Valves

Installing a sample loop on a Rheodyne® manual

sample injection valve in Rohnert Park, CA

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Valves
High Pressure Valves

High Pressure Valves

Rheodyne® valves fit virtually any flow control application. There are
valves for preparative, analytical, nano, and microscale analysis in a
variety of flow configurations. Pressure ratings of the valves in this
chapter range from 125 psi (9 bar) to 15,000 psi (1,034 bar).

MX Series II™ Modules are actuated electronically and can be easily

adapted to existing instrumentation using contact closure, BCD, I2C,
USB, or used as stand alone devices. Industry standard sample injectors
and switching valves are designed for manual actuation. Locate the
valve module and flow configuration of choice using the table below.

Valve Configurations
Connecting
Valve Module Flow Configuration Tubing Size Page
MXT Modules: • 2-position, 10-port Switching 1/16” OD 127
Very High Pressure • 6-position, 7-port Selection
(<15,000 psi) • 2-position, 6-port Switching
MXP Modules: • 2-position, 6-port Switching 1/32” or 1/16” OD 127
High Pressure (analytical and nano scale)
(<6,000 psi) • 2-position, 6-port
Vertical Port Switching
• 2-position, 10-port Selection
(analytical and nano scale)
• 6-position, 7-port Selection
MXX Modules: • 2-position, 6-port Switching 1/16” or 1/8” OD 141
Low Pressure • 2-position, 6-port Double
(<125 psi) 3-Way Switching
• 6-position, 7-port Selection
• 10-position, 11-port Selection
Manual Sample • Dual Mode Analytical, Micro 0.020”, 1/16” or 1/8” OD 131 – 132
Injectors and Preparative Scale Injector
• Single Mode Analytical and
Micro Scale Injectors
Manual • 2-position, 6-port Switching 1/16” or 1/8” OD 133
Switching • 2-position, 6-port 3-Way and
Valves 4-Way Switching
• 6-position, 7-port Selection

Genuine Rheodyne valve accessories are also featured in this chapter.

Please see the pages indicated below for more information on these
valve consumables:

Vespel®, ETFE, and PEEK™ rotor seals; stainless steel, PEEK,

and propriety material stators (page 134)

Rheodyne RheBuild® Kits (page 135)

Stainless steel and PEEK sample loops (page 136 and 137)

Needle port accessories, mounting brackets, and the

IDEX Wrench (page 139 and 140)

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 Valves
High Pressure Valves

MXT Valves for Fast Chromatography MXP High Pressure Valves for HPLC
 alves for proprietary ultra-high performance applications
V Switching, selection, and injection valve models
Can withstand up to 15,000 psi (1,034 bar) C an withstand up to 6,000 psi (414 bar)
Made from combination UltraLife™ material Chemically compatible for use with most mobile
Available in a two-position and a six-position version phase compositions
Designed for use Rapid Replacement Pod™ for quick and A  vailable with analytical and nano-scale flow paths
easy maintenance

Please note: flow paths for the MX Series II™ modules can be viewed
Related Products on page 128.

 HP Fittings on pages 6 – 7
V
 ittings for Coned Ports on pages 10 – 17
F
 ittings for M4 Ports on page 12
F
Specifications
 ubing Sleeves to connect capillary tubing into 1/16” or 1/32” OD
T Flow Passages Nano: 0.10 mm (0.004”) diameter
Analytical: 0.25 mm (0.010”) diameter
ports on page 20
Power Requirements 100–120 VAC, 50-60 Hz
Regulatory Compliance CE Mark
Remote Control USB, I2C, BCD, Level Logic
Operating Temperature 0 °– 40 °C, non-condensing

Top Seller SEE STARRED PRODUCTS Storage Temperature 0 ° – 75 °C

Dimensions ( H x W x D) 117 mm x 76 mm x 128 mm (4.6” x 3.0” x 5.0”)

Wetted Rapid
Part No. Description Pressure Rating Connections Material Replacement Pod
MXT HPLC VALVES
MXT715-000 2-position, 6-port, Switching Valve, MX, TitanHT™, VHP 15,000 psi (1,035 bar) 10-32 ports for 1/16” OD Tubing UltraLife PD715-000
MXT715-102 2-position, 10-port, Switching Valve, MX, TitanHT, VHP 15,000 psi (1,035 bar) 10-32 ports for 1/16” OD Tubing UltraLife PD715-102
MXT715-105 6-position, 7-port, Selection Valve, MX, TitanHT, SEL 15,000 psi (1,035 bar) 10-32 ports for 1/16” OD Tubing UltraLife PD715-105
MXP HPLC VALVES
MXP7900-000 2-position, 6-port, Switching Valve, MX, TitanHP™ 6,000 psi (410 bar) 10-32 ports for 1/16” OD Tubing DuraLife®* PD7900
MXP7920-000 2-position, 6-port, Vertical Port Injector, MX, TitanHP, VP 6,000 psi (410 bar) 10-32 ports for 1/16” OD Tubing DuraLife PD7920
MXP7960-000 2-position, 10-port, Switching Valve, MX, TitanHP 6,000 psi (410 bar) 10-32 ports for 1/16” OD Tubing DuraLife PD7960
MXP7970-000 6-position, 7-port, Selection Valve, MX, TitanHP 6,000 psi (410 bar) 10-32 ports for 1/16” OD Tubing DuraLife II** PD7970
MXP7980-000 2-position, 6-port, Nano Switching Valve, MX, TitanHP 6,000 psi (410 bar) M4 ports for 1/32” OD Tubing DuraLife II PD7980
MXP7986-000 2-position, 10-port, Nano Switching Valve 6,000 psi (410 bar) M4 ports for 1/32” OD Tubing DuraLife II PD7986
MXP9900-000 2-position, 6-port, Biocompatible Switching Valve, MX, TitanHP, SEL 6,000 psi (410 bar) 10-32 ports for 1/16” OD Tubing PEEK™ PD9900
MXP9960-000 2-position, 10-port, Biocompatible Switching Valve, MX, TitanHP 6,000 psi (410 bar) 10-32 ports for 1/16” OD Tubing PEEK PD9960
* Duralife is a propriety material combination of SST and an advanced polymer.
**Duralife II is a proprietary material combination consisting of Titanium and an advanced polymer.

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Valves
High Pressure Valves

2 1 Application Note
1 2
The high pressure MX Series II can increase sample throughput and
3 6 laboratory productivity by automating HPLC applications. These applications
6 3 can be automated with the addition of the high pressure MX Series II valve to
an existing HPLC System, saving money and allowing for simple connections
4 5 with little set-up time.
5 4
Sample
Position 1 Position 2 Eluent Injector Column 2
Pump
Flow path of MX Series II™ Two-Position, Six-Port Switching Valve 1 10 9
2
3 8 Waste
7
Pump Pump 4 6
2 5 Detector
2
1 1
Regeneration Column 1
Pump
3 Waste 3 Waste Position A
Column 6 Column 6 Sample
Eluent Injector Column 2
Pump
4 Sample 5
5 4 1 10 9
2
Position 1 Position 2 3 8 Waste
7
Flow path of MX Series II Two-Position, Six-Port as an Injection Valve 4 6
5 Detector
Regeneration Column 1
Pump
1
2 Position B

6 Alternating column regeneration using

Rheodyne® Two-Position, Ten-Port MXP Module
3 7 (P/N MXP7960-000, page 127)

5
4

Flow path of MX Series II Six-Position, Seven-Port Selector Valve

1 10 1 10
9 9 Part No. Description Qty.
2 2
REPLACEMENT PODS
8 8 PD715-000 Rapid Replacement Pod™ for MXT715-100 ea.
3 3
PD715-102 Rapid Replacement Pod for MXT715-102 ea.
4 7 4 7 PD715-105 Rapid Replacement Pod for MXT715-105 ea.
5 6 5 6 PD7900 Rapid Replacement Pod for MXP7900-000 ea.
PD7920 Rapid Replacement Pod for MXP7920-000 ea.
Position 1 Position 2 PD7960 Rapid Replacement Pod for MXP7960-000 ea.
Flow path of MX Series II Two-Position, Ten-Port Switching Valve PD7970 Rapid Replacement Pod for MXP7970-000 ea.
PD7980 Rapid Replacement Pod for MXP7980-000 ea.
PD7986 Rapid Replacement Pod for MXP7986-000 ea.
PD9900 Rapid Replacement Pod for MXP9900-000 ea.
PD9960 Rapid Replacement Pod for MXP9960-000 ea.
REPLACEMENT FITTINGS
6000-209 Stainless Steel Standard Fittings, 10-pk
with 6000-210 Ferrules, 1/16”, 10-32
6000-282 RheFlex One-piece Fittings, 1/16”, 10-32, PEEK™, Natural 10-pk
6000-360 M4 RheFlex Fittings, 1/32”, PEEK, Natural 10-pk
FAST CHROMATOGRAPHY SAMPLE LOOPS
Part No. Description Pressure Rating Volume Qty.
7755-300 Stainless Steel Sample Loop 30,000 psi (2,070 bar) 5 µL ea.
7755-301 Stainless Steel Sample Loop 30,000 psi (2,070 bar) 10 µL ea.
7755-302 Stainless Steel Sample Loop 30,000 psi (2,070 bar) 20 µL ea.
7755-303 Stainless Steel Sample Loop 30,000 psi (2,070 bar) 50 µL ea.
7755-304 Stainless Steel Sample Loop 30,000 psi (2,070 bar) 100 µL ea.
REPLACEMENT FITTINGS
VHP-200x VHP Stainless Steel Fitting 30,000 psi (2,070 bar) 10-pk
for 1/16” OD tubing
VHP-320x VHP Reusable Fitting 25,000 psi (1,724 bar) 10-pk
for 1/16” OD tubing

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 Valves
Injector Valves

Sample Injectors
How to Choose a Sample Injector Scale, Sample Volume, and Loop Size
Table I below compares the characteristics of Rheodyne® manual Analytical scale models are for conventional columns with samples
sample injectors and will help you choose the most suitable model. from 1.0 µL to 5.0 mL. Microscale models are for 1.0 mm and 2.0 mm
inner diameter columns. Model 8125 has a sample range of 0.1 µL
Types and Capabilities to 500 µL, and can be used for both analytical and micro columns.
Preparative scale models are for columns with diameters from 1 to 10
Models ending in 25 (i.e. 7725) are dual mode injectors. Dual mode
cm, and operate at high flow rates with samples from 100 µL to 20 mL.
injectors can use both the partial-filling and the complete-filling
method for loading the sample loop (See the “Sample Loop Loading”
Application Note on page 131). They are variable volume injectors Liquid Contact Materials
because they allow the loading of various sample volumes. These dual All models have a polymeric rotor seal of Vespel® (pH 0 to 10 tolerance),
mode injectors, also called front-loading injectors, have a needle port ETFE or PEEK™ (both pH 0 to 14). Stators are 316 stainless steel or PEEK.
for loading sample built into the handle. The unique injection port Most models have an inert ceramic stator face assembly.
design allows the tip of the needle to connect directly to the sample
loop for no sample loss during loading. Make-Before-Break (MBB®)
Models ending in 10 (i.e. 7010) are single mode injectors. Single mode Models incorporating Rheodyne’s MBB architecture design provide
injectors use only the complete-filling method to load the sample loop. uninterrupted flow when switching between LOAD and INJECT
They are called fixed loop injectors as the sample loop size determines positions. MBB greatly reduces transient pressure shocks and is
the sample volume. These injectors require a Loop Filler Port accessory beneficial for flow-sensitive detectors, fragile columns, and pumps.
(page 139), as a needle port is not built into the valve handle. There Models 7725, 9725, 3725, and “i” versions contain the MBB design.
is not a direct connection between the syringe and the sample loop.
Therefore, an excess of sample must be used to overfill the Loop Filler Replacement Parts
Port and completely fill the sample loop. Genuine Rheodyne parts are available for easy maintenance of your
Models with an “i” suffix (i.e. 7725i) are identical to the models with the Rheodyne valve. RheBuild® Kits offer a complete solution to keep
same numbers but the “i” designates a built-in position sensing switch. your valve running, providing all necessary parts to rebuild your valve
The switch provides the chromatograph with a reproducible start signal and easy to use step by step instructions to assist you in the process.
to mark the injection time in the data system. Rheodyne also offers a complete line of sample loops, fittings, and
accessories designed specifically for the Rheodyne valve.
The reproducibility of manual sample injectors depends on operator
skill, syringe calibration, and the loading method. Partial-filling method
is typically reproducible to 1.0% relative standard deviation (RSD).
Complete-filling method is reproducible to 0.1% RSD for loops 5 µL.

