Version: 1.

Revision date: 01.04.2022

Safety Data Sheet

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1 Product identifier

Trade name/designation: Methylene Blue Stain Sol. Aqueous STAIN

Product No.: M0236
Synonyms: none
CAS No. not applicable

1.2 Relevant identified uses of the substance or mixture and uses advised against
Relevant identified uses: For Laboratory, Research or Manufacturing Use.

1.3 Details of the supplier of the safety data sheet

Supplier
Avantor Performance Materials India Ltd.
Street 501, 5th floor, Tiffany Building, Hiranandani Business Park,
Postal code/City Thane, Maharashtra - 400607, India
Telephone 022-41288100

Emergency phone number

Telephone 1800105561

Preparation Information
Product Information Compliance
1.4 E-mail SDS@[Link]

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SECTION 2: Hazard identification

2.1 Classification of the substance or mixture

Physical hazards
Flammable liquid, category 3
Substance or mixture corrosive to metals, category 1

Health hazards
Skin corrosion, category 1B
Specific target organ toxicity (single exposure), category 3, narcotic effect

2.2 Label elements

Hazard pictograms

Signal word: Danger

Hazard statements
H226 - Flammable liquid and vapour.
H290 - May be corrosive to metals.
H314 - Causes severe skin burns and eye damage.
H336 - May cause drowsiness or dizziness.

Precautionary statements
Prevention:
P210 - Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P280 - Wear protective gloves/protective clothing/eye protection/face protection.

Response:
P301+P330+P331 - IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
P302+P352 - IF ON SKIN: Wash with plenty of water/…
P304+P340 - IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P305+P351+P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if
present and easy to do. Continue rinsing.
P308+P310 - IF exposed or concerned: Immediately call a POISON CENTER/doctor.

Storage:
P403+P235 - Store in a well-ventilated place. Keep cool.

2.3 Other hazards none

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SECTION 3: Composition / information on ingredients

Substances
not applicable

Mixtures
Composition / Information on ingredients
Substance name Identifier Concentration
Methylene blue CAS No.: 61-73-4 0.1-1%
2-Propanol CAS No.: 67-63-0 20-30%
Potassium hydroxide CAS No.: 1310-58-3 1-5%

SECTION 4: First aid measures

4.1 Description of first aid measures

General information
IF exposed: Immediately call a POISON CENTRE/doctor. If unconscious but breathing normally, place in
recovery position and seek medical advice. Never give anything by mouth to an unconscious person or a
person with cramps. Change contaminated, saturated clothing. Do not leave affected person unattended.
After inhalation
Immediately call a POISON CENTRE/doctor. Remove casualty to fresh air and keep warm and at rest. If
breathing is irregular or stopped, administer artificial respiration.
In case of skin contact
After contact with skin, wash immediately with plenty of water and soap. Remove contaminated, saturated
clothing immediately. Immediate medical treatment required because corrosive injuries that are not treated
are hard to cure.
After eye contact
In case of contact with eyes flush immediately with plenty of flowing water for 10 to 15 minutes holding
eyelids apart and consult an ophthalmologist. Protect uninjured eye. Remove contact lenses, if present and
easy to do. Continue rinsing.
In case of ingestion
Immediately call a POISON CENTRE/doctor. Do NOT induce vomiting. Rinse mouth thoroughly with water.
Give nothing to eat or drink.
Self-protection of the first aider
First aider: Pay attention to self-protection!

4.2 Most important symptoms and effects, both acute and delayed
no data available
4.3 Indication of any immediate medical attention and special treatment needed
no data available

SECTION 5: Firefighting measures

5.1 Extinguishing media

Suitable extinguishing media
Water spray

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 ABC-powder
Carbon dioxide (CO2)
Nitrogen

Extinguishing media which must not be used for safety reasons

no restriction

5.2 Special hazards arising from the substance or mixture

In case of fire may be liberated:
Pyrolysis products, toxic

5.3 Advice for firefighters

DO NOT fight fire when fire reaches explosives.
Special protective equipment for firefighters
Wear a self-contained breathing apparatus and chemical protective clothing.

