Reference: 6702 Technical Data Sheet
Product: BUFFERED PEPTONE WATER ISO 6579, ISO
22964, ISO 6887, ISO 19250, DIN 10181,
Specification
Dilution and non-selective pre-enrichment liquid medium according to ISO standards.
Presentation
2 Prepared Bags /5L Packaging Details Shelf Life Storage
Bags 5L 1 box with 2 bags of 5L. 16 months 8-25 ºC
with: 5000 ± 15 ml PVC plasticizer free sterile bag with: 1 vial stopper + 1 penetrable cap.
Dimensions: 27 x 40 cm. For use in food testing.
Composition
Composition (g/l):
Casein peptone.................................................... 10.0
Sodium chloride................................................... 5.0
Disodium phosphate (anhydrous) ........................3.5(*1)
Potassium phosphate........................................... 1.5
(*1) Equivalent to 9,0 g of disodium
hydrogen phosphate dodecahydrate.
Description /Technique
This formulation of Buffered Peptone Water has the advantages of the two classical diluents used for food samples: it has the property of revitalization of the peptone water and the pH change
absorbing capacity of the phosphate buffer.
The composition of this diluent is made according to the specification of the ISO Standard 6579 for the detection of Salmonella in foods and other ISO Standards (6785, 6887, 8261).
Inoculate according to final purpose, samples and validated methods.
Each Bag is intended for use with an automatic dispenser in laboratories requiring large volumes of broth media or diluent.
Discard any partially used bag to avoid contamination.
The bag has multiple connection points: 1 penetrable cap (injection port) latex-free polycarbonate, for any additive injection required. And an injection (vial stopper) to connect to any standard
equipment laboratory dosing with a connector.
Once completely empty, the bag can be disposed of along with normal plastic (PVC).
Quality control
Physical/Chemical control
Color : yellow pH: 7.0 ± 0.2 at 25ºC
Microbiological control
Prepare tubes/Inoculate 100 ± 20 CFU (productivity)/subculture after holding at 20-25ºC for 45 min. to 1 h, E. coli & Staphyl. Listeria 1h 20± 2ºC.
Microbiological control according to ISO 11133:2014/A1:2018.
Aerobiosis. Incubation at 37 ºC ± 1, reading after 18 ± 2h
Microorganism Growth
®
Stph. aureus ATCC 25923, WDCM 00034 Good. Recovery ±30% T0 (original enumeration)
Escherichia coli ATCC® 25922, WDCM 00013 Good. Recovery ±30% T0 (original enumeration)
Escherichia coli ATCC® 8739, WDCM 00012 Good
Salmonella typhimurium ATCC® 14028, WDCM 00031 Good
Salmonella enterica ATCC® 13076, WDCM 00030 Good
L. monocytogenes ATCC® 13932, WDCM 00021 Good. Recovery ±30% T0 (original enumeration)
Listeria monocytogenes ATCC® 35152 Good. Recovery ±30% T0 (original enumeration)
Escherichia coli ATCC® 8739, WDCM 00012 Good. Recovery ±30% T0 (original enumeration)
Sterility Control
Incubation 48 hours at 30-35 ºC and 48 hours at 20-25 ºC: NO GROWTH.
Check at 7 days after incubation in same conditions.
Page 1 / 2 Revision date: 12/11/20
Reference: 6702 Technical Data Sheet
Product: BUFFERED PEPTONE WATER ISO 6579, ISO
22964, ISO 6887, ISO 19250, DIN 10181,
Bibliography
· ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
. ISO 11133:2014/ Adm 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
· ISO Standard 6579-1 (2017) Microbiology of food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1 : Detection of Salmonella spp.
· ISO 6785 (2001) Milk and milk products. Detection of Salmonella spp.
· ISO 6887-1 (1999) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 1: General rules
for the preparation of the initial suspension and decimal dilutions.
· ISO 6887-2 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 2: Specific rules
for the preparation of meat and meat products.
· ISO 6887-3 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 3: Specific rules
for the preparation of fish and fishery products.
· ISO 6887-4 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 4: Specific rules
for the preparation of products other than milk and milk products, meat and meat products and fish and fishery products.
· ISO/DIS 6887-5 (2009) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 5: Specific
rules for the preparation of milk and milk products.
· ISO 8261 (2001) Milk and milk products. General guidance for the preparation of test samples for microbiological examination.
· ISO 21528-1:2004 Standard. Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1: Detection and enumeration
by MPN technique with pre-enrichment.
· ISO/TS 22964 (2006) Milk and milk products.- Detection of Enterobacter sakazakii
· PASCUAL ANDERSON, Mª R. (1992) Microbiología Alimentaria. Díaz de Santos, S.A. Madrid.
. UNE-EN ISO 11133 (2014). Microbiología de los alimentos para consumo humano, alimentación animal y agua.-Preparación, producción, conservación y ensayos de rendimiento de los medios
de cultivo.
Page 2 / 2 Revision date: 12/11/20