Characteristics of Rheodyne Manual Sample Injectors

Partial Filling
Volumes Sample Loop Max. Max.
Type & Capabilities Scale (Range) Sizes (Range) Wetted Materials psi (bar)1 T (ºC) MBB2 Model3
Dual Mode Analytical 1 µL – 2.5 mL 2 µL – 5.0 mL 316 SST, Vespel 7,000 (483) 80º Yes 7725, 7725i
Can load the loop by two methods: 1 µL – 5.0 mL 2 µL – 10 mL PEEK, ETFE, ceramic 5,000 (340) 50º Yes 9725, 9725i
1) Partial filling – syringe determines volume without wasting sample Micro 0.1 µL – 500 µL 5 µL – 1.0 mL 316 SST, PEEK, Vespel, ceramic 7,000 (483) 80º No 8125
2) Complete filling – loop determines volume by over filling loop
Preparative 100 µL – 10 mL 2.0 mL – 20 mL 316 SST, PEEK 5,000 (340) 50º Yes 3725(i)-038
PEEK 4,000 (276) 50º Yes 3725, 3725i
Single Mode Analytical Not Applicable 5 µL – 5.0 mL 316 SST, Vespel 7,000 (483) 80º No 7000
Can load the loop by one method: 5 µL – 10 mL PEEK, ETFE, Ceramic 5,000 (340) 50º No 9010
Complete filling – loop determines volume by over filling loop Micro Not Applicable 0.5 µL – 5 µL 316 SST, Vespel 7,000 (483) 80º No 7410
0.2 µL – 1 µL 316 SST, Vespel 7,000 (483) 80º No 7520
SST = Stainless Steel
1
This is the maximum pressure to which the valve can be adjusted. Some models are shipped from the factory set for lower pressures.
2
MBB (Make-Before-Break) is a design that provides uninterrupted flow when switching between LOAD and INJECT. MBB also greatly reduces transient pressure shocks.
3
Models with an “i” suffix have a built-in position sensing switch. Models 8125 and 9010 also have a built-in switch.

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Valves
Switching Valves

High Pressure Switching Valves Effects of Valves and Tubing on Resolution

High pressure manual switching valves simplify procedures and The effect of tubing on analytical and microscale analyses can be
improve the speed, resolution, and sensitivity of HPLC analysis. The significant. Since dispersion caused by tubing is proportional to the
switching valves are available in 316 stainless steel and PEEK™, with a fourth power of diameter, large bore tubing should be avoided when
choice of 1.6 mm (1/16”) or 3.2 mm (1/8”) ports. See Table on page performing analytical scale or microscale analyses. Tubing ID size
132 for valve specifications. ≤ 0.25 mm (0.010”) is recommended.

Consider a system with injector and column switching valves

Column Selection
and analytical columns with small-bore connecting tubing. The
The six-position switching valves are used for column selection. These chromatograms below, made using a typical analytical chromatograph,
valves substitute one column for another without the need to manually show these effects. Scheme A is the control (injector column
disconnect the plumbing. This makes it easy to designate a separate detector) with no valve in the system. In Schemes B and C, two model
column for each analysis, which helps eliminate equilibration delays, 7060 Six-Position Switching Valves were placed side by side (injector
reduce interferences and prolong column life. Turning the valve handle valve #1 column valve #2 detector).
selects the column desired for a particular analysis. Columns switched
to off-line are automatically sealed at both ends. The injector and detector were connected to these valves by the same
tubing used in the control. The extra tubing pieces required to connect
Column Switching the valves to the column were a 10 cm length for valve #1-to-column,
and a 35 cm length for column-to-valve #2. The diameters of these
The two-position switching valves can be used to reroute mobile tubes are indicated in the experimental details, below.
phase during the chromatographic run without changing separation
techniques. They can also be used to perform sequential separations
with different columns and/or mobile phases.
Comparison of Observed Column Plates of
Although the model 7000 is the most commonly used and versatile Rheodyne® Analytical and MicroScale Injectors
switching valve, other models have specific uses such as for three-way 7725 8125
or four-way switching patterns. k’ = 0.6 2930 5054 72%
k’ = 1.5 4653 6904 48%
Many models have flow passages available in both standard bore and k’ = 7.9 7875 8305 5.0%
large bore (designated with an “L” suffix). L models use 1/16” fittings UV detector: 1 µL volume, 4 mm path. Sample volume: 2 µL, partial-filling method.
and tubing but have larger flow passage diameters than non-L models. Column: 2 mm ID x 100 mm long, 4 µm C-18. True plates of column = 11,570.
As such, L models can accommodate higher flow rates. Large bore
tubing can be used when the pressure drop must be limited. Large
bore valves have a lower pressure drop than standard bore valves when Effects of Valves and Tubing
both valve sizes accommodate the same flow rate. on Resolution
Column 2

Injector

Detector

Column 1

Position A

Column 2

Injector

Detector A Column Only B Valve w/ 0.007” C Valve w/ 0.020”

Tubing Tubing
Column 1
These chromatograms show the loss of resolution caused by the addition of
Position B two model 7060 column selection valves when using connection tubes of two
different inside diameters. Conditions for all cases: 4.6 mm x 12.5 cm column,
Column Selection Using a 2-Position, 6-Port Switching Valve
5 µm C-18 packing, 50% acetonitrile in water, 2.0 mL/min, 21 °C, 5.0 µL
sample partial filled into a model 7125 injector, 10 cm x 0.18 mm (0.007”)
bore injector outlet tube (to column or valve), 10 cm x 0.18 mm bore
detector inlet tube (from column or valve), low dispersion 1.0 cm path UV
detector cell, 0.2 sec detector time constant. See text above for details.

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 Valves
Manual Injector Valves

High Pressure Dual Mode Sample Injectors

Models 7725(i), 9725(i), 8125, and 3725(i)-038
 16 stainless steel
3
Application Note
Available in analytical, micro, and Dual Mode Sample Loop Loading:
preparative scale Partial-Filling vs. Complete-Filling
Make-Before-Break (MBB®) architecture
allows continuous flow between LOAD Partial-Filling
and INJECT positions which greatly Use the partial-filling method if you need to conserve sample, or if you want
reduces transient pressure shocks that to vary sample volume frequently.
disrupt your system
In partial-filling, the syringe sets the volume injected onto the column.
Wide, 30° port angles offer easier There is no sample waste, and the volume injected onto the column is equal
access to fittings using the to that dispensed from the syringe. Reproducibility is 1.0% relative standard
IDEX Wrench (Part No. 6810 on deviation (RSD). The volume of the sample loaded is limited to half the
page 140) sample loop volume. For example, the most you can load into a 200 µL
Front-end pressure screw sample loop is 100 µL.
makes it easy to adjust and Complete-Filling
maintain pressure Use the complete-filling method if you have plenty of sample, if you do not
A built-in position sensing vary sample volume, or if you need high reproducibility.
switch (“i” versions) provides the In complete-filling, the loop sets the volume loaded onto the column. Use
chromatograph with a reproducible start signal excess sample (two to five loop volumes) to replace all the mobile phase
The MBB valve design is illustrated below. In the LOAD position, mobile in the loop. See Figure 2. Change the loop to vary the sample volume.
Reproducibility is typically 0.1% RSD for loop sizes ≥ 5 µL. Accuracy is limited
phase flow from pump port to column port travels through both the
as loop volumes are nominal.
rotor seal groove and the MBB passage (Position A). As the rotor seal
grooves rotate to change from LOAD to INJECT, there is continuous Q: “
 Which method should I use and which Rheodyne® sample injectors use
mobile phase flow through both one rotor seal groove and the MBB this method?”
passage (Position B) until the rotation stops and both rotor seal grooves A: T
 here are two types of injectors available: dual mode and single mode.
are connected by the loop. Sample flow begins through the loop to Dual mode injectors allow both partial- and complete-filling whereas
the column just as all flow stops through the MBB passage (Position single mode injectors allow only complete-filling. See Sample Injectors
on pages 129 – 132.
C). Sample flow never enters the MBB passage. Valve flow passages
are 0.6 mm (0.024”) in diameter. If you are collecting experimental data, sample is scarce, and/or you want
to use different sample volumes, a dual mode injector with a large volume
sample loop is appropriate. Only dual mode injectors allow the partial-
filling method for easily varying your volumes (up to half your sample loop
volume) by setting the syringe volume. Once you begin routine analysis,
and/or you have an abundance of sample, either a dual mode or single
mode injector is appropriate. Both types of injectors allow the complete-filling
Make-Before-Break Design method in which you overfill the sample loop. Complete-filling maximizes the
reproducibility of your results.
Flow paths of model 7725(i) and 9725(i) with MBB design
Waste Waste
Shaft End Rotor Seal Grooves Rotor Seal Grooves
6 6
Rotor Seal Rotor Seal
Groove 1 Waste 1 Waste
Stator
Face Stator Face 5 5
Stator Seal Needle Port
2 2
Pump MBB Passage Column MBB Passage MBB Passage 4 Needle Port 4
Pump 3 Pump 3
Position A Position B Position C

Column Column

Top Seller SEE STARRED PRODUCTS LOAD INJECT

Flow path for the typical dual mode injector

Part No. Description Pressure Rating Sample Loop Included Tubing/Fittings Size Wetted Material
HIGH PRESSURE MANUAL INJECTORS
3725 Preparative Scale Dual Mode Injector 4,000 psi (276 bar) 10 mL 5/16-24 ports for 1/8” Tubing PEEK™
3725-038 Preparative Scale Dual Mode Injector 5,000 psi (345 bar) 10 mL 5/16-24 ports for 1/8” Tubing Stainless Steel
3725i Preparative Scale Dual Mode Injector with Switch 4,000 psi (276 bar) 10 mL 5/16-24 ports for 1/8” Tubing PEEK
3725i-038 Preparative Scale Dual Mode Injector with Switch 5,000 psi (345 bar) 10 mL 5/16-24 ports for 1/8” Tubing Stainless Steel
7725 Analytical Scale Dual Mode Sample Injector 5,000 psi (345 bar) 20 µL 10-32 ports for 1/16” OD Tubing Stainless Steel
7725i Analytical Scale Dual Mode Sample Injector, with Switch 5,000 psi (345 bar) 20 µL 10-32 ports for 1/16” OD Tubing Stainless Steel
8125* Micro Scale Dual Mode Sample Injector with Switch 5,000 psi (345 bar) 5 µL 10-32 ports for 0.020” (0.5 mm) or 1/16” Tubing Stainless Steel
9725 Analytical Scale Dual Mode Sample Injector 5,000 psi (345 bar) 20 µL 10-32 ports for 1/16” OD Tubing PEEK
9725i Analytical Scale Dual Mode Sample Injector with Switch 5,000 psi (345 bar) 20 µL 10-32 ports for 1/16” OD Tubing PEEK
*The 8125 requires special ferrules for 0.020” (0.5 mm) tubing. 8125-084 – 0.5 mm ferrule for 8125; 8125-086 – 0.5 mm ferrule for 8125 - 4-pk.

