SHENZHEN LIFOTRONIC TECHNOLOGY Co.

, Ltd

eCL8000 Installation Guide

CONTENTS
1 Product principles
2 Reagents
3 Installation Requirements
4 Preparation for installation

5 Product installation
01 Product principles
 Product principles

Ru(bpy)2+ e-

Terpyridine
Ru(bpy)3+ Ru(bpy)2+
Ruthenium
Voltage e-

TPA*
Buffer TPA H+ e-
Ru(bpy)2+
Tripropylamine
Product principles-Sandwich Assay

Antigen with
more epitope

Ruthenium-labeled
antibody

Biotinylated
antibody

Streptavidin-
coated micro bead

TPA Tripropylamine
 Product principles-Sandwich Assay

Antigen with
more epitope

Ruthenium-labeled
antibody

Biotinylated
TPA antibody

Streptavidin-
coated micro bead

TPA Tripropylamine
Product principles-Competitive Assay

Antigen with
less epitope

Ruthenium-labeled
antibody

Biotinylated
antigen

Streptavidin-
coated micro bead

TPA Tripropylamine
Product principles-Competitive Assay

Antigen with
less epitope

Ruthenium-labeled
antibody

Biotinylated
antigen

Streptavidin-
coated micro bead

TPA Tripropylamine
 Product principles-Competitive Assay

Antigen with
less epitope

Ruthenium-labeled
antibody

Biotinylated
TPA antigen

Streptavidin-
coated micro bead

TPA Tripropylamine
Product principles
Product principles
 Product principles

Controlled reaction system

Voltage
Use magnetic field to separate test objects
1. Add magnetic field to adsorb magnetic beads.
2. Add TPA to separate the immune complexes bound to
the magnetic beads.
3. Remove the factors that affect signal detection,
improve the sensitivity of detection results.

Apply voltage to initiate the reaction

[Link] and Terpyridine ruthenium are stable under
normal conditions, they will only be activated if voltage is
present.
02 Reagents
Reagents

2.1 System reagent(Auffer and Buffer)

Auffer Buffer

Application： Application:
Clean the aspiration probe, analytical liquid transfer test liquid, provide reaction material
line and measuring cell for luminescence reaction
Reagents

2.1 System reagent (System water)

System Water: Dilute Concentrated washing Buffer with distilled water to make system water.
(Ratio: 9L distilled water : 1L concentrated cleaning reagent).

Remember to add 9L distilled water first. Then add 1L

concentrated washing buffer.

Application:
Cleaning the S/R and aspiration probe.

Concentrated washing Buffer

Reagents

2.2 Immunoreagent
Contains 1 bottle of immunoreagent, QC card,
two bottle of calibrator, two bottle of QC.
(Some reagents only include calibrators)

Note:
[Link] consumption of each item of immune
reagents varies, please refer to the reagent
instructions.

Immunoreagent kit [Link] reagents cannot be stored upside

down to prevent reagent leakage.
Reagents

2.3 Maintenance reagent

Application: Maintenance of measuring cell

Measuring Cell Maintenance Buffer

 Reagents
2.4 Commissioning reagent

High pressure(voltage) adjustment reagent High pressure(voltage) adjustment Buffer

Application :
Calibration instrument consistency
03 Installation requirements
 Installation requirements

3.1 Space requirements

Ensure the required space for repair and maintenance. Considering the radiating of the instrument and the
liquid pipe behind the analyzer shall not squeezed, the installation of the analyzer should meet the
following requirements:
·The distance from the wall to left and right sides doors of the analyzer should more than 50cm;
·The distance from the wall to back door of the analyzer should more than 50cm;
·Make sure there is enough space on the work surface for the analyzer and reagent bucket.

墙
Wall

500mm
500mm 650mm 500mm

620mm
eCL 8000

150mm
 Installation requirements

3.2 Power requirements

·The analyzer must be used in a well-grounded condition. Before turning on the analyzer, make sure that
the input voltage meets the requirements of the instrument.

·The use of patch panels may introduce additional electrical interference and cause erroneous analysis
results. Please choose a place near the power outlet to avoid the use of patch panels.

