SHRI ATAL BIHARI VAJPAYEE MEDICAL COLLEGE & RESEARCH INSTITUTE

DEPARTMENT OF BIOCHEMISTRY

Practicals for Phase-I MBBS

2023
SHRI ATAL BIHARI VAJPAYEE MEDICAL COLLEGE & RESEARCH INSTITUTE

DEPARTMENT OF BIOCHEMISTRY
MBBS Practical Examination - 2023

Analysis of Glucose
1 Performance
Normal 1 Major Case
Station Theory,
Constituents of Urea Application &
Urine or 1 Minor Case Clinical Based
Abnormal
3 Response
Constituents of Total Protein
Stations
Urine

Creatinine
SHRI ATAL BIHARI VAJPAYEE MEDICAL COLLEGE & RESEARCH INSTITUTE

Exercise – 1: OSPE (20 Marks)

Number of Stations: 4

Duration for each Station: 5 Minutes

5 marks for each stations

3 Response Stations in Demo Room

1 Performance Station in Practical Hall
SHRI ATAL BIHARI VAJPAYEE MEDICAL COLLEGE & RESEARCH INSTITUTE

Exercise – 2: Qualitative Experiment (20 Marks)

Perform the tests

Record in the form of Procedure, Observation, Inference
Summarise the experiment in the form of Results &
interpretation
SHRI ATAL BIHARI VAJPAYEE MEDICAL COLLEGE & RESEARCH INSTITUTE

Exercise – 3: Quantitative Experiment (20 Marks)

write the Principle & Procedure of given quantitative

experiment in a separate booklet
Calculate and Report the results by using the formula and
substituting the obtained OD values
SHRI ATAL BIHARI VAJPAYEE MEDICAL COLLEGE & RESEARCH INSTITUTE

Exercise – 4: Case Reports (12 + 8 = 20 Marks)

Number of Cases: 2 (1 Major & 1 Minor)

Write the diagnosis

Assessment is by Table Viva
SHRI ATAL BIHARI VAJPAYEE MEDICAL COLLEGE & RESEARCH INSTITUTE

Exercise – 5: Viva Voce

Topics: Theory, Application based & Clinical Based

5 Marks for Each Examiner – 4 X 5 Marks = 20 Marks

Response Stations
 Response Station 1
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Identify the apparatus. 1 Mark

2 What is the principle ? 2 Marks

3 Mention any two applications. 2 Marks

 Response Station 2
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Identify the apparatus. 1 Mark

2 What is the principle ? 2 Marks

3 Mention any two applications. 2 Marks

 Response Station 3
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name the two phases in paper chromatography. 1 Mark

2 Mention two types of chromatography. 2 Marks

3 Mention two applications of paper chromatography in medical field. 2 Marks

 Response Station 4
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is the principle of PCR? 2 Mark

2 Mention any two applications. 2 Marks

3 What enzyme is used in PCR? 1 Marks

 Response Station 5
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is the principle of the given apparatus? 1 Mark

2 Mention any two applications. 2 Marks

3 Mention the component of the apparatus. 2 Marks

 Response Station 6
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name two types of ion selective electrodes. 2 Marks
2 Which electrolytes are measured by using ion selective electrode? 1 Mark
3 Write two advantages of using ion selective electrodes. 2 Marks
 Response Station 7
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name the three components measured by electrodes in Arterial Blood Gas Instrument. 2 Marks
2 From which site is the specimen collected for arterial blood gas analysis? 1 Mark
3 Mention the anticoagulant used for collection of specimen for arterial blood gas analysis. 1 Mark
4 Mention the reference range of pH of blood. 1 Mark
 Response Station 8
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name the instrument used to detect specific gravity of urine. 1 Mark
2 Write how temperature correction is done to calculate specific gravity. 1 Mark
3 Write the reference range for specific gravity for normal urine. 1 Mark
4 Mention two clinical conditions associated with increased specific gravity. 1 Mark
5 Mention two clinical conditions associated with decreased specific gravity. 1 Mark
 Response Station 9
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is the normal colour of CSF? 1 Mark
2 Name two clinical conditions associated with absence of glucose in CSF 2 Marks

3 Name two clinical conditions which are associated with pronounced increase in CSF proteins. 2 Marks
 Response Station 10
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is the term used for presence of detectable amount of protein in urine? 1 Mark

2 Name any two ketone bodies. 2 Marks

3 Mention the rate limiting enzyme in ketone body synthesis pathway. 2 Marks
 Response Station 11
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name two factors which influences urine volume. 1 Mark

2 Give reasons why high protein diet gives rise to more acidic urine. 1 Mark

3 Mention the instrument used to measure specific gravity of urine. 1 Mark

4 Write the reference range of specific gravity of normal urine. 1 Mark

5 Write how temperature correction is done to calculate specific gravity. 1 Mark

 Response Station 12
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name two parameters which are estimated in 24 hours urine sample. 1 Mark

2 What instructions are given to the patient for collection of 24 hours urine sample? 2 Marks

3 Name two preservatives for normal urine used to prevent growth of bacteria and moulds. 1 Mark

4 Which colour is imparted to normal urine on consumption of B-complex vitamins? 1 Mark

 Response Station 13
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name the test used to detect urobilinogen in urine. 1 Mark

