The Creative Approach to Bioscience
Sodium
Single Reagent
REF: 303 001 (2 x 25 ml) 50 test
REF: 303 002 (4 x 25 ml) 100 test
REF: 303 003 (2 x 100 ml) 200 test
REF: 303 004 (2 x 500 ml) 1000 test
Intended Use System Parameters
Spectrum-Diagnostics Sodium reagent is intended for the in-vitro Wavelength 630 nm
quantitative diagnostic estimation of sodium in human serum on Optical path 1 cm
manual systems. Assay type colorimetric end-point
Direction Increase
Background Sample: Reagent Ratio 1:100
Temperature Room temperature
Sodium and Potassium are the major cations of extracellular and Zero adjustment Against reagent blank
intracellular fluids respectively. Sodium maintains the normal Sensitivity 55 mEq/l.
distribution of water and the osmotic pressure in the various fluid Linearity 180 mEq/l
compartments. Potassium influences the acid base balance and Incubation 5 min.
osmotic pressure including water retention. Increased sodium levels Blank absorbance limit 1.2
are found in severe dehydration and excessive treatment with sodium
salts. Decreased levels are found in severe polyurea, metabolic Procedure
acidosis, diarrhoea and renal insufficiency. Increased potassium
levels are found in renal failure, dehydration, shock and adrenal Pipette into clean test tubes:
insufficiency. Decreased levels are found in malnutrition,
gastrointestinal fluid loss and hyperactivity of the adrenal cortex. Blank Standard Sample
Method Reagent (R) 1 ml 1 ml 1 ml
Colorimetric method. Standard ......... 10 l .........
Assay Principle Sample ......... ......... 10 l
The Present method is based on reaction of sodium with a selective Mix well and let stand for 5 minutes at Room Temperature.
chromogen producing a chromophore whose absorbance varies Read absorbances ,A standard and A sample against Reagent Blank
directly as the concentration of sodium in the test specimen. at 630 nm.
Reagents Calculation
Sample
Reagent (R) Color Reagent Serum Sodium Conc.(mEq/l) = x 150
Standard
Chromogen 0.03 gm/L
EDTA 25 mmol/L Quality Control
Dimethyl sulfoxide (DMSO) 75 mmol/L
Preservatives 0.05 % Normal and abnormal control serum of known concentrations should
Antifoam 0.01 % be analyzed with each run.
Standard (S) Sodium 150 mEq/l Performance Characterstics
Precautions and Warnings Precision
Within run (Repeatability)
Do not ingest or inhalate. In case of contact with eyes or skin; rinse
immediately with plenty of soap and water. In case of severe injuries; Level 1 Level 2
seek medical advice immediately.
n 20 20
Reagent Storage and Stability Mean (mEq/l) 140 170
Reagents and standard are ready-to-use. When stored at 15 - 25oC; SD 0.72 1.44
they are stable up to the expiry date stated on the label.
Once opened, the reagent and standard are stable for 3 months at CV% 0.51 0.84
the specified temperature.
Run to run (Reproducibility)
Deterioration
Level 1 Level 2
Failure to recover control values within the assigned range may be n 20 20
an indication of reagent deterioration.
Mean (mEq/l) 140 170
Sample Preparation and Preservation SD 0.76 1.58
Serum and plasma CV% 0.54 0.93
Freshly drawn non-hemolysed serum is the specimen of choice.
Heparinised plasma can also be used.
Stability: Serum Sodium is stable for at least 24 hours at room Methods Comparison
temperature and two weeks at 2-8°C.
A comparison between Spectrum Diagnostics Sodium reagent and
Urine a commercial reagent of the same methodology was performed on
Urine diluted 1+1 with distilled water can be used for Sodium 20 human sera. A correlation of 0.979 was obtained.
estimation.
Sensitivity
ORDERING INFORMATION
When run as recommended, the minimum detection limit of the
assay 55 mEq/l.
CATALOG NO. QUANTITY
Linearity 303 001 2 x 25 ml 50 Test
303 002 4 x 25 ml 100 Test
The assay is linear up to 180 mEq/l.
303 003 2 x100 ml 200 Test
303 004 2 x500 ml 1000 Test
Interfering Substances
Hemoglobin and Lithium
Demonstrates positive interference
Lipemia
No significant interference
Other Ions
No adverse influence is exerted on the procedure by blood calcium,
chloride and potassium levels of up to 3 times normal values.
Hypermagnesemia may interfere with sodium assay.
Anticoagulants
Complexing Anticoagulants such as citrate and oxalate must be
avoided.
Expected Values
Serum : 135 – 150 mEq/l.
Urine(24 hr): 40-220 mEq/ 24 hr
Note:
It is recommended for each laboratory to establish and maintain its
own reference values. The given data are only an indication.
Spectrum Diagnostics does not interpret the results of a clinical
laboratory procedure; interpretation of the results is considered
the responsibility of qualified medical personnel. All indications
of clinical significance are supported by literature references.
Analytical Range
55 – 180 mEq/l.
Waste Disposal
This product is made to be used in professional laboratories.
Please consult local regulations for a correct waste disposal.
S56: dispose of this material and its container at hazardous or
special waste collection point.
S57: use appropriate container to avoid environmental contamination.
S61: avoid release in environment. refer to special instructions/safety
data sheets.
References
1. Tietz, N.W., Fundamentals of clinical Chemistry, W.b. Saunders
Co. Phila, P.A. p. 874.
2. Henry R.F., et, al, Clinical Chemistry Principles and Technics. 2nd
Ed, Harper and Row, Harper and Row, Hargersein, M.D. (1974)
3. Maruna RFL., Clin Chem. Acta. 2:581, (1958)
4. Trinder, P:Analyst, 76:596, (1951)
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