• Voltage: A.C.100 ～ 240V

• Rated input power : 800VA
• Frequency: 50 / 60Hz
 Installation requirements

3.3 Environmental requirements

(1) Working temperature: 10 ℃ ～ 30 ℃

(2) Working humidity: ≦ 80%
(3) Atmospheric pressure: 75 kPa ～ 106 kPa
(4) The environment should be as clean as possible and without dust, mechanical vibration, large noise
sources and power supply interference.
(5) Do not place the device near sources of strong electromagnetic interference.
(6) Keep away from brush-type motors, flashing fluorescent lights and electrical contact devices.
(7) Choose a well-ventilated location to avoid direct pipeline or direct heat sources and fans.
04 Preparation for installation
 Preparation for installation

Accessory pack Unit

➢ Preparation tools Concentrated washing buffer 1 bottle

Buffer 1 bottle
Auffer 1 bottle
1. Common repair kit Incubation cups 5-6 league
2. 3# & 6# internal hexagon 10L bucket 2 PCS
3. 100-1000UL pipettor High voltage adjustment
buffer
1 bottle

4. Distilled water or deionized water, 9L+

5. Voltmeter
Cold chain package Unit
➢ Before installation, be sure to check whether the main
test reagent Each 1 box（100T）
box, accessory package and cold chain package are in
Measuring cell maintenance
place. 1 bottle
Buffer
High pressure adjustment
1 bottle
reagent
 Preparation for installation
4.1 Package comfirmation
1. Inspection before unboxing
Before removing the package for installation, please check whether the packing box is intact.

2. Unpacking steps
(1) Remove the outer box, cover, and take out the accessories;
(2) Remove the 16 M8 * 12 hex screws attached to the pallet on the instrument;
(3) Use a special hand to place the machine on the desktop;
05 Product installation
Product installation
1. 3D robotic arm

2. Special reagent cartridge

3. Reagent scanning region

4. Sample cartridge

5. Printer

6. Touch Screen

7. System reagent cartridge

8. Reaction tray

9. 2D robotic arm
Product installation

1. System water line connector

2. System water sensor connector

3. Waste liquid line connector

4. Waste liquid sensor connector

5. USB port

6. Ethernet port

7. Serial port

8. Power socket
 Product installation
5.1 Removal of fixtures
During transportation, the 2D arms and 3D arms need to be fixed to prevent collisions;
Use a No. 3 Allen wrench to remove the fixing plate. Be careful not to drop the screws into the
instrument. The fixing method is as follows:
1. Four hex screws to fix the Y axis of the 3D arm.
 Product installation

2. The Z axis of S/R probe is fixed with two hex screws;

3. The Z axis of aspiration probe is fixed with two hex screws;
4. The X axis of two arm is fixed with two hex screws.

2D-Arm Z axis 3D-Arm Z axis Two arm X axis

Application:Prevent damage to the 2D arm and 3D arm during

transportation
 Product installation

5. After removing all, there are 4 metal parts and 10 hex screws.
 Product installation
5.2 Install the reference electrode
1. Unplug the instrument power cable.
2. Remove the screws holding the right door of the instrument and remove the right door.
3. Remove the five screws on the cover of the detection unit and remove the cover.
(Before removing the cover, make sure that the whole machine is turned off. The photomultiplier in
the measuring cell is sensitive to light and if it is exposed to light when turned on, it will burn out the
photomultiplier.)
Product installation

4. Remove the plug by turning it counterclockwise.

5. Take out the reference electrode from the accessory
Reference electrode installation
Kit. instructions:
◼ Check whether the O-ring is on the
reference electrode and there is no
misalignment. Poor sealing of the
O-ring will cause water leakage;
◼ Soak the reference electrode
porous ceramic (near the O-ring)
in distilled water for 5 minutes
with the gold-plated plug side of
the circuit facing up and keep it
dry;
 Product installation

6. Plug in the reference electrode cable.

7. Close the detection unit cover and fix it.
8. Install and secure the right door of the instrument.

[Link] that the white

cable is tightly inserted and
will not fall off naturally.
[Link] the thread is plastic,
it should be lightly twisted
when screwing. Do not
damage the thread due to
misalignment.
Product installation
5.3 System water and accessary connection
1. Install system water (bottom) and waste liquid (bottom) according to the mark.
2. Connect power cable.

