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Biochemistry Spotting

The document lists various laboratory glassware and equipment used in biochemistry, detailing their specific functions and applications. Items include beakers, flasks, pipettes, and vials, each serving distinct purposes such as measuring, mixing, and storing solutions. Additionally, it mentions specific reagents and tests used for biochemical analysis, such as Benedict's solution and Fehling's solution for identifying reducing sugars.
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0% found this document useful (0 votes)
287 views5 pages

Biochemistry Spotting

The document lists various laboratory glassware and equipment used in biochemistry, detailing their specific functions and applications. Items include beakers, flasks, pipettes, and vials, each serving distinct purposes such as measuring, mixing, and storing solutions. Additionally, it mentions specific reagents and tests used for biochemical analysis, such as Benedict's solution and Fehling's solution for identifying reducing sugars.
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Biochemistry

spotting
1.) Glass Beaker: 7
used
for preparation and
mising
of solutions.
-

graduated
till50 ml.
-

2.) Conical
flask /Erlen-Meyer Hask!
-used for preparation and missing
-

of
sol

Glass >
3) cylinder:
measuring
sol
-> used to measure the
quantityof a

->
graduated
till 100m.

4.) White/Transparent ReagentBotth or


used
-> and Blore
to preserve bol"

reagents
~

5.) Brown Bottle. 7


Reagent
-> used to store reagentssensitive
to
light.
To prevent/protect from light exposure

6)Urine jar! 7
used -

to store wine
samples.

7.) Volumetaic
flask: >
-> used to prepare standard Imolar
solutions.

a) Glass pipette: O
S
-

transfer measured
used to

quantity of sol" from one


apparatus
to another.
9.) Glass Burette: -

>
used
for titration of a bo or
->

preparation of sol"
-

10.) Funnel: 7
sol",
- used
for filtration
of
to
apparatus
e to
transfer sol"
from a
bigger a

smaller apparatus.
1) I

11.) Urinometer: I

-> used to measure


specificgravity
wine.
of

12.) Glass rod/stirrer: -

-> used to mix/stir a solution

13.) Glass spalula :-


-

~ ~

used to
bransfer powdered substances
-

->
I

apparatus-another.
-

from one lo
has end with
-> a
flattened a
fissure
14) Metal spatula:- >
used bransfer powdered substances from
to
-
->
·

apparatusto another
one

->
used for powders thatreact withsilica

15.) (wette: /

density-OD/absorbance of
optical
-

-> used to measure sol"


~ itisused will
- machine.
calorimeter

16.) Test tube: - I


-> used
perform experiments
to
simulate
e
Not
-

17.) Testtube
holder:
< lab) in
-> used to hold test tube
10.) Auto pipette/Micro-pipetts-
to small measured
-> used transfer very
solution another
quantityof to
apparatus
-

from one

~ Ime -> 1000 as (heastcount - I all or 10%)

19.) Microtip/Pipette 7
tips, e
muropepell
solute transfer
used with
-> small
to very
measured
quantly of apparate
a
---
one

to another.

20.) Red vial (Plane vial or vacutainer.


>
-> used to
separate
serum from blood to
estimate hormones etc.
enzymes,

21. EDTA/ Purple vial or vacutainer. 7


-> used
for whole blood samples to estimate
HbA,c
-> used
for DNAisolation

22.) Flowride/Gray vial or vacutainer->


used estimation blood
for of fasting
-> -

sugar Post Prandial Blood sugar (PPBS),


I

Random Blood (RBS)


Sugar
23.) Cytrate/Blue vicl or vacutainer! 7
-> used to Prothrombin time
estimate and

International Normalised Ratio [PT/INR]

24.) Dessicator: -

7
-> used store
to
hygroscopic substances to prevent or
protectthem from moisture.

25.) Weighing
scale/Balance 7
-used to
weigh substances.

26.) pHmeter: >


used to measure
pH
-
27.) Magnetic stirrer: >
-> used to stir /mis a solution

20.) Colorimeter: - 7
-> used to measure optical density-OD & absorbance
of a

solution.

29.) I
Centrifuge:
->
separateserum
used to
from plasma.

30.) Jest tube stand: 7


I usedtokeep the test tubes.

Bath.
31.) Water
-> used to keep sol" in test tube inside
for incubation.
&

32.) Benedict's solution:


Composition: Copper
sulphate-sodium sodium
-> - +

[CuSOn) at
the

used to
->
identify (testfor reducing sugar
33.) fehling's solution A & B
~
Composition:- A=ag. sol"
of copper (II) sulphate (CuS04)
B sol" sodium potassium tartarate (KNa (yHu0s.4H20)
ag. of
=

Calso kla Rochelle's salt)


testfor
- used to
identify reducing sugar
34.) Ammonium sulphate
powder:-
-> used to detectketone bodies
in Rothera's test.

35.) Sulphur Powder:


used
=>
test for
Hay's sulphur bile salts.

36.) Eppendoo tube! - 7


isolated DNA in molecular
->

tostore biology

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