UNIVERSITY OF MADRAS
[Link]. DEGREE PROGRAMME IN MICROBIOLOGY
SYLLABUS WITH EFFECT FROM 2023-2024
Marks
Credits
Subject Inst.
Subject Name Category L T P S
Code Hours CIA Exter- Total
nal
Elective Generic/
Recombinant
336E5A Discipline Specific Y - - 3 4 25 75 100
DNA Technology
Elective- V
Course Objectives
CO1 Understand the principles of rDNA technology.
CO2 Illustrate the molecular tools employed in gene cloning.
CO3 Discuss the importance of various molecular techniques and their importance in Biotechnology.
CO4 Acquire knowledge about the concepts of tissue culture methods and transgenic organisms.
CO5 Examine recent trends in genetic engineering and its application in human welfare.
No. of Course
Unit Details
Hours Objectives
I Milestones in rDNA Technology-Gene manipulation-Steps involved in 12
Gene Cloning. Isolation of Chromosomal and Plasmid DNA. Restriction CO1
endonuclease - Discovery, Types, Mode of action-Application of Ligase,
DNA Polymerase, DNA Modifying enzymes and Topoisomerases. Uses
of Linkers and Adapters.
II Artificial Gene Transfer methods- Calcium Chloride Induction, 12 CO2
Electroporation, Microinjection, Biolistic method, Liposome and Viral-
mediated delivery. Cloning vectors –Properties and Applications - Plasmid
Based Vectors- Natural Vectors-pSC101 and [Link] Vectors-
pBR322 and pUC. Phage Based Vectors- Lambda phage. Hybrid Vectors,
Phagemid, Cosmid, BAC and YAC. Screening of Recombinants. Genomic
DNA and cDNA library-Construction and Screening.
III Molecular Tools- PCR- Types. Gel Electrophoresis- AGE and PAGE 12 CO3
Blotting Techniques - Southern, Western & Northern. DNA sequencing
methods - Sanger’s and Automated method. Recent trends in Genetic
Engineering- Targeted Genome Editing- ZFNs, TALENs, CRISPRs.
GeneTargeting-Knock-in &Knock-outs. DNA Finger printing,
IV Plant Biotechnology – Media, Growth regulators and Equipment for plant 12 CO4
tissue culture-Explant Culture- Micropropagation- Callus and Protoplast
Culture-Production of Bio-active secondary metabolites by plant Tissue
Culture - Agrobacterium and Crown Gall Tumors, Ti Plasmid and Ri
Plasmid – Animal Biotechnology-Principles of Animal Cell Culture,
Media and Equipment for Animal Cell Culture – Primary and Secondary
Cultures- Cell Lines- Types, Establishment and Maintenance of Cell
Lines.
V Applications of Genetic Engineering - Transgenic Animals – Mice and 12 CO5
Sheep-Recombinant Cytokines and their use in the Treatment of Animal
infections- Monoclonal Antibodies in Therapy- Vaccines and their
Applications in Animal Infections - Human Gene Therapy-Germline and
Somatic Cell Therapy -Ex-vivo Gene Therapy-SCID (Severe Combined
UNIVERSITY OF MADRAS
[Link]. DEGREE PROGRAMME IN MICROBIOLOGY
SYLLABUS WITH EFFECT FROM 2023-2024
Immuno Deficiency) – In-vivo Gene Therapy- CFTR (Cystic Fibrosis
Transmembrane Regulator) –Vectors in Gene Therapy-Viral and Non-
Viral Vectors. Transgenic Plants– Bt Cotton, Bt Corn, Round Ready
soybean, Flavr Savr Tomato and Golden Rice.
Total 60
Course Outcomes
Course On completion of this course, students will;
Outcomes
CO1 Illustrate the steps involved in introduction and expression PO4, PO6, PO7, PO9
of foreign DNA into bacteria, animal and plants cells and
their screening.
CO2 Discuss the various cloning vectors and their applications. PO4, PO6, PO7, PO9
CO3 Assess the usage and advantages of molecular tools. PO4, PO6, PO7, PO9
CO4 Explain plant and animal tissue culture protocols and gene PO4, PO6, PO7, PO9
transfer mechanism.
CO5 Elucidate and understand the application of genetic PO4, PO6, PO7, PO9
engineering and gene therapy.
Text Books
1. Brown T.A.(2016). Gene Cloning and DNA Analysis. 7thEdition . John Wiley and Jones,
Ltd.
2. Dale J. W., Schantz M.V. and Plant N. (2012). From Gene to Genomes – Concepts and
Applications of DNA Technology. 3rd Edition. John Wileys and Sons Ltd.
3. Keya Chaudhuri (2013). Recombinant DNA technology. The Energy and Resources
Institute
4. Siddra Ijaz, Imran UlHaq (2019). Recombinant DNA Technology. Cambridge Scholars
Publishing.
5. Monika Jain (2012). Recombinant DNA Techniques: A Textbook, I Edition,Alpha Science
International Ltd
References Books
1. Maloy S. R., Cronan J.E. Jr. and FreifelderD.(2011). Microbial Genetics. 2nd Edition.
Narosa Publishing Home Pvt Ltd.
2. Glick B. R. and Patten C.L.(2018). Molecular Biotechnology – Principles and Applications
of Recombinant DNA. 5th Edition. ASM Press.
3. Russell P.J. (2010). iGenetics - A Molecular Approach, 3rd Edition. Pearson New
International Edition.
4. Synder L., Peters J. E., Henkin T.M. and Champness W. (2013). Molecular Genetics of
Bacteria,4th Edition. ASM Press Washington-D.C. ASM Press.
5. James [Link], Michael Gilman, Jan Witkowski, Mark Zoller (1992). Recombinant
DNA. Scientific American Books
UNIVERSITY OF MADRAS
[Link]. DEGREE PROGRAMME IN MICROBIOLOGY
SYLLABUS WITH EFFECT FROM 2023-2024
Web Resources
1 [Link]
2 [Link]
3 [Link]
4 [Link]
5 [Link]
Methods of Evaluation
Continuous Internal Assessment Test 25 Marks
Internal Assignments
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (KI) Simple definitions, MCQ, Recall steps, Concept definitions
Understand /
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Analyse (K4) Problem-solving questions, Finish a procedure in many steps, Differentiate between
various ideas, Map knowledge
Evaluate (K5) Longer essay/ Evaluation essay, Critique or justify with pros and cons
Create (K6) Check knowledge in specific or offbeat situations, Discussion, Debating or
Presentations
Mapping with Programme Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S L S S M S
CO2 S L S S M S
CO3 S L S S M S
CO4 S L S S M S
CO5 S L S S M S
