THE WEST BENGAL UNIVERSITY OF HEALTH SCIENCES

Bachelor of Medical Laboratory Technology

Training report on Blood Bank submitted

to The West Bengal University of Health
Sciencesoffor
Bachelor the Partial
Medical Fulfillment
Laboratory of the
Technology
Degree of Bachelor in Medical laboratory
Training at blood bank andTechnology
submission of report and discussion

Submitted by
Submitted by
Name -
Name – PURABI
Registration No: SAHOO
of
RegistrationInternship
No: 202204360 of 2022-23
Hospital and Address:
Internship Hospital and Address:
DEBRA SUPER SPECIALTY HOSPITAL.
DEBRA SUPER SPECIALITY HOSPITAL
Debra,
Debra,Paschim
PaschimMedinipur,
Medinipur,Pin
Pin-– 721124
721124

MIDNAPORE CITY CITY

MIDNAPORE COLLEGE
Dept. of Paramedical & Allied Health Sciences
COLLEGE
Vill- Kuturiya, P.O.- Bhadutala
Dept. of Paramedical & Allied Health
Pin-72112
SciencesKuturiya, P.O. Bhadutala, Pin-

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 Preface
Training for the one months in the Govt. / Govt. recognized hospital is an integral part of the
BACHELOR OF MEDICAL LABORATORY TECHNOLOGY (BMLT) program and is designed to
provide BMLT interns with an opportunity to integrate and apply previously acquired knowledge and
technical skills in actual clinical settings.

This training report is prepared with the intention to provide orientation to interns about various tasks
that have been performed and/or observed in different laboratories during one-month training at the
Govt. / Govt. recognized hospital. The main goal of the training is to acquire the necessary practical
skills in performing various laboratory tests in different laboratories in hospitals that will contribute
directly to efficient laboratory diagnosis and improve health care services.

The main contents of this report are the tasks that interns are either performed or observed during the
training. The interns prepared this brief report after completion of the one months training and
submitted it to the College/University for the evaluation to complete their study to obtain their degree
in BACHELOR OF MEDICAL LABORATORY TECHNOLOGY (BMLT)

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 Acknowledgment

I would first like to acknowledge Dr. Pradip Ghosh, Hon’ble Founder Director, Midnapore City College,
Paschim Medinipur for providing me the opportunity to study in this college and complete my Training in
Govt. Hospitals. I am gratefully indebted to him for his very valuable advice about our duty and responsibility
as a Lab technologist during the Training period and in future careers.

I would like to express my sincere thanks to the Principal, MCC for his kind suggestion, cooperation, and help
during our time at MCC as well as during the internship.

I would like to express my deepest sense of gratitude to the for his valuable advice and unending guidance to
complete my internship.

I would like to express my sincere thanks to Blood Centre, Ghatal Super Speciality Hospital for their valuable
advice, guidance, helping us to learn the modern techniques & applications in Biomedical aspects and co-
operations.

I would like to convey my sincere thanks to the technical staff of the Ghatal Super Speciality Hospital for their
kind co-operation & help throughout the training period.

I also convey my sincere thanks and gratitude to our Honourable Director Dr. Pradip Ghosh, (M.Tech, Ph.D.),
Principal Dr. Sudipta Chakrabarti, M.Sc., Ph.D., Vice Principal Dr. Kuntal Ghosh, M.Sc., Ph.D., Dr. Santanu
Kar Mahapatra(HOD),MSc, Ph.D. , Mr. Sourya Sekhar Mitra (Double M.sc), Mr. Sohel Rana (M.Sc., Ph.D.),
Dr. Monalisha karmakar (M.Sc.) and Ms.Dept. of Paramedical & Allied Health Sciences, MCC & all the
honorary guest teachers for their inspiration & advice throughout the courses as well as for the training &
report preparation. Without their passionate participation and input, the validation training could not have been
completed.

Finally, I must express my very profound gratitude to my parents and family members for providing me with
unfailing support and continuous encouragement throughout my years of study and during the training period,
and this report preparation. This accomplishment would not have been possible without them.

