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1. Introduction
By 2050, the global population is projected to surpass nine billion individuals (FAO, 2013).
Consequently, the United Nations has recognized the imperative to substantially augment
food production to meet the future population's demands. Notably, the consumption of
animal-derived food is anticipated to rise by 2050, particularly in developing nations, at an
accelerated pace (HPLE, 2016). Conversely, livestock-related activities engender adverse
environmental repercussions, primarily attributable to their substantial contributions to
greenhouse gas emissions and the depletion of critical resources such as land and water (FAO,
2013). Concerning climate change, global greenhouse gas (GHG) emissions have experienced
a resurgence, reaching a record-breaking 52.4 gigatons of carbon dioxide equivalent
(GtCO2e) in 2019 (PNUMA, 2020).
Bill Gates (2021) posits that the agricultural sector accounts for 19% of total GHG emissions,
with livestock production for food constituting the principal contributor within this sector.
However, the author asserts that the primary culprits are not carbon dioxide but rather
methane and nitrous oxide, which possess significantly higher heat-trapping potentials
compared to carbon dioxide, with factors of 28 and 265 times, respectively. Global data
substantiate that livestock production contributes the largest share of GHG emissions within
the agricultural sector, amounting to approximately 65% of emissions, equivalent to nearly
4.6 gigatons of CO2-eq. Beef production alone contributes 2.9 GtCO2e, representing 41% of
the sector's total emissions, while cow's milk accounts for 1.4 GtCO2e, corresponding to 20%
of emissions. Additionally, pork production generates 0.7 GtCO2e, constituting 9% of
emissions. Contributions from milk and meat production of small ruminants amount to 6%,
with the remaining emissions originating from poultry, eggs, other avian species, and inedible
products (FAO, 2013).
Meanwhile, amidst the evolving global landscape, the imperative to explore alternatives for
mitigating greenhouse gas (GHG) emissions becomes increasingly evident. One viable avenue
entails adopting a "sustainable" dietary paradigm that substitutes animal-based meats with
protein alternatives. In this vein, consumers can incorporate diverse sources of alternative
proteins into their diets, such as insect-based protein, plant-based protein, algae, and cell-
based protein sources (ONWEZEN ET AL., 2021). These protein alternatives offer enhanced
health benefits while exhibiting superior environmental sustainability compared to their
animal-derived counterparts (AIKING, 2011).
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2. Methodology
The methodology was carried out in two phases: (2.1) Construction of a portfolio as a source
for collecting articles (PAGANI ET AL., 2015) (2.2) Data collection and analysis procedures
(CORSI ET AL., 2021). As can be seen in Figure 1.
STEPS 6: IDENTIFYING THE VARIABLES: IMPACT FACTOR (IF), CITATION NUMBER (CI) AND PUBLISH YEAR
STEPS 7: RANKING THE PAPERS USING INORDINATIO, RESULTING IN THE FINAL ARTICLE PORTOFOLIO
CONTENT ANALYSIS
Source: Adapted from Pagani et al. (2015; 2018) and Corsi et al. (2021)
Source: Adapted from Pagani et al. (2015; 2018) and Corsi et al. (2021)
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Fortaleza, Ceará, Brasil, 17 a 20 de outubro de 2023.
InOrdinatio= ( 1000
IF
)+α∗[ 10−( ResearchYear−PublishYear ) ] +(∑ c ) i
( 1)
The methodology has nine steps, it is shown in Figure 1 and described below:
Step 1 - Establishment of Research Intent: The objective is to determine the drivers of
acceptance of alternative proteins in South America.
Step 2 - Preliminary research in databases: The combination of keywords with a focus on the
acceptance of alternative proteins was tested in data bases.
Step 3 - Definition and combination of keywords and databases: The databases selected for
this research were Capes, Scopus, and Web of Science, as shown in Frame 1, based on the
study of (ONWEZEN ET AL., 2021).