5.4 Additional information

Do not allow run-off from fire-fighting to enter drains or water courses.
Do not inhale explosion and combustion gases.
Use caution when applying carbon dioxide in confined spaces. Carbon dioxide can displace oxygen.
Use water spray jet to protect personnel and to cool endangered containers.
In case of fire: Evacuate area.

SECTION 6: Accidental release measures

6.1 Personal precautions, protective equipment and emergency procedures

In case of major fire and large quantities: Remove persons to safety.
6.2 Environmental precautions
Discharge into the environment must be avoided.
6.3 Methods and material for containment and cleaning up
Spilled product must never be returned to the original container for recycling. Collect in closed and suitable
containers for disposal.
6.4 Additional information
Clear spills immediately.

SECTION 7: Handling and storage

7.1 Precautions for safe handling

All work processes must always be designed so that the following is as low as possible:
Inhalation
skin contact
Eye contact
Use extractor hood (laboratory).
If handled uncovered, arrangements with local exhaust ventilation have to be used.
If local exhaust ventilation is not possible or not sufficient, the entire working area must be ventilated by
technical means.
Wash hands before breaks and after work. Avoid contact with eyes and skin. When using do not eat, drink
or smoke. Provide eye shower and label its location conspicuously.

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7.2 Conditions for safe storage, including any incompatibilities
Recommended storage temperature: Keep bottles tightly closed and away from sources of ignition and
heat.
Keep container tightly closed and in a well-ventilated place. Keep/Store only in original container.
7.3 Specific end use(s)
Apart from the uses mentioned in section 1.2 no other specific uses are stipulated.

SECTION 8: Exposure controls/personal protection

8.1 Control parameters

Does not contain substances above concentration limits fixing an occupational exposure limit.

8.2 Exposure controls

Appropriate engineering controls

Technical measures and the application of suitable work processes have priority over personal protection
equipment. If handled uncovered, arrangements with local exhaust ventilation have to be used.

Personal protection equipment

Wear suitable protective clothing. When handling with chemical substances, protective clothing with CE-
labels including the four control digits must be worn.

Eye/face protection
Eye glasses with side protection

Skin protection
When handling with chemical substances, protective gloves must be worn with the CE-label including the
four control digits. Recommended glove articles DIN-/EN-Norms EN ISO 374 In the case of wanting to use
the gloves again, clean them before taking off and air them well.

By short-term hand contact

Suitable material: NBR (Nitrile rubber)
Thickness of the glove material: 0,12 mm
Breakthrough time: > 480 min

By long-term hand contact

Suitable material: NBR (Nitrile rubber)
Thickness of the glove material: 0,38 mm
Breakthrough time: > 480 min

Respiratory protection
no data available

Additional information
Wash hands before breaks and after work. Avoid contact with eyes and skin. When using do not eat, drink
or smoke. Provide eye shower and label its location conspicuously.

Environmental exposure controls

no data available

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SECTION 9: Physical and chemical properties

9.1 Information on basic physical and chemical properties

(a) Appearance
Physical state: liquid
Colour: no data available
(b) Odour: no data available
(c) Odour threshold: no data available

Safety relevant basic data

(d) pH: no data available

(e) Melting point/freezing point: no data available
(f) Initial boiling point and boiling range: no data available
(g) Flash point: no data available
(h) Evaporation rate: no data available
(i) Flammability (solid, gas): Flammable liquid and vapour.
(j) Flammability or explosive limits
Lower explosion limit: no data available
Upper explosion limit: no data available
(k) Vapour pressure: no data available
(l) Vapour density: no data available
(m) Density: no data available
(n) Solubility(ies)
Water solubility: no data available
(o) Partition coefficient: n-octanol/water: no data available
(p) Auto-ignition temperature: no data available
(q) Decomposition temperature: not applicable
(r) Viscosity
Kinematic viscosity: no data available
Dynamic viscosity: no data available
(s) Explosive properties: not applicable
(t) Oxidising properties: not applicable
(u) Particle characteristics does not apply to liquids

9.2 Other information

Bulk density: no data available
Refraction index: no data available
Dissociation constant: no data available
Surface tension: no data available
Henry's Law Constant: no data available

SECTION 10: Stability and reactivity

10.1 Reactivity
no data available

10.2 Chemical stability

The product is chemically stable under standard ambient conditions (room temperature).