Thank you

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 Contents
1. Vision, Mission, Goals, and Objectives of the Internship

2. Training Specifics, Intern Information, Proposed Training Schedule

3. General Laboratory Safety Procedures and Rules

4. Training on Blood Bank

5. Intern Feedback/Comments on Internship

6. The output of the training& Conclusion

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 1. Vision, Mission, Goals, and Objectives of the Internship

1.1. Vision:
The BACHELOR OF MEDICAL LABORATORY TECHNOLOGY (BMLT) program is to be one of the
leading programs at the national and regional levels recognizing our graduates for their professional
competence, leadership quality, and competitive research in the field of MEDICAL LABORATORY
TECHNOLOGY.

1.2. Mission:
To provide innovative curricula responsive to the needs of the profession that result in medical laboratory
technicians being able to join immediately the blood bank field with the theoretical knowledge and the
technical skills necessary to provide blood bank services. Moreover, we will be prepared to be able to adapt to
the future changes in the health care system and clinical laboratory science.

1.3. Goals:
To prepare competent laboratory technicians who have acquired the necessary knowledge, skills, training, and
proficiency in the blood banking system.
1.4. Objectives:
Upon completion of the BACHELOR OF MEDICAL LABORATORY TECHNOLOGY program our
graduates are expected to:
a. Have in-depth knowledge of the relationships between laboratory data and transfusion processes, and how
laboratory data related to health and disease.
b. Have the talent to perform cross matching, blood grouping, cell separation etc in different clinical
laboratories in hospitals.
e. Have the ability to work independently and as a team member to perform critical thinking and problem-
solving skills in different diagnostic laboratory domains.
f. Have the capability to demonstrate an attitude of professionalism when working with colleagues with all
other health professional staff working in the diagnostic laboratory.

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 2. TRAINING SPECIFICS
2.1 Introduction:
The training is an integral part of the program for Bachelor of Medical Laboratory Technology and is designed
to provide interns with an opportunity to integrate and apply previously acquired knowledge and technical
skills in actual clinical settings. Under the guidance of experienced Medical Laboratory Professionals and other
qualified laboratory personnel and health professionals, interns learn more about diagnostic test procedures,
quality control methods and programs, and instrumentation in the clinical laboratory. They also gain an
understanding of the roles and functions of the Medical Laboratory technologist.

The training provides applied learning experiences during which the intern should:
a. Acquire blood banking laboratory skills
b. Perform quality control procedures
c. Learn and adapt new procedures
d. Operate and maintain various laboratory machines and instruments related to the blood transfusion.
e. Report accurate and precise results to supervisors.
f. Understand the responsibilities, roles, and functions of the Medical Laboratory technologist.

The training program is conducted in the laboratories of the Govt./ Govt. recognized hospital, where interns
learn by participating in the workload of a supervising technologist/specialist/consultant. The emphasis in each
training discipline is given on: a) organization of work, b) use of automated instrumentation, c) the relation of
laboratory results to patient diagnosis, and d) the establishment and use of programs for quality control and
preventive maintenance of laboratory instruments.
2.2 Training duration:
The training period for the training is 15 days.

2.3 Intern Information

1. Name of the intern - Purabi Sahoo
2. College Address - Kuturiya, Bhadutala, Midnapore, Paschim Medinipur, Pin- 721129, West
Bengal, India
3. Permanent Address - Vill-Kaniora, P.O-Ranisai, P.S-Ramnagar, Purbo Medinipur, Pin- 721146,
West Bengal, India
4. College ID card No - 04783/22-23

5. Registration no with Year - 202204360

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6. Roll no -
7. Mobile: - 8101191707

8. E-mail: - [email protected]

9. Hospital name and Address where training done – Debra Super Speciality Hospital.

2.4 PROPOSED TRAINING SCHEDULE

Blood Centre Dept. of Debra Super Speciality Hospital.