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Valves
Manual Injector Valves

High Pressure Single Mode Injectors

and Switching Valves
Models 7000(L), 7010, 3000-038, 3000 and 9010
 nalytical and preparative scale available
A
Stainless steel and PEEK™ materials
Applicable for sample injection, column switching, and sample
enrichment applications
Field adjustable up to 7,000 psi (models 7000 and 9000 only)

Sample Sample
2 2
1 1

3 Column 3 Column
6 Waste 6
Waste

4 4
5 5
Pump Pump
Position 1 Position 2
Pressure Drop vs. Flow Rate
Flow path of Two-Position, Six-Port Injection Valve
1000

100

10
Pressure Drop (psi)

0.1

.01
1 10 100 1000

Flow Rate (mL/min)

Pressure drop vs. flow rate for model 7000 and model 7000L (large-bore) valves;
water at 20 °C. Experimental measurements: The flow channel is one stator
inlet port, one rotor seal groove, one stator outlet port and two connecting
tubes. Solid squares = (1.0 mm 7000L valve) + (two 1.0 mm x 5.0 cm tubes).
Open squares = (0.6 mm 7000 valve) +(two 1.0 mm x 5.0 cm tubes).
Cross mark = (0.6 mm 7000 valve) + (two 0.5 mm x 5.0 cm tubes).
Solid lines are theoretical values for 10 cm long tubes of 1.0 mm
and 0.5 mm ID. Pressure drop is in units of psi.

Specifications
Model Stator Passage Diameter Factory Set Pressure Maximum Field Set Pressure Maximum Temperature (ºC)
3000, 3030 (PEEK) 1.0 mm (0.040”) 3,000 psi (207 bar) 4,000 psi (276 bar) 50º
3000-038 1.0 mm (0.040”) 4,000 psi (276 bar) 5,000 psi (340 bar) 50º
7000, 7010 0.6 mm (0.024”) 5,000 psi (340 bar) 7,000 psi (483 bar) 80º
7000L 1.0 mm (0.040”) 3,000 psi (207 bar) 5,000 psi (340 bar) 80º
7060L 1.0 mm (0.040”) 3,000 psi (207 bar) 5,000 psi (340 bar) 80º
SST = Stainless Steel

Part No. Description Tubing/Fitting Size Wetted Material

HIGH PRESSURE SINGLE MODE INJECTORS AND SWITCHING VALVES
3000 2-position, 6-port Switching Valve, Preparative Scale 5/16-24 Ports for 1/8” Tubing PEEK
3000-038 2-position, 6-port Switching Valve, Preparative Scale 5/16-24 Ports for 1/8” Tubing Stainless Steel
7000 2-position, 6-port Switching Valve, Large Bore 10-32 Ports for 1/16” OD Tubing Stainless Steel
7000L 2-position, 6-port Switching Valve, Large Bore 10-32 Ports for 1/16” OD Tubing Stainless Steel
7010 2-position, 6-port Single Mode Injector* 10-32 Ports for 1/16” OD Tubing Stainless Steel
9010 2-position, 6-port Switching Valve Single Mode Injector* 10-32 Ports for 1/16” OD Tubing PEEK
*Ships with a 20 µL sample loop attached to ports 1 and 4.

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 Valves
Manual Switching Valves

High Pressure Two- and Six-Position

Switching Valves
Models 7030(L), 7040(L), 7060(L),
3030-038, 9030, 9060, and 3030 Application Note
 vailable in 3, 4, and 6 way configurations
A Six column selection using two model 7060 switching valves.
Pressure rating 4,000 psi to 7,000 psi
(maximum field set pressure) Pump
Stainless steel and PEEK™ materials
Analytical and preparative scale available Sample
Injector

1
2
6

7 5
3
1 1 4
2 2
6 6
1 2 3 4 5 6
3 3
5 5
4 4
Position A Position B 1
2 6
Flow path of Three-Way Switching Valve

5
3
4
6 6
1 1 Detector
5 5
2 2
4 4
3 3 Please Note: The valves on this page ship with one set of 10-32 (1/16”)
or 5/16-24 (1/8”) RheFlex Two-Piece Fittings. The material of these
Position A Position B accessories match that of the stator material. Replacements and
Flow diagram of a Four-Way Switching Valve alternatives are available on pages 13 and 14.

Specifications
Part No. Stator Passage Diameter Factory Set Pressure Maximum Field Set Pressure Maximum Temperature (ºC)
3030 (PEEK) 1.0 mm (0.040”) 3,000 psi (207 bar) 4,000 psi (276 bar) 50º
3030-038 (SST) 1.0 mm (0.040”) 4,000 psi (276 bar) 5,000 psi (340 bar) 50º
7030, 7040 (SST) 0.6 mm (0.024”) 5,000 psi (340 bar) 7,000 psi (483 bar) 80º
7030L, 7040L (SST) 1.0 mm (0.040”) 3,000 psi (207 bar) 5,000 psi (340 bar) 80º
7060 (SST) 0.4 mm (0.016”) 5,000 psi (340 bar) 7,000 psi (483 bar) 80º
7060L (SST) 1.0 mm (0.040”) 3,000 psi (207 bar) 5,000 psi (340 bar) 80º
9030, 9060 (PEEK) 0.4 mm (0.016”) 5,000 psi (340 bar) 5,000 psi (340 bar) 50º
SST = Stainless Steel

Part No. Description Configuration Tubing/Fitting Size Wetted Material

HIGH PRESSURE SWITCHING VALVES
3030 2-Position, 6-Port Switching Valve, Preparative Double 3-Way 5/16-24 ports for 1/8” OD Tubing PEEK
3030-038 2-Position, 6-Port Switching Valve, Preparative Double 3-Way 5/16-24 ports for 1/8” OD Tubing Stainless Steel
7030 2-Position, 6-Port Switching Valve Double 3-Way 10-32 ports for 1/16” OD Tubing Stainless Steel
7030L 2-Position, 6-Port Switching Valve, Large Bore Double 3-Way 10-32 ports for 1/16” OD Tubing Stainless Steel
7040 2-Position, 6-Port Switching Valve, Large Bore 4-Way 10-32 ports for 1/16” OD Tubing Stainless Steel
7040L 2-Position, 6-Port Switching Valve 4-Way 10-32 ports for 1/16” OD Tubing Stainless Steel
7060 6-Position, 7-port, Switching Valve 6-Way 10-32 ports for 1/16” OD Tubing Stainless Steel
7060L 6-Position, 7-Port, Switching Valve, Large Bore 6-Way 10-32 ports for 1/16” OD Tubing Stainless Steel
9030 2-Position, 6-Port Switching Valve Double 3-Way 10-32 ports for 1/16” OD Tubing PEEK

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Valves
Rotor Seals and Stators

Rotor Seals and Stators

The rotor seal is the polymeric disc that makes a high pressure seal
against the stator or stator face seal. The seal wears with use and is
one of the only parts that may
need routine replacement. Part No. For Valve Model No. Description
ETFE BLEND ROTOR SEALS
Stators are available in 316
7000-017 7000L, 7040L ETFE Rotor Seal
stainless steel, PEEK™ and 7010-071 7010, 7010-087, 7000, 7040 ETFE Rotor Seal
proprietary materials. Stators 7030-015 7030 ETFE Rotor Seal
need replacement only if the 7060-074 7060, 7066, 9060 ETFE Rotor Seal
ports or sealing surfaces 7060-067 7060L ETFE Rotor Seal
become damaged. Avoid 7125-079 7125, 7125-081, 7725 ETFE Rotor Seal
damage from use of improper 7410-075 7410 ETFE Rotor Seal
injection needles by referring 8125-097 8125 ETFE Rotor Seal
to the “Using Proper Syringe 9010-051 9010 ETFE Rotor Seal
Needles” Application Note 9125-082 9125, 9725 ETFE Rotor Seal

on page 139. PEEK BLEND ROTOR SEALS

3030-005 3030, 3030-038 PEEK Rotor Seal
Please Note: Rotor seals for MX Series II™ Modules are available in 3710-008 3000, 3000-038, 3710, 3710-038 PEEK Rotor Seal
RheBuild ® Kits on page 135. Stators for MX Series II Modules are available 3725-018 3725, 3725-038 PEEK Rotor Seal
on this page. MX (Series I) Module rotor seals are available in RheBuild 9010-065 7010, 9010 PEEK Rotor Seal
8125-119 8125 PEEK Rotor Seal
Kits on page 135. Stators are available at www.idex-hs.com.
9125-095 9125, 9725 PEEK Rotor Seal
STATORS FOR MX SERIES II MODULES
7123-548 MXT715-000 Stator
7123-550 MXT715-105 Stator
7123-568 MXT715-102 Stator
7770-229 MXP7920-000 Stator
7980-004 MXP7980-000 Stator
7986-004 MXP7986-000 Stator
7900-146 MXP9900-000 Stator
7900-179 MXP7900-000 Stator

