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UV-620
UV-Vis Spectrophotometer
UV-620 scanning UV-Vis spectrophotometer is a
double beam stand-alone model with fixed band-
width of 1.8nm, providing excellent performance
for measurements in the range of 190nm to
1100nm. It has a large graphic LCD screen for
menu and spectrum display. The large sample
compartment accommodates a wide range of cell
holders and accessories including sipper and pel-
tier system. Optional Advanced Windows software
can fully control the instrument and makes it very
versatile.
Specifications
Wavelength Range 190 - 1100nm
Bandwidth 1.8nm
Double beam (single monochromator)
Optical System
grating: 1200 lines/mm
Scanning Speed Hi, Med, Low (Max 3000nm/min)
Wavelength Accuracy ± 0.3nm
Wavelength Repeatability 0.2nm
Photometric Range 0 - 200%T, -0.3 - 3.0A, 0 - 9999Conc
Photometric Accuracy ± 0.3%T or ± 0.002A@1A
Stray Light 0.05%T @220nm and 360nm
Stability ± 0.001A/h @500nm
Display 5” graphic LCD (320 x 240 dots)
Baseline Flatness ± 0.001A (200 - 1000nm)
Standard Holder Installed Standard 10mm square cuvette holders
Light Source Tungsten halogen & Deuterium lamp
Data Output USB and parallel
Power 220 - 240VAC/50Hz or 110 - 120VAC/60Hz
Dimensions 600 x 450 x 200mm
Weight 22kg
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Features
● Large graphic LCD (320 x 240 dots) and easy operation
● Automatic wavelength scanning
● Stand-alone model with full functions available eliminating the need of a computer
● Pre-aligned design ensures easy lamp replacement
● Large sample compartment to accommodate various holders and accessories
● Real–time clock for date and time stamping of results
Stand-alone model has built-in software for full functions, thus eliminating
the need for optional Windows analysis software via a PC
Through the control panel, user can perform all operations by pressing the
keys and all the results and operation information are displayed on the
LCD. Available functions include photometry, quantitative, wavelength
scan, kinetics, DNA/protein, multi-wavelength and system utilities.
Functions
1. Main Menu and Basic Mode 2. Quantitative (Standard Curve)
There are seven items in Main Manu. In Basic Mode, Up to 10 standard solutions can be used to es-
Absorbance, %T or Concentration can be measured. tablish calibration equation to measure the con-
Units such as ug/mL, mg/mL, mg/L, g/L, mM/L, M/L centration of unknowns. There are four fitting
etc. may be selected. options: linear fit, linear fit through zero, square
fit and cubic fit.
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3. Wavelength Scan 4. Kinetics
The scan intervals are 0.1, 0.2, 0.5, 1, 2, 5nm, and Real time measurement of Abs. vs. time can be
Hi, Medium and Low scan speeds are available. used for time course scanning or reaction rate cal-
Scan speeds vary from 100 to 3000 nm/min. Wave- culations. Wait time and measurement time up to 12
lengths are scanned from high to low so that the hours may be entered with time intervals of 0.5, 1, 2,
instrument waits at high wavelength. Post-run ma- 5, 10, 30 seconds and 1 min. Post-run manipulation
nipulation includes re-scaling axes, curve tracking includes re-scaling, curve tracking and partial curve
and peak picking. selecting required for the rate calculation.
5. DNA/Protein 6. Multi-Wavelength
Concentration and DNA purity are calculated Up to 10 wavelengths can be entered, allowing
Absorbance ratios 260nm/280nm or 260nm/230nm the measurement of multiple wavelengths on a
with optional subtracted absorbance at 320nm series of samples.
DNA Concentration = 62.9 x A260 – 36.0 x A280
Protein Concentration = 1552 x A260 – 757.3 x A280
Other wavelengths and factors may be entered.
Optional Advanced Windows software
The Windows based PC application software takes the full functions of the stand-alone version plus more powerful
data processing and expanded data collecting and storage capability. It comes standard with PC models or is op-
tional to a stand-alone model.
Optional Accessories
There is a wide range of accessories including different cell holders, sipper and peltier system etc available to sat-
isfy the specific analysis requirements. Please refer to the spectrophotometer accessory section from our website.
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Packing list (stand-alone model)
Description Qty
Spectrophotometer 1
10 mm Optical Square Cuvette 1 set of 4
10 mm Quartz Square Cuvette 1 set of 2
Standard 10mm Cuvette Holders Installed
Dust Cover 1
User Manual 1
Power Cord 1
Order Information
Part No. Description Note
UV-620 UV/Vis Spectrophotometer Stand-alone model
UV-620PC UV/Vis Spectrophotometer PC model (with SS-300)
SS-300 Advanced Windows software Manual, CD, USB cable
MIOSTECH, INC. (for North America) MIOSTECH PTY LTD (for other region)
2201 US HWY 1, Ste 168, New Brunswick, NJ 08902, U.S.A. H129/1-7 Mann Street, Chatswood, NSW 2067, AUSTRALIA
E-mail: sales@[Link] E-mail: sales@[Link]
