7.75295.0006-xxxxxxxxxx msp.
May 2021
www.sigmaaldrich.com
1.00617.0001 6. Preparation
• Analyze immediately after sampling.
• Check the sulfate content with the MQuant® Sulfate Test.
Spectroquant ®
SO42-
Samples containing more than 500 mg/l SO42- must be diluted with
distilled water.
Sulfate Cell Test • The pH must be within the range 2 - 10.
Adjust, if necessary, with sodium hydroxide solution or hydrochloric
acid.
• Filter turbid samples through a 0.45 µm membrane filter.
1. Method
Sulfate ions react with barium ions to form slightly soluble barium sulfate. 7. Procedure
The resulting turbidity is measured in the photometer (turbidimetric
method). Pretreated sample 2.0 ml Pipette into a reaction cell and mix.
The method is analogous to EPA 375.4, APHA 4500-SO42- E, and (20 - 40 °C)
ASTM D516-16. Reagent SO4-1K 1 level green Add, close the cell tightly, and shake
microspoon (in vigorously until the reagent is com-
2. Measuring range and number of determinations the cap of the pletely dissolved.
SO4-1K bottle)
Measuring range Number of determinations Leave to stand for exactly 2 min (reaction time), then measure the
sample in the photometer.
50 - 500 mg/l SO42- 25
Notes on the measurement:
For programming data for selected photometers / spectrophotometers see
www.sigmaaldrich.com/photometry. • For turbidimetric measurement the cells must be clean.
Wipe, if necessary, with a clean dry cloth.
3. Applications • The pH of the measurement solution must be within the range 1.0 - 2.0.
Sample material:
Groundwater and surface water, seawater 8. Analytical quality assurance
Drinking water and mineral water recommended before each measurement series
Wastewater To check the photometric measurement system (test reagent, measure-
Nutrient solutions for fertilization ment device, handling) and the mode of working, the sulfate standard
Soils after appropriate sample pretreatment solutions CRM (see section 5) or Spectroquant® CombiCheck 10 can be
Water for concrete in the construction industry used. Besides a standard solution with 100 mg/l SO42-, CombiCheck 10
also contains an addition solution for determining sample-dependent
4. Influence of foreign substances interferences (matrix effects).
Additional notes see under www.qa-test-kits.com.
This was checked individually in solutions containing 250 and 0 mg/l SO42-.
The determination is not yet interfered with up to the concentrations of Characteristic quality data:
foreign substances given in the table. Cumulative effects were not checked; For quality and batch certificates for Spectroquant® test kits see the
such effects can, however, not be excluded. website, where you will find all data in production control, that are
Concentrations of foreign substances in mg/l or % determined in accordance with ISO 8466-1 and DIN 38402 A51.
Ag +
10 Hg2+ 1000 S2O32- 25 EDTA 1000 9. Notes
Al3+ 1000 Mg2+ 1000 Zn2+ 1000 Hydrazine 1000
Ca2+ 250 Mn2+ 1000 Anionic • Reclose the reagent bottle immediately after use.
Cd2+ 1000 NH+ 1000 surfactants 100 • Information on disposal can be obtained at
CN- 1000 Ni2+ 1000 Cationic www.disposal-test-kits.com.
CO32- 1000 NO2- 1000 surfactants 500
Cr3+ 100 Pb2+ 100 Nonionic
Cr2O72- 100 PO43- 1000 surfactants 1000
Cu2+ 1000 S2- 25 Na-acetate 10 %
F- 1000 SiO32- 1000 NaCl 10 %
Fe3+ 1000 SO32- 100 NaNO3 0.1 %
5. Reagents and auxiliaries
Please note the warnings on the packaging materials!
The test reagents are stable up to the date stated on the pack when
stored closed at +15 to +25 °C.
Package contents:
1 bottle of reagent SO4-1K
25 reaction cells
1 sheet of round self-adhesive stickers for numbering the cells
Other reagents and accessories:
MQuant® Sulfate Test, Cat. No. 1.10019,
measuring range <200 - >1600 mg/l SO42-
MQuant® Universal indicator strips pH 0 - 14, Cat. No. 1.09535
Sodium hydroxide solution 1 mol/l Titripur®, Cat. No. 1.09137
Hydrochloric acid 1 mol/l Titripur®, Cat. No. 1.09057
Membrane filters 0.45 µm
Spectroquant® CombiCheck 10, Cat. No. 1.14676
Sulfate standard solution CRM, 125 mg/l SO42-, Cat. No. 1.25051
Sulfate standard solution CRM, 400 mg/l SO42-, Cat. No. 1.25052
Pipettes for pipetting volumes of 2.0 and 5.0 ml
The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in Merck KGaA, 64271 Darmstadt, Germany, Tel. +49(0)6151 72-2440
the U.S. and Canada. www.sigmaaldrich.com/photometry
EMD Millipore Corporation, 400 Summit Drive, Burlington MA 01803, USA,
© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. The vibrant M is a Tel. +1-978-715-4321
trademark of Merck KGaA, Darmstadt, Germany. All other trademarks are the property of their
respective owners. Detailed information on trademarks is available via publicly available resources. Sigma-Aldrich Canada Co. or Millipore (Canada) Ltd. 2149 Winston Park,
Dr. Oakville, Ontario, L6H 6J8, Phone: +1 800-565-1400