Application Note 7900-183

7960-014
MXP7970-000
MXP7960-000
Stator
Stator
9960-002 MXP9960-000 Stator
How to Select the Right Rotor Seal STATORS FOR OTHER RHEODYNE VALVES
The standard rotor seal in many 3725-006 3725, 3710-038, 3000-038 and 3030-038 Stator
Rheodyne® manual valves is made 3725-085 3725-038, 3710-038, 3000-038 and Stator
from a Vespel® blend. This polyimide 3030-038
has low wear and high chemical 7010-069 7000L, 7030L, 7040L Stator
Vespel ETFE PEEK
resistance. Vespel tolerates a pH 7010-040 7010, 7125, 7000, 7030 and 7040 Stator
range of 0 to 10. Solutions more 7010-066 7125-081 and 7010-087 Stator
basic than pH 10 dissolve Vespel 7060-039 7060 and 7066 Stator
which damages the rotor seal. If you 7060-065 7060L Stator
use any solutions above pH 10, Rheodyne recommends a PEEK blend rotor 7123-047 PR/EV500-100 Stator
seal. PEEK offers a high chemical resistance and versatility, and will tolerate 7123-127 PR/EV750-107 Stator
the entire pH range from 0 to 14. ETFE blend rotor seals are appropriate for 7123-128 PR/EV700-107 Stator
use in applications where PEEK is not generally acceptable, such as when 7123-142 PR/EV500-104 Stator
methylene chloride or DMSO in higher concentrations is being used. 7123-145 PR/EV550-104 Stator
7123-147 PR/EV550-100 Stator
7123-148 PR/EV500-101 Stator
7123-149 PR/EV550-101 Stator

Top Seller SEE STARRED PRODUCTS

7123-180 PR703-100 and EV700-105 Stator
7123-221 PR753-100 and EV750-105 Stator
7123-223 PR/EV700-112 Stator
7123-390 EV200-102 Stator
Part No. For Valve Model No. Description 7410-041 7410 and 7413 Stator
VESPEL BLEND ROTOR SEALS 7520-030 (inlet) 7520 Stator
7000-016 7000L, 7040L Vespel Rotor Seal 7520-035 (outlet) 7520 Stator
7010-039 7010, 7000, 7040 Vespel Rotor Seal 7650-002 PR/EV700-102 Stator
7030-003 7030 Vespel Rotor Seal 7725-010 7725(i) Stator
7030-014 7030L Vespel Rotor Seal 7750-070 7750 Stator
7060-070 7060, 7066 Vespel Rotor Seal 7750-038 PR/EV700-100 Stator
7060-064 7060L Vespel Rotor Seal 8125-098 8125 Stator
7125-047 7125, 7725 Vespel Rotor Seal 9060-016 9060 Stator
7410-038 7410 Vespel Rotor Seal 9125-043 9125, 9010, 9030 and 9725(i) Stator
7413-013 7413 Vespel Rotor Seal 9650-009 PR/EV750-102 Stator
8125-038 8125 Vespel Rotor Seal 9750-021 PR/EV750-100 Stator

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 Valves
Kits

RheBuild® Kits
RheBuild Kits are available for all Rheodyne® brand products. Included
in each individualized RheBuild Kit are all parts, tools, and instructions Order Online WWW. IDEX-HS .COM
to maintain precision performance of your particular product. RheBuild
Kits eliminate individual part ordering.

Part No. Description

RHEBUILD KITS FOR MX SERIES II™ VALVES
7150-999 RheBuild Kit for MXT715-000
7152-999 RheBuild Kit for MXT715-102
7155-999 RheBuild Kit for MXT715-105
7920-999 RheBuild Kit for MXP7920-000 and MXP7900-000
7960-999 RheBuild Kit for MXP9960-000
7961-999 RheBuild Kit for MXP7960-000
7970-999 RheBuild Kit for MXP7970-000
79801-999 RheBuild Kit for MXP7980-000

Application Note 79861-999 RheBuild Kit for MXP7986-000

7900-999 RheBuild Kit for MXP9900-000
RHEBUILD KITS FOR MANUAL VALVES
How to Avoid Pressure Transients
3725-999 RheBuild Kit for models 3725, 3725i, 3725-038, 3735i-038
Air in the sample loop can cause an instantaneous system pressure drop that 7010-996 Conversion Kit including Stator Face Assembly for model 7010
eventually returns to a normal level. Air causes the pressure to drop when the 7010-997 RheBuild Kit including Stator for model 7010
injector moves from the LOAD to the INJECT position. When large sample 7010-999 RheBuild Kit for model 7010 and 7010-type Valves
loops (≥100 µL) are partially loaded, air present in the needle port tube is 7125-999 RheBuild Kit for models 7125 and 7126
pushed into the sample loop (see Figure 1). Air can also enter the sample 7125Ti-999 RheBuild Kit for model 7125-081
loop from siphoning which occurs when the vent line is higher than the 7410-999 RheBuild Kit for model 7410
injection port. In either case, upon injection, the system pressure collapses 7520-999 RheBuild Kit for models 7520 and 7526
the air bubble, causing pressure to drop momentarily.
7725-999 RheBuild Kit for models 7725 and 7725i
A pressure drop in the system caused by air results in changes in retention 8125-999 RheBuild Kit for models 8125 and 8126
time, artifact peaks, and affects column performance. 9010-999 RheBuild Kit for model 9010
Avoid pressure drops by removing the air in the needle port tube. Do this 9125-999 RheBuild Kit for models 9125 and 9126
by flushing about 1 mL of mobile phase with a luer syringe with needle port 9725-999 RheBuild Kit for models 9725 and 9725i
cleaner. Keep the needle port tube filled with mobile phase by occasional RHEBUILD KITS FOR MX SERIES I™ VALVES
flushing. Adjust the vent line(s) so the outlet is at the same horizontal level as 7900-999 RheBuild Kit for models MX7900-000, MX7925-000,
MX9900-000, MX9925-000
the needle port (see Figure 2). For additional injection troubleshooting, refer
7960-999 RheBuild Kit for model MX7960-000
to the Rheodyne Troubleshooting Guide for HPLC Injection Problems. You
7980-999 RheBuild Kit for model MX7980-000
may download the Guide from the IDEX Health & Science web site:
7984-999 RheBuild Kit for model MX7984-000
www.idex-hs.com under Support.
7986-999 RheBuild Kit for model MX7986-000
RHEBUILD KITS FOR LABPRO™ & EV AUTOMATED FLUIDIC INSTRUMENTS
1001-999 RheBuild Kit for model PR100-101
Figure 1 Air present in the needle
port tube is pushed by the syringe 1005-999 RheBuild Kit for model PR/EV100-105
during loading into the sample loop 1006-999 RheBuild Kit for model PR/EV100-106
5001-999 RheBuild Kit for models PR/EV500-101 and PR/EV550-101
5100-999 RheBuild Kit for models PR/EV500-100 and PR/EV550-100
Air
5104-999 RheBuild Kit for models PR/EV500-104 and PR/EV550-104
Figure 2 Pathway of the flushing 7004-999 RheBuild Kit for models PR/EV700-104 and PR/EV750-104
mobile phase using the Needle Port 7112-999 RheBuild Kit for models PR/EV700-112 and PR/EV750-112
Cleaner, Part # 7125-054 (see page 7501-999 RheBuild Kit for models PR/EV700-100 and PR/EV750-100
139) when the injector is in INJECT
7502-999 RheBuild Kit for models PR/EV700-102 and PR/EV750-102
7507-999 RheBuild Kit for models PR/EV700-107 and PR/EV750-107
7531-999 RheBuild Kit for models PR703-100 and PR753-100

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Valves
Sample Loops

Stainless Steel Sample Loops

These high quality stainless steel sample loops have burr-free,
square-cut ends to ensure a flush connection to valve ports. The size
designations of loops are nominal. The actual volumes can differ
from the theoretical designations because of the 0.001” (± 0.025 mm)
tolerance of the metal tubing bore.

Accuracy of large metal loops (1.0 mm, 0.040” bore) is about ±5%,
intermediate loops (0.5 mm, 0.020” bore) ±10%, and small loops
(0.2 mm, 0.007” bore) ±30%.

Since both standards and unknowns are usually analyzed using the
same sample loop, knowledge of the actual, accurate volume is rarely
needed. If the sample loop volume must be known, it is best to calibrate
the loop in place on the valve so the flow passages in the valve are
also taken into account. An alternative to calibration is to use a dual
mode injector and partial-filling method of loading. See the “Sample
Loop Loading” Application Note on page 131.

Model 7725 Injector loops are not interchangeable with loops for the Part No. Volume Tubing
model 7125. The port angle for the 7725 is 30° whereas the port angle RHEODYNE® STAINLESS STEEL LOOPS FOR MXT715-000
for the 7125 is 20° requiring the loops to have a different shape. 7755-300 5 µL Sample Loop 0.18 mm (0.007”) ID x 1/16” OD
7755-301 10 µL Sample Loop 0.30 mm (0.012”) ID x 1/16” OD
Model 8125 Micro-Scale Sample Injector requires special loops in the
7755-302 20 µL Sample Loop 0.30 mm (0.012”) ID x 1/16” OD
5.0 µL to 50 µL range. The 8125 sample loops are made with 0.5 mm
7755-303 50 µL Sample Loop 0.51 mm (0.021”) ID x 1/16” OD
(0.020”) OD tubing. 7755-304 100 µL Sample Loop 0.51 mm (0.021”) ID x 1/16” OD
RHEODYNE STAINLESS STEEL LOOPS FOR 7125, 7010 INJECTORS
(DO NOT USE FOR 7725)
1876 10 mL Sample Loop 2.0 mm (0.080”) ID x 1/8” OD
Application Note 1877 20 mL Sample Loop 2.0 mm (0.080”) ID x 1/8” OD
7020 5 µL Sample Loop 0.18 mm (0.007”) ID x 1/16” OD
How to Properly Install Sample Loops 7021 10 µL Sample Loop 0.30 mm (0.012”) ID x 1/16” OD