System water
System water
line connector
sensor connector

Waste Waste liquid

liquid line sensor
connector connector
Product installation
5.3 System water and accessary connection
The system water connector must be inserted into the bottom and locked firmly. Press the bottom and the
spring will bounce.

Note:
System water
System water
Poor system water
line connector
sensor connector connection will
cause initialization
failure

Waste Waste liquid

liquid line sensor
connector connector
Product installation
5.4 Loading system reagents
1. Load system reagents: Auffer and Buffer. Remove the reagent lid and cover the silicone lid.
2. Buffer is on the left with white lid. Auffer is on the right with black lid. Do not make mistakes
when closing the lid which will cause cross contamination.

(1) 2D robotic arm (2) Cleaning cell

(3) Buffer (4) Auffer
Product installation
5.5 Check before starting
[Link] that the system water bucket is filled with sufficient system water.

[Link] that the waste liquid bucket is empty.

[Link] that reagent, S/R probe and aspiration probe look good and are free of
dirt and bends.
[Link] that the system water and waste liquid pipes are not squeezed or bent.

[Link] that Auffer and Buffer are opened.

Product installation

5.6 Switch on

[Link] on the "Refrigeration" switch of the

instrument (located on the lower left side of
the instrument). That is the power switch of
the refrigeration system of the immune Host
reagent compartment.

2. Turn on the “host” switch of the instrument

(located on the lower left side of the
instrument). This is the power switch of the refrigerator
host.
Note: No sequence of switches.
 Product installation

5.7 Entering System

[Link] turning on the instrument switch,
the control software will automatically start
performing system initialization and self-
test.

[Link] the username (root) and password

(lifo5689) in the [Login] dialog box and
click [Login].

(This username is the highest authority for

engineer and cannot be easily notified to
unrelated people) Init
 Product installation
5.8 Loading and replacing the incubation cup
[Link] 25 incubation cups is a group and 100 incubation cups(4 groups) can be placed on the same time. If the
remaining volume shown on the [System] screen is insufficient or not loaded before starting the test, follow the
steps below to load or replace the incubation cups.
1.1 Open the front cover of the instrument, you can see the incubation cup loading area;
1.2 Select the area to be replaced or loaded on the interface; The cover plate of
1.3 The corresponding area of the incubation cups will be transferred to the loading area; the incubation cup
1.4 Take out used incubation cups, if not, skip this step; must be covered in
1.5 Place the new incubation cups in the loading area and click [Save]. the designated
1.6 If you need to replace or load cups in other areas, please repeat steps 2, 3, 4, 5. position and cannot
be shaken left and
right. Otherwise, S/R
probe hampered will
occur during the
incubation of the
sample.
 Product installation
5.9 Blank test

Enter “Advanced Maintenance”

→ “Performance Maintenance”
→ click “Blank Test” →
“Start”.

After the test, check the 10

signal values displayed in the
Buffer column, delete the first
value and check the average
result and CV.

The result CV should ＜ 5%

and the average result is within
the range of 200-1000.
 Product installation
5.10 High pressure adjustment

[Link] high-pressure adjustment reagent needs

to be placed in the Special reagent cartridge No.
3 reagent position. The Buffer reagent is
replaced with the high-pressure adjustment
BUFFER reagent.

