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My RTPCR

The report indicates that Mr. G Sravankumar G tested negative for SARS-CoV-2 (COVID-19) based on nasal and throat swabs collected on January 17, 2022. The test was performed using Real Time PCR, and the results were reported on December 2, 2022. The report includes information on Ct values and limitations of the test, emphasizing the need for clinical correlation.

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Tom Robert
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11 views1 page

My RTPCR

The report indicates that Mr. G Sravankumar G tested negative for SARS-CoV-2 (COVID-19) based on nasal and throat swabs collected on January 17, 2022. The test was performed using Real Time PCR, and the results were reported on December 2, 2022. The report includes information on Ct values and limitations of the test, emphasizing the need for clinical correlation.

Uploaded by

Tom Robert
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Name : Mr.

G SRAVAN KUMAR G Sample ID : 81002541


Reg. No : 0092104150012
Referred by : Dr. SELF SPP Code : SPL-SAP-052
Referring Customer : SELF Collected On : 17-Jan-2022 05:32 PM
ITDOSE INFOSYSTEMS PVT. LTD.

Primary Sample : Nasal and Throat Swabs Received On : 17-Jan-2022 08:08 PM


Sample Tested In : Viral Transport Medium Reported On : 02-Dec-2022 08:47AM
: Final Report
Client Address : GUNTUR Report Status

MOLECULAR BIOLOGY
Test Name Results Units Ref. Range Method

SARS-CoV-2 (COVID-19) RT PCR


SARS-CoV-2 (COVID-19) Negative NA Real Time PCR

ICMR Registration number for Covid -19 is: SAPALAPVLHT


Ct Value Result
Negative Negative results indicate the absence of SARS -CoV-2 in the specimen
Cts<=24 Strong positive reactions indicative of abundant target Viral RNA
Cts of 25 to
Positive reactions indicative of moderate amounts of Viral RNA
31
Weak reactions indicative of minimal amounts of target nucleic acid
Cts >=31
(Viral RNA) which could present an infection state
What is the threshold cycle or Ct value?:In a real time PCR assay a positive reaction is detected by accumulation of a fluorescent signal. The Ct (cycle
threshold) is defined as the number of cycles required for the fluorescent signal to cross the threshold (ie exceeds background level). Ct levels are inversely
proportional to the amount of target nucleic acid in the sample (ie the lower the Ct level the greater the amount of target nucleic acid in the sample).

Ct Values differ from kit to kit, lab to lab, Collection Process and transportaion conditions and other factors.
1. COVID-19 detection requires identifying 3 genes; however 2 genes sepcific to SARS-CoV 2 (COVID-19) are sufficient for a positive detection.
2. E-gene gene is for the detection of Sarbecovirus (Genus B-betacoronavirus) as a first line test. RdRp gene or N-gene are for detection of severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) and are the confirmatory tests.
Limitations of the Test: Performance of 2019-nCoV Real-Time RT-PCR Diagnostic Panel has only been established in upper and lower respiratory specimens
(nasopharyngeal or oropharyngeal swabs, sputum, lower respiratory tract aspirates, bronchoalveolar lavage, and nasopharyngeal wash/aspirate or nasal aspirate).

Correlate Clinically.

*** End Of Report ***

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