Stainless Steel 7022 20 µL Sample Loop 0.51 mm (0.020”) ID x 1/16” OD

7023 50 µL Sample Loop 0.51 mm (0.020”) ID x 1/16” OD
Stainless steel sample loops are supplied with fittings that are not swaged
7024 100 µL Sample Loop 0.51 mm (0.020”) ID x 1/16” OD
onto the tube. It is important that the loop be completely bottomed in the
7025 200 µL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD
injector port before the ferrule is swaged onto the tube. The depth of the
7026 500 µL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD
tubing holes may vary slightly from port to port and from valve to valve. A
7027 1.0 mL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD
fitting made up in one port may leave a small cavity in another port. The
cavity causes high dispersion and peak distortion such as fronting, tailing, or 7028 2.0 mL Sample Loop 1.0 mm (0.040”) ID x 1/16” OD
broadening. It is good practice to label loop ends so they will be replaced 7029 5.0 mL Sample Loop 1.0 mm (0.040”) ID x 1/16” OD
in the same, respective ports that were used in swaging the ferrules. Hint: RHEODYNE STAINLESS STEEL LOOPS FOR 3725-038, 3725i-038 INJECTORS
swaging ferrules separately on each side, into each respective valve port 3065-018 2.0 mL Sample Loop 2.0 mm (0.080”) ID x 1/8” OD
makes loop installation easier. 3065-019 5.0 mL Sample Loop 2.0 mm (0.080”) ID x 1/8” OD
3065-023 10 mL Sample Loop 2.0 mm (0.080”) ID x 1/8” OD
3065-025 20 mL Sample Loop 2.0 mm (0.080”) ID x 1/8” OD
To install the sample loop:
RHEODYNE STAINLESS STEEL LOOPS FOR 7725, 7725i, PR/EV700-100,
a) Take one end of the loop and place the nut PR/EV703-100, MX MODULE INJECTORS (DO NOT USE FOR 7125)
(1) and ferrule (2) onto the tubing (3) with the 1876 10 mL Sample Loop 2.0 mm (0.080”) ID x 1/8” OD
threaded portion of the nut and tapered portion 1877 20 mL Sample Loop 2.0 mm (0.080”) ID x 1/8” OD
A of the ferrule toward the end. See Figure A. 7755-020 5 µL Sample Loop 0.18 mm (0.007”) ID x 1/16” OD
7755-021 10 µL Sample Loop 0.30 mm (0.012”) ID x 1/16” OD
b) Insert the tubing into port (4). Confirm that
the tubing is bottomed in the valve port as 7755-022 20 µL Sample Loop 0.30 mm (0.012”) ID x 1/16” OD
shown in Figure A. 7755-023 50 µL Sample Loop 0.51 mm (0.020”) ID x 1/16” OD
7755-024 100 µL Sample Loop 0.51 mm (0.020”) ID x 1/16” OD
c) While firmly pressing down on the tubing,
7755-025 200 µL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD
hand-tighten the nut as tight as possible.
7755-026 500 µL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD
B d) With the IDEX Wrench (see pages 33 and 140), 7755-027 1.0 mL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD
designed especially for fittings, tighten one 7755-028 2.0 mL Sample Loop 1.0 mm (0.040”) ID x 1/16” OD
quarter turn past finger tight. Remove the loop to 7755-029 5.0 mL Sample Loop 1.0 mm (0.040”) ID x 1/16” OD
confirm the ferrule is swaged onto the tube.
RHEODYNE STAINLESS STEEL LOOPS FOR 8125 INJECTOR
e) Repeat steps a-d with the other end of the (USE 7755-024 TO 7755-029 FOR VOLUMES > 50 µL)
loop while the swaged end remains outside 8020 5 µL Sample Loop 0.20 mm (0.008”) ID x 0.020” OD
the valve port. See Figure B. 8021 10 µL Sample Loop 0.20 mm (0.008”) ID x 0.020” OD
C 8022 20 µL Sample Loop 0.25 mm (0.010”) ID x 0.020” OD
f) Reinstall each end of the loop to their
respective ports. See Figure C. 8023 50 µL Sample Loop 0.30 mm (0.012”) ID x 0.020” OD
Figure 1 Cut-away view 8125-084 Ferrules for 0.020” (0.5 mm) Tubing
of stainless steel sample 8125-086 Ferrules for 0.020” (0.5 mm) Tubing, 4-pk
loop installation

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 Valves
Sample Loops

PEEK™ Sample Loops

Flexible PEEK sample loops are alternatives to stainless steel loops.
PEEK loop ends are provided with clean, straight cuts for easy valve
installation.

PEEK polymer is inert to almost all organic solvents and is

biocompatible, giving PEEK loops added versatility. Natural PEEK is
used for these sample loops. Like metal loops, the size designations
of PEEK loops are nominal. The actual volumes can differ from the
theoretical designations because of the ±0.05 mm (0.002”) tolerance
of the tubing bore. Accuracy of large PEEK loops (0.8 mm, 0.030” bore)
is about ±14%, intermediate loops (0.5 mm, 0.020”) ±21%, and small
loops (0.2 mm, 0.007”) ±65%.

PEEK loops are also supplied with unswaged RheFlex fittings but do
not require the same swaging precaution. The fittings can reposition
along the loop tubing when the fitting reinserts in the ports for correct
loop installation.

Please Note: Several of our PEEK Sample Loops can also be used with
Valco/VICI ® sample injectors. Please refer to the product lising on this
page to aid selection.

Valco/VICI-Compatible Stainless Steel Sample Loops Part No. Volume Tubing

PEEK LOOPS FOR 3725, 3725i INJECTORS
Valco No.

Valco-Compatible Stainless Steel Loops are manufactured by IDEX 3055-018 2.0 mL Sample Loop 1.6 mm (0.062”) ID x 1/8” OD N/A
Health & Science. These loops are designed for use with Valco valve 3055-019 5.0 mL Sample Loop 1.6 mm (0.062”) ID x 1/8” OD N/A
3055-023 10 mL Sample Loop 2.0 mm (0.080”) ID x 1/8” OD N/A
models CW6 and EC6W. Each loop has burr-free, polished ends and
3055-025 20 mL Sample Loop 2.0 mm (0.080”) ID x 1/8” OD N/A
is passivated and flushed with reagent-grade methanol to ensure
PEEK LOOPS FOR 9725, 9010, PR/EV750-100, PR/EV753-100 INJECTORS
cleanliness.
Part No. Volume Bore / Tubing Valco No.
9055-020 5.0 µL Sample Loop 0.18 mm (0.007”) ID x 1/16” OD SL5CWPK
Loops made with 1/16” OD tubing come complete with F-287
9055-021 10 µL Sample Loop 0.25 mm (0.010”) ID x 1/16” OD SL10WPK
SealTight™ Fittings, which are pressure rated to 9,000 psi (620 bar)1. 9055-022 20 µL Sample Loop 0.25 mm (0.010”) ID x 1/16” OD SL20WPK
The fittings and adapters that accompany the 1/8” OD sample loops 9055-023 50 µL Sample Loop 0.51 mm (0.020”) ID x 1/16” OD SL50WPK
are rated to 1,000 psi (69 bar)1. Volumes are stated at ±10%, with 9055-024 100 µL Sample Loop 0.51 mm (0.020”) ID x 1/16” OD SL100WPK
exact calibration services available. Each sample loop we calibrate is 9055-025 200 µL Sample Loop 0.51 mm (0.020”) ID x 1/16” OD N/A
documented and supplied with a calibration certificate. 9055-026 500 µL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD SL500WPK
9055-027 1.0 mL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD SL1KCWPK
9055-028 2.0 mL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD SL2KCWPK
9055-029 5.0 mL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD N/A
9055-033 10 mL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD N/A
PEEK LOOPS FOR 7725, 7725i, PR/EV700-100
7123-227 1 µL Sample Loop internal groove N/A
(model PR/EV700-100 only)
7755-015 2 µL Sample Loop internal groove N/A
(models 7725 and 7725i only)
REPLACEMENT RHEFLEX FITTINGS FOR PEEK LOOPS
Part No. Description Qty.
6000-078 Nut/Ferrule Set, Natural PEEK, 5/16-24, for 1/8” OD loops ea.
6000-079 Ferrules, Natural PEEK, for 1/8” OD loops 5-pk
1
These pressure ratings reflect the 6000-251 Ferrules, Natural PEEK, for 1/16” OD loops 10-pk
performance of the fittings, not the 6000-254 Nut/Ferrule Sets, Natural PEEK, 10-32, for 1/16” OD loops 10-pk
port or valve in which they are used.
VALCO/VICI-COMPATIBLE STAINLESS STEEL LOOPS FOR C6W, EC6W INJECTORS
IDEX Health & Science manufactures many Part No. Volume Tubing Valco No.
products designed as direct replacements for OEM
components. Reference to these manufacturers 1750 5 µL Sample Loop 0.18 mm (0.007”) ID x 1/16” OD SL5CW
does not imply their endorsement of our products. 1751 10 µL Sample Loop 0.25 mm (0.010”) ID x 1/16” OD SL10CW
1752 15 µL Sample Loop 0.25 mm (0.010”) ID x 1/16” OD SL15CW
1755 20 µL Sample Loop 0.51 mm (0.010”) ID x 1/16” OD SL20CW

Application Note 1758

1759
25 µL Sample Loop
50 µL Sample Loop
0.51 mm (0.010”) ID x 1/16” OD
0.51 mm (0.020”) ID x 1/16” OD
SL25CW
SL50CW
1762 100 µL Sample Loop 0.51 mm (0.020”) ID x 1/16” OD SL100CW
PEEK Physical Strength Characteristics 1778 200 µL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD N/A
Although PEEK material is compatible with virtually all solvents, there are 1763 250 µL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD SL250CW
many factors that affect burst pressure of PEEK tubing. Factors such as 1764 500 µL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD SL500CW
increases in inner diameter, temperature, exposure time, and concentration 1770 1 mL Sample Loop 0.76 mm (0.030”) ID x 1/16” OD SL1KCW
of organic solvents affect the degradation of PEEK. Other solvents such 1772 2 mL Sample Loop 1.02 mm (0.040”) ID x 1/16” OD SL2KCW
a THF, methylene chloride and DMSO cause PEEK tubing to swell while 1775 5 mL Sample Loop 2.03 mm (0.080”) ID x 1/8” OD SL5KCW
concentrated nitric acid and sulfuric acid weaken the tubing. 1776 10 mL Sample Loop 2.03 mm (0.080”) ID x 1/8” OD SL10KCW

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Valves
Application Notes

Application Note Application Note

Fluidic Movement in Tubes How to Find and Fix Common Sample Injector Leaks
Q: “
 Why can I load only up to half of the volume of the loop Leaks cause valuable sample loss. Nobody wants that. The key to the valve
in partial-filling method?” holding pressure is the integrity of the sealing surfaces. If there is a scratch
A: S
 ample occupies 2 µL of loop for every 1 µL loaded from the syringe. on the sealing surface, or the needle seal in the rotor seal is damaged, a
For example, 10 µL of sample spreads out over the entire length of a leak may appear. It is also important to realize what appears to be a leak
20 µL loop. Any additional sample loaded will overflow the end of the can instead be a result of siphoning. The following are the three most
loop and exit out to waste. Reproducibility is poor because the volume common situations in which fluid leaks occur.
of sample in the loop is different from the known volume originally 1. If fluid leaks out of the needle
loaded by your syringe. port only while loading the loop
(i.e., while pushing down on the
Sample plunger of the syringe), the problem
is most likely that the needle seal or
the needle port fitting in the loop filler
Mobile Phase port is not gripping the syringe needle
tightly enough. Tighten the needle
seal grip by pushing with the eraser
Tube Wall Flow end of the pencil on the needle port
Figure 1 Schematic of sample flow through mobile phase between tubing walls (See Figure 1). The tightening reduces
Figure 1 To reform the needle seal, the hole diameter of the needle seal
push the eraser end of a pencil and port fitting.
against the needle port
Fluid spreads in a parabolic shape through a tube instead of moving in one
plug because the velocity is different at the center of the tube than at the 2. If fluid leaks continuously from the needle port or vent lines and/or from
walls. The velocity at the center of the tube is twice the average velocity, the stator-to-stator ring interface, replace the rotor seal and/or stator face
and near the wall the velocity is almost zero, creating a parabolic shape. This assembly. Scratches on the rotor seal or cracks in the stator face assembly
fluidic movement is called laminar flow. See Figure 1. allow mobile phase to escape and cause cross port leakage. Genuine
Rheodyne replacement rotor seals are listed on page 134.
In dual mode injectors (see “Sample Loop Loading” Application Note on page 131)
the sample from the syringe needle loads directly into the sample loop. The sample 3. If fluid leaks from the needle port and/or vent lines but eventually stops,
volume is known since there is the cause is most likely siphoning and not a leak. Siphoning occurs if the
no sample waste. The laminar vent lines are lower or higher than the needle port. Adjust the vent line(s) so
flow phenomenon accounts for that the outlet is at the same horizontal level as the needle port to prevent
the shape of the plot as shown in siphoning. (See Figure 2).
Figure 2. Note that the plot has For other leakage or injection troubleshooting, refer to the Rheodyne
constant three regions: Troubleshooting Guide for HPLC Injection Problems. You may download the
Area of Peak

ar a) Partial-Filling Region. When Guide from the Rheodyne web site: www.idex-hs.com under Support.
ne
onli the volume dispensed is less than
n half the loop volume, the curve is
linear. Sample has not reached the
end of the loop. Within this region,
performance depends on the
linear

syringe and operator.

