M. Pharm.
2nd Semester, Makeup sessional, MPG-201T, Medicinal Plant Biotechnology
Max. marks 30, Time: 1 hr
PART A Multiple Choice questions Attempt all the questions (Each carries 1 mark)
1. The modification of exogenous compounds by plant cells is called
a) Biotransformation b) bioconversion c) both a and b d) biophytomodification
2. Transgenic organisms are
a) produced by gene transfer technology b) extinct organisms
c) naturally occurring and endemic d) produced by traditional plant breeding technique
3. In biotechnology, mass culturing of cells / microbes can be achieved by using
a) Test tube culture b) Bioreactor c) Autoclave d) electrophoresis
4. Bioreactors are used for
a) large production of desired substances by using cells /microbes scale
b) kill bacteria c) to store viruses d) to get chemicals
5. _________ and coils are used to maintain the temperature of the fermentation medium inside the
bioreactor.
a) Cooling Jackets b) Capacitors c) Transducers d) Electrochemicals
6. ________ is the part of a bioreactor helping in maintaining the homogeneity of the contents of the
bioreactor.
a) Agitator b) Fan c) Cooler d) Coolant
7. Which of the following is not a product of fermentation?
a) Oxygen b) Carbon dioxide c) Ethanol d) Lactate
8. Alcoholic fermentation is carried by yeast known as ___________
a) Lactobacillus b) Bacillus c) Saccharomyces cerevisiae d) Escherichia coli
9. In lactic acid fermentation, the final electron acceptor is:
(a) Lactic acid (b) Pyruvate (c) Oxygen (d) NAD
10. Identify the correct sequence during the industrial production of substances
a) Inoculation, screening, fermentation, downstream processing, and removal of waste
b) Screening, Inoculation, fermentation, downstream processing, and removal of waste
c) fermentation, screening, Inoculation, removal of waste, downstream processing
d) downstream processing, fermentation, screening, Inoculation, and removal of waste
PART B (Attempt any 2, each carries 5 marks)
1. Write a short note on chemically defined media of plant tissue culture
2. What is biotransformation? Explain bioreactors for pilot and large-scale cultures of plant cells
3. Write a note on the production of enzymes of pharmaceutical interest by fermentation
technology.
PART C (Attempt any 1, each carries 10 marks)
1. Enlist different types of bioreactors available in the market. Explain the conventional
bioreactor in detail. Mention its advantages.
2. Explain Fermentation / Bioprocess technology about the formation of secondary
metabolites.