, foundation,
certified seed).
18.​Breeding technology for drought resistance in Rabi crops:
○​ Rabi Crops: Wheat, Barley, Chickpea, Lentil, Mustard, Safflower.
○​ Challenges: Drought is a major abiotic stress limiting yield in rainfed Rabi
areas.
○​ Breeding Technologies/Approaches:
1.​ Phenotypic Selection:
■​ Direct Selection for Yield under Drought: Identifying lines that
perform well in drought-prone environments.
■​ Selection for Physiological Traits:
■​ Root System Architecture: Deeper and more extensive root
systems for better water uptake.
■​ Water Use Efficiency (WUE): Higher biomass production per unit
of water transpired (e.g., selection for higher carbon isotope
discrimination).
■​ Osmotic Adjustment: Ability to maintain turgor under water
stress.
■​ Early Vigor and Rapid Ground Cover: To reduce evaporative
losses.
■​ Leaf Traits: Smaller, thicker leaves, waxier cuticles, efficient
stomatal regulation.
■​ Stay-Green Trait: Retention of green leaves longer under
drought, which contributes to grain filling.
■​ Early Maturity/Drought Escape: Breeding for varieties that complete
their life cycle before severe drought sets in.
2.​ Conventional Breeding Methods:
■​ Hybridization: Crossing drought-tolerant landraces/wild relatives
with high-yielding susceptible varieties.
■​ Recurrent Selection: For improving complex, polygenic traits like
drought tolerance.
■​ Backcross Breeding: For transferring specific drought resistance
genes from donors.
3.​ Molecular Breeding (MAS/GS):
■​ Marker-Assisted Selection (MAS): Using DNA markers linked to
Quantitative Trait Loci (QTLs) for drought tolerance (e.g., for root
traits, WUE, osmotic adjustment).
■​ Genomic Selection (GS): Using genome-wide markers to predict the
breeding value of individuals for complex traits like drought tolerance,
even in early generations, accelerating selection.
4.​ High-Throughput Phenotyping (Phenomics): Using automated systems
(e.g., drones, sensors, imaging) to rapidly and accurately measure
drought-related traits in large populations, overcoming the phenotyping
bottleneck.
5.​ Genetic Engineering/Gene Editing:
■​ Introducing genes from other organisms or modifying existing genes
to enhance drought tolerance pathways (e.g., genes for osmotic
stress protection, antioxidant enzymes, or regulatory genes).
■​ While promising, commercial adoption faces regulatory hurdles.
6.​ Association Mapping and GWAS (Genome-Wide Association
Studies): To identify novel genes and QTLs associated with drought
tolerance in diverse germplasm.
19.​Breeding objectives in oats:
○​ Oats (Avena sativa) are primarily grown for grain (human consumption,
animal feed) and forage.
○​ Key Objectives:
■​ High Grain Yield: Primary objective for economic returns.
■​ Improved Grain Quality:
■​ High beta-glucan content (for health benefits in human consumption).
■​ High groat percentage (ratio of dehulled grain to whole grain).
■​ High protein content and balanced amino acid profile (especially for
feed).
■​ Low hull content.
■​ Disease Resistance:
■​ Crown rust (Puccinia coronata).
■​ Stem rust (Puccinia graminis).
■​ Smuts (loose and covered).
■​ Barley Yellow Dwarf Virus (BYDV).
■​ Lodging Resistance: Stronger stems to prevent falling over, especially in
high-fertility environments.
■​ Winter Hardiness/Cold Tolerance: For winter oat types.
■​ Drought Tolerance: For cultivation in drier regions.
■​ Shattering Resistance: To prevent grain loss before or during harvest.
■​ Early/Synchronous Maturity: To fit into cropping systems.
■​ Forage Yield and Quality: For forage oats, high biomass yield and good
nutritional quality (digestibility, protein).
■​ Wider Adaptability: To different environments and management systems.
20.​Sunflower breeding for oil content & biotic stress:
○​ Oil Content:
■​ Objective: Increase the percentage of oil in the seeds (currently 40-50%
in elite hybrids).
■​ Breeding Approaches:
■​ Heterosis (Hybrid Breeding): Exploiting hybrid vigor (CMS/Restorer
system) is the most effective way to boost oil content and yield.
■​ Selection: Continuous selection for higher oil percentage in breeding
populations.
■​ Gene Discovery: Identifying genes/QTLs associated with oil
biosynthesis and accumulation.
■​ Quality Traits: Breeding for specific fatty acid profiles like high oleic
acid (monounsaturated, stable oil) or high linoleic acid
(polyunsaturated). This involves targeting genes involved in fatty acid
desaturase pathways.
○​ Biotic Stress (Diseases & Pests):
■​ Objectives: Incorporate durable and broad-spectrum resistance to major
diseases and pests.
■​ Breeding Approaches:
■​ Gene Introduction: Using wild Helianthus species as a rich source of
resistance genes.
■​ Backcross Breeding: To transfer specific single or multiple resistance
genes (R-genes) into elite hybrid parental lines.
■​ Gene Pyramiding: Combining multiple R-genes for a single disease
(e.g., different R-genes for downy mildew) to provide more stable
resistance against evolving pathogen races.
■​ Molecular Breeding (MAS): Using markers linked to resistance genes
to select for resistant genotypes efficiently.
■​ Screening: Rigorous field and greenhouse screening under artificial
inoculation/infestation.
■​ Major Diseases: Downy mildew (Plasmopara halstedii), Rust (Puccinia
helianthi), Sclerotinia diseases (head rot, stem rot, wilt), Phomopsis
stem canker.
■​ Major Pests: Sunflower moth, sunflower stem weevil, cutworms,
aphids.