b) Nonlinear Region. When the
1 2 3 4 40 80 volume dispensed is between
Sample Dispensed (loop volumes) half the loop volume and about
two loop volumes, the curve is
Figure 2 Sample mass (observed peak area) nonlinear. Sample is lost from the
vs. volume of sample dispensed from the
syringe, in units of loop volumes, injected loop, so reproducibility is poor. If
onto the column from a Rheodyne® dual you dispense a volume equal to
mode injector such as model 7725 the loop size, you are in this region
of poor performance.
c) Complete-Filling Region. When the volume of sample dispensed is several loop
volumes, the loop contains only pure sample, undiluted by residual mobile phase.
Within this region, reproducibility is highest.
In the single mode injectors the sample must pass through a connecting passage Figure 2 Needle port level compared to the level of vent line outlet:
before it reaches the sample loop. Since some of the sample dispensed from the (A) siphoning occurs when the vent line outlet is above the needle port level
syringe remains in the connecting passageway, an unknown amount enters the (B) siphoning does not occur if the vent line outlet is the same horizontal level
sample loop. Therefore, single mode injectors achieve high reproducibility only by as the needle port
using the complete-filling method.

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 Valves
Adapters for Syringe Needles

Needle Port Accessories

The Rheodyne® adaptable Loop Filler Ports (Part #7012 and 9012) are
Application Note used to load sample from syringe needles or luer tips. The Needle Port
(Part #9013) conserves sample by minimizing the volume between the
Using Proper Syringe Needles needle and the valve.

rotor seal 9125-076

needle seal
7125-054
needle

A
Figure 1 A square cut needle:
(A) stops against the stator 7012 9012
needle port tube
face assembly; The tip of
stator face assembly a pointed needle
(B) slips into the stator face
rotor seal and the tip breaks off as the
needle seal valve rotates
needle 9013

B
Valve Adapter for 10-32 Ports
needle port tube
For 1/32” OD stainless steel tubing
stator face assembly Low swept volume
Extends the life of the rotor
With front-loading injectors it is important to use the correct needle when
loading the sample loop. An incorrect needle will damage the valve and As a result of customer requests, a Valve Adapter for 10-32 ports was
can cause poor reproducibility. When the needle is too short the tip will designed specifically for use with 1/32” OD stainless steel tubing.
not reach the needle seal. When the needle is too small in diameter the This product extends the life of and prevents damage to the rotor,
seal will not grip tightly enough. Needles with a beveled tip can damage guarding against such potential hazards M-400
the rotor seal and stator face assembly (see Figure 1). The needle should be as tubing that may pass through the
#22 gauge (0.028” – 0.0285”/ 0.72 mm), and 90° point style (square cut end). stator and scratch the rotor. This adapter
Model 3725 requires a #16 gauge (0.0645” – 0.0655”/ 1.65 mm) needle.
has a very low swept volume, at 300 nL.
NEVER USE A BEVELED, POINTED, OR TAPERED NEEDLE.
The all-PEEK ™ fluid pathway ensures
Needle specifications are not critical when using a Loop Filler Port to load biocompatibility.
the sample loop. However, it is important to tighten the needle port fitting
around the needle if using a syringe needle with a slightly smaller diameter
than 0.7 mm (0.028”).
If the loading method used is complete-filling, a syringe without a needle can
be used. A syringe fitted with a Needle Port Cleaner can be used with a front-
loading valve (Figure 2A) or with a Loop Filler Port (Figure 2B).
Needle port accessories are listed on this page. Tubing Seal P-416
Ferrule Assembly Micro Seal Nut

MicroFilter F-112 1/32” OD

Body Micro Tubing
Ferrule (not included)
M-400
Valve Adapter
(Includes indicated products)
Figure 2
(A) Syringe fitted with Needle Port Cleaner
(Part # 7125-054) loading a front-loading Part No. Description
valve (model 7725)
(B) loading a Loop Filler Port (Part # 7012) NEEDLE PORT ACCESSORIES
7012 Stainless Steel Loop Filler Port
7125-054 Needle Port Cleaner
9012 PEEK Loop Filler Port
B 9013 PEEK Needle Port
9125-076 Suction Needle Adapter (for Model 9725)
VALVE ADAPTER FOR 10-32 PORTS
F-112 Replacement MicroFerrule for M-400, Natural PEEK
M-400 Valve Adapter Assembly
P-416 Replacement Female Nut for M-400, Natural PEEK

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Valves
Valve Accessories

Micro Injection Port Adapters Medium Pressure Injection Port Adapter

 or 1/32” or 360 µm OD tubing
F This simple, biocompatible adapter is designed specifically for the
Mount on actuator, bracket or bulkhead Medium Pressure Injection Valves on page 142 and can also convert any
1/4-28 flat-bottom port into a port that can accept a standard 22 gauge
To introduce sample, connect 1/32” or 360 µm OD capillary tubing HPLC injection needle. This injection port adapter is adjustable, so you
to an Upchurch Scientific® Injection Port Adapter Assembly. These can create a snug fit around the needle to prevent any leaking of the
adapters accept standard 22 gauge Hamilton-style injection syringe analyte. In addition, this product features an internal stop that prevents
needles. No additional swept volume is added to the fluid pathway you from inserting the needle too far, eliminating the possibility of
by these adapters, as the needle butts damaging the valve with the needle tip.
directly against the connecting tubing V-447
during injections. The adapters can be
bulkhead mounted or mounted with the P-296
V-447 Kits. Refer to the chart below to Replaceable Tubing and Ferrule Assembly
(Included)
select the appropriate adapter assembly.

To introduce a sample directly into a 10-32

port, purchase a M-432-03 separately.

Tubing Adapter Body

(not included)
Needle Port
A B C P-295
Medium Pressure Injection Port Adapter

Mounting Brackets
M-432
Rheodyne® mounting brackets and panels of different shapes and
Micro Injection Port Adapter Assembly sizes organize and provide a sturdy support for Rheodyne valves.
The Ring Stand Mounting Bracket now allows the valves to mount onto
common laboratory equipment.

Replacement Parts
A B C 7160
For 1/32” OD Tubing 7160-010
M-433 P-416 F-112 M-432-03
For 360 µm OD Tubing
M-432 and V-447 P-416BLK F-152 M-432-03
*See diagram above
7160-029

IDEX Wrench
The smartly designed IDEX Wrench is a double-ended slotted socket Part No. Description
wrench that fits over 1/16” and 1/8” OD tubing. It easily loosens and MICRO INJECTION PORT ADAPTERS
tightens 1/4” and 5/16” hex head stainless steel or PEEK™ fittings. The For 1/32” OD Tubing
“Z” shape of the IDEX Wrench provides ideal leverage for changing F-112 Replacement MicroFerrule for M-433, Natural PEEK
sample loops and fittings, and keeps one end from restricting the use M-433 Micro Injection Port Adapter Assembly
of the other. M-432-03 Replacement Tubing/Fitting Assembly for M-432 & M-433
P-416 Replacement Female Nut for M-433, Natural PEEK
For 360 µm OD Tubing
F-152 Replacement MicroFerrule for M-432, Natural PEEK
M-432 Micro Injection Port Adapter Assembly
6810 M-432-03 Replacement Tubing/Fitting Assembly for M-432 & M-433
P-416BLK Replacement Female Nut for M-432, Black PEEK
V-447 Micro Injection Port Adapter Assembly Actuator Mounting Kit
Includes (1) M-432 with mini-actuator bracket and (2) mounting screws
IDEX WRENCH
6810 IDEX Wrench
MOUNTING BRACKET ACCESSORIES
7160 Mounting Panel
7160-010 Valve Angle Bracket
7160-029 Ring Stand Mounting Bracket
MEDIUM PRESSURE INJECTION PORT ADAPTER
P-295 Adjustable Injection Port Adapter
P-296 Replacement Tubing/Ferrule Assembly

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 Valves
Low Pressure Valves

MXX Low Pressure Valves

Switching and selection valve models 2 2
1 1
C
 an withstand up to 125 psi (9 bar)
Proprietary polymer combination allows interface for long life 3 3
All models accept 1/16” or 1/8” tubing (proper ferrules required) 6 6

4 4
5 5

Position 1 Position 2

Flow Path of MX Series II , Two-Position, Six-Port Switching Valve

™

1
2

6
3 7

5
4

Flow path of MX Series II Six-Position, Seven-Port Selector Valve

Specifications
Flow Passages 0.41 mm (0.016”) – 1.5 mm (0.060”) diameter
Power Requirements 100-120 VAC, 50-60 Hz
Regulatory Compliance CE Mark
Remote Control USB, I2C, BCD, Level Logic
Operating Temperature 0°– 40 °C, non-condensing
Storage Temperature 0°– 75 °C
Dimensions ( H x W x D) 117 mm x 76 mm x 128 mm (4.6” x 3.0” x 5.0”)

Part No. Description Pressure Rating Ferrule Size Tubing/Fittings Size Wetted Material
MXX LOW PRESSURE VALVES
MXX777-601 2-position, 6-port, Switching Valve 125 psi (8.5 bar) 1/16” Accepts Either 1/16” or 1/8” Tubing RPC-7*
MXX777-603 2-position, Double Three Way 125 psi (8.5 bar) 1/16” Accepts Either 1/16” or 1/8” Tubing RPC-7
MXX777-605 6-position, 7-port, Selection Valve 125 psi (8.5 bar) 1/16” Accepts Either 1/16” or 1/8” Tubing RPC-7
MXX777-612 2-Position, 6-Port, Large Bore Switching Valve 125 psi (8.5 bar) 1/8” Accepts Either 1/16” or 1/8” Tubing RPC-7
MXX777-616 6-position, 7-port, Large Bore Selection Valve 125 psi (8.5 bar) 1/8” Accepts Either 1/16” or 1/8” Tubing RPC-7
MXX778-605 10-position, 11-port, Selection Valve 125 psi (8.5 bar) 1/16” Accepts Either 1/16” or 1/8” Tubing RPC-7
REPLACEMENT FITTINGS
7770-039 Ferrules for 1/8” OD Tubing — — — 25-pk
7770-040 Ferrules for 1/8” Tubing — — — 50-pk
7770-041 Ferrules for 1/8” Tubing — — — 100-pk
7770-044 Ferrules for 1/16” OD Tubing — — — 25-pk
7770-045 Ferrules for 1/16” Tubing — — — 50-pk
7770-046 Ferrules for 1/16” Tubing — — — 100-pk
7770-124 O-rings for 1/16” OD Tubing — — — 25-pk
* RPC-7 Proprietary Polymer Combination

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Valves
Medium Pressure Injection Valves

Medium Pressure Injection Valves

 iocompatible and inert
B
Low swept volume
Pressure rated to 500 psi (34 bar), ceramic version and
1,000 psi (69 bar), polymer version
The Upchurch Scientific® 6-Port Medium Pressure Injection Valves
feature easy, external sample loop customization. Simply clean-cut a
piece of tubing that represents the volume of sample you wish to inject,
taking into account the port-to-port volume of the valve. Please refer
to the “Tubing Internal Diameters and Volumes” chart on page 198
for assistance in calculating the volume of a length of tubing.