10.3 Possibility of hazardous reactions

no data available

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10.4 Conditions to avoid
no data available

10.5 Incompatible materials

no data available

10.6 Hazardous decomposition products

no data available

SECTION 11: Toxicological information

11.1 Information on toxicological effects

Acute effects
Acute oral toxicity:
Methylene blue - LD50: > 1180 mg/kg - Rat - (RTECS)

2-Propanol - LD50: > 5045 mg/kg - Rat - (RTECS)

2-Propanol - LDLo: > 3570 mg/kg - Human - (RTECS)

Potassium hydroxide - LD50: > 273 mg/kg - Rat - (RTECS)

Acute dermal toxicity:

2-Propanol - LD50: > 12800 mg/kg - Rabbit - (RTECS)

Acute inhalation toxicity:

2-Propanol - LC50: 72600 mg/m3 - Rat - (Japan GHS Basis for Classification Data)

Irritant and corrosive effects

Primary irritation to the skin:
Causes severe skin burns and eye damage.

Irritation to eyes:
Causes serious eye damage.

Irritation to respiratory tract:

not applicable

Respiratory or skin sensitisation

In case of skin contact: not sensitising
After inhalation: not sensitising

STOT-single exposure
May cause drowsiness or dizziness.

STOT-repeated exposure
not applicable

CMR effects (carcinogenicity, mutagenicity and toxicity for reproduction)

Carcinogenicity
No indication of human carcinogenicity.

Germ cell mutagenicity

No indications of human germ cell mutagenicity exist.

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 Reproductive toxicity
No indications of human reproductive toxicity exist.

Aspiration hazard
not applicable

Other adverse effects

no data available

SECTION 12: Ecological information

12.1 Ecotoxicity

Fish toxicity:
2-Propanol - LC50: 9640 mg/l (96 h) - Brooke, L.T., D.J. Call, D.L. Geiger, and C.E. Northcott 1984. Acute
Toxicities of Organic Chemicals to Fathead Minnows (Pimephales promelas), Vol. 1. Center for Lake
Superior Environmental Stud., [Link] Wisconsin-Superior, Superior, WI :414

Daphnia toxicity:
2-Propanol - LC50: 1400 mg/l (48 h) - Blackman, R.A.A. 1974. Toxicity of Oil-Sinking Agents.
[Link]. 5:116-118

Algae toxicity:
no data available

Bacteria toxicity:
no data available

12.2 Persistence and degradability

no data available

12.3 Bioaccumulative potential

Partition coefficient: n-octanol/water: no data available

12.4 Mobility in soil:

no data available

12.5 Results of PBT/vPvB assessment

not applicable

12.6 Other adverse effects

no data available

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SECTION 13: Disposal considerations

13.1 Waste treatment methods

Appropriate disposal / Product

Dispose according to local legislation. Consult the appropriate local waste disposal expert about waste
disposal.

Appropriate disposal / Package

Dispose according to local legislation. Handle contaminated packages in the same way as the substance
itself.

SECTION 14: Transport information

Land transport (ADR/RID)

14.1 UN-No.: 2920

14.2 Proper Shipping Name: CORROSIVE LIQUID, FLAMMABLE, N.O.S.
(POTASSIUM HYDROXIDE/2-PROPANOL)
14.3 Class(es): 8 (3)
Classification code: CF1
Hazard label(s): 8+3
14.4 Packing group: II
14.5 Environmental hazards: No
14.6 Special precautions for user:
Hazard identification number (Kemler 83
No.):
tunnel restriction code: D/E
(Passage forbidden through tunnels of category D when
carried in bulk or in tanks. Passage forbidden through
tunnels of category E.)