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 3. GENERAL LABORATORY SAFETY PROCEDURES AND RULES

3.1 Laboratory safety

We, the trainers, read and understand the information regarding laboratory safety and emergency procedures
before the first laboratory session. We adhere to written and verbal safety instructions throughout the training
period. Safety information was provided by the hospital safety officer before the beginning of the internship,
following general safety guidelines tha t help us to work in a safe environment during the training period.
Discipline-specific safety precautions are provided by the specific sections.
3.1.1 General laboratory safety
a. Always wear a laboratory coat or apron while working. After work, leave the lab coat in an assigned cabinet
or area.
b. Must wear personal protective equipment (gown, gloves, masks, face shield or glasses, etc.) when working
with hazardous or toxic materials and change when contaminated.
c. Shoes should be fluid impermeable material and cover the entire foot.
d. The application of cosmetics within the laboratory is strictly prohibited.
e. Contact lenses should not be worn while working in the laboratory.
f. Always cover any cut, insect bite, or open wound with a water-proof adhesive dressing.
g. Gloves should be removed (unless stated to wear) before handling telephones, computer keyboards,
laboratory equipment, doorknobs, etc
h. Eating, drinking, smoking, and chewing gum are prohibited in the laboratory.
i. Storage of food or drink is not allowed in laboratory refrigerators.
j. Mouth pipetting must not be done.
k. Laboratory working surfaces shall be decontaminated with a disinfecting solution after the spill of blood or
body fluid.
l. Needles should not be recapped or removed from a disposable syringe.
m. Discard used syringes, needles, and other sharps (glass slides, glass pipettes, knives, etc.) in specified
containers.
n. If equipment shows any problem while being used, report immediately to your supervisor. Never try to fix
the problem yourself.
o. Follow the standard safety precautions when using a centrifuge.
p. Hands should be washed with soap and water after handling hazardous a n d infectious material
q. Biological safety cabinets (Class I or II) should be used to avoid aerosolization or droplets.
r. Equipment contaminated with blood or other body fluids should be decontaminated and cleaned before use.

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s. All waste and contaminated materials (clinical specimens, bacterial cultures) should be disposed of in
appropriate containers.
t. Inform your supervisor about any accidents, spills, or potential hazards.
3.1.2 Chemical safety
a. Know the color coding and numerical rating of chemicals or materials for health hazards, fire hazard,
reactivity hazard, and specific hazards (reactivity with water) (NFPA label).
b. Use volatile and flammable compounds only in a fume hood.
c. Never return unused chemicals to their original container.
d. Dispose of chemical waste in proper containers according to manufacturer’s instructions.
3.1.3 Emergency response
a. Read safety and fire alarm posters and follow the instructions during an emergency.
b. Know the location of fire extinguishers, fire exits, first aid kits, and eyewash solutions in your lab and know
how to use them.
c. Know the building evacuation procedure in an emergency.

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 Training on Blood Bank

4.1 Blood Bank

Goal: Students need to acquire practical skills in proper phlebotomy techniques and blood storage and acquire
practical skills in standard blood bank techniques during the training period.
Objectives:
i. To disinfect the blood collection site with appropriate disinfectant.
ii. To know how to apply a tourniquet and for a desirable time.
iii. To detect the preferred venous access sites.
iv. To insert the needle properly for blood withdrawal.
v. To take care of the patient to avoid complications during and after the blood collection process.
vi. To categorize specimens according to their turnaround time.
vii. To develop technical accuracy and self-confidence by experiencing routine functions of the Blood Bank.
viii. To recognize and resolve discrepancies in blood grouping.
ix. To exhibit knowledge of standard techniques used for ABO and Rh typing, compatibility testing,
antibody identification, antigen typing, and preparation of blood components.
x. To acquaint with the procedures of donor selection and issuing of blood and blood products for
transfusion.
Tasks:

