Review Ahmed R.
compounds with similar adsorption properties set by organizations like the FDA and EMA. It is
and may require frequent replacement of saturated instrumental in the validation and verification of
columns. Extended analysis times for complex analytical methods, and in generating data required
samples can be a disadvantage when fast results are for regulatory submissions.
needed.
Forensic analysis: In forensic pharmaceutical
Overall, the article presents HPLC as a highly analysis, HPLC helps in the identification and
reliable and powerful analytical technique with quantification of drugs and their metabolites in
numerous applications. It effectively demonstrates samples related to criminal investigations or drug
HPLC's significancein modern analytical chemistry abuse cases.
by providing critical insights and supporting
Current use
scientific and industrial advancements. However,
the discussion also highlights areas for Currently, HPLC is integral to pharmaceutical
improvement, such as reducing costs, enhancing manufacturing processes, ensuring that products
speed, and minimizing contamination risks. By are consistent and meet the high standards required
comparing HPLC with other methods, the article by the industry. Advances in HPLC technology,
provides a nuanced perspective on the technique’s such as the development of faster and more efficient
unique advantages and areas where alternative columns and detectors, have improved its resolution
methods might be more appropriate. This balanced and sensitivity. These advancements allow for the
approach helps readers understand the best analysis of complex mixtures and lower detection
contexts for utilizing HPLC effectively. limits, enhancing the ability to detect minute
quantities of impurities.
Uses
High-Performance Liquid Chromatography
(HPLC): High-Performance Liquid
Future Developments
Chromatography (HPLC) is a critical analytical The future of HPLC in the pharmaceutical
technique across variousl sectors. Here’s an overview sector looks promising with several anticipated
of its uses, current applications, and potential future advancements:
developments.
Integration with other techniques: Combining
Uses of HPLC in pharmaceutical sectors HPLC with techniques like mass spectrometry (LC-
Drug development and quality control: HPLC MS) or nuclear magnetic resonance (LC-NMR) will
is extensively used in drug development to ensure continue to enhance its analytical capabilities. These
the purity and composition of new pharmaceutical integrations can provide more detailed information
compounds. During the development phase, it helps about molecular structures and interactions.
in characterizing drug substances and excipients, Miniaturization and automation: Advances in
and during quality control, it is essential for verifying miniaturization and automation are likely to make
that the final product meets required specifications. HPLC more efficient and cost-effective. Micro-
It detects impurities and degradation products, HPLC systems and automated sample handling
ensuring the safety and efficacy of the drugs. can increase throughput and reduce manual labor,
Pharmacokinetics and pharmacodynamics: In the making the technique more accessible for routine
study of pharmacokinetics (how the body affects a analyses.
drug) and pharmacodynamics (how the drug affects Advanced columns and materials: Development
the body), HPLC is used to measure drug levels in of new stationary phases and column materials will
biological samples, such as blood and urine. This improve separation efficiency and speed. Innovations
helps in understanding the absorption, distribution, such as monolithic columns and superficially porous
metabolism, and excretion of drugs, leading to better particles promise to enhance performance and
dosing regimens and therapeutic outcomes. reduce analysis times.
Clinical trials: HPLC is employed to monitor the Increased sensitivity and selectivity: Future HPLC
stability of drugs during clinical trials. It ensures systems will likely offer even greater sensitivity
that the drugs administered to participants are of the and selectivity, allowing for the detection of lower
correct concentration and free from contaminants, concentrations of analytes and a wider range of
thus maintaining the integrity of clinical trial data. compounds, including complex biomolecules and
Regulatory compliance: Pharmaceutical companies nanoparticles.
use HPLC to comply with regulatory standards Green chemistry: There is a growing emphasis on
533 Clin. Invest. (Lond.) (2024) 14(9)