21.​Ideotype breeding for sustainable agriculture:
○​ Sustainable Agriculture: Practices that are environmentally sound,
economically viable, and socially just, ensuring long-term productivity without
degrading natural resources.
○​ Role of Ideotype Breeding:
■​ Resource Use Efficiency: Developing ideotypes that efficiently utilize
water, nutrients (N, P), and sunlight, reducing the need for external inputs
(fertilizers, irrigation), thus lowering environmental impact.
■​ Example: Ideotypes with deeper root systems for drought tolerance,
efficient nutrient uptake mechanisms.
■​ Reduced Chemical Input: Breeding for inherent resistance to diseases
and pests reduces reliance on pesticides, benefiting biodiversity, soil
health, and human health.
■​ Example: Ideotypes with pyramided resistance genes.
■​ Climate Change Adaptation: Designing ideotypes tolerant to extreme
temperatures (heat/cold), drought, or salinity, making agriculture more
resilient to changing climate patterns.
■​ Example: Ideotypes with improved photosynthetic efficiency under
stress, or altered phenology (earliness to escape terminal drought).
■​ Improved Soil Health: Varieties that fix nitrogen efficiently (legumes) or
have root systems that improve soil structure can contribute to soil fertility
and health.
■​ Yield Stability: Ideotypes that maintain stable yields across diverse and
often challenging environments, reducing risk for farmers and contributing
to food security.
■​ Biodiversity: While focusing on an 'ideal' type, ideotype breeding can
also consider incorporating diverse traits from wild relatives, contributing
to the genetic diversity within the cultivated species.
■​ Lower Carbon Footprint: More efficient resource use and reduced
chemical inputs can lead to a lower carbon footprint for crop production.
○​ Overall: Ideotype breeding moves beyond just maximizing yield under ideal
conditions to designing plants that are inherently more efficient, resilient, and
less dependent on external inputs, directly contributing to the goals of
sustainable agriculture.
22.​Ideotype breeding for higher yield, illustration achievement:
○​ Ideotype Breeding for Higher Yield:
■​ This involves conceptualizing and breeding for a plant architecture and
physiology that optimizes the capture and conversion of resources (light,
water, nutrients) into economic yield.
■​ Key Traits for Yield Ideotype:
■​ High Harvest Index: Maximize the proportion of biomass partitioned
to the economic part (grain, fruit).
■​ Optimal Plant Height: Dwarf or semi-dwarf stature to prevent
lodging (falling over) under high fertility and irrigation, allowing higher
fertilizer application and thus higher yield.
■​ Erect Leaf Angle: Allows better light penetration into the canopy,
optimizing photosynthesis in lower leaves.
■​ Efficient Photosynthesis: High photosynthetic rate and duration of
green leaves (stay-green trait).
■​ Robust Root System: Efficient nutrient and water uptake.
■​ Synchronous Flowering/Maturity: For uniform development and
easier harvest.
■​ High Sink Capacity: Ability of grains/fruits to accumulate assimilates.
○​ Illustrative Achievement (The Green Revolution - Wheat and Rice):
■​ The most prominent example is the development of semi-dwarf wheat
by Norman Borlaug and semi-dwarf rice by IRRI (International Rice
Research Institute).
■​ Achievement:
■​ Prior to Green Revolution: Tall wheat and rice varieties would lodge
(fall over) when high doses of nitrogen fertilizer were applied, limiting
yield potential.
■​ Ideotype Development: Breeders introduced genes for dwarfism
(e.g., Rht genes in wheat, sd1 gene in rice) that reduced plant height
significantly. This led to:
■​ Lodging Resistance: Plants could withstand higher fertilizer
applications without falling.
■​ Increased Harvest Index: More photosynthates were partitioned
to grain rather than stem elongation.
■​ Higher Yield Potential: Allowed for significant yield increases,
especially with improved management practices (fertilizer,
irrigation).
■​ Impact: This "high-yielding variety" (HYV) ideotype was central to the
Green Revolution, preventing widespread famine in many parts of the
world, particularly Asia, by dramatically increasing cereal production.
It's a classic example of how modifying plant architecture based on an
ideotype concept can lead to revolutionary yield gains.
23.​Sunflower hybrid varieties – mention one:
○​ Sunflower breeding extensively uses hybrid varieties for higher yield and other
desirable traits due to heterosis.
○​ Example Hybrid Variety:
■​ Pacom-36 (a widely known hybrid in India)
■​ Hysun 33 (another popular one)
■​ Pioneer 64A15 (a globally recognized hybrid from Pioneer/Corteva)
■​ (Note: Specific popular hybrids vary by region and seed company.)
24.​Safflower abiotic resistance:
○​ Safflower (Carthamus tinctorius) is naturally quite hardy, but breeding further
enhances its tolerance to abiotic stresses.
○​ Key Abiotic Stresses and Resistance/Tolerance:
■​ Drought Tolerance: Safflower is well-adapted to arid and semi-arid
conditions due to its deep root system, which allows it to extract water
from deeper soil profiles. Breeding aims to enhance this by selecting for
efficient water use, osmotic adjustment, and stress escape mechanisms.
■​ Salinity Tolerance: Relatively more tolerant to saline conditions
compared to many other crops, especially at later growth stages.
Breeding focuses on improving germination and seedling vigor under
saline conditions and overall yield stability.
■​ Heat Tolerance: Can withstand high temperatures, especially during
flowering and seed filling. Breeding focuses on maintaining yield and oil
quality under heat stress.
■​ Cold/Frost Tolerance: Some winter safflower varieties are developed to
withstand colder temperatures and frost during the vegetative stage.
■​ Nutrient Stress Tolerance: Can grow on marginal soils with limited
nutrient availability. Breeding aims to improve nutrient use efficiency,
especially for phosphorus.

I hope these answers are comprehensive and clear for your study!

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