SL No. Ask Observed/Performed Specific Learning

1 Blood collection How to collect blood in Blood Collection
blood bank
2 Laboratory Blood Grouping Execution of whole blood
3 Instruments handled Centrifuge, Agitator How to perform those
4 Analysis of result Crossmatching Granted blood for
recipient
5 Blood storage Storage of whole blood in 2 How to Store blood
to 6oc
6 Documentation Report Study requisition and match Manage logbook and
preparation book also verify and update update those
stored component

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Methodology
Blood bank & blood transfusion unit:
a. In-house blood collection
b. Urgent laboratory
1. Blood grouping & Rh factor
2. Compatibility testing or cross-matching
3. Direct & indirect Coombs test
4. Du test

Clinical significance of blood transfusion:

Blood transfusion today is a major medical service that is rendered to patients needing replacement of whole
blood or blood components. Since the beginning of the Second World War, transfusion of blood & blood
products has become accepted as a routine & relatively safe procedure in the management of patients. The
blood bank tries to select a donor’s blood that will be compatible with the recipient’s blood. The recipient’s
plasma should not have any reactive antibody towards the red cell or donor which may lead to
hemagglutination or haemolysis of the donor’s red cells.
ABO blood groups:
During a blood transfusion, an identical ABO blood group of the donor is ideal. Persons belonging to blood
group “O” are considered to be “universal donors”, i.e. their blood can be given to individuals of any other
blood group. The red cells do not carry either A or B antigen & hence they do not react with their corresponding
antibodies. On the other hand, those with blood group AB are “universal recipients”. They will accept blood
from any of the blood groups-A, B, AB, or O. This is because they neither have anti-A nor anti-B. Transfusion
of specific component blood, as needed by the patient, is preferred over transfusion of whole blood. Whole
blood is needed in case of large- scale bloodless. The whole blood arrangements the recipient’s total blood
volume which may lead to congestive heart failure. Anaemic patients with adequate blood volume but who are
deficient in the red blood cell population require packed red cells. Transfusion of plasma, plasma components
& platelet concentrates is the therapeutic measure recommended for bleeding patients.

IN-HOUSE BLOOD COLLECTION

Blood collection procedure: Preparation:
Before starting to draw blood, sauce that the following materials are available & within reachable distance
properly labelled blood collection bottle with donor’s identification member; bleeding set & airway attached;
pilot tubes; tourniquet/pacer cuff; forceps; stripper; adhesive tape; rubber bond; savlon; alcohol; iodine swab;
local anaesthetic & syringe & needle for injection.

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Procedure:
1. The donor is advised to lie on the bleeding table & made sure he/she is relaxed & comfortable. The bottle
is placed approximately 30 – 40 below the level of the table.
2. The tourniquet/pacer cuff is applied to the upper arm & selects a prominent antecubital vein.
3. The tourniquet is released & disinfected venipuncture site using savlon, iodine & alcohol swab in that order.
The skin is allowed to dry before inserting the needle.
4. The tourniquet is re-applied & injected with local anaesthetic subcutaneously at the site of venipuncture
using an aseptic technique.
5. The venipuncture is performed with a 15 or 16- gauge needle. The bottle or bag is gently agitated to mix the
blood with anticoagulant. For this purpose, agitating machine may be used.
6. When the required amount of blood has been collected, a tube is clamped; the tourniquet is released &
removed the object is from the donor’s hand.
7. When the required amount of blood is collected before the needle has been removed from the veins, the
needle is drawn from the bottle & put a t 5 – 10 ml. Sample into 2 sterile, dry pilot test tubes, each labeled with
the same number as that of the main blood collection bottle.
8. An alcohol swab is placed over the needle & then removed. Pressure is applied over the puncture site with
an alcohol swab. The donor is asked to press swales firmly over the area for 3 – 5 minutes & preferably with
the arm held straight up in an extended position.
9. The airway needle from the blood collecting bottles is promptly removed & replaced with the cays after
applying a 70% alcohol swab in open areas.
10. Finally, tally the members on the bottle with the donor slip, and record the book.
11. A rubber band is put around the pilot tubes & collecting bottles.
12. Did not let the donor’s stands up immediately after blood donation. Make sure that any bleeding from the
venipuncture had stopped with a Band-Aid wound is covered.
13. The bottle is stored at 2 – 4⁰C.

