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Microbiology 20240629064118

The document outlines the syllabus for the B.Sc. Microbiology Skill Enhancement Course on Disinfection and Sterilization at Gujarat University, effective from June 2023. It includes learning outcomes focused on microbial control and practical skills in disinfection and sterilization techniques. The syllabus is divided into two units: theoretical knowledge of disinfection and sterilization methods, and practical applications including aseptic techniques and sterilization procedures.

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hemaniaryan01
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32 views1 page

Microbiology 20240629064118

The document outlines the syllabus for the B.Sc. Microbiology Skill Enhancement Course on Disinfection and Sterilization at Gujarat University, effective from June 2023. It includes learning outcomes focused on microbial control and practical skills in disinfection and sterilization techniques. The syllabus is divided into two units: theoretical knowledge of disinfection and sterilization methods, and practical applications including aseptic techniques and sterilization procedures.

Uploaded by

hemaniaryan01
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Gujarat University

[Link]. Microbiology
Syllabus
First Year B. Sc. Semester
Microbiology
(Skill Enhancement Course)
Credits:02 (02 hrs/week, Total: 30 hrs)

Effective from June-2023 Paper Code: SEC-DAS-126


Paper Name: Disinfection and Sterilization

Learning Outcomes:

1. Provides basic knowledge of control of microorganisms.


2. Provides basic skills of disinfection and sterilization.
3. Helpful to those who works or wants to work in the field of general microbiology,
medical & paramedical sciences, pharmaceuticals, agriculture, tissue culture,
biotechnology, fermentation etc

Unit-1 Introduction to Disinfection and Sterilization Teaching hrs:


10
A Understanding of terms: Sterilization, Disinfection, Sanitization,
Antisepsis, Antimicrobial agent (Germicide, Bactericide, Bacteriostatic)
B Conditions/Factors influencing the effectiveness of antimicrobial agents
C Control of microorganisms by chemical agents: Phenolics, Alcohols,
Aldehydes, Halogens, Quaternary ammonium compounds, Sterilizing gases
(Ethylene oxide, Beta-propiolactone, Vaporized hydrogen peroxide)
D Control of microorganisms by physical agents: Moist Heat, Dry Heat, UV
Radiation, Gamma Radiation

Unit-2 Practicals Teaching hrs:


20
1 Disinfection of hands by washing with soap and water
2 Aseptic technique
a. Disinfection of table top/floor of the room by using Phenol/Lysol
b. Sterilization of inoculating wire loop, straight wire, tip of the forcep,
glass spreader, mouth of test tube & flask etc. by direct heating in
burner’s flame.
c. Demonstration of aseptic transfer of bacterial culture
3 Disinfection of skin of fingertips by using alcohol/tincture of iodine
4 Principle, working knowledge and operation of hot air oven and autoclave
5 Sterilization of glassware in hot air oven
6 Sterilization of nutrient broth in an autoclave
References:
1. Prescott, Harley and Klein’s Microbiology, JM Willey, LM Sherwood, CJ Woolverton, 9h
Edition (2014), McGraw Hill Higher Education-USA
2 Microbiology, Pelczar JR., Chan ECS, Krieg NR, 5th Edition (1993), McGraw-Hill Book Company,
NY.
3. Principles of Microbiology, R. M. Atlas, 2nd Edition (Indian Edition) (2015) McGraw Hill
Education (India) Private Limited, New Delhi, India
4. Medical Microbiology – A Guide to The Laboratory Diagnosis and Control of Infection,
Edited by Robert Cruickshank, 11th Edition (1972), The English Language Book Society

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