Frame 1. Combination of keywords and databases for alternative protein in South America
Base Capes, Scopus and Web of Science
Title (Consum* OR Buy* OR Custom* OR Client* OR User*)
All fields (Capes e
Web of Science) (Brazil* OR Argentin* OR Chile* OR Uruguay* OR Paraguay* OR Bolivia* OR Peru*
/Title-abs-key OR Ecuador* OR Venezuela* OR Colombia*)
(Scopus)
All fields (Capes e
Web of Science) (food*) AND (Consumer*) AND (Accept* OR Preference* OR Willing* OR Buy* OR
/Title-abs-key Purchas* OR Choice* OR Behavio* OR Adopt* OR Perception*)
(Scopus)
("Cultured meat*" OR "In vitro meat*" OR "Synthetic meat*" OR Seaweed* OR
All fields (Capes e
Alga* OR Insect* OR Lupin* OR Pulse* OR Legume* OR Bean OR Soy* OR
Web of Science)
Quinoa* OR Corn* OR "Dry pea*" OR Chickpea* OR "Cow pea*" OR "Pigeon pea*"
/Title-abs-key
OR Lentil* OR "Meat alternative*" OR "Meat substitute*" OR "Plant-based meat*" OR
(Scopus)
"Meat analogue*" )
Source: Adapted from Onwezen et al. (2021)
Step 4 - Final search in the databases: Taking into account the various search tools (time, type
of document, search by title, abstract, keywords, and use of Booleans), a total of 183 articles
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XLIII ENCONTRO NACIONAL DE ENGENHARIA DE PRODUÇÃO
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das organizações: Cadeias Circulares, sustentabilidade e tecnologias"
Fortaleza, Ceará, Brasil, 17 a 20 de outubro de 2023.
Step 5 - Filtering procedures: After performing the final search, filtering and elimination
procedures were applied, such as repeated articles; works whose Title, Abstract, Keywords, or
Content are not related to the research topic; Papers presented at conferences and book
chapters. The results obtained in the filtering procedures are shown in Frame 2. The inclusion
and exclusion criteria to this study is shown in Frame 3.
Frame 2. Inclusion and Exclusion criteria (title, abstract, keywords and Content).
Inclusion criteria Exclusion criteria
*Studies carried out in countries of South America. *Articles related to the area of medicine and
*Only articles from academic bases. tourism.
*Studies on consumer behavior, perception, *Studies on veganism, vegetarianism.
acceptance, preferences of alternative protein sources. *Themes of pesticides biofuels, beverages,
*Willingness to purchase or eat products related to packaging, laboratory methods, transgenic
protein alternatives. foods, functional foods, and circular
economy.
Source: Elaborated by the Authors, 2023.
Step 6 - The number of the articles’ citations (Ci) was obtained from Google Scholar; the year
of publication and the Impact Factor (IF) were collected from Scopus and CAPES.
Step 7 - Classification of articles using InOrdinatio: In this research, the Equation InOrdinatio
Equation (1) was applied, giving α the value of 10, considering that the year factor is
important for the study. Thus, resulting in a final portfolio of 13 articles, according to their
scientific relevance.
Step 8 - Search for complete articles: This step was partially carried out in step 6. The 13
articles were found in their complete version, which allowed us to continue with the last step
of Methodi Ordinatio (PAGANI; KOVALESKI; DE RESENDE, 2017, 2015).
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Fortaleza, Ceará, Brasil, 17 a 20 de outubro de 2023.
Step 9 - Final reading and systematic analysis of the articles: After obtaining the final
portfolio with the articles ordered according to their relevance, the systematic reading and
analysis began.
With the portfolio organized and the complete articles, two types of analysis were performed.
Its objective is to contextualize the portfolio of articles, identifying the most influential
authors, the number of publications per year, and recurring keywords. To perform all these
points, the VOSviewer software (V. 1.6.18) was used.
The content analysis aimed to identify the drivers of acceptance for alternative proteins in
South America across the portfolio of articles, using the Nvivo software (V. 12) to easily
identify the common points of each article.
3. Results
The results will be reflected in two sections. The first section presents the bibliometric
analysis and the second corresponds to the content analysis.
After analyzing the articles that met the inclusion and exclusion criteria of frame 4, the final
result of the portfolio was obtained and high-impact articles were found according to its
Inordinatio coefficient that focus on 14 studies of alternative proteins: Cell-based protein (4
studies), planta-based protein (4 studies), Insect-based protein (5 studies) and algae (1
studies).
Based on the final portfolio of articles, an analysis of publications per year was performed, as
shown in Figure 2. It is observed that the year 2021, there was a growing leap, with a greater
number of publications with 46.15% of articles in the portfolio, thus demonstrating that the
subject is currently arousing scientific interest.
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Figure 1. Number of publications per year for alternative protein in South America
Figure 3 shows an authorship and co-authorship map based on bibliographic data. The
network found consisted of 13 authors and 39 co-authors, totaling 11 clusters. It should also
be noted that all authors present an article each, evidencing that there is interest in the topic.
