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Covid Report

The report indicates that Pratik Gupta, a 21-year-old male, tested negative for COVID-19 using a qualitative RT-PCR method on nasopharyngeal and oropharyngeal swabs. All target genes (ORF, N, S) were not detected, and the internal control passed. The report includes notes on the limitations of the test and factors that may influence results, emphasizing that a negative result does not rule out COVID-19 infection.

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16 views1 page

Covid Report

The report indicates that Pratik Gupta, a 21-year-old male, tested negative for COVID-19 using a qualitative RT-PCR method on nasopharyngeal and oropharyngeal swabs. All target genes (ORF, N, S) were not detected, and the internal control passed. The report includes notes on the limitations of the test and factors that may influence results, emphasizing that a negative result does not rule out COVID-19 infection.

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guptapratik770
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Pt's Name : Pratik Gupta Age : 21y Sex : Male

C/o. Self. Lab.No. : 8120114770


Ref.by :
Date/Time of Registration : 03/May/2021 16:34:20

Reference No. :
Collection Time 03/05/2021 16:34

SARS-CoV-2 (COVID-19) QUALITATIVE RT-PCR


Method: Real Time PCR (Qualitative) ICMR Reg.No : GCGLPA
Specimen Type: Nasopharyngeal & Oropharyngeal Swab

* ORF Gene NOT DETECTED


* N Gene NOT DETECTED
* S Gene NOT DETECTED
Internal Control Pass

Conclusion COVID19 NEGATIVE

Comments:
This molecular test uses Real time reverse transcriptase polymerase chain reaction (RT-PCR) technology based on
nucleic acid amplification assay for qualitative detection of RNA of Novel Coronavirus(COVID-19) from Throat and/or
Nasopharyngeal swab, BAL Fluid & sputum samples. The assay includes an internal control with every sample to
check for PCR inhibition. The Limit of detection (LoD) for the three target genes is 10 copies/reaction.

Notes:
• Lower the Ct value (more viral load) higher the risk.
• According ICMR guidelines, It is not recommended to rely on numerical Ct values for determining infectiousness
of COVID-19 patients. Results must be interpreted in conjunction with other clinical and/or laboratory findings.
• According to reports, Ct value above 33 is not contagious.
• Negative result does not rule out the possibility of COVID-19 infection. Improper sample collection technique,
sample collects too early or too late in the course of infection, poor quality of specimen, containing inadequate
patient material, presence of inhibitors in sample, mutations at primer or probe binding sites or insufficient RNA in
patient sample can influence the results.
• False-positive result may be occurred due to cross-contamination from lab environment.
• Repeat sample collection and testing of lower respiratory specimen is advised in case of negative result if
clinically indicated.
• Categories of viral load based on Ct value detected by RT-PCR,

1. Value between 17 to 23 - High viral load


2. Value between 24 to 31 - Moderate viral load
3. Value above 31 – Low viral load
4. Value above 35 – Taken as Negative

-------- End of Report -------

Dr. Umang Patel Dr. Narendra Patel Dr. Kinnari Sodhatar


M.D. (Path.) M.D. (Path. & Bact.) M.D. Microbiology Page No 1 of 1
PDF (Cytogenetics & Molecular Genetics)
03-May-2021 19:41
Reporting Date & Time 03/May/2021 19:41:00

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