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BLOOD BANK LABORATORY
DETERMINATION OF BLOOD GROUP BY CROSS-MATCH
Principle:
The house of blood transfusion has become steadily more frequent nowadays. To detect or to decide whether
the donor’s blood matches the recipient’s blood, the blood group test is done.
Requirements:
1. Glass slide
2. Pasteur pipette
3. Applicator sticks
Reagents:
1. Anti-A sera
2. Anti-B sera
3. Anti-D sera
4. Normal saline

Procedure:
1. A suspension of RBCs in normal saline is prepared.
2. On 1 half of the glass slide is placed 1 drop of Anti-A blood grouping sera.
3. On the other half of the glass slide placed 1 drop of Anti-B blood grouping sera & Anti- D sera.
4. Using a Pasteur pipette added 1 drop of the cell suspension to each half slide.
5. With separator applicator sticks mixed each cell's sera mixture well.
6. Tilt the slide back & forth & observed for agglutination.

ANTI-A ANTI-B ANTI-D PROBABLE BLOOD GROUP

+ - + A(+)

- + + B(+)

- - + O(+)

+ + + AB(+)

- - - O(-)

Agglutination: + No agglutination: -

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Significance: The use of blood group is: -
1. To ensure compatible blood transfusion,
2. To eliminate the hemolytic disease of the newborns due to Rh incompatibility,
3. To detect susceptibility to various diseases.

DIRECT & INDIRECT METHODS MUST GIVE COMPATIBILITY TESTING OR CROSS-

MATCHING
Before the recipient receives a blood transfusion, a compatibility test must be run within the laboratory with
the donor’s red cells & the recipient’s serum. This is called major cross- matching. The primary purpose of a
major crossmatch is to find out any incompatibility of the donor’s cells with the patient’s serum in order to
avoid transfusion reactions. The minor crossmatch is rarely requested when the compatibility of the recipient’s
red cells is tested against the donor’s serum.
A compatibility test or cross- matching is performed subsequent to the ABO grouping & Rh typing of the
recipient’s & donor’s blood. It is the final criterion as to the suitability of particular donor blood for a particular
recipient.
The recipient’s blood is obtained fresh while the donor’s blood is obtained from the pilot tube. ACD anti
coagulated donor’s blood should not be more than 21 days & constantly be stored at 4⁰C.
Principle:
The serum of the recipient is tested against the red cells of the donor under different conditions in order to
establish their compatibility or non-agglutination. Agglutination in any of the conditions indicates the presence
of incompatible antibodies inpatient, natural or immune.
The three phases of compatibility testing are listed below & illustrated in saline phases, where the immunologic
reaction between red cells suspended in a saline medium & the antibody occurs at room temperature.
1. Thermophase with protein: Where the red cells are suspended in the antibody (serum) with 22% albumin
(protein) & incubated for 30 minutes at 37⁰C.
2. Antihuman globulin (AHG) phase: Where the incubated cells are washed (to remove free globulin) & reacted
with antihuman globulin serum (Coombs reagent or antihuman globulin).

CROSS-MATCHING BY SLIDE METHOD

The above slide method is commonly done in the blood bank for quick cross-matching.

