SANTA CRUZ BIOTECHNOLOGY, INC.
TH (H-196): sc-14007
BACKGROUND RESEARCH USE
The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxy- For research use only, not for use in diagnostic procedures.
genase (TY3H), catalyzes the conversion of tyrosine to L-DOPA, which is the
rate limiting step in the biosynthesis of catecholamines such as dopamine, DATA
adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis
of Parkinson’s disease, which is associated with reduced dopamine levels. A B
A B C
Two transcription factor binding sites in the proximal region of the TH gene, 101 K –
the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), 71 K –
have been implicated in the complex regulation of the TH gene. TH is also < TH
< ∆ TH fusion
49 K –
known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 protein
motif-containing protein called V-1, and a variety of additional compounds. 33 K –
REFERENCES
TH (H-196): sc-14007. Western blot analysis of TH TH (H-196): sc-14007. Immunofluorescence staining of
1. Stull, N.D., et al. 1996. Acidic fibroblast growth factor and catecholamines expression in rat adrenal gland (A) and mouse brain methanol-fixed PC-12 cells showing cytoplasmic locali-
(B) tissue extracts and truncated human recombinant zation (A). Immunoperoxidase staining of formalin fixed,
synergistically up-regulate tyrosine hydroxylase activity in developing and TH fusion protein (C). paraffin-embedded human kidney tissue showing
damaged dopamine neurons in culture. J. Neurochem. 67: 1519-1524. granular cytoplasmic staining of cells in tubules (B).
2. Nagatsu, T., et al. 1998. Catecholamine synthesis and release. Overview
Adv. Pharmacol. 42: 1-14. SELECT PRODUCT CITATIONS
1. Chao, H.J., et al. 2005. The conditioned enhancement of neutrophil activity
CHROMOSOMAL LOCATION is catecholamine dependent. J. Neuroimmunol. 158: 159-169.
Genetic locus: TH (human) mapping to 11p15.5; Th (mouse) mapping to 7 F5. 2. Milosevic, J., et al. 2005 . Low atmospheric oxygen avoids maturation,
senescence and cell death of murine mesencephalic neural precursors.
SOURCE J. Neurochem. 92: 718-729.
TH (H-196) is a rabbit polyclonal antibody raised against amino acids 1-196 3. Costa, C., et al. 2008. Electrophysiology and pharmacology of striatal
of TH of human origin.
neuronal dysfunction induced by mitochondrial complex I inhibition. J.
Neurosci. 28: 8040-8052.
PRODUCT
Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide 4. van Roon-Mom, W.M., et al. 2008. Mutant huntingtin activates Nrf2-
and 0.1% gelatin. responsive genes and impairs dopamine synthesis in a PC12 model of
Huntington’s disease. BMC Mol. Biol. 9: 84.
APPLICATIONS 5. Gauthier, M.A., et al. 2008. Covalent arylation of metallothionein by oxidized
TH (H-196) is recommended for detection of TH of mouse, rat and human ori- dopamine products: a possible mechanism for zinc-mediated enhan-cement
gin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), of dopaminergic neuron survival. Neurotox. Res. 14: 317-328.
immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell ly- 6. Rana, O.R., et al. 2009. Regulation of nerve growth factor in the heart: the
sate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500), role of the calcineurin-NFAT pathway. J. Mol. Cell. Cardiol. 46: 568-578.
immunohistochemistry (including paraffin-embedded sections) (starting dilu-
tion 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 7. Rana, O.R., et al. 2010. Mechanical stretch induces nerve sprouting in rat
1:30, dilution range 1:30-1:3000). sympathetic neurocytes. Auton. Neurosci. 155: 25-32.
Suitable for use as control antibody for TH siRNA (h): sc-36662, TH siRNA (m): 8. Paillé, V., et al. 2010. Distinct levels of dopamine denervation differential-
sc-36661, TH shRNA Plasmid (h): sc-36662-SH, TH shRNA Plasmid (m): ly alter striatal synaptic plasticity and NMDA receptor subunit composi-
sc-36661-SH, TH shRNA (h) Lentiviral Particles: sc-36662-V and TH shRNA (m) tion. J. Neurosci. 30: 14182-14193.
Lentiviral Particles: sc-36661-V. 9. Clewes, O., et al. 2011. Human epidermal neural crest stem cells (hEPI-
Molecular Weight of TH: 60 kDa. NCSC)-characterization and directed differentiation into osteocytes and
melanocytes. Stem Cell Rev. 7: 799-814.
Positive Controls: mouse brain extract: sc-2253, rat adrenal gland extract:
sc-364802 or PC-12 cell lysate: sc-2250. 10. Collo, G., et al. 2012. Pre-synaptic dopamine D3 receptor mediates cocaine-
induced structural plasticity in mesencephalic dopaminergic neurons via
STORAGE ERK and Akt pathways. J. Neurochem. 120: 765-778.
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
