AS 2 BIOMOLECULES Do not
write in
QUESTIONSHEET 1 margin
(a) Show how two molecules of glucose below join together to form a disaccharide.
C O H H C O H
H
C C + C C
HO C C OH HO C C OH
[2]
(b) What is the name given to this type of reaction?
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[1]
(c) What is the name of the reverse reaction?
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(d) Glycogen is a complex storage polysaccharide. In which organ is it mainly stored in vertebrates?
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[1]
(e) A solution which tested negative for reducing sugars was thought to contain sucrose and protein. Describe
how you would find out whether this was so.
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[6]
TOTAL / 11
AS 2 BIOMOLECULES Do not
write in
QUESTIONSHEET 2 margin
The diagrams below show two different forms of glucose
α−
α−glucose β−
β−glucose
CH2OH CH2OH
H C O H H C O OH
C OH H C C OH H C
HO C C OH HO C C H
H OH H OH
(a) What term is used to describe these two forms?
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[1]
(b) How do the two forms of glucose differ from one another?
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(c) Why is it important to have these two differing forms?
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(d) What kinds of bonds occur in carbohydrates?
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[1]
TOTAL / 5
AS 2 BIOMOLECULES Do not
write in
QUESTIONSHEET 6 margin
Proteins can exist as primary, secondary, tertiary or quaternary structures.
The diagram below shows one of these protein structures.
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polypeptide chains
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H-bonds
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(a) What is the name given to this structure?
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[1]
(b) What gives this protein structure its high tensile strength?
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(c) What is meant by tertiary protein structure?
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[3]
(d) Name two types of bond which hold tertiary protein structure together.
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[2]
(e) Name one common protein which has a quaternary protein structure.
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[1]
(f) Describe how you would carry out a test to detect the presence of protein in an unknown sample solution.
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[3]
TOTAL / 11
AS 2 BIOMOLECULES Do not
write in
QUESTIONSHEET 19 margin
(a) Complete the table below by filling in the name of the missing molecules.
Macromolecule Composition
....................................................................... sugar/base/phosphate
Proteins .......................................................................
Fats fatty acids ................................................
Glycogen .......................................................................
Cellulose .......................................................................
[5]
(b) What factors determine the shape (conformation ) of a protein?
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[2]
TOTAL / 7
AS 3 ENZYMES Do not
write in
QUESTIONSHEET 3 margin
The graph shows the effect of substrate concentration on the rate of an enzyme controlled reaction.
c d
Rate
rate
a
substrate
Substrateconcentration
concentration
(a) Explain the shape of the graph between:
(i) a and b.
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[3]
(ii) c and d.
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[3]
The figure shows the effect of pH on the rate of an enzyme controlled reaction.
rate Rate
low pH high
(b) Explain the shape of the curve.
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[4]
TOTAL / 10
AS 3 ENZYMES Do not
write in
QUESTIONSHEET 5 margin
The graph shows the effect of substrate concentration on the rate of an enzyme-controlled reaction with and
without the addition of a competitive inhibitor.
Without inhibitor
of reaction
With inhibitor
reaction
Rate
rate of
Substrateconcentration
substrate concentration
(a) Define the term ‘competitive inhibitor’.
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[2]
(b) Suggest an explanation for the effect of the inhibitor on the rate of reaction when substrate concentration is
high.
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[2]
(c) State three differences between competitive and non-competitive inhibitors.
1: ........................................................................................................................................................................
2: ........................................................................................................................................................................
3: ........................................................................................................................................................................
[3]
TOTAL / 7
AS 3 ENZYMES Do not
write in
QUESTIONSHEET 9 margin
The diagram shows the effect of an allosteric inhibitor on an enzyme.
normal substrate
active site
allosteric inhibitor about to bind to
enzyme
(a) What is the effect of the allosteric inhibitor on the enzyme?
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[1]
(b) (i) What is the effect of the allosteric inhibitor on the catalytic activity of the enzyme.
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[1]
(ii) Explain why this occurs.
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[2]
Substances X and Y, shown below, are inhibitors of glucose phosphorylase.
COO- CH2OH
O
N
OO C S
N
H (CH2)3CH3
X Y
(c) Which substance is likely to be a competitive inhibitor of glucose phosphorylase?
Explain your answer.
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[3]
TOTAL / 7
AS 3 ENZYMES Do not
write in
QUESTIONSHEET 12 margin
The graphs below show the effect of a number of factors on the rate of enzyme controlled reactions.
Rate
Rate
A B
Rate
Rate
C D
(a) Which graph shows the most likely effect of changing:
(i) substrate concentration?
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[1]
(ii) temperature?
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[1]
(iii) pH?
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(iv) enzyme concentration when substrate is unlimited?
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(b) Name a competitive inhibitor and the enzyme it inhibits.
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[2]
TOTAL / 6