Requirements:
1. Glass slide, marker

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2. Disposable plastic sticks
3. Microscope
4. 4% red cell suspension of donor’s & recipient’s
5. Donor’s & recipient’s serum
Procedure:
1. Take a slide & draw a line centrally to divide into 2 parts.
2. Marked 1 part „P‟ for major cross-matching & part „D‟ for minor cross-matching.
3. On the „P‟ slide add 1 drop of patient’s serum & 1 drop of donor’s 4% cell suspension.
4. On the part of „D‟ add 1 drop of donor’s serum & 1 drop of patient’s cell suspension.
5. Mix the content of each slide by gently rotating the slide.
6. Incubate at room temperature (21 -25⁰C) for 10 minutes.
7. Examine both macroscopically & microscopically for agglutination.
Result:
There is no agglutination on both sides of the side that indicates the donor’s blood is compatible with the
recipient’s blood.
COOMBS TEST
Antihuman globulin technique is very useful in recognizing weak immunologic reactions. It is widely used in
the identification of Du compatibility testing, antibody screening & identification of sensitized red cells.
DIRECT COOMBS TEST
This recognizes sensitized red cells when the sensitizing occurs within the body, i.e, in hemolytic disease of
the newborn (HDN) & autoimmune haemolytic anaemia. This test is performed to detect anti-D antibodies or
other antibodies attached to the red cell surface within the bloodstream.
Principle:
Antihuman globulin or Coombs test detects sensitized re cells where the red cell gets coated with IgG
antibody or globulin but do not agglutinate when sensitized red cells come in contact with anti-human
globulin reagent they agglutinate.
Reagents:
1.Anti-human globulin or Coombs reagent
2.Pre-sensitized red cells or Coombs sensitive cells
3. Saline (0.85%)
Specimen:
The collected blood is preferred over whole blood (citrate) is required for a direct Coombs test. In case of an
indirect Coombs test for antibody screening serum specimen will be needed.

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Procedure:
1. Prepare 4% cell suspension in isotonic saline of the red blood cell to be tested.
2. With a clean pasture pipette add 1 drop of the prepared red cell suspension to a small test tube.
3. Wash 3 times with normal saline to remove all traces of serum or free globulin.
4. Decant completely after the last washing.
5. Add 2 drops of AHG serum to the sedimented cells.
6. Mix well & centrifuge for 1 minute at 1500 r.p.m.
7. Resuspend the cells by gentle agitation & examine macroscopically for agglutination.
8. Examine for agglutination by holding the tube against a lighted background & tapping the bottom of the
tube.

Observation:
If agglutination occurs - Positive Coombs test
If no agglutination - Negative Coombs test
INDIRECT COOMBS TEST
Principle:
Here the sensitization of red cells is done in the laboratory by incubating the red cells with the corresponding
antibody at 37⁰C for 30 minutes. The test is applied in detecting the presence of unexpected antibodies in the
serum which will react with the corresponding antigen on the red cells.
Specimen:
In t h e case of t h e indirect Coombs test for antibody screening, is needed it need not be a fasting sample.
Procedure:
1. Label their test tubes as T (test serum), PC (positive control), or NC (negative control).
2. In the T marked tube add 1 drop of test serum.
3. In the PC marked tube add 1 drop of Anti-D serum.
4. In the NC marked tube add 1 drop of saline or bovine albumin (22%).
5. In each test tube add 1 drop of 4% saline suspension of the pooled Rh +ve („O‟ group) cells or Coombs
sensitive cells.
6. Incubate all the three tubes at 37⁰C for 30 minutes.
7. Wash the cells 3 – 4 times with normal saline to remove excess serum or free globulin.
8. Add 2 drops of Coombs serum (AHG serum) to each tube & mix well.

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9. Keep for 5 minutes & centrifuge for 1 minute, at 1500 r.p.m.
10. Re-suspend the cells & examine macroscopically or microscopically for haemagglutination.

Observation:
If agglutination occurs - Positive Coombs test
If no agglutination - Negative Coombs test

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 INSTRUMENTAL DEMONSTRATION
Haematology Analyzer
With its simplified operation, the XP-300 is an ideal haematology analyzer for a clinic satellite laboratory. It
provides a CBC with 8 reportable parameters and 3-part WBC Differential, which includes an Absolute
Neutrophil Count (ANC). The results include histograms for WBC, RBC and PLT. The system provides a high
level of accuracy through the use of automatic floating discriminators. Built on reliable Sysmex technology, it
features a simple start-up menu and single button selection for sampling and daily maintenance with a compact,
space-saving design.
Reliability, Accuracy and Simplicity
a. Same direct current detection method as Sysmex high-end systems to provide accurate, comparable
results
b. Minimal training required
c. Simple menus
d. Push button technology
e. Non-toxic, biodegradable reagents
f. Reliable hardware and results
g. Network capability via your LIS