Figure 2. Authorship and co-authorship of the final portfolio for alternative protein in South America
Figure 4 shows the most cited articles in the final portfolio. The article with the highest
citation is: Consumer's willingness to purchase three alternatives to meat proteins in the
United Kingdom, Spain, Brazil, and the Dominican Republic, published in 2019 in the Food
Quality and preference journal, being first in the Inordinatio ranking (Impact factor: 5,565 and
106 citations); followed by the article: First glimpse on attitudes of highly educated
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consumers towards cell-based meat and related issues in Brazil, published in 2019 in PloS one
journal (Impact Factor: 3,24 and 33 citations). Frame 4 details the characteristics of each
article and the InOrdinatio ranking obtained in this review.
The co-occurrence analysis for the main keywords allows us to build Figure 5. The main
keywords are related to the following topics, (1) Types of proteins: Edible insect (4
occurrences), cultured meat (2 occurrences), Quinoa-based protein (1 occurrence), and there
are not keywords related to algae-based protein; (2) Consumer: Consumer behavior (3
occurrences), Consumer attitudes (3 occurrences), Consumer perception (3 occurrences),
Consumer willingness (2 Occurrences), consumer attitudes (2 occurrences) and Consumer
acceptance (2 occurrences); (3) Drivers of acceptance: Animal welfare (3 occurrences), diet (2
occurrences); medicine and health (2 occurrences) and related to nutritional values (2
Occurrences). This analysis shows that all keywords are closely linked, thus stating that our
portfolio appears aligned with our objective. As indicated by Orive, Rio, and Sanchez (2003),
the keywords are valuable to carry out the research and also serve to analyze the works
allowing to discover the direction of the study.
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Figure 4. Overlay visualization of the main keywords found in the final alternative protein portafolio in South
America.
Source: Elaborated by the Authors with a minimum occurrence of two keywords, 2022.
Frame 3. Summary of general aspects found for alternative protein in South America
Protein source Article title Region
Plant-Based protein Consumers’ willingness to purchase three alternatives to meat
Insect-based protein, proteins in the United Kingdom, Spain, Brazil, and the Brazil
Cell-based meat Dominican Republic)
First glimpse on attitudes of highly educated consumers Brazil- Curitiba &
Cell-based meat
towards cell-based and related issues in Brazil Joinville
Who is eating quinoa - How consumer characteristics and
Plant-Based Protein -
beliefs affect the expenditure on this functional food versus Perú - Lima
Quinua
Traditional Staple items
Development and consumer acceptance of gluten-free pasta
Seaweed enriched with Pyropia columbina seaweed. Physical, textural, Argentina
and nutritional properties
Plant-based Protein -
A factor-cluster analysis profile of consumers Perú - Lima
Quinua
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The studies were carried out in the following countries: Brazil (9 articles), Peru (2 articles),
Chile (1 article), and Argentina (1 article). Finally, the 13 articles presented a quantitative
analysis to justify their statements. The drivers for buying this type of protein source are
health (10 articles), taste (7 articles), price (6 articles), safety (5 articles), environmental
aspects (5 articles), nutritional content (5 articles) and others. Frame 5 shows the most
relevant drivers for each type of alternative protein.
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4. Discussion
This section deals with an exhaustive analysis of the results to contextualize them and provide
a rigorous and reasoned interpretation. This critical segment is essential to draw meaningful
conclusions and contribute to the existing body of knowledge in the field of study.
Fernandes et al. (2021) assert that adopting cell-based meat among individuals aged 18 to 39
is strongly driven by considerations pertaining to animal welfare. However, after the age of
40, there is an increase in the number of individuals who would not replace conventional
meat. The author posits that strategies promoting the consumption of laboratory-grown meat
could be formulated based on environmental sustainability and the traceability of drugs used
in its production. Regarding price, young people are more willing to pay for cultured meat
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than conventional meat. Similarly, Paula Soares Valente et al. (2019) emphasize that the
perceived benefits of consuming this type of protein are primarily related to animal welfare,
followed by environmental and health considerations.