Two biocompatible injection valve options are available:

Long-Life Ceramic
V-1461-DC
Featuring ceramic-to-ceramic sealing surfaces, these long-life valves V-1541-DC
have been tested to over one million actuations with no change in
performance.* The wetted materials are chemically resistant ceramic
and PEEK™ polymer and the valves are rated to 500 psi (34 bar). Each
valve includes 1/4-28 Flangeless Fittings for 1/16” OD tubing. V-451

Economical Polymer
These economical valves have chemically resistant Vespel® and Specifications
PTFE resin wetted surfaces and are rated for a maximum pressure of
Dimensions (L x W), Port to Port
1,000 psi (69 bar). Each valve includes 1/4-28 Flangeless Fittings for Valve Part No. Thru-Hole excluding fittings Volume
1/16” or 1/8” OD tubing. M-460 0.040" (1.0 mm) 2.75” x 1.30” (69.8 x 33.0 mm) 16.9 µL
M-461 0.040" (1.0 mm) 3.00” x 1.30” (76.2 x 33.0 mm) 16.9 µL
Both valve options are available in three configurations: V-450 0.040" (1.0 mm) 2.72” x 1.24”1 (69.1 x 31.5 mm) 5.1 µL
standard manual, bulkhead mountable manual and actuated. V-540 0.063" (1.6 mm) 2.72” x 1.24”1 (69.1 x 31.5 mm) 12.7 µL
Valves can be premounted on a DC gear motor actuator to V-451 0.040" (1.0 mm) 2.92” x 1.24”1 (74.2 x 31.5 mm) 5.1 µL
automate valve positioning. Each actuated valve is provided V-541 0.063" (1.6 mm) 2.92” x 1.24”1 (74.2 x 31.5 mm) 12.7 µL
with an inline power adapter which allows operation from 1
Width of the knob, which is slightly wider than the valve body
110/120V and 220/240V AC power, a RS-232 and contact closure
communication cable and a cable for multiplexing valves in series. Viewpoint from Rotary Knob
* Tested using room-temperature water as the solvent
1 Sample In 1 Sample In
(Needle Port) Pump (Needle Port)
Pump
6 2 6 2

Sample
1.21” Waste
(3.07 cm) Sample
5 3 5 3 Waste
Standard Version 2.72” (6.90 cm) Column Column
4 4

Bulkhead Version 2.92” (7.42 cm) Position A (LOAD) Position B (INJECT)

Flow Path of Medium Pressure Injection Valve

(highlighting flow through sample loop in each position)

Wetted Tubing
Part No. Description Material OD Includes
MEDIUM PRESSURE INJECTION VALVES, MANUAL
M-460 Injection Valve Ceramic 1/16” (6) XP-235
M-461 Bulkhead version of M-460 Ceramic 1/16” (6) XP-235
V-540
V-104 5/64” Allen Wrench (replacement)
Medium Pressure Injection Valve
V-450 Injection Valve Polymer 1/16” (6) XP-235
V-451 Bulkhead version of V-450 Polymer 1/16” (6) XP-235
V-540 Injection Valve Polymer 1/8” (6) XP-335
V-541 Bulkhead version of V-540 Polymer 1/8” (6) XP-335
MEDIUM PRESSURE INJECTION VALVES, ACTUATED
V-1451-DC Actuated version of V-451, DC Gear Motor (premounted)
Top Seller SEE STARRED PRODUCTS V-1461-DC Actuated version of M-461, DC Gear Motor (premounted)
V-1541-DC Actuated version of V-541, DC Gear Motor (premounted)

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 Valves
Medium Pressure Selection Valves

Medium Pressure 6-Way Selection Valves

 iocompatible and inert
B
Low swept volume
Excellent choice for mobile phase selection
The Upchurch Scientific® 6-Way Medium Pressure Selection Valves have
a common inlet (or outlet) port connected to six different ports.

Two biocompatible selection valve options are available. For more

information on the Long-Life Ceramic and Economical Polymer valves,
please see the previous page.

If your application does not require all six outer ports, seal the extra
port(s) with any flat-bottom 1/4-28 plug from page 32.

Both valve options are available in three configurations: standard manual,

bulkhead mountable manual and actuated. A valve mounting bracket
(M-615-2) is listed on page 144. Valves can be premounted on a DC V-1471-DC
gear motor actuator to automate valve positioning. Each actuated V-1241-DC
valve is provided with an inline power adapter which allows operation
from 110/120V and 220/240V AC power, a RS-232 and contact closure
communication cable and a cable for multiplexing valves in series. V-340

Application Note Specifications

Use our 6-Way Medium Pressure Selection Valve to easily select different Dimensions (L x W), Port to Port
solvents with an isocratic pump. The setup below involves an isocratic pump Valve Part No. Thru-Hole excluding fittings Volume
used for two separate assays, each requiring a pre-rinsing step prior to use. M-470 0.040” (1.0 mm) 2.75” x 1.30” (69.8 x 33.0 mm) 16.1 µL
Using our Selection Valve in this setup, the system can be primed with the M-471 0.040” (1.0 mm) 3.00” x 1.30” (76.2 x 33.0 mm) 16.1 µL
solvent in the first reservoir. Then, solvent from the second reservoir can be V-240 0.040" (1.0 mm) 2.72” x 1.24”1 (69.1 x 31.5 mm) 16.1 µL
used as the mobile phase for the first application. Later, the system can be V-241 0.040” (1.0 mm) 2.72” x 1.24”1 (69.1 x 31.5 mm) 16.1 µL
rinsed with an intermediate buffer solution from the third reservoir, followed V-340 0.063” (1.6 mm) 2.92” x 1.24”1 (74.2 x 31.5 mm) 18.1 µL
by switching over to the second mobile phase in reservoir number 4. At the V-341 0.063” (1.6 mm) 2.92” x 1.24”1 (74.2 x 31.5 mm) 18.1 µL
end of the day, the system can be rinsed with water from a fifth reservoir to
1
Width of the knob, which is slightly wider than the valve body
avoid the possibility of precipitation on wetted surfaces. Follow this rinse with
a final rinse of methanol from the sixth reservoir for system storage.

#2 6-Way Valve
Mobile #1
Phase 1 Priming
Solution

#6
#3
Intermediate
MeOH Top Seller SEE STARRED PRODUCTS
Buffer
Solution #5
Rinse
Water Wetted Tubing
Part No. Description Material OD Includes
#4
Mobile MEDIUM PRESSURE 6-WAY SELECTION VALVES, MANUAL
Phase 2 M-470 6-Way Selection Valve Ceramic 1/16” (6) XP-235
Column
M-471 Bulkhead version of M-470 Ceramic 1/16” (6) XP-235
Sample Injection V-104 5/64” Allen Wrench (replacement)
Isocratic Pump Valve V-240 6-Way Selection Valve Polymer 1/16” (6) XP-235
V-241 Bulkhead version of V-240 Polymer 1/16” (6) XP-235
V-340 6-Way Selection Valve Polymer 1/8” (6) XP-335
V-341 Bulkhead version of V-340 Polymer 1/8” (6) XP-335
Waste MEDIUM PRESSURE 6-WAY SELECTION VALVES, ACTUATED
V-1241-DC Actuated version of V-241, DC Gear (premounted)
Data Recording Detector V-1341-DC Actuated version of V-341, DC Gear (premounted)
Device
V-1471-DC Actuated version of V-471, DC Gear (premounted)

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Valves
Medium Pressure Switching Valves

Medium Pressure 4-Port Switching Valves

 hree flow configurations to choose from
T
Biocompatible flow paths, PEEK™ and PTFE
Pressure rated to 500 psi (34 bar)
With three versions to choose from, Upchurch Scientific® Switching Valves
offer several flow distribution options. Each V-100 Series Switching Valve
has 0.040” (1.0 mm) thru-holes and is supplied with Flangeless Fittings
for use with 1/16” OD tubing.

Each 4-Port Switching Valve is available in standard manual and

bulkhead mountable manual configurations. The bulkhead mountable
manual configuration can be mounted on the M-615-1 Valve Bracket
(see below). DC gear motor actuated versions are also available for
automated valve positioning. Each actuated valve is provided with
an inline power adapter which allows operation from 110/120V and
220/240V AC power, a RS-232 and contact closure communication V-101L
cable and a cable for multiplexing valves in series.
V-1101D-DC

Valve Mounting Brackets

Mount the bulkhead mountable manual valves with these Brackets.
Once mounted, a valve will be positioned horizontally. Extra holes are 1.80” 2.18”
(4.57 cm) (5.54 cm)
provided for valve accessories.

Made of sturdy anodized aluminum, these brackets can be attached

to almost any lab surface. Each bracket comes with the required valve
mounting hardware and instructions.