Fig: Haematology Analyzer

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 Microplate Washer

A wide variety of experimental assays require a series of washes. Microplate washers are laboratory
instruments designed to control the procedure of washing experimental samples arranged in plate-based
formats. Users load a plate and select a program; microplate washers then dispense, soak and aspirate liquids
from the plate in seconds. Compared to manual alternatives, microplate washers tremendously improve the
speed and accuracy of many different washing procedures, and are particularly useful for Enzyme-Linked
Immunosorbent Assays (ELISAs). Microplate washers are also employed to wash cell cultures, protein arrays,
Western blots and beads as well as applied in DNA purification protocols.

Fig: Microplate Washer

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 Portable Tube Sealer

The blood bag tube sealer is a compact equipment to seal the blood bags, infusion bags, urine bags by high
frequency sealing system. It's widely used in blood-bank, hospital, biological industry, etc. Features: * No
haemolysis. * With suction feet to avoid moving, can be used in blood collecting vehicle.

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 Work Report
DEBRA SUPER SPECIALITY HOSPITAL

Blood Banking

Blood Grouping:
Sl. No Blood Unit No: Age Sex Blood Group
1 MMC-25-410-1 29 M B (+)
2 MMC-25-410-2 20 M B (+)
3 MMC-25-410-3 35 F O (+)
4 MMC-25-410-4 45 M B (+)
5 MMC-25-410-5 32 F O (+)
6 MMC-25-410-6 48 M A (+)
7 MMC-25-410-7 37 M B (+)
8 MMC-25-410-8 32 M A (+)
9 MMC-25-410-9 29 M O (+)
10 MMC-25-410-10 34 F B (+)
11 MMC-25-410-11 36 M A (+)
12 MMC-25-410-12 37 F AB (+)
13 MMC-25-410-13 55 M O (+)
14 MMC-25-410-14 41 M B (+)
15 MMC-25-410-15 38 F B (+)
16 MMC-25-410-16 33 M AB (-)
17 MMC-25-410-17 28 M O (+)
18 MMC-25-410-18 43 M B (+)
19 MMC-25-410-19 45 M A (+)
20 MMC-25-410-20 30 F O (+)
21 MMC-25-410-21 29 M B (+)
22 MMC-25-410-22 35 M B (+)
23 MMC-25-410-23 22 M O (+)
24 MMC-25-410-24 42 M A (+)
25 MMC-25-410-25 21 M B (+)

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Cross Matching:

Sl. No Blood Unit No Age Sex Cross match Result

1 MMC-25-300-1 32 M Compatible

2 MMC-25-300-8 26 M Compatible

3 MMC-25-300-12 30 M Compatible

4 MMC-25-300-8 34 F Compatible

5 MMC-25-300-11 40 M Compatible

6 MMC-25-300-18 38 M Compatible

7 MMC-25-301-21 45 F Compatible

8 MMC-25-301-25 28 M Compatible

9 MMC-25-301-27 26 M Compatible

10 MMC-25-301-30 32 F Compatible

11 MMC-25-301-2 37 M Compatible

12 MMC-25-301-5 40 M Compatible

13 MMC-25-301-8 21 M Compatible

14 MMC-25-301-1 29 F Compatible

15 MMC-25-301-10 44 M Compatible

16 MMC-25-301-15 37 M Incompatible

17 MMC-25-301-13 20 M Compatible

18 MMC-25-302-5 32 F Compatible

19 MMC-25-302-12 37 F Compatible

20 MMC-25-302-2 20 M Compatible

21 MMC-25-302-9 29 M Compatible

22 MMC-25-302-8 32 M Compatible

23 MMC-25-303-10 34 F Compatible

24 MMC-25-303-11 36 M Compatible

25 MMC-25-303-14 41 M Compatible

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 INTERN FEEDBACK/COMMENTS ON INTERNSHIP

Year: 2025
Lab Section: Blood Bank & Transfusion Science, Haematology
Intern Name: Purabi Sahoo
Hospital Name: Debra Super Speciality Hospital
Duration and Dates: 12th may 2025 to 11th June 2025

• Name(s) of the supervisor under whom you were trained:

• Overview: Check (√) the explanation that most closely represents your evaluation of this section.