2. Click 'High pressure Adjustment’ to start the

test. When the tested 10 signal values (without
the first value) are within the range of 100,000
± 5000 and the CV value is ＜ 3%, the high-
pressure adjustment is completed.
 Product installation
5.10 High pressure adjustment
3. If the high-pressure adjustment test signal is not in the range of 100000 ± 5000 and the retest is still
abnormal, check whether the reagent is contaminated or invalid first. If the reagent is normal, remove the top
cover of the instrument and find the potentiometer on the measurement unit. During the high-pressure
adjustment test, adjust the potentiometer corresponding to the line cap.
(One turn clockwise will decrease about 6000) After high pressure
adjustment, the incubation
cup position of A needs to be
replaced with a new one,
because the data of the cups
used for high pressure
adjustment will not be
recorded. If you need to do
high pressure adjustment in
daily use, you need to replace
the old incubation cups at
position A with a new one.
After the test, also remember
to replace new incubation
cups.
 Product installation
5.11 Calibration (Take PCT with installation reagent kit as an example)
1. Take out the PCT low value calibrator, PCT high value calibrator and PCT reagent from the PCT kit.
2. Scan PCT reagent card in RFID.
3. After scanning, the loading reagent window will pop up. Then load the reagent kit to any position of the
dedicated reagent unit, such as the 10th position, and there will be a beep when the reagent is placed.

PCT
 Product installation
5.11 Calibration (PCT)
4. After loading, it will display in the "Reagents" window. The magnetic beads will mix for 30 minutes.
(Because the mixing time is long, we can load the reagents first, and perform the blank test and high-pressure adjustment
during the mixing process.)
5. On the sample rack, place the PCT high value and PCT low value calibrators (high value in the front, low value behind),
and be sure to unscrew the bottle cap; As shown below, the sample rack is 1- 10 from left to right.
6. Push the sample rack with the calibrator to the bottom of the sample compartment, and there will be a clicking sound;
 Product installation
5.11 Calibration (PCT)
7. On the "Reagents" interface, select the reagent and click “Edit Calibration“. Click “Pick" to select the
corresponding low calibration and high calibration positions. Confirm that the remaining number of
incubation cups is sufficient to complete the calibration test.
8. Click "Save". After stirring the magnetic beads for 30 minutes, click "Start" test in the upper right corner;
Product installation
5.11 Calibration (PCT)
9. After the test is complete, click “Start Cal." on the "Reagents" interface, and click "Calibrate" to
complete the calibration;

Calibration pass conditions

◆ High and low value signal deviation

requirements: ± 70%;

◆ When the high and low value signal

deviation is positive and negative, the
high value minus the low value signal
deviation requires ± 30%;

◆ Test signal CV requirements: low value

<5%, high value <3%
 Product installation

5.12 QC (PCT)

1. After the calibration is

completed, scan the PCT QC card
in the RFID, the following
window will pop up automatically,
and click "Loading".
 Product installation

5.12 QC (PCT)
[Link] the PCT low value and
PCT high value QC on the sample
rack (there is no order requirement).
Be sure to unscrew the bottle cap.

3. Push the sample rack with the

QC to the bottom of the Sample
cartridge.

4. After loading, click on the

corresponding sample area in the
"Edit" interface first, and then click
“Test Edit“.
 Product installation

5.12 QC (PCT)
5. Click the place where the low-
QC located, select “QC”, select
the corresponding lot number for
the low QC, and select L (low
level) for the QC level. Do the
same operation of high-QC;

6. Select "OK" after you have

finished the edit.

7. Click "Start" to start the test.

 Product installation

5.12 QC (PCT)
After the test is completed,
the QC results will appear in
the QC interface

In this area, you can

choose different items,
high and low values,
test time, lots number
and other information.
 Product installation

5.13 Sample test

1. Place the sample on the
sample rack, S / R probe has
no puncture function, so
remove the sample cap;

2. Push the sample rack with

the sample to the bottom of
Sample cartridge; at the same
time, click "Patient
Information" to edit;

3. In the “Edit" interface,

click the corresponding
sample rack first, and then
click “Test Edit";
 Product installation

5.13 Sample test

4. Click the corresponding

location of the sample, select
"Sample" , then select the
sample type and number of tests
corresponding to the sample,
select PCT for the item, and
click "OK“.

5. Click "Start" to start the test.

 Product installation

5.13 Sample test

6. After the test is completed,

click the "Results" interface as
shown below to view the
sample test results.
THANK YOU！
Q/A