0.66”
(1.68 cm) 0.66”
(1.68 cm)
V-100 V-101
Standard Version Bulkhead-Mount Version

3.4”
(8.64 cm)
Related Products
To use these valves with 1/8” OD tubing, simply replace the supplied
fittings with 1/4-28 Flangeless Fittings found on pages 24 – 27. Please Note:
To operate, all ports must be either connected to a line of your system or
2.0” plugged. Comes complete with P-218BLK/P-240 Flangeless Fittings. “L”
M-615-2 (5.08 cm) and “T” valve versions also include a P-309 Plug.
Valve Bracket,
shown with an M-461 2.9”
Injection Valve (not included) (7.37 cm)
Top Seller SEE STARRED PRODUCTS

Part No. Description Swept Volume

MEDIUM PRESSURE 4-PORT SWITCHING VALVES, MANUAL
V-100D 4-Way Diagonal Flow Switching Valve 12.4 µL
Switching Valve Options V-100L Right Angle Flow Switching Valve 6.2 µL
V-100T 3-Way Flow Switching Valve 9.7 µL
1 1 1 V-101D Bulkhead Version of V-100D 12.4 µL
V-101L Bulkhead Version of V-100L 6.2 µL
V-101T Bulkhead Version of V-100T 9.7 µL
4 4 4 V-103 0.050” Allen Wrench (replacement)
2 2 2 MEDIUM PRESSURE 4-PORT SWITCHING VALVES, ACTUATED
V-1101D-DC Actuated Version of V-101D 12.4 µL
3 3 3 V-1101L-DC Actuated Version of V-101L 6.2 µL
V-1101T-DC Actuated Version of V-101T 9.7 µL
4-Way Diagonal Flow Right Angle Flow 3-Way Flow VALVE BRACKET
Switching Valves Switching Valves Switching Valves
M-615-1 Mounting Bracket for Switching Valves (page 144)
(V-100D, V-101D, (V-100L, V-101L, (V-100T, V-101T,
V-1101D-DC) V-1101L-DC) V-1101T-DC) M-615-2 Mounting Bracket for Injection and Selection Valves (this page)

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 Valves
Switching and Shut-Off Valves

Shut-Off Valves
For low and medium pressure applications
Application Note Biocompatible, all-polymer flow path
Available for 1/16” and 1/8” OD tubing
Medium Pressure Switching Valve Applications
Pressure rated to 500 psi (34 bar)
Protect sensitive system components (such as a column) during a cleaning
cycle with our Diagonal Flow Switching Valve (“D”). This valve eliminates the Stop a flow stream quickly with Upchurch Scientific® biocompatible
need to remove, plug and reconnect a low pressure column (see below). Shut-Off Valves. The bodies are manufactured from either PEEK™
or ETFE, and both versions feature a PCTFE rotor, making them
Pump Pump highly resistant to chemical attack. The blue colorant used in some
valve configurations has proven not to leach out with common
HPLC solvents.
Detector Detector
Connect semi-rigid or rigid tubing, such as PEEK, stainless steel or
fluoropolymer, with the 1/4-28 Flangeless Fittings provided. Soft
Co

Co

tubing, such as PharMed® or Tygon® (see pages 75 – 89), may be

lum

lum
n

connected to these valves using our 1/4-28 barbed adapters, found

Position 1 Position 2 on page 58.

A typical application for a Right Angle Flow Switching Valve (“L”) is column
switching, allowing two columns to use one detector. Detector switching
is another common application for this valve (see below). Plug off the extra
port with the included plug.

Column Column

V-100L Valve V-100L Valve 1.00”

(2.54 cm)

Detector Detector Detector Detector

#1 #2 #1 #2

Plug Plug
Position 1 Position 2

0.145”
In (0.37 cm)
 our detector switching
Y diameter
application may require 1.07” 0.50”
the flexibility of routing the Detector #1 Detector #2 (2.72 cm) (1.27 cm)
column effluent to both
detectors simultaneously
while retaining the ability to
Position 1
isolate each detector. Use our P-733 P-783
3-Way Flow Switching Valve In PEEK Shut-Off Valve ETFE Shut-Off Valve
(“T”), plugging off the fourth
port with the included plug
Detector #1 Detector #2
(see right).

Position 2
Application Note
In Install a Shut-Off Valve between the solvent reservoir and pump. Then, when
it is necessary to disconnect the solvent line, a simple twist of the valve lever
eliminates the potential of a hazardous spill.
Detector #1 Detector #2

OD Internal
Part No. Material Tubing Thru-hole Volume* Includes
Position 3
SHUT-OFF VALVES
P-721 ETFE, Natural 1/8” 0.040” (1.0 mm) 10.0 µL (2) P-335, (2) P-300N
P-732 PEEK, Natural 1/16” 0.020” (0.5 mm) 2.5 µL (2) XP-235
P-733 PEEK, Natural 1/8” 0.040” (1.0 mm) 10.0 µL (2) XP-335
P-782 ETFE, Blue 1/16” 0.020” (0.5 mm) 2.5 µL (2) XP-235

Top Seller SEE STARRED PRODUCTS P-783 ETFE, Blue 1/8” 0.040” (1.0 mm)
* Maximum internal volume, with valve fully open
10.0 µL (2) XP-335

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Valves
Micro-Splitter Valves

Micro-Splitter Valves
 or interfacing LC-MS systems
F
Adjustable split stream flow rates
Medium and high pressure versions
The Upchurch Scientific® line of Micro-Splitter Valves is designed to
accurately split and control a low-flow stream off a single incoming supply.
P-470
Choose between 1/4-28 flat-bottom and 10-32 coned threaded
versions. A valve is also available to accommodate applications
involving capillary tubing with a 6-32 coned threaded fitting for
MicroTight® tubing sleeves (see page 19).

The High Pressure Micro-Splitter Valves are designed to operate M-405S

successfully up to 4,000 psi (276 bar) and the medium pressure Micro-
Splitter valves are pressure rated to 800 psi (55 bar).

Graduated Valves offer many of the benefits and features of Micro-

Splitter Valves, plus the ability to adjust and set the split flow to
P-450
repeatable settings. This allows documentation of settings and the
resulting flow rates for easier method development. The graduations
also make it easier to employ the valve in a system used to run multiple
analyses that require different split flow rates. Choose from regular
High Pressure and Capillary versions.

P-451
Thumbscrew Medium Pressure
Micro-Splitter Valve

1.40”
(3.56 cm)

Application Note
With an incoming flow rate of 1mL/min using room temperature water
and equal pressures on both outlet lines, the minimum split flow rate
1.13” is 2 µL/min for the medium pressure micro-splitter valves and 4.8 µL/min
(2.87 cm)
for the high pressure micro-splitter valves.
All Micro-Splitter Valves have been tested at flow rates to 100 mL/min,
with a maximum resulting pressure drop of only 45 psi (3.1 bar) when
the valve is fully opened.

Top Seller SEE STARRED PRODUCTS

Specifications
Part No. Description Includes
Internal Volume1 Max. Operating
Part No. Valve Type Threads (closed/fully open) Pressure MICRO-SPLITTER VALVES
M-405S ,T High Pres. Capillary 10-32 / 6-32 1.2 / 2.4 µL 4,000 psi (276 bar) P-450 Medium Pressure, 1/4-28, Biocompatible (3) XP-235
M-472 High Pres. Cap. Grad. 10-32 / 6-32 1.2 / 2.4 µL 4,000 psi (276 bar) P-451 Medium Pressure, 10-32, Biocompatible (3) F-120
P-460S High Pressure, 10-32, with Stainless Steel Needle (3) F-120
P-450 Medium Pressure 1/4-28 2.1 / 4.1 µL 800 psi (55 bar)
P-460T High Pressure, 10-32, with Titanium Needle (3) F-120
P-451 Medium Pressure 10-32 1.2 / 2.8 µL 800 psi (55 bar)
GRADUATED MICRO-SPLITTER VALVES
P-452 Medium Pres. Capillary 10-32 / 6-32 1.2 / 2.4 µL 800 psi (55 bar)
M-472 High Pressure Capillary Graduated, 10-32/6-32, (2) F-120, (1) F-125*
P-460S, T High Pressure 10-32 1.2 / 2.8 µL 4,000 psi (276 bar) with Stainless Steel Needle
P-470 High Pres. Graduated 10-32 1.2 / 2.8 µL 4,000 psi (276 bar) P-470 High Pressure Graduated, 10-32, (3) F-120
The supply and waste port thru-holes have IDs of 0.020” (0.50 mm). The ID for the split-stream port
1  with Stainless Steel Needle
thru-hole is 0.020” (0.50 mm) in standard versions; in capillary versions it is 0.010” (0.25 mm). * Use with the MicroTight Tubing Sleeves, found on page 19

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 Valves
Micro-Metering Valves

Micro-Metering Valves
Flow rates as low as 3.5 µL/min*
Application Note 1/4-28 flat-bottom and 10-32 coned designs available
Materials of construction: PEEK™, PTFE
Back Pressure Considerations
The Micro-Splitter Valves are designed to work when both effluent flow path
For fine control of fluid flow rates, Micro-Metering Valves can reduce
pressures are nearly identical. However, the split flow path will often have outgoing flow to as low as 3.5 µL/min*. These needle valves are
higher back pressure than the waste flow path, making it hard to achieve perfect for use with peristaltic pump fluid-transfer applications, mass
any split flow at all. There are two possible solutions. Place a back pressure spectrometry, and fraction collection.
regulator (see pages 152 –153) on the waste flow path that is equal to or
slightly greater than the pressure on the split flow path. Or, switch the two Upchurch Scientific® Micro-Metering Valves can also be used to
effluent pathways such that the split flow pathway is attached to the “waste” regulate gas flow in helium sparging lines and as a flow-dependent
port on the valve and the waste flow pathway is attached to the “split” variable back pressure regulator. For flow independent regulation of
port on the valve. (Please Note: This second method may result in a loss of back pressure, please see pages 152 –153.
adjustment sensitivity.)
Flow path materials are PEEK polymer and PTFE. All versions of this
Prime/Purge Valve valve have 0.020” (0.50 mm) thru-holes.
Air within the pump head can cause noisy pump operation and flow
instability. Solve this problem by placing a High Pressure Micro-Splitter Valve *At 1.0 mL/min incoming flow rate with room temperature water
(page 146) inline between the pump and the injector valve. You can then
safely divert pump flow to a waste container at a sufficient rate to dislodge
the air. Remove air from the solvent line leading to the pump with a Low
Pressure Prime/Purge Valve (page 155).

Multi-Column and Detector Systems

Does your work require analyses with multiple columns and detectors that use
the same mobile phase? If so, install one of our High Pressure Micro-Splitter
Valves after your injector. A single injection can then be split to two separate
columns and detector systems, at two different flow rates. This economical
set-up eliminates the need for an additional pump and injector valve, while
allowing data to be obtained simultaneously.
OUT
Post-Detector Interfacing 0.73” IN
(1.85 cm)
Use a Medium Pressure Micro-Splitter Valve to route fluid exiting an initial 0.49”
detector to other devices, such as a mass spectrometer and a fraction (1.24 cm)
collector. The valve will split and reduce the flow rate to that required for MS
interfacing, while diverting the remainder of the flow to the collector (a back
pressure regulator may also be required for this set up, available on pages
152 – 153).

Other Applications
These valves are also suited for other applications, such as adapting a
Thumbscrew
standard HPLC system to handle microbore analyses. For more information
1.28”
and plumbing diagrams for this application and those listed above, please (3.25 cm)
contact your local distributor or IDEX Health & Science directly.

P-445
1/4-28 threaded
PEEK Micro-Metering Valve

Top Seller SEE STARRED PRODUCTS

Internal
Part No. Material OD Tubing Thru-hole Volume* Includes
MICRO-METERING VALVES
P-445 PEEK, Black 1/16” 0.020” (0.50 mm) 7.7 µL (2) XP-230
P-446 PEEK, Black 1/16” 0.020” (0.50 mm) 7.2 µL (2) F-120

Order Online WWW. IDEX-HS .COM

P-447
*
PEEK, Black 1/8” 0.020” (0.50 mm) 7.7 µL
Maximum internal volume, with valve fully open
(2) XP-330

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