1.Was the intern’s responsibilities and privileges discussed with you?

Clearly discussed

Clear to some extent

Not clear

2. What is your opinion about training for interns in this section?

Excellent training
Good training
Adequately planned training
Poorly planned training

3.Do you feel that the responsibilities given to you in this section were according to your abilities to
handle them?
o The responsibilities given to me were suited to my ability to handle them.
o Some of the responsibilities were above my ability to handle them.
o The responsibilities given to me were too limited and too narrow.

4.Do you feel that you gained maximum benefits from the training in this section?
Yes

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Supervision and Instruction: Please rate the section on each item below by circling the appropriate
number on the rating scale.
The rating scale is: 0=Not applicable 1=Poor 2=Adequate 3=Above average 4=Excellent

Rating Scale

A Committed to the training program 0 1 2 3 4

B Instrument handling 0 1 2 3 4

C Encouraging intern learning 0 1 2 3 4

D Amount of feedback given to intern 0 1 2 3 4

E Friendliness toward interns’ questions 0 1 2 3 4

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 SUMMARY AND CONCLUSION
The list below the instruments/equipment you operated.
i. Blood grouping, Cross matching, Du test, Coombs test, Hb estimation, TTI, Blood shorting,
Requisition accepting.
ii. List the types of tests you observed but did not perform - Blood Transfusion, Donor Screening, Blood
storing.
iii. Academic/Clinical Correlation: By performing cross matching we can able to know the compatibility
of the receiver. By performing Du test, we can measure the level of weak D antigen in the blood. By
performing coombs test we can able to know the unnecessary antibody in blood. We have performed
Hb estimation to know the donors Hb concentration to donate blood. We have performed TTI we
prevent the transfusion diseases like HIV-1/2, Hepatitis-B/C, Syphilis, Malaria.

Note: Pls explain what correlation have you found between previously learned theories/concepts (at college)
and their practical application during training in this section. Recommendations would you like to make to
correlate your learning theories/concepts (at university) with the practical experience during training in this
section]

Name and Signature of The Intern:

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 OUTPUT OF TRAINING

Through Training, I can able to gain knowledge about the application of blood transfusion Coombs test, Du test, cross
matching, TTI’s etc.

Different research programs of human health are going in the concerned laboratory have been focused and we can able
to motivated to continue our higher education in the field and this visit is also a driving force to motivate as in research
work.

The role of discipline, the importance of devotion, and accountability for scientific work have been learned by us from
this training.

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 CONCLUSION

Medical laboratory or clinical laboratory is a laboratory where clinical pathology tests are carried out on
clinical Specimens to obtain information about the health of patient to aid in diagnosis, treatment and
prevention. of disease. Medical Laboratory Technology, is the branch of medical Science responsible for
performing laboratory investigations relating to the diagnosis, treatment and prevention of disease.

In this world especially in India, the population is growing fast and with that number of suffering people and
varieties of diseases are cropping up day by day. I opted for BMLT, to ease the suffering of people with
different types of disease.

In Ghatal Super Specialty Hospital, there is no limit of in the member of patients with different types of
diseases. There are many machines for specific learning to handle. I have learnt how to operate those machines,
to collect blood and different other specimens of the patients to help in diagnosis. The exact disease release by
them. Moreover, I have learnt how to handle the weak and diseased persons in my training. This knowledge
has changed my thoughts about the importance of BMLT. But I know that improvement of clinical Laboratory
is a must to save many more people. from different diseases and we have to go miles more to achieve our goal
in the field of Medical Laboratory technology.

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