Gómez Luciano et al. (2019) find that the most influential drivers for consuming insect-based
protein are nutritional content, health benefits, sustainability, and price. However, consumers
perceive that this protein source is neither tastier nor safer to consume than conventional
meat, possibly due to concerns about disease transmission (Junges et al., 2021). Junges, Do
Canto, and De Barcellos (2021) categorize consumers into two clusters: the first cluster
exhibits no neophobia towards innovative food consumption but low demand and distrust
towards such products, while the second cluster displays a degree of neophobia towards food.
This distinction highlights that a segment of the population is unwilling to consume insect-
based products, whereas another segment shows a keen interest. The identified drivers in this
study encompass neophobia, safety, environmental impact, nutritional value, flavor, product
appearance, price, and packaging.
Similarly, the results of Schardong et al. (2019b) reveal a lack of familiarity and neophobia
concerning the inclusion of edible insects in the diet. Few participants have consumed insects,
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with a notable aversion observed in men compared to women. However, when it comes to
consumption, flour (when not visually present) is the preferred form, followed by whole
insects. La Barbera et al. (2021b) also find that the disgust dimension strongly predicts the
intention to try or use natural and processed insects. The primary drivers identified in their
study are neophobia, taste, and health. Lucchese Cheung et al. (2020) findings indicate that
perceived behavioral control positively influences the intention to consume insects, while
subjective norms and perceived risk serve as negative determinants.
Gómez Luciano et al. (2019) ascertain that health, nutrition, and food safety are primary
motivators for consuming plant-based proteins. Conversely, taste does not exert a significant
influence. This finding is consistent with Dotto et al.'s (2015b) observations, which identify
health benefits as the main reason for purchasing this type of protein, followed by taste. On
the other hand, the primary reasons for abstaining from consuming these foods are habit-
related and personal disinterest. Morales and Higuchi (2022) determine that price and health
are vital in consumer acceptance of plant-based protein. They also highlight the influence of
attitudinal and ethnic identity factors, subjective norms, and experience. In the case of algae-
based protein, flavor emerges as the most influential factor in overall acceptance (Monzon et
al., 2022b).
5. Conclusion
The systematic literature review offers an exhaustive and comprehensive portrayal of the
determinants shaping consumer acceptance of four distinct categories of alternative proteins.
The findings elucidate a marked surge in the number of studies investigating consumer
perspectives on alternative protein sources in recent years, indicating a latent interest in
further exploration of this subject matter. Nevertheless, it is noteworthy that the focal point of
these studies predominantly revolves around Brazil and Peru, thereby indicating that the
compiled drivers of consumer perception through the systematic review may only encapsulate
part of Latin America. Thus, conducting additional studies on consumer perception of
alternative proteins across various Latin American countries is imperative to attain a holistic
understanding of the subject.
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Consumer perception exhibits significant heterogeneity across alternative protein types and is
contingent upon regional dynamics, cultural influences, age demographics, and gender
disparities. Nevertheless, it can be deduced that plant-based proteins elicit a higher degree of
acceptance, followed by cell-based proteins. Conversely, insect-based proteins present more
significant challenges in acceptance due to the persistent presence of neophobia observed in
recent studies. As for algae-based proteins, their position in the consumer acceptance
spectrum remains complex and necessitates further comprehensive exploration.
Based on the precedents mentioned above, the outcomes derived from the systematic review
unveil coherence in the drivers shaping consumer acceptance. It highlights that the most
salient motivators for embracing alternative proteins and influencing food selection are
healthiness and taste, followed by safety, price, and intrinsic characteristics. Furthermore, the
persistence of food neophobia emerges as a noteworthy factor influencing protein choices.
6. Limitations
Finally, it is imperative to acknowledge and address the limitations inherent in this study.
Firstly, it is crucial to note that the identified drivers of consumer acceptance solely pertain to
the specific regions examined and the particular protein variants investigated. Consequently,
the generalizability of these findings to encompass all consumers in South America is not
feasible. Therefore, to achieve a more comprehensive understanding, future quantitative
investigations should be conducted, encompassing diverse types of alternative proteins across
various countries in South America. It will facilitate a more nuanced comprehension of
consumer perceptions and ensure broader applicability of the research outcomes.
7. Declaration of competing interest
The authors assert that they have no competing financial interests or personal affiliations that
have conceivably influenced the scholarly integrity or objectivity of the findings presented in
this manuscript.
Acknowledgments
This study was financed in part by the Coordination of Improvement of Higher Education
Personnel - Brazil (CAPES) - Finance Code 001, and the International Cooperation Group of
Brazilian Universities (GCUB).
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