2 Uric acid is an end product of which metabolism? 1 Mark

3 Mention two disorders associated with decreased excretion of urea in urine. 1 Mark

4 Mention two disorders associated with increased excretion of creatinine in urine. 1 Mark

5 Mention two disorders associated with increased excretion of uric acid in urine. 1 Mark
 Response Station 14
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name two causes for milky or turbid appearance of urine. 1 Mark

2 Name the condition in which imparts fruity odour to urine. 1 Mark

3 What is the term used for increased in urine output of > 3000 ml/day? 1 Mark

4 Mention two clinical conditions associated with increased in urine output. 1 Mark

5 What is the term used for total suppression of urine formation (< 100 ml/day)? 1 Mark
 Response Station 15
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name one hyperglycemic and one hypoglycemic hormone. 1 Mark

2 Explain why Benedict’s test is called semi-quantitave test. 2 Marks

3 Name the term used to mention the presence of glucose in urine. 1 Mark

4 Mention two sugars other than glucose which are excreted in urine. 1 Mark
 Response Station 16
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is your probable diagnosis? 1 Mark

2 Mention its cause. 2 Marks

3 Mention any two clinical features. 2 Marks

 Response Station 17
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is your probable diagnosis? Justify 2 Marks

2 Mention the RDA for the above deficient biological compound. 1 Marks

3 List any two laboratory investigation for above condition. 2 Marks

 Response Station 18
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is the probable diagnosis? 1 Mark

2 What is the main cause for above diagnosis? 1 Mark

3 Which amino acid supplement can reduce for symptoms? 1 Mark

4 Mention the coenzyme of deficient biological compound. 2 Marks

 Response Station 19
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Mention the deficiency manifestations observed in above child photograph. 2 Mark

2 Mention the RDA of the deficient biological compound. 1 Mark

3 Mention the source of the deficient biological compound. 2 Marks

 Response Station 20
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is the probable diagnosis? 2 Mark

2 Mention the safe limit of fluorine. 1 Mark

3 How to prevent mottling of teeth. 2 Marks

 Response Station 21
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Identify the nutritional disorder the child is suffering from? 2 Mark

2 What is balanced diet? 1 Mark

3 Edema is seen in which nutritional disorder in case of children? Justify. 2 Marks

 Response Station 22
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Alb α1 α2 β M γ

Q.No Question Marks

1 What is your probable diagnosis? 1 Mark
2 What are bence jones proteins? 1 Mark
3 Name any two test to detect them in urine. 2 Marks
4 Mention the confirmatory test. 1 Mark
 Response Station 23
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name the enzyme deficiency causing phenylketonuria? 1 Mark
2 Name three alternate/minor pathway metabolites excreted in urine in phenylketonuria. 3 Marks
3 What is the odour of urine in phenylketonuria? 1 Mark
 Response Station 24
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is ochronosis? 1 Mark
2 Name the enzyme deficiency causing alkaptonuria. 1 Mark
3 Mention the reason why urine turns dark on standing in alkaptonuria. 2 Marks
4 Which vitamin supplement will reduce the symptoms? 1 Mark
 Response Station 25
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is the probable diagnosis? 1 Mark

2 Name the enzyme deficiency in above condition. 1 Mark

3 Which amino acid is not synthesised in this condition? 1 Mark

4 Which vitamin coenzyme are involved in this condition? 2 Marks

 Response Station 26
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name two inborn errors associated with fructose metabolism. 2 Marks
2 Name the chemical test done to detect fructose in urine. 1 Mark
3 Name the enzyme deficiency causing galactosemia. 1 Mark
4 Name the chemical test done to detect galactose in urine. 1 Mark
 Response Station 27
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 Name the enzyme deficiency causing hereditary fructose intolerance. 1 Mark
2 Name the enzyme deficiency causing essential fructosuria. 1 Mark
3 Name the enzyme deficiency causing galactosemia. 1 Mark
4 Name the chemical test done to detect galactose in urine. 1 Mark
5 Name the chemical test done to detect fructose in urine. 1 Mark
 Response Station 28
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is your probable diagnosis? 1 Mark
2 List the biochemical parameters elevated in the above condition. 2 Marks
3 Explain biochemical basis for prolonged prothrombin time in this condition. 2 Marks
 Response Station 29
Instruction:
Answer to the point all the questions in stipulated time
Max Marks: 5 Time: 5 Min

Q.No Question Marks

1 What is your probable diagnosis? 1 Mark
2 Explain the biochemical basis of the above condition. 2 Marks
3 Mention the biological reference interval for serum uric acid. 1 Mark
4 Mention the treatment policies of the above condition. 1 Mark
 Response Station 30
Instruction:
Answer to the point all the questions in stipulated time
Max Marks : 5 Time : 5 Min

Q.No Question Marks

1 What is your probable diagnosis? 1 Mark
Mention the enzyme defect in the above condition.
2 2 Marks

What are the signs and symptoms?

3 1 Mark
Mention the treatment policies of the above condition.
4 1 Mark
 Response Station 31
Instruction:
Answer to the point all the questions in stipulated time
Max Marks : 5 Time : 5 Min

Q.No Question Marks

1 What is your probable diagnosis? 1 Mark
What is the cause of the above condition?
2
2 Marks
What are the signs and symptoms?
3 1 Mark

Mention the treatment policies of the above condition.

4 1 Mark
SHRI ATAL BIHARI VAJPAYEE MEDICAL COLLEGE & RESEARCH INSTITUTE

Debalina Sen
Department of Biochemistry
SABVMCRI, Bangalore