Sea transport (IMDG)

14.1 UN-No.: 2920

14.2 Proper Shipping Name: CORROSIVE LIQUID, FLAMMABLE, N.O.S. (POTASSIUM
HYDROXIDE/2-PROPANOL)
14.3 Class(es): 8 (3)
Classification code:
Hazard label(s): 8+3
14.4 Packing group: II
14.5 Environmental hazards: No
Marine pollutant: No
14.6 Special precautions for user:
Segregation group: 18
EmS-No. F-E S-C
14.7 Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code
not relevant

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Air transport (ICAO-TI / IATA-DGR)

14.1 UN-No.: 2920

14.2 Proper Shipping Name: CORROSIVE LIQUID, FLAMMABLE, N.O.S.
(POTASSIUM HYDROXIDE/2-PROPANOL)
14.3 Class(es): 8 (3)
Classification code:
Hazard label(s): 8+3
14.4 Packing group: II
14.5 Special precautions for user:

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SECTION 15: Regulatory information

15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

SECTION 16: Other information

Abbreviations and acronyms

ACGIH - American Conference of Governmental Industrial Hygiensts
ADR - European Agreement concerning the International Carriage of Dangerous Goods by Road
AGS - Committee on Hazardous Substances (Ausschuss für Gefahrstoffe)
CLP - Regulation on Classification, Labelling and Packaging of Substances and Mixtures
DFG - German Research Foundation (Deutsche Forschungsgemeinschaft)
DNEL - Derived No Effect Level
Gestis - Information system on hazardous substances of the German Social Accident Insurance
(Gefahrstoffinformationssystem der Deutschen Gesetzlichen Unfallversicherung)
IATA-DGR - International Air Transport Association-Dangerous Goods Regulations
ICAO-TI - International Civil Aviation Organization-Technical Instructions
IMDG - International Maritime Code for Dangerous Goods
KOSHA - Korea Occupational Safety and Health Agency
LTV - Long Term Value
NIOSH - National Institute for Occupational Safety and Health
OSHA - Occupational Safety & Health Administration
PBT - Persistent, Bioaccumulative and Toxic
PNEC - Predicted No Effect Concentration
RID - Regulation concerning the International Carriage of Dangerous Goods by Rail
STV - Short Term Value
SVHC - Substances of Very High Concern
vPvB - very Persistent, very Bioaccumulative

Key literature references and sources for data

This Safety Data Sheet has been prepared based on information available for public as TOXNET information,
European Chemicals Agency (ECHA) substance dossier, papers from international cancer research institutes
(IARC Monographs), U.S. National Toxicology Program data, U.S. Agency for Toxic Substances and Disease
Control (ATSDR), PubChem websites and SDS from our raw material manufacturers.

Revision date Version Print date

01.04.2022 1.0 2022-04-01

Additional information

Indication of changes: none

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Disclaimer
The information provided in this Safety Data Sheet (SDS) was prepared based on data believed to be accurate
as of the date of this SDS. TO THE GREATEST EXTENT PERMITTED BY LAW, AVANTOR PERFORMANCE
MATERIALS (“AVANTOR”) EXPRESSLY DISCLAIMS ANY AND ALL REPRESENTATIONS AND
WARRANTIES REGARDING THE INFORMATION CONTAINED HEREIN INCLUDING, WITHOUT
LIMITATION, AS TO ACCURACY, COMPLETENESS, FITNESS FOR PURPOSE OR USE,
MERCHANTABILITY, NON-INFRINGEMENT, PERFORMANCE, SAFETY, SUITABILITY AND STABILITY. This
SDS is intended as a guide to the appropriate use, handling, storage and disposal of the product to which it
relates by properly trained personnel, and is not intended to be comprehensive. Users of Avantor’s products are
advised to perform their own tests and to exercise their own judgment to determine the safety, suitability and
appropriate use, handling, storage and disposal of each product and product combination for their own
purposes and uses. TO THE GREATEST EXTENT PERMITTED BY LAW, AVANTOR DISCLAIMS LIABILITY
FOR, AND BY USING AVANTOR’S PRODUCTS THE PURCHASER AGREES THAT UNDER NO
CIRCUMSTANCES SHALL AVANTOR BE LIABLE FOR, SPECIAL, INDIRECT, INCIDENTAL, PUNITIVE OR
CONSEQUENTIAL DAMAGE OF ANY TYPE OR KIND, INCLUDING WITHOUT LIMITATION, FOR LOSS OF
PROFITS, REPUTATIONAL DAMAGE, PRODUCT RECALL OR BUSINESS INTERRUPTION.

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